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Citations for Promega :
1001 - 1050 of 3874 citations for Mouse Chitinase 1 CHIT1 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... The reactions were performed using the S30 T7 High-Yield Protein Expression System (Promega). 3 μl of peptide solution were added to reaction mixtures to get a final concentration respectively of 32 μg/ml or 512 μg/ml in a final volume of 15 μL in nuclease-free PCR tubes ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 ng/μl of mRNA and 6 ng/μl QuantiLum Recombinant Luciferase protein (Promega) were coinjected ...
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bioRxiv - Biochemistry 2024Quote: ... the blotted proteins were developed using Nano-Glo® In-Gel Detection System (Promega) and scanned with a ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Biochemistry 2024Quote: ... The HaloTag-JF503-SWSAP1 was quantified by using recombinant HALO-GFP protein standard (Promega) pre-incubated with JF503 at a 1:1 molar ratio and titrated into single molecule buffer (20 mM Tris pH7.5 ...
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bioRxiv - Systems Biology 2023Quote: ... dark) and digestion using 0.015 µg Trypsin for 1.5 µg protein (Trypsin Gold, Promega, Fitchburg ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested by trypsin (Trypsin Gold, Mass Spectrometry Grade, Promega; protein:enzyme (w/w) ratio – 100:1 ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were then digested into peptides with sequencing grade modified trypsin (Promega V5113) at 37 °C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were digested for 18 h at 37 °C with 2 μg trypsin (Promega). After digestion ...
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bioRxiv - Biochemistry 2023Quote: Eluted proteins were digested with Lys-C (FUJIFILM Wako) and trypsin (Promega, Madison, WI) according to the PTS protocol21 ...
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bioRxiv - Biophysics 2023Quote: ... the desired volume of protein solution was incubated with Factor Xa (Promega, Madison, WI). All experiments were carried out in a tris buffer (150 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... and proteins were further digested by the addition of 2 μg trypsin (Promega #V5113) for 4 hours at room temperature on a shaker ...
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bioRxiv - Physiology 2024Quote: ... Chemiluminescent detection of in vitro translated protein was performed following the manufacturer’s protocol (Promega).
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Direct and indirect regulation of β-glucocerebrosidase by the transcription factors USF2 and ONECUT2bioRxiv - Molecular Biology 2024Quote: ... Proteins were digested overnight at 37 °C with LysC (Wako Chemicals) and trypsin (Promega) in a 1/100 enzyme-to-substrate ratio ...
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bioRxiv - Microbiology 2024Quote: The CHIKV ORF encoding for viral structural proteins was cloned into pCI-Neo (Promega) to obtain pCI-neo-CHIKV ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were digested in-gel using trypsin (500 ng/band, Gold, Promega, Ref V5280). Digest products were dehydrated in a vacuum centrifuge and reduced to 3 μL ...
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bioRxiv - Molecular Biology 2024Quote: ... 3 volumes of TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega) was incubated with 2 volumes of 100 ng/μL purified plasmid at 25°C for 2 hours and used immediately.
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bioRxiv - Neuroscience 2024Quote: ... Proteins were then digested overnight at 37°C with 3 μg Trypsin/LysC (Promega). The digest was then acidified with formic acid and desalted (C18 HyperSep columns) ...
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bioRxiv - Biochemistry 2024Quote: ... Endogenous protein levels were quantified using the Nano-Glo HiBiT Lytic Detection System (Promega) as described in manufacturer’s manual ...
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bioRxiv - Biochemistry 2024Quote: Limited proteolysis experiments for WT FL-HsFN3K protein were done using either Thermolysin (Promega) or Chymotrypsin (Sigma ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The proteins were then digested in situ with sequencing-grade trypsin (Promega, Madison, WI), following the method described by Pérez et al.106 ...
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bioRxiv - Genomics 2021Quote: ... 1 U μl−1 RNasein (Promega), 0.1% IGEPAL CA-630 (Sigma)) ...
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bioRxiv - Immunology 2021Quote: ... mouse tail DNA was amplified using GoTaq G2 Green Master Mix (Promega, cat. M7823) or KAPA Taq PCR Kit (Takara Bio ...
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bioRxiv - Cell Biology 2020Quote: ... The peroxidase-conjugated secondary antibodies were goat anti-mouse IgG (715-035-151, Promega) and anti-rabbit IgG (711-035-152 ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were probed with either goat anti-mouse IgG-HRP (Promega, catalogue no. W4021) or goat anti-rabbit IgG-HRP (catalogue no ...
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bioRxiv - Cell Biology 2021Quote: ... HRP conjugated anti-rabbit (W401B) and anti-mouse (W402B) secondary antibodies were from Promega. CB-5083 was a gift from Cleave Biosciences and Bortezomib was from Selleckchem ...
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bioRxiv - Microbiology 2021Quote: ... Anti-mouse and anti-rabbit HRP-conjugated secondary antibodies were purchased from Promega (USA). Alexa Fluor 488 and Alexa Fluor 594 antibodies were purchased from Invitrogen (USA) ...
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bioRxiv - Cancer Biology 2022Quote: ... Anti-mouse (W4028) and anti-rabbit (W4018) HPR-conjugated secondary antibodies were from Promega. Anti-Mouse Alexa Fluor 488 (A32790) ...
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bioRxiv - Cancer Biology 2022Quote: ... Anti-mouse (W4028) and anti-rabbit (W4018) HRP conjugated secondary antibodies were from Promega. Anti-mouse Alexa® Fluor 488 (#A32790 ...
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C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: Anti-mouse and anti-rabbit Abs conjugated with horseradish peroxidase (HRP) were from Promega Biotec (Madison ...
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bioRxiv - Genetics 2020Quote: ... Total RNA (0.5 µg) was reverse transcribed with mouse myeloblastosis virus reverse transcriptase (Promega) under standard conditions using hexanucleotide random primers according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: The mouse Il12b promoter (−355 to +55) was cloned into the pGL4.10 vector (Promega). Motif mutations were introduced using the GENEART site-directed mutagenesis system (Invitrogen) ...
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bioRxiv - Biochemistry 2024Quote: ... 24 hours after transient transfection with mouse or human ENPP3 via Fugene 6 (Promega), the media was gently removed and replaced with serum-free DMEM supplemented with 1% insulin-transferrin-selenium-sodium pyruvate (ThermoFisher ...
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bioRxiv - Genetics 2023Quote: RNA was isolated from mouse liver using a Maxwell 16 LEV simplyRNA kit (Promega). For the American vs standard diet comparison strand-specific mRNA libraries were generated using the TruSeq Stranded mRNA protocol (Illumina ...
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bioRxiv - Biochemistry 2022Quote: ... but the secondary antibodies were goat anti-mouse HRP conjugated antibody (Promega Cat #W4021) and goat anti-rabbit HRP conjugated antibody (Promega Cat # W4011) ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested overnight at 37 °C with Trypsin/Lys-C Mix (Promega, Charbonnières, France) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... proteins on the beads were digested by adding 400 ng trypsin/Lys-C mix (Promega) for 16 h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were cleaved by overnight digestion in porcine trypsin (Sequencing Grade Modified, Promega, Wisconsin). The tryptic peptides were extracted by using 5% acetic acid in 50% acetonitrile and vacuum-dried by speedvac ...
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bioRxiv - Microbiology 2020Quote: ... Protein digestion was carried out by adding 2 μg of trypsin solution (Promega, Charbonnières, France) to the alkylated proteins ...
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bioRxiv - Immunology 2021Quote: ... Total protein (100 μg) extracted from each sample was digested with Trypsin Gold (Promega, USA) with the ratio of protein:trypsin = 30:1 and incubated at 37 °C for 16 h ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were digested overnight at 37 °C using trypsin digestion (Sequencing Grade Modified Trypsin, Promega) at pH 8.0 using a protein to protease ratio of 100:1 ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins from the gel pieces were digested with trypsin HPLC-grade (Promega AG, Dübendorf, Switzerland) overnight and the resulting peptide solutions were desalted on C18-based STAGE tips as previously described [43,44] ...
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bioRxiv - Biochemistry 2020Quote: A total of 100 μg protein was digested with Trypsin Gold (Promega, Madison, WI, USA) with the ratio of protein:trypsin = 30:1 at 37 °C for 16 h ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were subjected to on-filter proteolysis with Lys-C (Wako Chemicals) and trypsin (Promega). Tryptic peptides were eluted by centrifugation and acidified with trifluoroacetic acid prior to liquid chromatography-mass spectrometry (LC-MSMS ...
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bioRxiv - Physiology 2021Quote: ... the eluted proteins were digested with 10 μg/mL modified trypsin (Sequencing grade, Promega, USA) at 37 °C for 16 h ...
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bioRxiv - Cell Biology 2021Quote: ... The cleared supernatant was added to a column containing HisLink Protein Purification Resin (V8823, Promega) and his-tag fusion proteins were purified according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Protein preparation and luciferase– substrate interaction were conducted with a Luciferase Assay Report Kit (Promega). Luciferase activity was measured by a microplate reader (Thermo Scientific Varioskan Flash) ...
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bioRxiv - Systems Biology 2020Quote: ... Treated proteins were digested overnight at 37 °C using sequencing-grade trypsin (Promega, cat: V511C) at an enzyme-to-protein ratio of 1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins extracted were then processed with a precipitation-based digestion protocol using trypsin (Promega) at a ratio of 1:20 as described previously(Collins et al. ...
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bioRxiv - Biochemistry 2022Quote: ... and proteins were digested on-bead with trypsin (1.5 µg, in provided buffer, Promega Corp.) overnight at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: Halo fusion proteins were visualized in cells by staining with fluorescent Halo ligand (Promega GA1110). Cells were seeded on polyD lysine coated coverslips (neuVitro ...