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Citations for Promega :
1301 - 1350 of 2107 citations for 3 Ethyl 3 methyloxolane 2 5 dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... purified RNAs were additionally incubated with 2 units of RQ1-DNAse (Promega, cat# M6101) per 1 µg of RNA at 37°C for 30 min to eliminate plasmid DNA contamination ...
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bioRxiv - Biochemistry 2023Quote: ... or a combination of 2 μg of trypsin and 0.2 μg of LysC (Promega). Samples were incubated at 37 °C overnight ...
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bioRxiv - Microbiology 2024Quote: ... for 2 h at 25 °C and digested with trypsin (Promega, 37 °C, overnight) in TEAB buffer (50 mM ...
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bioRxiv - Cell Biology 2023Quote: ... and proteins were further digested by the addition of 2 μg trypsin (Promega #V5113) for 4 hours at room temperature on a shaker ...
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bioRxiv - Plant Biology 2024Quote: ... 2 mg of the extracted RNA underwent treatment with RQ1 RNase-Free DNAse (Promega) before being used for cDNA synthesis with SuperScript IV Reverse Transcriptase (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... peptides were collected as described in ProteaseMax application note (Promega, TB373, Rev. 2/15), and purified using Pierce C18 Spin Tips (Thermo Fisher PI84850) ...
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bioRxiv - Molecular Biology 2023Quote: ... and 24 hours later co-transfected with 500 ng psiCHECK™-2 Vector (Promega) containing the 3’UTR of human PLIN2 gene downstream to the Renilla luciferase gene ...
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bioRxiv - Cell Biology 2022Quote: ... with 2 uM Ponasterone A overnight and incubated with JF646 HaloTag ligand (Promega, GA1120) 15 minutes before imaging ...
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bioRxiv - Immunology 2022Quote: ... 1-2 µg RNA was reverse transcribed using AMV Reverse Transcriptase (Promega, Madison, WI). Primers were purchased from Integrated DNA Technologies (IDT ...
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bioRxiv - Plant Biology 2023Quote: ... Two (2) μg of total RNA were used for cDNA synthesis with MMLV (Promega) and random hexamers (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were digested for 18 h at 37 °C with 2 μg trypsin (Promega). After digestion ...
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bioRxiv - Cell Biology 2022Quote: ... and 1-2 µg of RNA was reverse transcribed into cDNA using GoScript (Promega).
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bioRxiv - Cancer Biology 2023Quote: ... 2 µg total RNA served as a template using M-MLV Reverse Transcriptase (Promega) and random hexamer primers following manufactureŕs protocol ...
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bioRxiv - Cell Biology 2024Quote: ... for 20ms with 2 pulses or lipid-mediated delivery using FuGENE HD (Promega; TM328) according to the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2024Quote: ... HaloTagged AP-2 σ2 was visualized by adding Janelia Fluor 646-HaloTag ligand (Promega). Ligand (100 nM ...
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bioRxiv - Biochemistry 2024Quote: ... the cells were transferred to CO2-independent media containing 2% GloSensor cAMP Reagent (Promega) and distributed into 384-well assay plates at a density of 4000 cells per well in 10 μL ...
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bioRxiv - Cell Biology 2024Quote: ... and 1-2 µg of RNA was reverse transcribed into cDNA using GoScript (Promega).
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bioRxiv - Molecular Biology 2020Quote: ... PCR amplification was performed in 25 μl reactions using 5 μl X Green GoTaq Flexi Buffer (Promega), 1.5 μl MgCl2 (25mM ...
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bioRxiv - Biophysics 2020Quote: ... samples from homozygous HaloTag-TEV mice were incubated overnight with 5 μM HaloTag Oregon Green ligand (Promega) in Phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2020Quote: ... alkylated with 5 mM iodoacetamide for 30 min in the dark and digested overnight with trypsin (Promega; 1 μg for cell-surface-enrichment samples ...
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bioRxiv - Microbiology 2022Quote: ... the modified region was amplified using primer SAM_Seq_Gen-23_FW (5’-GAT TTG AGG ACG ACT GGA CTG C) and SAM_Seq_Gen1116_RV (5’-GTC AAC TGA ACA ACC CCA AGG T) together with GoTaq polymerase (Promega), followed by cloning into pGEM T-easy vector system (Promega ...
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bioRxiv - Cancer Biology 2022Quote: ... and after 5 h the luciferase activity was measured using the Dual-Luciferase Reporter Assay System (Promega), according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... The embryos were then incubated in a 1:5,000 dilution of TMR HaloTag Ligand (5 mM; Promega) in Danieau’s medium ...
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bioRxiv - Microbiology 2020Quote: ... The reaction was stopped by the addition of 5.5μL of a mix containing 5% SDS and 0.25M EDTA and deproteinized with proteinase K (Promega) for 1hour at 37°C ...
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bioRxiv - Genetics 2020Quote: ... fragments of varying sizes (Supplemental Table 5) were cloned into the promoter-less pGL3-basic vector (Promega) using NheI and NcoI sites ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 5 μL (20x working stock of NanoBRET Tracer K5 [20 μM] in Tracer Dilution Buffer (Promega N291B)) was added to each well with the exception of the “no tracer” control wells ...
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bioRxiv - Plant Biology 2020Quote: ... 5 μg of RNA were depleted from contaminating DNA by treatment with RQ1 RNase-free DNase (Promega) and were reverse-transcribed with Superscript IV Reverse Transcriptase (Thermo Fisher Scientific) ...
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Psi promotes Drosophila wing growth through transcriptional repression of key developmental networksbioRxiv - Developmental Biology 2020Quote: ... 5 µL of RNA was used for each cDNA synthesis (GoScript™ Reverse Transcription System kit, Promega). qPCR was performed using Fast SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... and plates were incubated for 5 days before being assayed using the Cell Titer-Glo kit (Promega).
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bioRxiv - Biochemistry 2023Quote: A mixture of 10 ng of biotinylated BSA digest and 5 µg of K562 cell digest (Promega) was added to streptavidin beads (10 µL of suspension used ...
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bioRxiv - Microbiology 2023Quote: ... the modified region was amplified using primer SAM_Seq_Gen-23_FW (5’-GAT TTG AGG ACG ACT GGA CTG C) and SAM_Seq_Gen1116_RV (5’-GTC AAC TGA ACA ACC CCA AGG T) together with GoTaq polymerase (Promega), followed by cloning into pGEM T-easy vector system (Promega ...
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bioRxiv - Plant Biology 2022Quote: ... or with the same buffer supplemented with 5 U of calf intestine alkaline phosphatase (CIAP) from Promega. Samples were incubated at 30°C for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 ng of LHB promoter-containing reporter vector were transfected with 5 ng of pRLRenilla (#E2231, Promega), with or without 50 ng of pCMV6-NR2F2-WT or p.Arg246His and/or 5 ng of pCMX-NR5A1 ...
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bioRxiv - Genetics 2024Quote: ... then a 5 min final extension (72 °C)—was carried out with GoTaq Green Master Mix (Promega). Deletion loci from mutant flies were amplified using the genotyping primers above with four GoTaq Green PCR reactions per line ...
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bioRxiv - Developmental Biology 2023Quote: ... 16/32-cell embryos were co-injected with 5 pg of pRL-TK:renilla luciferase plasmid (Promega E2241) + 80 pg of the pGL4.23 luc2/miniP HumanPDX1 enhancer:firefly luciferase plasmid (wt or 6RFX motif mutant ...
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bioRxiv - Molecular Biology 2023Quote: The reaction mixture (10 μl) was prepared with 5 μl of rabbit reticulocyte lysate nuclease-treated (Promega), 2 μl of H2O ...
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bioRxiv - Cancer Biology 2022Quote: ... Following 5 days of drug incubation cell viability was assayed using 30μl of CellTiter-Glo® (Promega). Screens were performed in technical triplicate.
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bioRxiv - Physiology 2023Quote: ... Each 10 µl PCR reaction consisted of 5 µl of GoTaq® qPCR Master Mix (Promega Corporation), 0.5 µl of 10 µM forward primer ...
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bioRxiv - Microbiology 2024Quote: ... that included (for 1 well): 5 μl of the SYBR Green Real-Time PCR master mix (Promega), 1.25 μl of the forward and reverse primer (standardized KiCqStartTM primers ...
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bioRxiv - Plant Biology 2024Quote: ... 5 μg of RNA were depleted from contaminating DNA by treatment with RQ1 RNase-free DNase (Promega) and reverse-transcribed with Superscript IV Reverse Transcriptase (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... The secondary antibody (Goat anti-rabbit IgG HRP, Promega, diluted 1:133333 in 5% milk in PBS) was then applied for 50 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 µg of RNA was subjected to reverse transcription using M-MLV enzyme (Promega #M1705), dNTP mix 100 mM each (BLIRT #RP65 ...
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bioRxiv - Microbiology 2020Quote: ... Protein digestion was carried out by adding 2 μg of trypsin solution (Promega, Charbonnières, France) to the alkylated proteins ...
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bioRxiv - Bioengineering 2021Quote: ... and 1 and 2 μL of 50 ng/μL trypsin (in 100 mM TEABC, Promega) was added to the wells with single cells and two hundred carrier cells ...
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bioRxiv - Microbiology 2020Quote: ... Amplification was performed in 50 μl final volume using 2 U of GoTaq Polymerase (Promega) and 100 ng of DNA template in each reaction ...
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bioRxiv - Microbiology 2020Quote: ... Amplification was performed in 50 μl final volume using 2 U of GoTaq Polymerase (Promega) and 100 ng of Nichols DNA template ...
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bioRxiv - Cell Biology 2021Quote: ... and cloned into the XhoI and HindIII sites in the psiCHECK™-2 Vector (Promega) resulting in the plasmid pTal1-Luc ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 μg of total RNA was used for cDNA synthesis with AMV reverse Transcriptase (Promega) together with 20 pmol of oligonucleotides O135 (5’-TAGCGGCTGATGTTGAACTG-3’ ...
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bioRxiv - Immunology 2022Quote: ... transfected with ISD (21) at 2 µ g per dish using ViaFect (Promega, Madison, WI) at 3 µ L per 1 µ g of ISD based on manufacturer protocol ...
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bioRxiv - Immunology 2022Quote: ... transfection with ISD (21) at 2 µ g per dish using ViaFect (Promega, Madison, WI) at 3 µ L per 1 µ g of ISD based on manufacturer recommendations ...