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Citations for Promega :
851 - 900 of 1643 citations for Recombinant Rabbit CD274 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... RBD proteins in culture media were measured in Nano-Glo buffer (Promega N1110) supplemented with Nanoluciferase substrate and 11S protein according to the manual ...
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bioRxiv - Cell Biology 2023Quote: ... Protein samples were then digested overnight at 37°C with Trypsin Gold (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: A whole-cell protein extract from human K562-cells (Promega, V6941, lot 444583) was dissolved in 50 mmol/L Tris and 6.5 mol/L urea ...
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bioRxiv - Biochemistry 2023Quote: ... and digestion of purified crosslinked protein with sequencing grade modified porcine trypsin (Promega), tryptic peptides were separated by reverse phase XSelect CSH C18 2.5 um resin (Waters ...
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bioRxiv - Microbiology 2023Quote: ... HiBiT-tagged proteins were detected using the Nano-Glo HiBiT blotting system (Promega) according to the manual ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were digested with Lys-C (Wako) and sequencing-grade trypsin (Promega, V5117) (1 μg of each in 20 μL of 50 mM TEAB ...
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bioRxiv - Biochemistry 2023Quote: ... In vitro protein synthesis by the coupled transcription and translation (TnT) system (Promega) was carried out in the presence of 35S methionine in either wheat germ or rabbit reticulocyte lysates ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were digested with 0.4 µg of sequencing grade modified trypsin (Promega, #V5111) in 40 µl Hepes/NaOH ...
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bioRxiv - Cell Biology 2023Quote: ... the proteins were digested with 1 µg of trypsin/Lys-C mix (Promega) for 16 h at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were digested using 0.005 µg/µl trypsin (sequencing grade modified trypsin; Promega) in 40 mM NH4HCO3/10% ACN at 4°C for 20 minutes followed by 16 h at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... were digested with a modified MS grade trypsin (Promega; 1:5 enzyme/protein) overnight at 37 °C ...
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bioRxiv - Plant Biology 2024Quote: ... The proteins were proteolytically digested on the column by incubation with trypsin (Promega) overnight at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were expressed using TnT® Quick Coupled Transcription/Translation System (Promega L1170), according to manufacturer’s instructions and stored at -80°C.
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bioRxiv - Immunology 2024Quote: ... and IgG captured and eluted from Magne Protein A beads (Promega, Madison, WI) as previously described 18,19 ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were digested with 0.8 µg/µL trypsin solution (proteomics grade trypsin, Promega). Peptides were then eluted in 65 µl Digestion buffer (50 mM TEAB pH 8.5) ...
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bioRxiv - Plant Biology 2024Quote: ... HALO-TF protein was subsequently incubated with 10 ug of MagneHALO beads (Promega) for 1 hour rotating at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... In-solution on-bead digestion of proteins was performed using Immobilized trypsin (Promega, sample-enzyme solution ratio of 94 (v/v) ...
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bioRxiv - Molecular Biology 2019Quote: ... Horseradish peroxidase-conjugated goat anti-mouse or goat anti-rabbit IgG (Promega; 1:10,000) was incubated for 1 h at room temperature and then washed four times with PBS-Tween for 20 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Control immunoprecipitations (mock) were performed with a non-specific antibody (Anti-Rabbit IgG, Promega). Western blot analyses were performed using the following antibodies ...
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bioRxiv - Plant Biology 2020Quote: ... Antigen on the membrane was visualized with horseradish peroxidase-coupled anti-rabbit IgG (Promega) for VHA-a1 and VHA-a3 ...
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bioRxiv - Biochemistry 2020Quote: ... and goat anti-rabbit IgG (H+L) antibody conjugated to HRP (Promega, catalog # W4018). Immunoblots were imaged using iBright 1500 (ThermoFisher ...
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bioRxiv - Biophysics 2020Quote: ... In vitro translation of the mRNA was carried out using rabbit reticulocyte lysate (Promega) supplemented with canine rough microsomes (tRNA probes ...
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bioRxiv - Cell Biology 2021Quote: ... HRP conjugated anti-rabbit (W401B) and anti-mouse (W402B) secondary antibodies were from Promega. CB-5083 was a gift from Cleave Biosciences and Bortezomib was from Selleckchem ...
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bioRxiv - Microbiology 2021Quote: ... Anti-mouse and anti-rabbit HRP-conjugated secondary antibodies were purchased from Promega (USA). Alexa Fluor 488 and Alexa Fluor 594 antibodies were purchased from Invitrogen (USA) ...
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bioRxiv - Neuroscience 2020Quote: ... overnight at 4°C and HRP-conjugated anti-rabbit antibody (1:2,000, W4011, Promega) for 1 hr at room temperature followed by ECL signal amplification and chemiluminescence detection (SuperSignal West Pico Chemiluminescent Substrate ...
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bioRxiv - Bioengineering 2022Quote: ... Horseradish peroxidase-conjugated anti-rabbit IgG (H + L) secondary antibody (W401B; Promega, Madison, WI) was used at a dilution rate of 1:5,000 for anti-TNF-α or -IL- 1β blotting ...
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bioRxiv - Cancer Biology 2022Quote: ... Anti-mouse (W4028) and anti-rabbit (W4018) HPR-conjugated secondary antibodies were from Promega. Anti-Mouse Alexa Fluor 488 (A32790) ...
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bioRxiv - Cancer Biology 2022Quote: ... Anti-mouse (W4028) and anti-rabbit (W4018) HRP conjugated secondary antibodies were from Promega. Anti-mouse Alexa® Fluor 488 (#A32790 ...
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bioRxiv - Molecular Biology 2019Quote: ... whereas Control Firefly mRNA was provided with Rabbit Reticulocyte Lysate System (Promega Cat#L4960). In vitro reaction was translated for 15 minutes at 37°C and stopped with the addition of 100 µg of cycloheximide and stored on ice prior to luciferase assay.
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bioRxiv - Pathology 2021Quote: ... and anti-rabbit IgG antibody coupled to horseradish peroxidase (Promega France, W401B, 1/2500) and revealed with the Western Lightning chemiluminescence reagent Plus (PerkinElmer Life Sciences) ...
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bioRxiv - Molecular Biology 2021Quote: In vitro translation reactions were performed using nuclease-treated rabbit reticulocyte lysate (RRL) (Promega) following the manufacturer’s protocol for non-radioactive luciferase reactions ...
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bioRxiv - Immunology 2021Quote: ... adding rabbit anti-chicken IgY which HRP-conjugated secondary antibodies (Promega, Madison, WI, USA) in the dilution of 1:5000 and incubated at 37°C for an hour ...
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C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: Anti-mouse and anti-rabbit Abs conjugated with horseradish peroxidase (HRP) were from Promega Biotec (Madison ...
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bioRxiv - Biochemistry 2023Quote: ... followed by the secondary anti-rabbit HRP-conjugated antibody (W401B, Promega, Madison, Wi, USA), diluted 1:10.000 in 5% BSA TBS-T ...
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bioRxiv - Microbiology 2023Quote: ... Antibodies were visualized by using anti-rabbit immunoglobulin G-alkaline phosphatase secondary antibodies (Promega) and the CDP-star detection system (Roche Diagnostics ...
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bioRxiv - Immunology 2023Quote: ... Unpurified PCR product was used as input to rabbit reticulocyte transcription translation system (Promega) and Nano-Glo HiBit Lytic Detection System (Promega Cat No ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit and mouse anti-β-Gal (MP Biomedics #559761 and Promega #Z378A, 1:1000), rabbit and mouse anti-pH3 (Merck Millipore #06-570 and Cell signal technology #9796 ...
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bioRxiv - Cell Biology 2023Quote: ... using the rabbit reticulocytes lysates Nuclease-treated kit and the transcendTM tRNA from Promega, as per the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies used for western blot include anti-Rabbit-HRP (1:20000, Promega W4011); anti-Mouse-HRP (1:20000 ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by overnight incubation with primary antibodies rabbit anti-HaloTag (1:500, G9281, Promega) and mouse anti-LaminB1 (0.5ug/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... Secondary antibodies used: anti-rabbit IgG (H+L) HRP conjugate (Promega W4011, 1:2,500); anti-mouse IgG (H+L ...
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bioRxiv - Plant Biology 2019Quote: ... In the next step proteins were digested overnight using 100 ng/µl trypsin (Promega) at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins bound to streptavidin-sepharose matrix were digested with trypsin (Promega, Madison, WI, USA) during 16 h or eluted for western blot analysis ...
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bioRxiv - Genetics 2021Quote: The in vitro protein translations were performed using the TNT-assay (L4610) from Promega according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: Tryptic digestion of proteins was carried out using porcine trypsin (Promega, GmbH, Mannheim, Germany). Trypsin solution prepared in 100 mM Ambic and 1 mM CaCl2 at a 0.01 μg/μl concentration and 100 μl added onto each sample for a final concentration of ~1.2 μg trypsin per sample containing 30 μg of total protein (trypsin to sample ration 1:25) ...
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bioRxiv - Molecular Biology 2022Quote: ... The proteins were digested in 1 M urea with modified trypsin (Promega, Madison, WI) at a 1:50 enzyme-to-substrate ratio ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were digested with Lys-C (Wako Chemicals) overnight and trypsin (Trypsin Gold, Promega) for 6 hours.
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bioRxiv - Molecular Biology 2020Quote: ... Protein pellets were resuspended in 8 M urea buffer combined with protease inhibitor (Promega) and purified using the chloroform-methanol precipitation method (Wessel & Flügge ...
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bioRxiv - Biophysics 2022Quote: ... The purified Kif5B protein was incubated with 10 µM HaloTag PEG-Biotin ligand (Promega) for 30 min on ice to produce a final construct of Kif5B homodimer with two C-terminal biotin tags ...
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bioRxiv - Systems Biology 2020Quote: ... Human cell lysate (MS Compatible Human Protein Extract, Digest, V6951) were purchased from Promega.