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Citations for Promega :
1301 - 1350 of 5154 citations for Rat Presenilin Enhancer 2 Homolog C. Elegans PSENEN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Pseudoviruses stock were titrated by infecting 293T-ACE2 cells and luciferase activity was determined following a 44-48 h incubation period at 37°C and 5% CO2 by addition Bright-Glo Luciferase Assay System (Promega, E2650) using a microplate reader (TECAN ...
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bioRxiv - Cell Biology 2022Quote: ... and proteins were subjected to on-cartridge digestion with mass spec grade Trypsin/Lys-C Rapid digestion enzyme (Promega, Madison, WI) at 70°C for 1 hour ...
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Revisiting the role of Toxoplasma gondii ERK7 in the maintenance and stability of the apical complexbioRxiv - Microbiology 2021Quote: ... Overnight digestion was performed at 37 °C with 5 μl of freshly prepared trypsin (Promega; 0.2 μg/μl in TEAB 0.1M). To remove RapiGest ...
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bioRxiv - Systems Biology 2020Quote: ... Samples were dried in a vacuum centrifuge and resuspended in 200 uL of 0.05 ug/uL Lys-C/Trypsin (Promega, WI, USA) in 25 mM Ammonium Bicarbonate ...
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bioRxiv - Microbiology 2020Quote: ... Cells were further cultured for 72 h at 37°C with 5% CO2 and lysed in luciferase substrate buffer (Bright-Glo; Promega, Poland). Lysates were transferred onto white 96-wells plates and luciferase levels were measured on a microplate reader Gemini EM (Molecular Devices ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were then reduced with 10 mM DTT in 100 mM Ambic for 30 min at 40 °C and alkylated with 55 mM iodoacetamide in 100 mM Ambic for 20 min at 40 °C followed by digestion with 0.3 μg trypsin (sequence grade, Promega, Madison, WI) in 50 mM Ambic for 5 h at 40 °C ...
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bioRxiv - Microbiology 2020Quote: ... samples were heated at 80 °C for 15 min and cooled to RT before addition of 1 μg of trypsin (Promega, V511A). Samples were digested at 37 °C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were alkylated using 15 mM chloroacetamide at 37° C for 30 min and further digested using sequencing-grade modified trypsin (1/50 w/w, ratio trypsin/protein; Promega, USA) at 37° C for 12 hours ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... The urea concentration was reduced to <2M and the protein sample was digested overnight at 37°C with 1:100 Trypsin Gold (Promega V5280). The following day ...
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bioRxiv - Immunology 2022Quote: The Flag-TRAF6 preparations eluted from FLAG resin with 3X FLAG peptide were digested with a combination of trypsin and Lys-C protease (Promega, #V5071) using S-trap mini columns (Protifi ...
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bioRxiv - Immunology 2022Quote: ... Proteins then were reduced with 10 mM DTT in 100 mM Ambic for 30 min at 40oC and alkylated with 55 mM iodoacetamide in 100 mM Ambic for 20 min at 4°C followed by digestion with 0.3 mg trypsin (sequence grade, Promega, Madison, WI) in 50 mM Ambic for 5 h at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... The gel pieces were dried with 200 μl of ACN and digested with 10 ng/μl Lys-C (sequencing grade, Promega, USA) in 50 mM NH4HCO3 overnight at 37 °C (14).
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bioRxiv - Molecular Biology 2022Quote: ... Proteins then were reduced with 10 mM DTT in 100 mM Ambic for 30 min at 40°C and alkylated with 55 mM iodoacetamide in 100 mM Ambic for 20 min at 40°C followed by digestion with 0.3 μg trypsin (sequence grade, Promega, Madison, WI) in 50 mM Ambic for 5 hours at 40°C ...
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bioRxiv - Bioengineering 2024Quote: ... were taken for overnight digestion with mass spec grade Trypsin/Lys-C Mix (Promega, Madison, WI, cat. No. V5071, 1:20). Samples were reduced with TCEP (final concentration 5 mM ...
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bioRxiv - Biochemistry 2024Quote: ... when the temperature was dropped to 22°C and isopropyl β-d-1-thiogalactopyranoside (IPTG) and L-rhamnose (Promega, Madison, WI) was added at 0.2 mM and 0.1% (w/v ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was diluted 5-fold with 50 mM Tris-HCl pH 8.0 and digested for 12 h at 37°C using sequencing grade trypsin (Promega cat#: V511A) at 1/100 the mass of total protein ...
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bioRxiv - Biophysics 2023Quote: ... each respective cell type was incubated at 37 °C with 500 pM of Janelia Fluor® 646 HaloTag Ligand (NSCs, ECs, or keratinocytes) (Cat. No. GA1120, Promega) or Janelia Fluor® 635 HaloTag Ligand (MNRs ...
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bioRxiv - Cell Biology 2024Quote: ... images produced from HeLa cells seeded on glass-bottom Mattek dishes (P35GC-1.5-14-C) and transfected with TFG-mEGFP-FLAG at 1 µg/µL using Fugene (Promega, cat # PRE2311) for 24 hours ...
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bioRxiv - Immunology 2023Quote: ... 25 ng of c-DNA was used per reaction in each well containing the 2X SYBR green PCR master mix (Promega, USA) along with appropriate primers ...
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bioRxiv - Immunology 2022Quote: ... The samples were subsequently quenched with 10 mM DTT with overnight digestion at 37°C using Trypsin gold (Promega, WI, USA). The digestion was terminated and the surfactant was cleaved by treating the samples with 200 mM HCl (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... at an enzyme-to-substrate ratio of 1:100 and subsequently digested overnight at 37°C with sequencing grade trypsin (Trypsin Gold, Mass Spec Grade, Promega, V5280) at an enzyme-to-substrate ratio of 1:50 ...
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bioRxiv - Molecular Biology 2023Quote: ... and MSN(T558A) genes with either N-terminal or C-terminal SmBit and LgBiT fusions were produced using Flexi®-based cloning (Promega). Best luminescence signals were obtained with CD44 containing a C-terminal smBiT fusion (C-terminal smBiT ...
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bioRxiv - Immunology 2023Quote: ... Plates were incubated for 22 hours at 37 °C/5 % CO2 prior to addition of 33ul Bio-Glo reagent (Promega G7940), then luminescence was read on a Clariostar plate reader.
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bioRxiv - Cell Biology 2023Quote: ... samples were resuspended in 50 µl of 50 mM ABC buffer and digested at 37°C for 16 h using 1 µg of trypsin (Trypsin Gold, Promega, V5280) and 1 µg of LysC (Wako Pure Chemical Corporation ...
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bioRxiv - Immunology 2023Quote: ... 25 ng of c-DNA was used per reaction in each well containing the 2X SYBR green PCR master mix (Promega, USA) along with appropriate primers ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were alkylated using 15 mM chloroacetamide at 37° C for 30min and further digested using sequencing-grade modified trypsin (1/50 w/w, ratio trypsin/protein; Promega, USA) at 37°C for 12h ...
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bioRxiv - Cell Biology 2023Quote: ... incubated 3 h at 37°C and alkaline phosphatase (SEAP) activity determined measuring the absorbance at 600 nm using GloMax®-Multi Detection System (Promega).
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bioRxiv - Cell Biology 2023Quote: ... 30 min) and diluted to 1M Urea with 200 mM ABC for trypsin digestion (1 μg, 37°C, overnight shaking, Promega, #V5113). On the next day ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was cleared by centrifugation at 15,000 g for 15 min at 4°C and 45 μL of the supernatant were mixed with an equal volume of the NanoLuc Reagent (Promega N1110) prepared according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... for 90 min at 37°C (enzyme-to-substrate ratio of 1:100) and subsequently digested overnight at 37°C with trypsin (Trypsin Gold, Mass Spec Grade, Promega, V5280) at an enzyme-to-substrate ratio of 1:50 ...
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bioRxiv - Cancer Biology 2023Quote: ... Reactions were incubated for 1 hr at 30 °C and luciferase signal was measured using Dual-Glo Luciferase Assay System (Promega E2920).
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bioRxiv - Systems Biology 2024Quote: ... protein digestion was performed overnight by addition of 50 nL of 0.01 ng nL-1 Lys-C (MS grade, Promega, Madison, USA) and 50 nL of 0.04 ng nL-1 trypsin (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were digested at 47 °C for two hours with 600 ng of mass spectrometry grade Trypsin/LysC mix (Promega, V5113). The digested peptides were then eluted with two 35 µl increments of 0.2% formic acid in water and two more 40uL increments of 80% acetonitrile with 0.2% formic acid in water ...
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bioRxiv - Biochemistry 2023Quote: ... samples were resuspended in 6 M guanidine hydrochloride.250 µg of protein were digested overnight at 37 °C using trypsin with a 1:20 trypsin to sample ratio (Promega, #V5117). The next day ...
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bioRxiv - Microbiology 2023Quote: ... Beads were incubated overnight at 37°C with 20 μL of 25 mM NH4HCO3 buffer containing 0.2µg of sequencing-grade trypsin (Promega, Madison, WI, USA). The resulting peptides were loaded and desalted on evotips provided by Evosep (Odense ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and a final extension at 72 °C for 10 min using GoTaq® G2 Hot Start Green Master Mix (Promega, USA). The PCR products of each gene were then separated on 2% agarose gels containing SYBR® Safe DNA Gel Stain (ThermoFisher Scientific) ...
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bioRxiv - Bioengineering 2023Quote: ... and loaded on a micro S-trap column (Protifi, NY) and digested according to vendor protocol for 1 hour at 50 C by Trypsin Platinum (Promega, WV). Digested material was then eluted from the column by centrifuge at 1000 rpm for 2 minutes and dried in speedvac (Eppendorf ...
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bioRxiv - Physiology 2024Quote: ... Protein (50 μg per sample) was digested overnight at 37°C with LysC (Wako Chemicals) at 1:50 (w/w) ratio and trypsin (Promega, V5113) at 1:100 (w/w ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Seventy micrograms of protein were digested overnight using the filter-aided sample preparation (FASP) method with Trypsin/Lys-C mix (Promega, USA). Next ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were digested at 47 °C for two hours with 600 ng of mass spectrometry grade Trypsin/LysC mix (Promega, V5113). The digested peptides were then eluted with two 35 µl increments of 0.2% formic acid in water and two more 40uL increments of 80% acetonitrile with 0.2% formic acid in water ...
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bioRxiv - Biochemistry 2024Quote: ... The well plates were incubated at 30 °C and the optical density at 560 nm was measured using a plate reader (Promega ProMax). We defined a growth inhibition of at least 50 % in comparison to the growth in the DMSO-supplemented medium as the cut-off to determine the MIC.
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bioRxiv - Microbiology 2024Quote: Proteins were digested for 11 hours at 37°C under agitation at 600 rpm on a ThermoMix (Thermo) using Sequencing Grade Modified Trypsin (Promega - V5111) with a 1:50 ratio (enzyme:protein ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then pelleted and disrupted by glass bead agitation at 4°C in 1x Passive Lysis Buffer provided in the Dual-Luciferase Reporter Assay System (Promega #E1910). Extracts were clarified by centrifugation ...
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bioRxiv - Plant Biology 2021Quote: ... Genomic DNA was extracted using a DNA extraction kit (Wizard Genomic DNA Purification Kit; Promega Corp, Madison, WI). Genomic DNA was extracted from 1-week old bacterial sub cultures by collecting a loop of bacteria with a sterile pipette tip and inoculating a 0.2 ml PCR tube containing 15 μl of Molecular Grade Water and ran in a thermal cycler for 15 minutes at 95 °C.
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bioRxiv - Plant Biology 2023Quote: ... multiflorum samples using a commercial DNA extraction kit (Wizard HMW DNA Extraction Kit, Promega Corporation, Madison, WI, USA).
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bioRxiv - Cancer Biology 2021Quote: ... 2 µg of RNA was subjected to reverse transcription using M-MLV enzyme (Promega #M1705), dNTP mix 100 mM each (BLIRT #RP65 ...
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bioRxiv - Microbiology 2020Quote: ... Protein digestion was carried out by adding 2 μg of trypsin solution (Promega, Charbonnières, France) to the alkylated proteins ...
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bioRxiv - Molecular Biology 2019Quote: ... The linearized plasmid was treated with 2 units of shrimp alkaline phosphatase (Promega, Madison, USA) at 37 °C for 30 min ...
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bioRxiv - Bioengineering 2021Quote: ... and 1 and 2 μL of 50 ng/μL trypsin (in 100 mM TEABC, Promega) was added to the wells with single cells and two hundred carrier cells ...
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bioRxiv - Cell Biology 2021Quote: ... treated with 3 units/sample of DNase I for 2 hours (Promega, Madison, WI, USA), and 1-3μl aliquots were taken for reverse transcription (RT ...