Labshake search
Citations for Promega :
1051 - 1100 of 1643 citations for Recombinant Rabbit CD274 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... Protein pellets were resuspended in the digestion buffer as previously described and trypsin (Promega, UK) digested to peptides74 ...
-
bioRxiv - Genetics 2022Quote: Proteins were produced in vitro via the TNT SP6 Quick Coupled Transcription/Translation System (Promega). If two proteins were to be tested together in a single binding reaction ...
-
bioRxiv - Biochemistry 2023Quote: Proteins labeled with [35S]-methionine were produced by coupled transcription and translation reactions (T7, Promega). Translation products were partially purified by ammonium sulfate precipitation [87] ...
-
bioRxiv - Biochemistry 2023Quote: ... coli and HeLa cells as well as of a commercially available yeast protein digest (Promega), The ratios of the peptide amount of each species within the three mixtures are given in Table S1.
-
bioRxiv - Neuroscience 2023Quote: ... Proteins were digested overnight at 37° C with 1 μg mass spectrometry grade Trypsin (Promega). The resulting peptide sample was acidified with 10% formic acid and 10% trifluoroacetic acid (TFA ...
-
bioRxiv - Plant Biology 2023Quote: Proteins were expressed using the TnT Coupled Wheat Germ Extract System with SP6 promoter (Promega). Protein expression was confirmed by Western Blot ...
-
bioRxiv - Cell Biology 2023Quote: ... The proteins were resuspended in 100 mM NH4HCO3 and were digested with trypsin (Promega, UK) at an enzyme-to-substrate ratio of 1:25 ...
-
Arabidopsis actin-binding protein WLIM2A links PAMP-triggered immunity and cytoskeletal organizationbioRxiv - Plant Biology 2023Quote: ... Proteins were then digested overnight at 37°C with trypsin (Porcine trypsin, Promega, Fitchburg, WI). After stopping the digestion with 1% formic acid ...
-
bioRxiv - Cell Biology 2023Quote: ... Test proteins were generated in vitro using the TNR quick-coupled transcription-translation kit (Promega). For radiolabeling ...
-
bioRxiv - Neuroscience 2023Quote: ... proteins on beads were digested with the addition of 2 µg trypsin (Promega, Cat# V511A) for 18 h at 37°C.
-
bioRxiv - Molecular Biology 2023Quote: ... we labeled HALO-tagged49 proteins with HALO-Alexa488 or HALO-TMR dyes (Promega, G1001, G8251) by incubating the concentrated protein with dye for 1 hour on ice ...
-
bioRxiv - Cell Biology 2024Quote: ... Proteins on the beads were digested by adding 200 ng trypsin/Lys-C mix (Promega) at 37 °C overnight ...
-
bioRxiv - Biochemistry 2024Quote: ... For protein synthesis in reticulocyte lysate the TNT® Quick Coupled Transcription/Translation System (Promega) was used and performed according to the manufacture’s protocol employing 1 mM unlabeled methionine ...
-
bioRxiv - Cell Biology 2024Quote: ... cells transfected with HALO-fusion protein were supplemented with 100 nM TMR HALO ligand (Promega). Cells were fixed at RT for 20 min in 3.7% formaldehyde (FA ...
-
bioRxiv - Molecular Biology 2024Quote: ... Proteins were digested by adding 5 μL of sequencing grade trypsin (0.1 mg/mL) (Promega) for three h at 37 °C ...
-
bioRxiv - Microbiology 2024Quote: ... Proteins were then digested using 250 ng of trypsin enzyme (Sequencing Grade Modified Trypsin, Promega) and incubation for 1h at 37°C ...
-
bioRxiv - Developmental Biology 2021Quote: ... and the resulting mRNAs (2 μg) were translated in 50 μl of rabbit reticulocyte lysate (Promega). Flag-tagged proteins were purified by immunoprecipitation with anti-Flag M2 antibody (Sigma ...
-
bioRxiv - Molecular Biology 2020Quote: ... followed by a secondary anti-rabbit IgG antibody conjugated to alkaline-phosphatase (Promega, Madison, WI, USA). The membranes were blocked in TBST (150 mM NaCl ...
-
bioRxiv - Molecular Biology 2020Quote: ... at a 1:1000 dilution and a secondary antibody against rabbit coupled to HRP (Promega W4011) at a 1:20 000 dilution.
-
bioRxiv - Cell Biology 2020Quote: ... Horseradish peroxidase (HRP)-coupled secondary antibodies directed against mouse or rabbit IgG were purchased from Promega. Chemiluminescence images were acquired using the ImageQuant LAS 500 system (GE Healthcare).
-
bioRxiv - Molecular Biology 2020Quote: Constructs for expression in rabbit reticulocyte lysate (RRL) were based on the SP64 vector (Promega, USA). Constructs for translation in the PURE system were generated from the T7 PURExpress plasmid (New England Biolabs ...
-
bioRxiv - Bioengineering 2020Quote: ... and detected with anti-rabbit or anti-human HRP-conjugated secondary antibodies (Promega W4011 and W4031). Blots were developed using ECL (ThermoScientific 32106 ...
-
bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies used were: rabbit and mouse anti-βGal (1/1000; Promega and MP Biomedicals, respectiveley), rat anti-Ser (1/1000 ...
-
bioRxiv - Cancer Biology 2020Quote: ... and then with 1:5000 of anti-rabbit (#W401B) or anti-mouse (#W402B) secondary antibodies (Promega).
-
bioRxiv - Genetics 2019Quote: ... As secondary antibodies horseradish peroxidase (HRP)-conjugated α-mouse or α-rabbit IgG (Promega, 1:4000) were used ...
-
bioRxiv - Cell Biology 2022Quote: ... Subsequently anti-rabbit IgG horseradish peroxidase (HRP)-conjugated secondary antibody (Cat. No. W4011, Promega, Madison, WI) was used to detect the protein of interest with chemiluminescence reaction ...
-
bioRxiv - Cell Biology 2022Quote: ... washed and incubated with secondary antibody goat-anti-rabbit-HRP or goat-anti- mouse-HRP (Promega) for 1 h ...
-
bioRxiv - Cell Biology 2022Quote: Constructs for expression in rabbit reticulocyte lysate (RRL) used the SP64 vector as a backbone (Promega). For in vitro insertion reactions ...
-
bioRxiv - Genomics 2022Quote: ... Sections were then incubated with a horseradish peroxidase-labeled anti-rabbit antibody (1:300, Promega, W401B) for 1 hour at room temperature ...
-
bioRxiv - Immunology 2022Quote: ... mixed with Lumit BiT Set 2 (anti-mouse Ab-SmBiT, anti-rabbit Ab-LgBiT, Promega W1220) and added for 90min ...
-
bioRxiv - Plant Biology 2022Quote: ... for 2 h as primary antibody and peroxidase-conjugated goat anti-rabbit antibody (1:10,000; Promega) for 1 h with 5 times 10 min washes in-between the incubations ...
-
bioRxiv - Neuroscience 2022Quote: ... supernatant and pellet fractions were spotted onto nitrocellulose and blotted with rabbit anti-HaloTag pAB (Promega). For microscopic examination ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... Horseradish peroxidase (HRP)-coupled secondary antibodies directed against mouse or rabbit IgG were purchased from Promega for chemiluminescence imaging ...
-
bioRxiv - Biochemistry 2022Quote: ... The mRNA obtained was used for in vitro translation in Flexi Rabbit Reticulocyte Lysate System (Promega) and the resulting lysate was directly used in the import reaction ...
-
bioRxiv - Biochemistry 2023Quote: ... Horseradish peroxidase (HRP)-coupled secondary antibodies directed against mouse or rabbit IgG were purchased from Promega. Chemiluminescence images were acquired using the ImageQuant LAS 500 or 600 system (GE Healthcare).
-
bioRxiv - Plant Biology 2023Quote: ... bound antibodies were revealed with a peroxidase-linked secondary anti-rabbit antibody (Promega, Madison, WI, USA) and visualized by chemiluminescence (ImmunoStar Zeta ...
-
bioRxiv - Plant Biology 2024Quote: ... the membrane was incubated with a horseradish peroxidase (HPR) conjugated anti-rabbit antibody (Promega, Walldorf, Germany) diluted 1:10.000 in PBS-T with 3% milk powder for 1 h ...
-
bioRxiv - Microbiology 2020Quote: ... Reduced and alkylated proteins were in-gel digested with 100 ng trypsin (modified sequencing grade, Promega) overnight at 37°C ...
-
bioRxiv - Cell Biology 2020Quote: ... GST fusion proteins were isolated by binding to glutathione magnetic beads (MagneGST™ Glutathione Particles, Promega) for 30 min at room temperature on a rotating wheel ...
-
bioRxiv - Plant Biology 2020Quote: ... 3×HA-fused CabLCV MP and NSP proteins were independently expressed using the TNT system (Promega) according to the manufacturer’s recommendations ...
-
bioRxiv - Molecular Biology 2020Quote: ... Proteins were digested with Lys-C (Wako Chemicals) for 6 hours and trypsin (Trypsin Gold, Promega) overnight.
-
bioRxiv - Cell Biology 2022Quote: ... Pellets were lysed and bait proteins in the lysates were bound to HaloLink Magnetic Beads (Promega) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... and proteins were subjected to overnight digestion with mass spec grade Trypsin/Lys-C mix (Promega). Following digestion ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with plasmids encoding fluorescently tagged proteins using FuGENE® HD Transfection Reagent (Promega), following manufacturer’s protocol and assayed 1-3 days after transfection ...
-
bioRxiv - Systems Biology 2019Quote: ... Enzymatic protein hydrolysis was initiated by the addition of trypsin (Sequencing Grade Modified Trypsin, Frozen, Promega) at a ratio of 1:50 ...
-
bioRxiv - Microbiology 2019Quote: ... proteins were incubated overnight at 37 °C with 500bng trypsin (Trypsin Gold Mass Spectrometry Grade, Promega). Peptides were recovered by centrifugation.
-
bioRxiv - Cell Biology 2019Quote: ... Proteins were digested in-gel with trypsin (0.17 μg in 10 μl 40 mM NH4HCO3, Promega) at 37°C over night ...
-
bioRxiv - Molecular Biology 2019Quote: ... Activity was normalized to total protein following quantification using the Dual Luciferase Reporter Assay System (Promega).
-
bioRxiv - Biochemistry 2020Quote: ... proteins were acetone precipitated and digested using LysC (Wako sequencing grade) and trypsin (Promega sequencing grade), as described in (Buczak et al. ...
-
bioRxiv - Molecular Biology 2021Quote: ... Proteins were digested by incubation with sequencing-grade modified trypsin (1/50 w/w; Promega, V5113) for 12 h at 37°C ...