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Citations for Promega :
1301 - 1350 of 4976 citations for Onion Yellow Dwarf Virus OYDV PCR Kits since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: The qRT-PCR reaction was carried out with GoTaq®qPCR Master Mix (Promega, Madison, WI, USA) on a CFX96 instrument (Bio-Rad ...
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bioRxiv - Genomics 2021Quote: ... DNA was resuspended in 30 uL PCR grade water and quantified by Quantus™ Fluorometer (Promega Inc). The PCR product pools were sequenced by short read sequencing as described by 2.3.1.
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bioRxiv - Developmental Biology 2022Quote: ... The amplified PCR fragments were subcloned into the pGEM T Vector system (Promega Corporation, Madison, WI, USA) and sequenced to confirm each allele.
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bioRxiv - Cell Biology 2022Quote: ... P2-2: -212- -826) were cloned through PCR into the pGL4.20 luciferase reporter vector (Promega, Madison, WI) using C57BL/6 genomic DNA as a template ...
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bioRxiv - Bioengineering 2022Quote: ... the EGFP coding sequence was PCR amplified from the plasmid pDestattB_ubi:EGFP using GoTaq® DNA Polymerase (Promega) with attB-P2A-EGFP-F and pA-r primers (Supplementary Table S3) ...
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bioRxiv - Cell Biology 2022Quote: ... and aliquots of the cells were analyzed by genomic PCR using GoTaq DNA polymerase (Promega, WI, USA) and specific primers (F_C2 ...
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bioRxiv - Physiology 2022Quote: ... the PCR products were subjected to T/A cloning (pGEM®-T Vector Systems; Promega, Fitchburg, USA) with subsequent Sanger sequencing (Sanger et al ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were subsequently cloned into vectors using pGEM-T Easy Vector System II (Promega, Cat# A1380) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... and transferred into 0.2 ml PCR tubes containing 2μl of lysis buffer (0.7% Triton X-100, 2U/μl RNasin (Promega), 1μl of oligo-dT30VN primer (10 μM 5’-aagcagtggttatcaacgcagagtact30vn-3’ ...
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bioRxiv - Microbiology 2023Quote: ... as well as 1kB DNA ladder were purchased from New England Biolabs while GoTaq PCR reagents (Promega) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... qPCR was performed on a CFX96 q–PCR System using SYBR Green qPCR master mix (Promega, USA). GAPDH was used as the internal control ...
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bioRxiv - Plant Biology 2023Quote: ... AP1 (AT1G69120) cDNA was PCR-amplified using specific primers and inserted into XbaI/BamHI digested pSP64 (Promega) vector ...
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bioRxiv - Microbiology 2023Quote: ... The 3809-bp PCR product was purified and cloned into pGEM-T-Easy (Promega, Medison WI, USA) to yield pGEM ...
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bioRxiv - Genetics 2023Quote: ... PCR was performed in a 30 µL reaction volume containing 1X Colorless GoTaq Reaction Buffer (Promega, #M792B), 200 µM dNTPs (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... All PCR analyses were performed on 0.5 ng cDNA using 0.5 U GoTaq® DNA polymerase (Promega). Quantification of PCR products was performed using the Image Lab software (BioRad ...
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bioRxiv - Cancer Biology 2023Quote: ... Then the eluted DNA was subjected to real-time PCR analysis with Eastep qPCR Master Mix (Promega) and promoter-specific primer mixtures ...
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bioRxiv - Genomics 2023Quote: ... Agar was removed from the DNA using the Wizard SV Gel and PCR Clean-Up System (Promega). Transposon-directed insertion site sequencing (TraDIS ...
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bioRxiv - Genetics 2022Quote: ... The digested plasmid was purified using the Wizard SV Gel and PCR Clean-Up System (Promega A9281). The zebrafish exorh promoter region was amplified from pCK029 (this manuscript ...
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bioRxiv - Genetics 2022Quote: Quantitative PCR was performed on the Roche LightCycler 96 instrument by using GoTaq qPCR Master Mix (Promega) with 1µl cDNA template and 0.5µl of 10mM each primer in a total volume of 25µl reaction ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The amplified products were gel-purified with the Wizard SV Gel and PCR Clean-Up System (Promega) and then cloned into the pGEM-T Easy Vector (Promega) ...
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bioRxiv - Genomics 2023Quote: ... PCR validation reactions amplification were performed using GoTaq® DNA polymerase (Promega, 1.2 units/50 μl reaction), Colorless GoTaq® Reaction Buffer ...
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bioRxiv - Biochemistry 2023Quote: ... and used 1 μL of cDNA for 10 μL of PCR using 2x GoTaq master mix (Promega) and 250 nM of forward and reverse primer sets with conditions recommended by the manufacturer ...
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bioRxiv - Physiology 2023Quote: ... Each 10 µl PCR reaction consisted of 5 µl of GoTaq® qPCR Master Mix (Promega Corporation), 0.5 µl of 10 µM forward primer ...
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bioRxiv - Developmental Biology 2023Quote: ... A-tailed PCR products were ligated with digested linear pGEM®-T Vector (Promega, Cat. No. A3600), followed by transformation with One Shot™ TOP10 Chemically Competent E ...
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bioRxiv - Cell Biology 2023Quote: ... The inserts and plasmid backbones were purified with Promega Wizard SV gel and PCR Cleanup System (Promega). Purified inserts and backbones were mixed in a molar 3:1 ratio ...
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bioRxiv - Neuroscience 2023Quote: ... PCR products were further digested by TAQI (GoTaq® G2 Flexi DNA Polymerase; Promega, cat. no M7805) to assess if the point mutation is or not present ...
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bioRxiv - Cancer Biology 2024Quote: ... Real-time quantitative PCR was performed for murine Clec18a and Gapdh with GoTaq qPCR master mix (Promega) on the CFX384 system (Bio-Rad) ...
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bioRxiv - Immunology 2024Quote: ... PCR was done on gDNA from at least 6 individual larvae using GoTaq Green Master Mix (Promega) (Supp Fig 1A ...
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bioRxiv - Molecular Biology 2024Quote: ... The 966-bp amplicon was purified using the Wizard SV Gel and PCR Clean-Up System (Promega) and subcloned into the TA cloning vector pGEM-T easy (Promega) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCRs were performed under standard conditions for High Performance GoTaq® G2 Flexi DNA Polymerase (Promega; M7801), with the addition of 5x combined enhancer solution [35] per reaction ...
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bioRxiv - Genomics 2024Quote: ... The master mixtures consisted of 1 x Taq&Go G2 Hot Start colorless PCR Master Mix (Promega), 0.5 μM of each primer ...
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bioRxiv - Molecular Biology 2024Quote: ... an initial PCR was performed using gDNA (100 ng) with 1x GoTaq Green Master Mix (Promega #M7123), and 0.5 μM of each primer ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and target bands were purified using the Wizard SV Gel and PCR Clean-Up System (Promega A9282).
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bioRxiv - Immunology 2024Quote: ... Quantitative real-time PCR assays were carried out by using GoTaq® qPCR master mix (Promega, Germany) and QuantStudio™ 5 (Thermo fisher scientific ...
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bioRxiv - Plant Biology 2024Quote: ... the gel-eluted PCR products from T0 lines were cloned into pGEM-T Easy vector (Promega, Germany) or pJET1.2 blunt end vector (Thermo Scientific™ ...
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bioRxiv - Neuroscience 2024Quote: ... and subsequently used for a qRT-PCR with GoTaq® qPCR Master Mix (A6002, Promega, WI, USA) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Detection of MpFV in aphids was carried out in RT-PCR assays using Green GoTaq Polymerase (Promega) and the MpFV-Hel primers with an expected PCR product of 361 bp (Table S1) ...
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bioRxiv - Microbiology 2024Quote: ... Reverse transcription PCR was conducted with the ImProm-II reverse transcription system (Promega Corporation, Madison, WI, USA) using 1 µg of RNA in 20 µL reaction mixtures ...
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bioRxiv - Microbiology 2024Quote: ... that included (for 1 well): 5 μl of the SYBR Green Real-Time PCR master mix (Promega), 1.25 μl of the forward and reverse primer (standardized KiCqStartTM primers ...
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bioRxiv - Genomics 2024Quote: ... We then purified the PCR products with 95 µl ProNex Size-Selective Purification System beads (Promega, NG2001) following the vendor’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... The PCR amplicons were then inserted into pGEM vectors using the pGEM-T Easy Vector system (Promega) and 40 (CRISPR-edited ...
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bioRxiv - Neuroscience 2024Quote: ... Purification was performed by ethanol precipitation and Wizard SV Gel and PCR Clean-Up System (A9281; Promega). DNA fragments encoding Dh31 and Dh44 were subcloned into the SgfI and PmeI sites of pF25A ICE T7 Flexi Vector (L1061 ...
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bioRxiv - Genomics 2024Quote: ... The CaGHV-1 ORF50 promoter fragments were PCR amplified and cloned into pGL4.15 luciferase reporter vector (Promega) using In-Fusion cloning ...
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bioRxiv - Cell Biology 2024Quote: ... The inserts and plasmid backbones were purified with Promega Wizard SV gel and PCR Cleanup System (Promega). Purified inserts and backbones were mixed in a molar 3:1 ratio ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal quantities of tumour genomic DNA (100 ng) were amplified by PCR with GoTaq (Promega cat# M7123) using primers to detect Rb1 recombination ...
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bioRxiv - Developmental Biology 2024Quote: ... The 10µl reaction mixture contained 2x SYBR Green PCR Master Mix (Promega, GoTaq qPCR Master, cat.no. A6002), 2 µM primer and 1 μl of diluted cDNA ...
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bioRxiv - Genomics 2021Quote: ... was extracted from tissue samples using a commercial kit (Wizard R Genomic DNA Purification Kit, Promega) following manufacturer’s instructions ...
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Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substratesbioRxiv - Biochemistry 2020Quote: ... with the TNT kit (Promega: L1770). Each substrate was amplified with primers by PCR to allow T7-dependent transcription of the PCR product or transcribed directly if plasmids contained a T7 promoter ...
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bioRxiv - Microbiology 2021Quote: ... or Luciferase Assay System kit (Promega) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2020Quote: ... the PureYield™ MidiPrep Kit (ProMega) and NucleoBond Xtra™ Midi/Maxi Kit (Macherey & Nagel ...