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Citations for Promega :
1201 - 1250 of 3017 citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Promega) and 6 µM ATP (UltraPure, Promega) in the kinase reaction buffer (40 mM Tris-HCl pH 7.5 ...
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bioRxiv - Genetics 2020Quote: ... by transient transfection with FuGENE 6 (Promega). On day 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... FuGENE 6 transfection reagent (Promega, USA #E2691) diluted in serum-free DMEM was added to the plasmid mixture at 1.5:1 (reagent ...
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bioRxiv - Immunology 2023Quote: ... and lentivectors using Fugene 6 (Promega, E2691). 24 hours after transfection ...
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bioRxiv - Biochemistry 2024Quote: ... Transfections were performed with Fugene 6 (Promega) per manufacturer’s directions for 24-48 hours before selection with 400 μg/mL hygromycin B.
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bioRxiv - Biophysics 2023Quote: ... and FuGENE 6 transfection reagent (Promega, USA), propagated ...
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bioRxiv - Neuroscience 2023Quote: ... using Fugene 6 Transfection Reagent kit (Promega) according to manufacturers’ instructions ...
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bioRxiv - Biochemistry 2022Quote: ... and transiently transfected with Fugene 6 (Promega), following the manufacturer’s instructions ...
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HIVtat Alters Epithelial Differentiation State and Increases HPV16 Infectivity in Oral KeratinocytesbioRxiv - Microbiology 2023Quote: ... Cells were transfected with Fugene-6 (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Cells were transfected with Fugene-6 (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The Fugene 6 reagent (Promega, Madison, USA) was used at a 3:1 rate ...
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bioRxiv - Cell Biology 2024Quote: ... 1ug of plasmid and FuGene 6 (Promega) were used ...
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bioRxiv - Neuroscience 2024Quote: ... cells were incubated with Fugene 6 (Promega) and plasmids for 12 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... using Fugene 6 transfection reagent (Promega, E2691), following manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2024Quote: Fugene 6 was obtained from Promega (E2691). Lipofectamine 2000 was obtained from Thermo-Fisher Scientific (11668019) ...
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bioRxiv - Molecular Biology 2024Quote: ... Transfection was performed using Fugene 6 (Promega). Transfected HeLa cells were washed with Dulbecco’s phosphate-buffered saline (PBS) ...
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bioRxiv - Cell Biology 2024Quote: ... FuGENE 6 Transfection reagent (Promega, cat. #E2691), phosphate buffered saline (Wisent ...
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bioRxiv - Bioengineering 2023Quote: ... Reverse transcription was performed at 70 °C for 5 min and 42 °C for 60 min with 1 µg total RNA using ImProm-II reverse transcriptase (Promega, Madison, WI, USA) and 250 ng oligo(dT)12-18 primers (ThermoFisher ...
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bioRxiv - Microbiology 2021Quote: ... The kinase activity assay was performed with 4 µl beads in a 384-well plate using the ADP-Glo™ Kinase Assay (Promega, Madison, WI). The luminescence of each well was read using a Synergy™ H1 Hybrid Multi-Mode Microplate Reader (BioTek Instruments ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... PHH were infected with HepAD38-derived GTD virus at 500 viral genome equivalents per cell in maintenance medium supplemented with 4 % PEG 8000 (Promega, Madison, WI; V3011). For patient sera infections ...
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bioRxiv - Microbiology 2023Quote: ... were not screened for Wolbachia.All PCRs were performed in a final reaction volume of 20 μl including 4 μl of 5X reaction buffer (Promega, Madison, WI, USA), 1.5 μl of dNTPs (2.5 mM) ...
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bioRxiv - Microbiology 2022Quote: ... then dipped in a well of a 96-well PCR plate filled with 20 µl of a gyrB PCR mix (4 µl of 5x GoTaq buffer (Promega, Madison, WI, USA), 0.4 µl of 10mM dNTP ...
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bioRxiv - Biochemistry 2023Quote: ... and 20 µL of 1:1000 (diluted in phenyl red-free DMEM with 4% FBS) HaloTag™ NanoBRET™ 618 Ligand (Promega G9801) was added to each well ...
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bioRxiv - Plant Biology 2022Quote: ... as primary antibodies and anti-mousse IgG-HRP conjugated secondaries antibodies (Mouse IgG, HRP conjugate W402B, Promega; 1/5000 in TBST, 4 hours). As a loading control we used anti-tubulinα antibodies as primary antibodies (1/1000 in 5 % milk over-night) ...
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bioRxiv - Genetics 2024Quote: ... DNA was isolated from a variety of tissues that were collected from 4- and 8-month-old male mice using a Maxwell® 16 Mouse Tail DNA Purification kit (Promega, Madison, WI) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... Huh-7 cells were subsequently lysed with 50 μL lysis reagent (Promega), and 30 μL lysates were transferred to 96-well Costar flat-bottom luminometer plates (Corning Costar ...
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bioRxiv - Microbiology 2021Quote: ... We synthesized cDNA from 7 μl of RNA using m-MLV (Promega) with random hexamers for PCRs using Culex primers and ...
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bioRxiv - Cell Biology 2022Quote: ... The sample was digested with 7 μl 0.1 μg/μl trypsin (Promega) /AB over night at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... Huh-7 cells were subsequently lysing with 50 μL lysis reagent (Promega), and 30 μL of the lysates were transferred to 96-well Costar flat-bottom luminometer plates (Corning Costar ...
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bioRxiv - Cancer Biology 2023Quote: ... Viable cellularity was quantified at day 7 with CellTiter-Glo reagent (Promega) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: LC-MS/MS-based mono-methylated/dimethylated-peptide analyses: NADK purified from HEK293T cells was digested with Glc-C and Lys-C mixture (sequencing grade, Promega, protein: Glc-C: Lys-C = 20:1:1) at 37°C for 16 h and desalted using C18 Zip-Tips (Millipore).
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bioRxiv - Neuroscience 2022Quote: ... and digested overnight at 37 °C with Trypsin/Lys-C (Promega, V5071) at a ratio of 1/50 ...
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bioRxiv - Biochemistry 2023Quote: ... overnight at 37°C or sequentially with trypsin and Glu-C (Promega) at an enzyme-to-substrate ratio of 1:20 and 1:50 at 37°C and 25°C ...
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bioRxiv - Plant Biology 2023Quote: ... The PHN-SpCas9-4×BsaI was then digested with BsaI and purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA). The annealed oligos FaPDSF1/R1 and FaPDSF2/R2 were mixed and ligated to the purified pHN-SpCas9-4×BsaI to form the final pHN-SpCAS9-FaPDSsgRNAs construct.
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bioRxiv - Cell Biology 2020Quote: ... or Lys-C (Promega). Proteins were resuspended in 100 µl of the appropriate digestion buffer (trypsin ...
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bioRxiv - Developmental Biology 2020Quote: ... Endoproteinase Lys-C (Promega), 5 μL at 1 μg/μL ...
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Multi cell line analysis of lysosomal proteomes reveals unique features and novel lysosomal proteinsbioRxiv - Molecular Biology 2020Quote: ... rLys-C (Promega, V1671) added at an enzyme to protein ratio of 1 to 100 (w/w) ...
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bioRxiv - Biochemistry 2021Quote: ... or Lys-C (Promega) at 37 °C for 12 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Trypsin/Lys-C (Promega) solution was added and incubated at 37 °C for 12 h ...
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bioRxiv - Microbiology 2020Quote: ... and Glu-C (Promega). Reaction mixtures were dried and peptides/glycopeptides were extracted using C18 Zip-tip (MerckMilipore ...
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bioRxiv - Biochemistry 2022Quote: ... or Glu-C (Promega); aliquots of CD16a proteins were digested respectively using chymotrypsin (Athens Research and Technology) ...
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bioRxiv - Biochemistry 2022Quote: ... or Glu-C (Promega); aliquots of CD16a proteins were digested respectively using chymotrypsin (Athens Research and Technology) ...
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bioRxiv - Biochemistry 2022Quote: ... and Glu-C (Promega); aliquots of S proteins were digested respectively using alpha lytic protease (New England BioLabs) ...
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bioRxiv - Molecular Biology 2023Quote: ... or Glu-C (Promega). Next day ...
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bioRxiv - Biochemistry 2023Quote: ... trypsin/Lys-C (Promega) and 0.1% (w/v ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were seeded in a 6-well plate and transfected with 750 ng of each of the AT1R-TANGO plasmids using FUGENE 6 Transfection (PROMEGA). The following day ...
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bioRxiv - Immunology 2020Quote: ... Plasmid transfections of IFITM1 3’ UTR reporter plasmids (500 ng per single well of a 6-well plate) were performed using the FuGENE 6 (Promega), according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... 1.5 μg of total DNA (1:10 ratio of the Cre plasmid to the fusion protein plasmid) were mixed with 6 μl of Fugene 6 transfection reagent (Promega) in 100 μl of Opti-MEM ...
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bioRxiv - Cell Biology 2023Quote: ... cells were plated on three 10 cm tissue culture dishes per condition and transfected 24 h before lysis using FuGENE 6 (6-12 μg total plasmid DNA; Promega). Published protocol can be found on Protocols.io (dx.doi.org/10.17504/protocols.io.kxygx3zeog8j/v1).
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bioRxiv - Genetics 2023Quote: ... and used for thermal cycling (35× 30sec at 95°C, 55°C and 72°C) with 1X GoTaq G2 Master Mixes (Promega, cat.no.M7823), 0.2µM CTNS and 0.2µM bactin1 primers (Supplementary Table S2) ...