Labshake search
Citations for Promega :
1451 - 1500 of 3017 citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... 5 ng pRL-SV40 (Promega), and 375 ng total amount of testing plasmids individually or in combinations ...
-
bioRxiv - Molecular Biology 2024Quote: ... 5 µl H2O (Promega, P1199). Samples were incubated 1 hour at 56°C followed by 16 to 24 hours at 80°C and a holding step at 4°C ...
-
bioRxiv - Molecular Biology 2024Quote: ... 5 µg/mL Trypsin (Promega), 2 M urea ...
-
bioRxiv - Bioengineering 2022Quote: ... Proteins were digested using Lys-C (Wako, 1:40, w/w, overnight under gentle shaking at 30°C) and modified trypsin (Promega, 1:60 ...
-
bioRxiv - Cell Biology 2022Quote: ... two micrograms of RNA from each sample were reverse transcribed at 42 °C for 1 h followed by 95 °C for 10 min M-MLV Reverse Transcriptase (Promega) and oligo(T)18 primers (Takara) ...
-
bioRxiv - Cancer Biology 2021Quote: ... The proteins were diluted 1:5 with 100 mM ammonium bicarbonate and digested overnight at 37°C following the addition of 1 µg of Trypsin/Lys-C protease mixture (Promega) and calcium chloride to a concentration of 1 mM ...
-
bioRxiv - Systems Biology 2020Quote: ... pH 8.0 and digested for 16 hours on a shaker at 37 °C with a 1:40 ratio of Trypsin/Lys-C mix (Promega). Each sample was de-salted using HLB plates (Oasis HLB 30μm ...
-
bioRxiv - Genomics 2022Quote: ... samples were diluted 3 times with 50mM Tris buffer (down to 2M final urea concentration) and digested overnight at 37°C using a Trypsin/Lys-C mixture (Promega) at a ratio 1:100 w/w ...
-
bioRxiv - Microbiology 2020Quote: ... All samples were digested overnight at 37 °C using a Trypsin/Lys-C Mix (mass spec grade, Promega, Madison, WI) (enzyme/substrate of 1:100 w/w ...
-
bioRxiv - Cell Biology 2021Quote: ... Eluted proteins from control HeLa cells with C-terminal mEGFP tag were digested with mass-spectrometry grade Lys-C and trypsin (Promega). Eluted proteins from a cell line lacking full-length Cdc20 (CDC20_M1-fs-M43 ...
-
bioRxiv - Cell Biology 2021Quote: ... 50°C for 60 min and 70°C for 15 min) in the presence of 10 fg of Luciferase RNA (Promega) using the SuperSript III reverse transcription kit (Invitrogen) ...
-
bioRxiv - Microbiology 2023Quote: ... Mtb samples were inactivated at >95°C for 20 min and stored at -20°C until analyzing the ATP levels using the BacTiter Glo assay (Promega) as previously described (19) ...
-
bioRxiv - Cancer Biology 2023Quote: ... 58 °C for 45 seconds and 72 °C for 1 minute (32 cycles) and 72 °C for 10 minutes (1 cycle) using GoTaq G2 DNA polymerase (Promega). Additionally ...
-
bioRxiv - Neuroscience 2024Quote: ... the bead and protein mixtures were washed twice with 80% ethyl alcohol and once with 100% ACN before being resuspended in 95 µL NH4HCO3 prior to overnight on-bead digestion (enzyme–protein ratio of 1:20) at 1 000 rpm at 37 °C using modified porcine trypsin/Lys-C Mix (Mass Spectrometry Grade, Promega). The digestion was stopped using trifluoroacetic acid (TFA ...
-
bioRxiv - Developmental Biology 2023Quote: ... The modified proteins were digested with lysyl endopeptidase (Lys-C) (Wako) for 3h at 37° C and subsequently digested with trypsin (Promega) overnight at 37° C ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... in which proteins were reduced (DTT) and alkylated (IAA) before the digestion with Trypsin and Lys-C (Trypsin/Lys-C Mix, Mass Spec Grade Promega) for 14 hours at 37° C ...
-
bioRxiv - Cell Biology 2024Quote: ... with the following modification: Digestion was performed at 37°C overnight using 400 ng of Trypsin/Lys-C mix (Promega) in a buffer of 50 mM ammonium bicarbonate with 0.01% ProteaseMax surfactant added (Promega) ...
-
bioRxiv - Cell Biology 2024Quote: ... pH 8.5 to a final urea concentration of 2 M for Trypsin/Lys-C based overnight protein digestion at 37 °C (0.5 µg protease, Mass Spectrometry grade, Promega V5072). Peptide Purification and Labeling ...
-
bioRxiv - Neuroscience 2020Quote: ... RT-qPCR was performed on the ViiA 7 Real-Time PCR System using GoTaq qPCR master mix (Promega) according to the manufacturer’s protocols ...
-
bioRxiv - Cancer Biology 2021Quote: ... ATP levels at day 0 and day 7 were measured using CellTiter-Glo Luminescent Cell Viability Assay (Promega). For analysis ...
-
bioRxiv - Microbiology 2024Quote: ... The cells were then lysed 7 h later in 20 µl of Renilla luciferase assay lysis buffer (Promega), and luciferase activity was quantified using a Tristar LB 941 luminometer (Berthold Technologies ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cell viability was assessed 7 days post-treatment using the CellTiter-Glo® 3D Cell Viability assay (Promega).
-
bioRxiv - Cancer Biology 2024Quote: ... Tumor volumes were assessed 1-2 times per week by mixing 7 µL tail vein blood with 7 µL 0.5 mM EDTA (pH 8.0) and quantifying luminescence using Promega GloMax Plate Reader (Promega) and the substrate coelenterazine (NanoLight Technology ...
-
bioRxiv - Biochemistry 2020Quote: ... and then digested with trypsin/Lys-C (Promega) for 16 h at 37°C ...
-
bioRxiv - Cell Biology 2020Quote: ... alkylated and digested with endoproteinase Lys-C (Promega) followed by modified trypsin (Promega ...
-
bioRxiv - Microbiology 2022Quote: ... 400 ng/mL Trypsin/Lys-C (Promega, USA) were added on spin filter and incubated overnight at 37 °C ...
-
bioRxiv - Biochemistry 2022Quote: ... 0.5 µg of Lys-C / Trypsin mix (Promega) was added ...
-
bioRxiv - Immunology 2020Quote: MS was performed on Trypsin/Lys-C (Promega), and Chymotrypsin (Promega ...
-
bioRxiv - Biochemistry 2023Quote: ... 200 ng of Trypsin/Lys-C mix (Promega) was added ...
-
bioRxiv - Biochemistry 2022Quote: ... a combination of trypsin and Glu-C (Promega), or a combination of trypsin and AspN (Promega) ...
-
bioRxiv - Biochemistry 2022Quote: ... a combination of trypsin and Glu-C (Promega), or a combination of Glu-C and AspN (Promega) ...
-
bioRxiv - Biochemistry 2022Quote: ... a combination of trypsin and Glu-C (Promega), or AspN (Promega) ...
-
bioRxiv - Microbiology 2022Quote: ... Trypsin/Lys-C mix (Mass Spec Grade, Promega) was added to protein solution at 1:50 ratio (trypsin ...
-
bioRxiv - Molecular Biology 2023Quote: ... C-terminal large bit (LgBiT) tagged construct (Promega) which is transcribed under control of the HSV-TK promoter.
-
bioRxiv - Biochemistry 2023Quote: ... and 1 μg of trypsin/Lys-C (Promega) on a rotisserie for 18 h at ambient temperature ...
-
bioRxiv - Biochemistry 2024Quote: ... Mass spectrometry-grade Trypsin/Lys-C mix (Promega) was used for digestion with an overnight incubation at 37°C ...
-
bioRxiv - Cell Biology 2024Quote: ... 0.2 µg of Trypsin/Lys C (V5071, Promega) was added into each 1001µl sample in 501mM ammonium bicarbonate overnight at 371°C ...
-
bioRxiv - Biophysics 2024Quote: ... and a C-terminal HaloTag gene (Promega, G8031) followed by a 6×His-tag sequence ...
-
bioRxiv - Molecular Biology 2024Quote: ... 10 μL of Trypsin/Lys-C Mix (Promega) dissolved in 100 uL 25 mM AmBic in LC-MS grade water and incubated at 37 °C overnight with a top up of 10 μL trypsin after 2 hours ...
-
bioRxiv - Biochemistry 2022Quote: 2’-Deoxythymidine-5’-triphosphate (dTTP) and 2’-deoxyguanosine-5’-triphosphate (dGTP) were obtained from Promega. 2-14C labeled 2’-deoxythymidine-5’-triphosphate (2-14C-dTTP ...
-
bioRxiv - Cell Biology 2020Quote: ... in pbs containing 5 mM glucose and supplemented with 5 µM of Nanoglo (Promega #N1120) or coelenterazine-H (Dalton #50909-86-9) ...
-
bioRxiv - Genomics 2024Quote: ... and MgCl2 (final 5 mM) and 5 units of RQ1 RNase-free DNase I (Promega) were added ...
-
bioRxiv - Neuroscience 2023Quote: ... was performed by incubating the proteins first with endoproteinase Lys-C (Wako, #125-05061,)) in 8 M urea for 4h at 37°C followed by an overnight trypsin digestion (Promega #V511A,) in 2 M urea at 37°C ...
-
bioRxiv - Cell Biology 2023Quote: ... the 200k pellets were digested for 2 h at 37°C with 0.4 µg of Trypsin/Lys-C (Promega CAT#: V5071) and then overnight by adding 0.4 µg of Trypsin/Lys-C ...
-
bioRxiv - Physiology 2024Quote: ... further steps were performed according to the manufacturer’s protocol using proteases trypsin and Lys-C (2 h at 47° C, 1:15 and 1:30, respectively, Promega, USA). Peptides (20 μg ...
-
bioRxiv - Physiology 2024Quote: ... and hydrolyzed into the S-Trap column using trypsin and Lys-C (2 h at 47 °C, 1:15 and 1:30, respectively, all Promega, USA) in 40 μl 50 mM triethylammonium bicarbonate according to the manufacturer’s recommendations ...
-
bioRxiv - Cell Biology 2020Quote: ... Plasmid DNA and siRNA transfections were performed using FuGENE 6 Transfection Reagent (Promega) and Lipofectamine RNAiMAX (Invitrogen) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were transfected with either Cmas or Slc35a1 DNA using FuGene 6 (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... They were then transfected with pEYFP-XRCC1 (1µg) and 6µl Fugene 6 (Promega) in Optimem (Gibco ...
-
bioRxiv - Cancer Biology 2021Quote: ... FuGENE 6 transfection reagent and CellTiter-Glo assay system were obtained from Promega. SMARTpool siGENOME siRNA targeting NTC ...