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Citations for Promega :
1251 - 1300 of 2378 citations for 6 BROMO 3 4 DIHYDROQUINOLINE 2 1H THIONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Tryptic digestion was performed for 3 h with 0.1 μg trypsin at 37°C (Promega, V5113) per sample ...
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bioRxiv - Cell Biology 2023Quote: NRK cells were transfected with IP3R-3 siRNA (see below) using Transfast (Promega Corp.; Madison WI) using manufacturer’s instructions ...
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Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2024Quote: Genomic fragment containing the murine Rorc 3’UTR was cloned into psiCHECK2 luciferase reporter plasmid (Promega). This construct was also used to generate the let-7 ʻseed’ deletion mutant derivative using the QuikChange Multi Site Mutagenesis Kit (catalog 200514-5 ...
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bioRxiv - Plant Biology 2024Quote: RNAs including 5’ and 3’ UTRs were synthesized in vitro using T7 RNA polymerase kit (Promega). cDNA from A ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were pulsed for 4 h with CellTiter 96 Aqueous One solution reagent (Promega). Cell viability was measured by a luminometer ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... after which growth media was removed and 20 µl of 4% D-luciferin (Promega) in CO2 independent medium (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... overnight at 4°C and HRP-conjugated anti-rabbit antibody (1:2,000, W4011, Promega) for 1 hr at room temperature followed by ECL signal amplification and chemiluminescence detection (SuperSignal West Pico Chemiluminescent Substrate ...
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bioRxiv - Biochemistry 2022Quote: 4 × 105 HEK293T cells were lysed in 100 μl of Passive Lysis Buffer (Promega). 10 μl of cell lysates were separated on a NuPAGE 4-12% Bis-Tris polyacrylamide gel (ThermoFisher) ...
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bioRxiv - Microbiology 2020Quote: ... and then ligated for 16 h at 4 °C with T4 DNA ligase (Promega). After a further purification step ...
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bioRxiv - Biochemistry 2019Quote: 4 x 105 HEK293T cells were lysed in 100 μl Passive Lysis Buffer (Promega). 10 μl of cell lysates were boiled with 2.5 μl 4x gel loading buffer for 5 min at 98°C and run on a NuPAGE 4-12% Bis-Tris polyacrylamide gel for 45 min at 200 V ...
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bioRxiv - Molecular Biology 2020Quote: ... Chromosome 4: 101,716,750-101,718,250 forward strand) was cloned into pGL3-basic vector (E1751, Promega). Reporter vector ...
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bioRxiv - Microbiology 2024Quote: ... pH 8.0 before adding 4 µL of 0.5 µg/µL sequencing grade trypsin (Promega) and incubated over night at 37°C ...
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bioRxiv - Genetics 2024Quote: ... transfected with 4 µg of plasmid DNA using the FuGENE® HD Transfection (Promega) for 24 h ...
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bioRxiv - Systems Biology 2023Quote: ... for 4 h at 37 °C and then with trypsin (Promega, 1:100, w:w) overnight at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... synthesized from Gene Universal (USA) at 4°C overnight (T4 DNA ligase, Promega, USA). Cys-loop mutation ligation products were transformed into DH5α (ThermoScientific ...
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bioRxiv - Cell Biology 2020Quote: ... The AIO plasmid and the template plasmid were transfected into HAP1 STG-p62 (for mScarlet-AID-NBR1) or HAP1 WT (for GFP-AID-NBR1) cells using FuGene 6 transfection reagent (Promega, cat# e2691). 48h later the cells were sorted for mScarlet fluorescence in bulk ...
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bioRxiv - Microbiology 2020Quote: ... The env gene clones together with the env-deficient HIV-1 plasmid DNA (SG3Δenv) were contransfected into HEK293T cells (ATCC, Manassas, VA, USA) using the FuGene 6 transfection reagent (Promega, Madison, WI, USA). Two days after transfection ...
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bioRxiv - Biochemistry 2022Quote: ... (complete media) were co-transfected with our library of retinopathy variants and the Bxb1 recombinase expression vector using Fugene 6 (Promega, Madison, WI). Doxycycline (2 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... L981F. Pseudovirions were produced in HEK 293T/17 cells (ATCC cat. no. CRL-11268) by transfection using Fugene 6 (Promega, Catalog #E2692). Pseudovirions for 293T/ACE2 infection were produced by co-transfection with a lentiviral backbone (pCMV ΔR8.2 ...
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bioRxiv - Biochemistry 2020Quote: ... The mutation was confirmed by full-length Spike gene sequencing. Pseudovirions were produced in HEK 293T/17 cells (ATCC cat. no. CRL-11268) by transfection using Fugene 6 (Promega Cat#E2692) and a combination of Spike plasmid ...
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bioRxiv - Cancer Biology 2021Quote: ... Plasmids were packaged with the third-generation lentiviral packaging system (VSV-G, pMDLg, and pRSV-REV) and expressed in HEK293T cells via transient transfection with FuGENE 6 (Promega, Madison, WI). Virus-containing supernatants were harvested after 24 and 48 hours ...
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bioRxiv - Immunology 2021Quote: ... The mutation was confirmed by full-length spike gene sequencing. Pseudovirions were produced in HEK 293T/17 cells (ATCC cat. no. CRL-11268) by transfection using Fugene 6 (Promega, Catalog #E2692). Pseudovirions for 293T/ACE2 infection were produced by co-transfection with a lentiviral backbone (pCMV ΔR8.2 ...
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bioRxiv - Immunology 2021Quote: ... All mutations were confirmed by full-length Spike gene sequencing. Pseudovirions were produced in HEK 293T/17 cells (ATCC cat. no. CRL-11268) by transfection using Fugene 6 (Promega Cat#E2692) and a combination of Spike plasmid ...
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bioRxiv - Cancer Biology 2022Quote: ... 1000 and 5000 µM) or remained untreated for 6 days and viability was analyzed using the CellTiter-Glo Luminescent Cell Viability Assay (Promega, Dübendorf, Switzerland) to determine the adenosine triphosphate (ATP ...
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bioRxiv - Biochemistry 2022Quote: ... cells grown in 10 cm dishes in complete media were co-transfected with our library of cystic fibrosis variants and the Bxb1 recombinase expression vectors using Fugene 6 (Promega, Madison, WI). Doxycycline (2 μg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... of Dusp27 from EDL muscle DNA of C57BL/6 mice was inserted into a pGL4.10 promoter-less luciferase vector (Promega, Madison, WI, USA) digested with XhoI ...
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bioRxiv - Immunology 2024Quote: ... 6 μg of each vector was transfected into tumor cells plated on a 6-well plate using FuGENE6 transfection reagent (Promega, cat. E2691) and OPTI-MEM (GIBCO ...
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bioRxiv - Biochemistry 2023Quote: CFTR function measurements were carried out in recombinant stable cells were made from genetically modified HEK293T cells grown in 10 cm dishes in complete media as was previously described.37,38 Briefly these HEK293T cells contain a Tet-Bxb1-BFP “landing pad” where recombination results in the integration of a plasmid into the gDNA of the cell.37 These cells were co-transfected with plasmid and a Bxb1 recombinase expression vector using Fugene 6 (Promega, Madison, WI). Doxycycline (2µg/mL ...
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bioRxiv - Biochemistry 2024Quote: Total RNA was isolated from the cells of 6-well plates treated as above using the Maxwell RSC simplyRNA kit and the Maxwell RSC Instrument (both Promega, Southampton, UK) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... or pB.2-eIF2α-S51A plasmids by use of FuGENE6 (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Each reaction was treated with 2 units of RQ1 DNase (Promega) for 30 min at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... 2 μl of Trypsin/Lys-C mix (0.5μg/μl, Promega, V5073) was added to each sample and incubated for 3 hrs at RT in the dark ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were digested by addition of 2 % (w/w) trypsin (Promega) over night at 37°C after adding 50 mM ammonium hydrogen carbonate to a final concentration of 1 M urea ...
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bioRxiv - Plant Biology 2020Quote: ... from 2 μg RNA treated with RQ1 RNase-free DNase (Promega). The cDNA was then diluted 20 times in water and 5 μl of the dilution was used for the realtime qPCR analysis ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were additionally diluted 2-fold and digested with trypsin (Promega, Sequencing Grade Modified Trypsin ...
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bioRxiv - Cell Biology 2022Quote: ... 2 µg Lys-C/Trypsin mix protease (Promega, Mass spec grade) were added to each sample (10 mM EPPS pH 8.5 ...
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bioRxiv - Cancer Biology 2019Quote: ... The RdRP products were treated with RNase I (2 U, Promega) at 37°C for 2 hours to digest single-stranded RNAs completely ...
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bioRxiv - Microbiology 2019Quote: ... 5 μl of 2× GoTaq q-PCR master mix (Promega, USA) and 0.6 μM of AF77/78 or AF79/80 primer pairs targeting a region close to the Cori or to the terminus (ter) ...
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bioRxiv - Genetics 2020Quote: ... 2 μg RNA were treated with RQ1 RNase-free DNase (Promega) followed by reverse transcription using M-MLV Reverse Transcriptase (Promega ...
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bioRxiv - Developmental Biology 2022Quote: ... 2 μg of MS grade Trypsin Platinum (Promega, Madison, WI, USA) in 40 μL of digestion buffer containing 50 mM TEAB was added into the filter and digested at 37°C for 16 h ...
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bioRxiv - Cancer Biology 2022Quote: ... Nano-Glo Luciferase Assay Substrate furimazine (2 µl ml-1, Promega) was added and the nLuc released upon proteolytic cleavage of the receptor was measured on a plate reader Mithras LB 940 (Berthold Technologies ...
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bioRxiv - Physiology 2023Quote: ... The assay medium was supplemented with 2% GloSensor Reagent (Promega #E1291) and 0.1% BSA ...
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bioRxiv - Physiology 2024Quote: The 2-DG glucose uptake for tissues was analyzed by Promega Glucose uptake-Glo assay method (#J1341) ...
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bioRxiv - Molecular Biology 2024Quote: ... Crosslinks were reversed by addition of 2 uL Proteinase K (Promega) and incubation at 65 °C for 12-16 hours in a Thermomixer ...
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bioRxiv - Molecular Biology 2024Quote: ... 10X Primer extension buffer and 2 U AMV Reverse Transcriptase (Promega) were added to the annealing mixture and incubated for 1 hour at 42 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 ng of Renilla luciferase expressing pRL SV40 plasmid (E2231, Promega), as internal control ...
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bioRxiv - Microbiology 2023Quote: ... 5 μM 11S and 2 μl furimazine solution (Promega Cat. # N1610) were added and the luminescence read at 1 min intervals for 10 min using the FLUOstar Omega using a gain value of 3,000 ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids (2 μg /dish) were transfected using FuGENE6 transfection reagent (Promega), according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2023Quote: ... Any remaining RNA was removed with 2 µl RNase A (Promega) added to a pool of DNA samples ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2 μL/well of One-Glo detection reagent (Promega, cat # E6120) was added to all microplate wells via Multidrop ...