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Citations for Promega :
1251 - 1300 of 2342 citations for 4 Methoxy 6 methyl 6 phenyl 5H pyran 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The indicated DNA oligonucleotide and oligonucleotide ‘HDVrt’ were mutually extended upon one another using three thermocycles with GoTaq Hot-start DNA polymerase (Promega). This generated a double-stranded DNA comprising the DNA sequence of the desired RNA oligonucleotide ...
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bioRxiv - Microbiology 2022Quote: Cell viability was measured using a commercially available kit [CellTiter 96 Aqueous One Solution Cell Proliferation Assay (Promega, Madison, USA)] ...
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bioRxiv - Zoology 2022Quote: ... The sperm suspension was then divided into two samples: one for determining ATP using a CellTiter-Glo Luminescent Cell Viability Assay kit (Promega) and the other for assessing DNA content (to calibrate the ATP content by the number of sperm cells ...
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bioRxiv - Synthetic Biology 2024Quote: ... Four microliters of CFPS sample were added to 30 μL of ONE-Glo Luciferase Assay System (Promega, Madison WI, USA) in a white 96-well plate (Costar 3693 ...
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bioRxiv - Plant Biology 2024Quote: ... total RNA was extracted from one week-old Arabidopsis seedlings and reverse transcribed into cDNA using M-MLV reverse transcriptase (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Genomic DNA was isolated and purified using the Wizard Genomic DNA Purification Kit protocol for Gram negative bacteria at one-third volume (Promega), then quantified using SYBR Green I Nucleic Acid Stain (Invitrogen ...
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Phospholipase C β4 promotes RANKL-dependent osteoclastogenesis by interacting with MKK3 and p38 MAPKbioRxiv - Cell Biology 2024Quote: ... the existing media was removed and replaced with 100 µL of medium containing 20 µL of CellTiter 96 AQueous One solution (Promega). The cells were then incubated for 4 h at 37°C in a humified atmosphere with 5% CO2 ...
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bioRxiv - Genomics 2024Quote: ... The final DNA yield (17.3 µg) was quantified using a Quantus Fluorometer (QuantiFluor ONE dsDNA Dye assay; Promega, Madison, WI). The size distribution of the HMW DNA was estimated using the Femto Pulse system (Agilent ...
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bioRxiv - Bioengineering 2024Quote: ... Cell viability and in vitro luciferase expression was evaluated via ONE-Glo + Tox luciferase reporter and cell viability assay kit (Promega) and a multimode microplate reader.
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bioRxiv - Microbiology 2023Quote: ... were infected with VSV-G-pseudotyped NL4-3Δenv-luc at a MOI of 0.0001 and Luciferase expression was quantified after two days using the One-Glo™ luciferase assay (Promega) as described previously [8].
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bioRxiv - Microbiology 2022Quote: ... allowing to adhere for 24 h before addition of a serial dilution of inhibitor and measurement of cell viability 72 h later using the CellTiter AQueous One solution (Promega), following manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Cell viability was assessed using a Cell Titer 96 AQueous One Solution Cell Proliferation Assay (Promega Corp, Madison, Wisconsin, USA). Absorbance measurements at 490 nm were normalized to the maximum read per cell line ...
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bioRxiv - Molecular Biology 2022Quote: ... Next two overlapping fragments containing one of the homologies and a part of the antibiotic marker were generated by PCR using Taq polymerase (Promega), which overlap for a length of 594 bp ...
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bioRxiv - Microbiology 2022Quote: ... allowing to adhere for 24 h before addition of a serial dilution of protease inhibitors and measurement of cell viability 72 h later using the CellTiter AQueous One solution (Promega), following manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... Equal amounts of the lysate were transferred into two Microtiter plates (one for Renilla and the second for Bright Glo (Promega)) ...
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bioRxiv - Neuroscience 2023Quote: LDH release from macrophages treated with Aβ profect complexes was measured using the CytoTox-ONE Homogeneous Membrane Integrity Assay (cat no: G7891, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Cell proliferation was assessed every 24 h by the MTS assay (CellTiter 96® AQueous One Solution Cell Proliferation, Promega). DMSO-treated cells were used as negative controls and the effects on cell proliferation were expressed as the percentage inhibition compared to the cells of the control group ...
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bioRxiv - Systems Biology 2023Quote: Cell viability was measured using a commercial kit (Cell Titre 96 AQueous One Solution cell proliferation assay; Promega, Madison, USA) that uses a tetrazolium salt-based colorimetric assay [20] ...
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bioRxiv - Developmental Biology 2023Quote: ... After 7 days of culture the MTS cell viability reagent (CellTiter 96® Aqueous One Solution Cell Proliferation Assay, Promega) was added and plates incubated for 4 hours at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: Cell proliferation colorimetric-based assays were performed using 4,000 transduced or transfected cells and the CellTiter 96 Aqueous One Solution Cell Proliferation Assay (Promega, G3582) according to the manufacturer’s instructions for 96h ...
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bioRxiv - Microbiology 2024Quote: ... Cell viability was measured using a commercially available kit [CellTiter 96 Aqueous One Solution Cell Proliferation Assay (Promega, Madison, USA)] ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The total RNA of each species was extracted from male and female nymphs (each one individual, whole body) using ISOGEN □ (Nippon Gene) and ReliaPrep RNA Tissue Miniprep Systems (Promega). Total RNA extracted from a male and female nymph was mixed in equal amounts ...
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bioRxiv - Synthetic Biology 2024Quote: ... 0.06pmol of donor backbone and 0.04pmol of All-in-One backbone were co-transfected in HEK293T using FuGENEHD (FuGENE® HD Transfection Reagent, Promega) as transfection reagent at a ratio of 3:1 FuGENEHD (μL) ...
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bioRxiv - Microbiology 2024Quote: ... The bacterial cultures were then aliquoted into Falcon 96-well white plates (100 µL/well) and mixed with one volume of BacTiter-Glo reagent (Promega), followed by incubation in the dark on a rocker with gentle shaking at room temperature for 20 min ...
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bioRxiv - Molecular Biology 2024Quote: ... The proliferation curve was built by harvesting one plate every 24 hours and performing the Cell Titer Glo (Promega, G7572) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... and NIC-PS on the growth of HepG2 and Huh7 cells were assessed using the CellTiter 96 Aqueous One Solultion Cell Proliferation assay (cat# G4100, Promega). Cells were seeded at 3×103 cells per well in 96-well plates and allowed to adhere overnight ...
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bioRxiv - Microbiology 2021Quote: ... 2 μg RNA was incubated at 30 °C for 2 hr in Spodoptera frugiperda (Sf21) extract (Promega) in the presence of [35S]methionine-cysteine (Perkin-Elmer ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were transfected with 20 ng/well of psiCHECK-2 plasmid (psiCHECK-2 Vector (V0) (Promega, C8021) or let-7a-mi6 targeting six regions of the 3ill UTR ...
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bioRxiv - Cell Biology 2022Quote: ... β-arrestin 2 fused at the N-terminus to LgBiT (LgBiT-β-arrestin 2; Promega, plasmid no. CS1603B118) was chosen as it has previously been used successfully with other class B GPCRs ...
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bioRxiv - Plant Biology 2021Quote: ... the protein suspensions were digested with 4 μg trypsin (Promega) in 40 μl TEAB buffer overnight at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... 25 μL of 4 mM luciferin (Assay Reagent, Promega, E1291) supplemented with isoproterenol at a final concentration of 200 nM was added ...
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bioRxiv - Microbiology 2023Quote: ... the protein suspension was digested with 4 μg trypsin (Promega) in 40 μL NH4HCO3 (50 mM ...
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bioRxiv - Neuroscience 2023Quote: ... After embedding in 4% low-melting point agarose (Promega, #V2111) in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... and then 4 hours in mass-spectrometry-grade trypsin (Promega). The missed cleavage rate for samples was an average of 3.9%-21.9% ...
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bioRxiv - Cell Biology 2020Quote: ... The caspase-3/7 activity of the lysates or the plated cells was analysed using Apo-ONE ® Homogenous Caspase-3/7 Assay (Promega) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... medium was changed and cellular expression of luciferase reporter indicating viral infection was determined using ONE-Glo™ Luciferase Assay System (Promega) in 48 hr after infection ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 ul of this mixture was added 25 ul of the ONE-Glo™ EX reagent following 25 ul of NanoDLR Stop & Glo reagent (Promega). All mixtures were incubated in opaque white 96-well plates on a rocking shaker in the dark for 5 min before quantifying the luminescence.
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bioRxiv - Genomics 2020Quote: ... TRs were amplified using the forward and reverse primers plus an M13 primer with one of the four fluorophores with GoTaq polymerase (Promega #PRM7123) using PCR program ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were incubated with nanoparticles encapsulating Fluc mRNA and analyzed for cell viability and luciferase activity with the ONE-Glo™+Tox luciferase reporter and cell viability assay kit (Promega) using a multimode microplate reader.
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bioRxiv - Neuroscience 2020Quote: ... CellTiter 96® Aqueous One Solution cell proliferation assay kit and ApoTox-Glo™ Triplex Assay kit was obtained from Promega, Australia ...
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bioRxiv - Cell Biology 2021Quote: ... or without (150 bp) one putative ETV2 binding site (Wei et al, 2010) were cloned into pGL3_Basic vector (Cat# E1751, Promega, Madison, WI). Primers Forward ...
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bioRxiv - Microbiology 2021Quote: ... The migrated cells in the lower chamber were stained with 5 μl of CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega # G3580) for 2 h at 37° C with 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... Cell viability was measured 48h later (or otherwise indicated) using CellTiter 96® AQueous One Solution Cell Proliferation Assay (G3582, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... After 24 hours of growth luciferase activity was visualized by spraying one spray of 1:100 reconstituted NanoGlo Luciferase substrate (Promega N1110) per agar plate using a disposable spray flask ...
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bioRxiv - Cell Biology 2020Quote: ... and results were normalized to living cell numbers as evaluated with the CellTiter 96® AQueous One Solution Cell Proliferation Assay Kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The virus-serum mix was subsequently used to infect A549-hACE2-TMPRSS2 cells for 18 hr at 37 Celsius before adding ONE-Glo reagent (Promega E6120) for measurement of luciferase signal (relative luminescence unit ...
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bioRxiv - Molecular Biology 2021Quote: ... and another 25 μL of cell lysate was mixed with 25 μL of ONE-Glo Luciferase Assay System (Promega, Cat.# E6130). The plate was placed on a shaker for 5 min in the dark ...
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bioRxiv - Bioengineering 2022Quote: ... The cell proliferation assay was conducted with CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) reagent (Promega, Cat.No. G3582) following the instruction ...
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bioRxiv - Neuroscience 2023Quote: Cytotoxicity was assessed by measuring the lactate dehydrogenase (LDH) release from cells with damaged membranes using the CytoTox-ONE Homogeneous Membrane Integrity assay (#7891, Promega, USA). For this ...
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bioRxiv - Microbiology 2023Quote: ... One aliquot was used for DNA extraction using the Promega Wizard® Genomic DNA Purification kit (Promega Corporation, Madison, WI, USA) as per the manufacturer’s protocol ...