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Citations for Promega :
1151 - 1200 of 5154 citations for Rat Presenilin Enhancer 2 Homolog C. Elegans PSENEN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... anti-m6A conjugated beads were incubated with purified mRNA with rotation at 4°C overnight in 300 µL MeRIP buffer with 1 µL RNase inhibitor (recombinant RNasin; Promega). 10% of the mRNA sample was saved as the input fraction ...
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bioRxiv - Bioengineering 2020Quote: ... In-solution protein digestion was carried out in a ratio of 1:25 w/w Trypsin/Lys-C (Mass Spectrometry Grade, Promega) to protein overnight at 37°C ...
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Quantitative proteomics reveals remodeling of protein repertoire across life phases of Daphnia pulexbioRxiv - Systems Biology 2019Quote: ... pH 8.5 and digested in a two-step process starting with an overnight digest at room temperature with Lys-C (Wako) followed by six hours of digestion with trypsin (sequencing grade, Promega) at 37 ° C ...
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bioRxiv - Synthetic Biology 2019Quote: ... Backbone and inserts were ligated either for 3 h at RT or overnight at 4°C using T4 ligase (Promega). All vector sequences (Table S5 ...
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bioRxiv - Biochemistry 2019Quote: ... Reactions (3 μL) were incubated for 60 min at 37 °C and quantified with the Nano-glo assay system (Promega).
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bioRxiv - Genomics 2019Quote: ... Tagmentation was performed on 250,000 HCT116 Parental and DKO cells using 700nM Tn5 Transposase assembled using preannealed Tn5ME-A_mC and Tn5ME-B_mC (Table S3) for 30 minutes at 37°C following the addition of 0.01ng of unmethylated Lambda DNA (Promega #D1521). We recommend performing a titration of Tn5 transposase to nuclei input to assay minimum amounts required as in Figure S5 ...
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bioRxiv - Pathology 2020Quote: ... Sample pH was adjusted to 7.5 with 0.05 M TEAB and proteins were digested overnight at 37 °C with sequencing-grade trypsin (Promega, Madison, WI) in 1:30 (w/w ...
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bioRxiv - Biophysics 2021Quote: ... Samples were incubated at 25°C for 30 minutes with gentle shaking before addition of 50 µL Kinase GloTM Max reagent (Promega) and further incubation at 25°C for 10 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... The sequences ligated by the T4 ligase at 4 °C for 12 h were cloned in the pGEM T-easy vector (Promega). The competent Escherichia coli X10-Gold (Stratagene ...
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bioRxiv - Biochemistry 2020Quote: ... cDNAs were obtained from Origene in pCMV6 vector that also incorporates C-terminal Myc and FLAG tags (Myc-FLAG) or subcloned into a pHTC (Promega)-derived vector that expresses C-terminal HaloTag (41) ...
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bioRxiv - Cell Biology 2021Quote: ... alkylated with 5 mM iodoacetamide for 20 min at room temperature and digested overnight at 37 °C using 13 ng/μl trypsin (Promega). Digested peptides were loaded onto C18-Stage-tips (Rappsilber et al. ...
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bioRxiv - Plant Biology 2020Quote: ... The soluble fraction was then digested for 20 h at 37°C using a 1:50 ratio of trypsin (Promega) to extracted protein while gently shaking ...
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bioRxiv - Microbiology 2021Quote: ... After 30 min at 60°C the samples were cooled to room temperature and 4 μg of Sequencing Grade Trypsin (Promega) for digestion were added ...
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bioRxiv - Microbiology 2021Quote: ... samples were washed-dried once again and digested overnight at 37°C using mass spectrometry grade Trypsin gold (Trypsin Gold, Promega) at a concentration of 12.5 ng/μl in 50 mM ammonium bicarbonate and 10 mM CaCl2 ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then left at 32°C or moved to 37°C for 9 hours before read on a luminometer using the Dual-Luciferase Reporter Assay Step (Promega) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Plates were frozen for at least 30 minutes at −80°C before thawing and reading on a GloMax Multi plate reader (Promega). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... which were washed with a series of harsh washes before cross-linker reversion and on bead digest with Trypsin Lys-C (Promega). Peptides were then desalted and prepared for mass spectrometry ...
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bioRxiv - Cell Biology 2021Quote: ... while the sequences for NSD2 or NSD3 PWWP1 domain was cloned in-frame into a pNLF1-C [CMV/Hygro] vector from Promega using the In-Fusion HD Cloning kit (Takara) ...
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bioRxiv - Cell Biology 2021Quote: ... NY) and washed with S-Trap buffer prior to overnight proteolysis at 37°C with LysC/trypsin (Promega, Madison, WI) in 50 mM ammonium bicarbonate (pH 8.0 ...
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bioRxiv - Neuroscience 2020Quote: ... 8 min, 4 °C) and resuspended in staining buffer (PBS, pH 7.4, with 0.5% RNase-free BSA (Promega, Heidelberg, Germany) and 0.2 U/μl of RNasin Plus RNase inhibitor (Promega)) ...
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bioRxiv - Cell Biology 2020Quote: ... and digested overnight at 37°C with 1:50 enzyme:substrate ratio (wt/wt) of sequencing grade trypsin (Promega, Madison, WI) as described 59 ...
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bioRxiv - Cell Biology 2021Quote: ... for 1 hour at 37°C and DNA was extracted using the Wizard SV gel and PCR cleanup system (Promega). Input and immunoprecipitated DNA was subjected to quantitative PCR using the oligonucleotides shown in Table 1 ...
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bioRxiv - Cell Biology 2021Quote: ... Trypsin digestion was carried out overnight at 37°C with 1:50-1:100 enzyme-protein ratio of sequencing grade-modified trypsin (Promega) in 50 mM NH4HCO3 pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins on beads were digested overnight at 37°C by sequencing grade trypsin (12.5 μg/ml; Promega Madison, Wi, USA) in 20 μl of NH4HCO3 25 mmol/l ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were diluted with ddH2O 1:5 and proteins were digested for 20 h at 37°C using trypsin (Trypsin Gold, Promega) at an enzyme/protein ratio of 1:50 ...
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bioRxiv - Cell Biology 2022Quote: ... The cell plates were incubated at 37°C for 48-60 hours before 50 μL of CellTiter-Glo reagent (Promega) was added ...
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bioRxiv - Cell Biology 2022Quote: ... for 2 h followed by a second digest with trypsin at 35°C overnight at 1200 rpm (1:50 ratio w/w, Promega). Proteolysis was stopped by the addition of 1% (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... Trapped proteins were washed four times with the methanol TEAB buffer and then digested overnight at 37°C using 1ug Trypsin (Promega). Digested peptides were eluted and dried in a vacuum centrifuge before LC-MS analysis.
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bioRxiv - Immunology 2022Quote: ... pH 8.0 using Vivaspin columns with a 3 kDa molecular weight cutoff and digested separately overnight using trypsin chymotrypsin or Glu-C (Mass Spectrometry Grade, Promega) at a ratio of 1:30 (w/w) ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were then diluted to 6 M urea with 50 mM Tris-HCl pH 8 and Trypsin/Lys-C mix (Promega) was added to an enzyme:substrate ratio of 1:25 (w/w ...
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bioRxiv - Cell Biology 2022Quote: ... and dried in at 37 °C before overnight digestion with 30 μL trypsin (1:100 g/g, sequencing grade modified trypsin V5111, Promega) at 37 °C ...
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bioRxiv - Cell Biology 2019Quote: ... RT reaction was carried out in 15 ul reaction mixture at 40 °C for 60 min after addition of ImProm II (Promega). DNA fragments for L chain and the variable region of H chain (VH ...
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bioRxiv - Microbiology 2021Quote: ... The virus-serum mix was subsequently used to infect A549-hACE2-TMPRSS2 cells7 for 18 hours at 37 °C before adding ONE-Glo reagent (Promega) for measurement of the luciferase signal by relative luminescence units (RLUs) ...
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bioRxiv - Microbiology 2021Quote: ... primer TTC CGC AAG TTC ACC TAC C and the reverse (3’ – 5’) primer CGG GCC GGC CAT GCT TTA CG with GoTaq Flexi DNA polymerase (Promega) and the following cycling conditions ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were resolved in reducing and non-reducing SDS-PAGE gels and membranes were incubated overnight at 4°C with the following antibodies: rabbit polyclonal anti-human p75 intracellular domain (1:1000, Promega); mouse monoclonal anti-HA (1:2000 ...
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bioRxiv - Microbiology 2022Quote: All isolates were heat-extracted at 95°C for 20 minutes for polymerase chain reaction (PCR) using GoTaq (Promega M7132) and 8F and 1492R primers to amplify the whole 16S rRNA gene (4) ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were then diluted to 4 M urea with 50 mM Tris-HCl pH 8 and Trypsin/Lys-C mix (Promega) was added to an enzyme:substrate ratio of 1:250 (w/w ...
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bioRxiv - Systems Biology 2019Quote: ... and dried in an oven at 37 °C) before overnight digestion with 20 μL trypsin (1:100 g/g, sequencing grade modified trypsin V5111, Promega) at 37 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein precipitation was done using 6 volume of ice cold acetone and digested overnight at 37 o C using modified trypsin (Promega). Then peptides were fractionated by bRPLC and 8 fractions were collected ...
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bioRxiv - Genomics 2019Quote: To generate recombinant p53 we in vitro transcribed/translated human p53 with a c-terminal HA tag using a rabbit reticulocyte system (Promega). To generate fragmented genomic DNA we tagmented 50ng of human genomic DNA from MCF7 cells using the MuSeq kit (Thermo ...
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bioRxiv - Microbiology 2020Quote: ... One aliquot was stored at −80°C for later infectivity analyses and the other aliquot was treated with DNAse I (RQ1 DNAse, Promega) prior to purification of viral DNA using a PureLink Viral RNA/DNA minikit (ThermoFisher) ...
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bioRxiv - Microbiology 2020Quote: ... Protein digestion was performed using 1:100 (w/w) LysC (Wako Chemicals) for 4 h at 37°C and then finalised with 1:50 (w/w) trypsin (Promega) overnight at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... The virus-serum mix was subsequently used to infect A549-hACE2-TMPRSS2 cells [41] for 18 hours at 37°C before adding ONE-Glo reagent (Promega) for measurement of the luciferase signal by relative luminescence units (RLUs) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were incubated for 3 days at 35°C with 5% CO2 and cell viability was evaluated using the CellTiter Glo (Promega) post the 3-day incubation ...
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bioRxiv - Cancer Biology 2021Quote: The full length 3’ UTR of c-Myc was cloned into the pmirGLO Dual-Luciferase miRNA target expression vector (Promega). Luciferase activity was assayed in transfected cells using the Dual-Luciferase Reporter Assay (Promega) ...
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bioRxiv - Molecular Biology 2021Quote: The 1-450bp DNA region of the luciferase coding sequence was synthesized with G/C content optimized and ‘ATG’ codons removed and then inserted into the pGL3-Promoter vector (E1761, Promega) in place of the original full-length luciferase coding region using ClonExpress®II One Step Cloning Kit (C112-01 ...
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bioRxiv - Microbiology 2020Quote: ... protein lanes were cut into 10 pieces, destained (600 rpm, 37 °C, 200 mM NH4HCO3 in 30% acetonitrile) and digested with trypsin (sequencing grade, Promega) overnight at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... The reactions were subjected to primer extension at 42°C for 45 min using 1 unit of MMLV-RT (Promega) and 0.5mM of dNTPs ...
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bioRxiv - Microbiology 2020Quote: ... Reduced and alkylated proteins from band slices excised from the entire separation profile were in-gel digested at 37°C overnight with 100 ng trypsin (modified sequencing grade, Promega). Supernatants were dried in a vacuum centrifuge and resuspended in 0.1% TFA ...
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bioRxiv - Immunology 2019Quote: ... Samples were diluted 10-fold with 50 mM Triethylammonium bicarbonate buffer at pH 8 and incubated overnight at 37°C with sequencing grade trypsin (Promega) added at a 1:50 enzyme:substrate ratio (wt/wt).