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Citations for Promega :
1051 - 1100 of 4976 citations for Onion Yellow Dwarf Virus OYDV PCR Kits since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: The amplified products underwent purification using Quick PCR purification columns (Promega, Madison, WI, USA) and were subsequently sequenced with the Big Dye Terminator Cycle Sequencing Ready Reaction Kit on an Applied Biosystems analyzer (Applied Biosystems ...
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bioRxiv - Developmental Biology 2023Quote: ... The PCR products were digested by direct addition of 0.5 µl of HindIII (Promega) and incubation for 1 h at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were amplified by 16 cycles of PCR with GoTaq Flexi DNA Polymerase (Promega), purified using Agencourt AMPure XP (Beckman Coulter ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR-amplified products were then cloned into the pGEM-T Easy Vector (Promega) and subjected to sequencing for further analysis.
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bioRxiv - Molecular Biology 2023Quote: ... Parental plasmids were selectively linearised from the PCR product via digestion with Dpnl (Promega) in lx MULTI-CORE buffer for 1hr at 37°C and the digestion product was immediately transformed into Stbl3 competent bacteria.
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bioRxiv - Biochemistry 2023Quote: Quantitative PCR: Taq qPCR was performed using SYBR Green GoTaq qPCR master mix (Promega) according to manufacturer’s instructions on a LightCycler 96 SW 1.0 (Roche Diagnostics GmbH ...
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bioRxiv - Biophysics 2023Quote: ... a regular PCR was run on a thermocycler using Taq polymerase master mix (Promega). Gene knockout was thereby confirmed by gel electrophoresis ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic sites encompassing targeted guide regions were amplified by PCR using GoTaq polymerase (Promega) and sequenced ...
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bioRxiv - Neuroscience 2024Quote: ... Quantitative PCR was performed using a GoTaq qPCR master mix per manufacturer’s instructions (Promega). All gene expression levels were normalized against that of GAPDH.
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bioRxiv - Cancer Biology 2024Quote: ... The PCR product was gel-purified and cloned into the pGL3-control plasmid (Promega) at the XbaI and SalI sites ...
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bioRxiv - Microbiology 2024Quote: ... Each 25 μl PCR reaction was prepared using GoTaq G2 Flexi DNA Polymerase (Promega) and contained 0.5 U μl−1 GoTaq G2 Flexi DNA Polymerase ...
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bioRxiv - Neuroscience 2024Quote: ... and PCR was performed with GoTaq G2 Flexi DNA Polymerase (Promega, catalogue no. M7805) with primers to amplify DNA sequence around the sgRNA targeting sites within the gene of interest (Supplementary Table 6) ...
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bioRxiv - Cell Biology 2024Quote: ... DNA was cleaned up with Promega Wizard SV gel and PCR Cleanup System (Promega). The inserts and the backbone were mixed in a 3:1 ratio and incubated with the 2x NEBuilder HiFi DNA assembly enzyme mix (New England Biolabs ...
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bioRxiv - Physiology 2024Quote: ... the PCR products were ligated into the pGEM-T Easy Vector (Promega, Madison, WI), and the sequences were confirmed by Sanger sequencing ...
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bioRxiv - Plant Biology 2024Quote: ... PCR products were purified and cloned into the pGEMT vector (Promega, Madison, WI, USA). A minimum of 10 clones per PCR product were then sequenced by the Sanger method to characterize the edited alleles.
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bioRxiv - Immunology 2024Quote: ... the Renilla luciferase sequence was PCR amplified from plasmid pRL-TK-Renilla (Promega, E2241) and the hPEST sequence was PCR amplified from plasmid pcDNA3.1(+)GUG-nLuc-3XFLAG-CL1/PEST (Addgene plasmid #127317 ...
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bioRxiv - Plant Biology 2024Quote: ... Genes of interest were amplified from template DNA by PCR using GOTaq polymerase (Promega) according to the recommendations of the supplier ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was purified using the Wizard SV Gel and PCR clean-up system (Promega), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The PCR product was then cloned into the TOPO II dual promoter vector (Promega) and the riboprobe synthesized (linearized with SpeI ...
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bioRxiv - Bioengineering 2024Quote: ... and purified with the Wizard SV-Gel and PCR Clean-Up System (Promega, USA). DNA was then mixed with streptavidin (S0677 or S4762 ...
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bioRxiv - Genomics 2024Quote: ... PCR reactions were set up in 25μl using GoTaq® DNA Polymerase (Promega, USA) and 30-50 ng of template DNA ...
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bioRxiv - Synthetic Biology 2024Quote: ... and purified with the Wizard SV-Gel and PCR Clean-Up System (Promega, USA).
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bioRxiv - Immunology 2024Quote: ... The digested PCR product was then cloned into a pGEM-T Easy plasmid (Promega) and verified by Sanger sequencing ...
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bioRxiv - Genomics 2024Quote: ... PCR reaction comprised of 1x GoTaq® Green Master Mix (Promega Corp., WI, USA), 0.5 μM of GP5+ and GP6+ primers and 50 ng of genomic DNA ...
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bioRxiv - Genetics 2024Quote: ... High-quality gDNA of clones verified by microscopy and PCR was purified (Promega A1120) and each genotyping PCR fragment was sequenced ...
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bioRxiv - Biochemistry 2024Quote: ... purified PCR products of B4GALT5 were cloned into pCRScript Amp SK (Promega, Madison, WI) and transformed into Ε ...
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bioRxiv - Cell Biology 2020Quote: ... caspase8 Kit (Promega company); Caspase3 / 7 Kit (Promega company).
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bioRxiv - Molecular Biology 2020Quote: ... or Maxwell kit (Promega). Following RNA extraction ...
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bioRxiv - Genetics 2023Quote: ... the GoScript Kit (Promega) was used to make cDNA and qRT-PCR performed on a BioRAD c1000 Touch thermocycler.
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bioRxiv - Plant Biology 2020Quote: ... PCR amplifications for genotyping were performed using GoTaq Green Master Mix (Promega, Madison, WI, USA), with the resulting products subjected to 3% agarose gel electrophoresis (Supplemental Fig ...
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bioRxiv - Cell Biology 2020Quote: ... The PCR product was digested with XhoI and NcoI and ligated into pGL3-control (Promega). The resulting plasmid ...
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bioRxiv - Cell Biology 2020Quote: ... The linear PCR products were circularised by ligation with T4 DNA ligase (Promega Cat#M180A) (O/N ...
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bioRxiv - Cell Biology 2020Quote: ... All PCR reactions on cDNA were performed using the GoTaq DNA polymerase (Promega, Madison, WI) unless otherwise noted ...
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bioRxiv - Cell Biology 2020Quote: ... This PCR reaction was carried out with GoTaq G2 hot start DNA Polymerase (Promega, F549L). All PCR reactions were in 96-well plates with a Freedom EVO 200 (Tecan ...
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bioRxiv - Neuroscience 2021Quote: ... The reverse-transcribed cDNA was subjected to quantitative PCR using GoTaq qPCR Master Mix (Promega) and the StepOnePlus™ Real-time PCR system (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... Successful integration was confirmed by diagnostic PCR using GOtaq Hot Start Green Master Mix (Promega). For primer sequences see Supplementary file 1.
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bioRxiv - Developmental Biology 2022Quote: ... or produced by PCR amplification of a fragment subsequently ligated in pGEMT Easy vector (Promega). Primers used to amplify the fragment of interest are listed in Table S2 ...
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bioRxiv - Molecular Biology 2020Quote: ... The isolated RNA was used for cDNA synthesis using the Access RT-PCR system (Promega) with AMV reverse transcriptase ...
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bioRxiv - Genetics 2020Quote: ... The PCR products were cloned into pGEM®-T Easy vector (Promega, cat. no: A1360), and ten colonies were selected for sequencing.
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bioRxiv - Cell Biology 2021Quote: ... were amplified by PCR and cloned into the pGL3-Basic luciferase reporter vector (Promega, E1751) using the KpnI and XhoI sites [promoter sequence defined using the Eukaryotic promoter database (https://epd.epfl.ch//index.php)] ...
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bioRxiv - Microbiology 2020Quote: ... quantitative real-time PCR was carried out according to the GoTaq qPCR Master Mix (Promega) with 20 µl of a reaction mixture containing gene-specific primers (Supplementary Table 3) ...
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bioRxiv - Neuroscience 2021Quote: ... PCR reaction (25 μl) contained 12.5 μl of Go-Taq Master Mixes (Promega, Milan, Italy), 1 µl (25 pmoles ...
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bioRxiv - Neuroscience 2020Quote: ... PCR reactions were performed using 25ng bisulfite converted DNA using GoTaq® DNA Polymerase (Promega). The TA Cloning Kit (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: Sequences for generating probes were amplified from cDNA by PCR using GoTaq DNA Polymerase (Promega) on a BioRad T100 Thermal cycler ...
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bioRxiv - Biochemistry 2021Quote: ... Quantitative RT-PCR was performed using GoTaq® Probe qPCR and RT-qPCR Systems (Promega) in a StepOne™ Real-Time PCR System (Thermo Fisher) ...
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bioRxiv - Microbiology 2021Quote: ... pRRL.sin.cPPT.SFFV/Firefly-IRES-PuromycinR.WPRE was obtained by amplification of Firefly by PCR from pGL4 (Promega) and cloned into BamHI-XhoI-digested pRRL.sin.cPPT.SFFV/E2-crimson-IRES-PuromycinR.WPRE ...
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bioRxiv - Physiology 2021Quote: ... PCR products were purified with the Wizard SV Genomic DNA Purification System (Promega, Cat# A2361), and sent in to a commercial sequencing platform (Eurofins Genomics ...
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bioRxiv - Plant Biology 2020Quote: ... collected supernatant was used as a template in a standard PCR reaction using GoTaq (Promega) with Cas9-specific primers or primers to amplify the gRNA(s ...
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bioRxiv - Microbiology 2020Quote: ... PCR was conducted with the following reaction conditions: 0.1 U/µL Gotaq (Promega, Madison, WI), 1X Gotaq buffer ...
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bioRxiv - Cell Biology 2021Quote: ... The amplified product was purified using Wizard SV Gel and PCR Clean-up system (Promega) and inserted into T-Vector pMD20 (Takara Bio ...