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Citations for Promega :
1001 - 1050 of 2640 citations for 2 Amino 3 chloro 5 nitro 6 picoline since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... HeLa-HA cells were co-transfected using 3 μL of FuGENE HD (Promega) with 0.5 μg of recovery vector (i.e. ...
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bioRxiv - Developmental Biology 2024Quote: ... Purified 3’UTRs were ligated with the pmirGLO dual-luciferase vector (Promega, #E1330) via SacI and SalI restriction sites ...
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bioRxiv - Cell Biology 2024Quote: ... Lysates were sonicated and incubated with 3 units/L culture RQ1 DNAse (Promega) and 500 units/L culture Benzonase Nuclease (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... An equal volume of reconstituted Caspase- Glo 3/7 Reagent (Promega, Madison, WI) was added to each sample and mixed by repeated pipetting ...
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bioRxiv - Biochemistry 2021Quote: ... Genomic DNA was eliminated by incubating 2 μg of RNA with 2 U of RQ1 RNase-free DNase I (Promega) for 30 min at 30°C ...
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bioRxiv - Genetics 2022Quote: ... Vectors coding for repair template DNA of roughly 2 kb were generated from Drosophila Schneider 2 cell genomic DNA by PCR using GoTaq G2 Polymerase (Promega) and primer pairs appropriate for the desired region (Supplementary Table S1) ...
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bioRxiv - Systems Biology 2020Quote: ... Reduced proteins were alkylated and trypsin-digested for 1-2 hours in trypsin solution (2 mg/ml sequencing grade porcine trypsin (Promega)/100 mM Tris/10 mM iodoacetamide ...
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bioRxiv - Microbiology 2022Quote: 293T cells were co-transfected with pEGFP-N1 and plasmids expressing SARS-CoV-2 Spike (FL) or SARS-CoV-2 Spike-Δ19 for 24 h using FugeneHD (Promega). The cells were treated with DMSO or 10 µM 6-TG at 4 h post- transfection ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were diluted to a concentration of urea of 2 mol L-1 and digested overnight with the addition of 2 µl sequencing-grade trypsin (Promega). After digestion ...
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bioRxiv - Molecular Biology 2023Quote: Proteins bound to beads were reduced with 2 mM DTT in 2 M urea buffer and sequencing grade trypsin (Promega) was added to a final concentration of 5 ng/μl ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were then digested overnight with 2 µg of lysyl endopeptidase (Wako) at room temperature and further digested overnight with 2 µg trypsin (Promega) at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... 3.5 mM MgCl2, and 2 mM ATP (for nucleic acid substrates containing RNA, 2 U/μl of RNasin Ribonuclease Inhibitor (Promega)) for 30 min at 37 °C ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... The pellet was washed with 2 ml nuclei storage buffer and resuspended in 0.2 ml PBS (calcium and magnesium-free) with 2% BSA and 0.2 U/μl RNasin® Plus RNase inhibitor (Promega). Buffer composition was described before72 ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were transfected with 750 ng of the pMXs-IP vectors with 2.3 μl FuGENE 6 (Promega, E2691). The culture medium was replaced with fresh DMEM with 10% FBS and C2C12 cells were seeded at a concentration of 1×105 cells/well in a 12-well plate on day 3 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with a control Flag or HA-GSK-3βWT human plasmid using Fugene 6 (Promega #E2693) in a 2:1 ratio and incubated for 3 hours in a CO2 incubator ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were then transfected with 1500 ng pcDNA-FLAG-HA-sscGAS plasmid complexed with FuGene 6 reagent (Promega) according to manufacturer’s instructions or treated with FuGene 6 alone as a negative control ...
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bioRxiv - Neuroscience 2020Quote: ... pCAG-DCC:TDTOMATO and pCAG-DCCkanga:TDTOMATO plasmids (1 µg) were transfected into the plated U251 cells using FuGENE 6 (Promega) in Opti-MEM (Gibco ...
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bioRxiv - Immunology 2020Quote: ... were transfected with 4.5 µg of the pBabe-puro-NCAM140 mApple construct (NCBI reference sequence: NM_000615.6, transcript variant 1) using Fugene 6 Transfection Reagent (Promega). Supernatant containing viral particles was collected and concentrated using PEG-IT (System Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... Generation of infectious lentiviral particles and subsequent cell transduction was performed as previously described.26 FuGENE 6 (Promega) was used to transfect select plasmids ...
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bioRxiv - Microbiology 2021Quote: ... 293T cells were co-transfected with 1.5 mg of spike expression plasmids in pCDNA3 and 0.5 mg pmCherry-N1 using Fugene 6 and following the manufacturer’s instructions (Promega). Vero cells were treated with CellTracker™ Green CMFDA (5-chloromethylfluorescein diacetate ...
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bioRxiv - Molecular Biology 2022Quote: ... the proteins were digested overnight at 37 °C with 6 ng μL-1 of Asp-N (Promega, UK). Peptides were extracted in 2 % v/v formic acid ...
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bioRxiv - Molecular Biology 2021Quote: ... 32μg SIV4+ (49)and with or without 1μg of pcDNA3.1 Vpx expression plasmid using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... were co-transfected with 0.5 μg of spike expression plasmids in pCDNA3 using Fugene 6 and following the manufacturer’s instructions (Promega). Cell-cell fusion was measured using an Incucyte and determined as the proportion of green area to total phase area ...
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bioRxiv - Cell Biology 2020Quote: ... and the solution was added to OMEM (75 μL) containing 6 μL Fugene HD reagent (Promega, Madison, WI). The mixture was incubated at room temperature for 10 minutes and gently added to the growth medium on the cultured cells ...
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bioRxiv - Microbiology 2021Quote: ... Plates were incubated for 24 h and viability was measured following a 6 h incubation with CellTiterBlue (Promega). Viability was quantified by fluorescent measurement (Ex ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested off the beads by adding 6 μL of 1 μg/μL sequencing grade Trypsin (Promega), diluted in 50 mM acetic acid ...
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bioRxiv - Microbiology 2023Quote: Lentiviral particles were produced by transfection of HEK293T cells in T150 flasks using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... DF1 cells were transfected with the different RCAS retroviral plasmids using FuGENE 6 Transfection reagent (Promega, Cat. E2691), accordingly to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with 8 µg of pcDNA3.1-GFP-mNLS-K145Q using Fugene 6 Transfection reagent (Promega, E2692) per manufacturer protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was assessed 6 days post-treatment using a Cell-TiterGlo 3D Cell Viability assay (Promega, G9683), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were transfected with LDLR-GFP (WT, C75W, and C75W/C34W) for 48 h with Fugene 6 (Promega). Cells were starved in 5% LPDS media (Millipore Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... were transfected into NCCIT cells (ATCC) at ∼70% confluency using a 6:1 ratio of Fugene HD (Promega) for 48 hours in ATCC-formulated RPMI media ...
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bioRxiv - Cancer Biology 2022Quote: ... 1mg of the plasmid was transfected to 293T cells (ATCC #CRL-11268) using FuGENE 6 Transfection Reagent (Promega) to produce the retroviruses ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with plasmid DNA by the calcium phosphate or lipofection method using FuGene 6 (Promega, WI) (Yoshida et al. ...
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bioRxiv - Neuroscience 2023Quote: HEK293T cells were transfected with either Tau-encoding or control constructs using Fugene 6 (Promega, cat. no. E2691) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... methylase was amplified using M.SssI_HindIII forward and reverse primers (Sup Table 6) cloned into pGEMT-Easy (Promega, USA) and sequenced for verification ...
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bioRxiv - Genetics 2024Quote: ... Effects on endogenous FAS exon 6 inclusion were determined by PCR using GoTaq flexi DNA polymerase (M7806, Promega) and the following primers:
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bioRxiv - Molecular Biology 2024Quote: Cells were seeded in 12-well plates at a density of 200,000 per well and transfected using 1.5 µl/well FuGENE 6 (Promega) with 5 ng of CMV-Renilla plasmid (pTG888) ...
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bioRxiv - Molecular Biology 2024Quote: ... each well was transfected with 1 μg of plasmid DNA (0.5 μg L1 plasmid + 0.5 μg pCEP/GFP) using 4 μL of FuGENE 6 transfection reagent (Promega); the following day ...
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bioRxiv - Cell Biology 2024Quote: ... precipitated proteins were resuspended in 0.1% RapiGest with 6 μL of sequencing-grade trypsin (Promega, 0.5 μg/μL) added to each sample and digested overnight at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 µl RNasin Plus (Promega, catalog no. N2615), 0.8 µl 100 mM ATP (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... using 2 µg of sequencing grade trypsin (Promega) per fraction 55 ...
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bioRxiv - Biochemistry 2022Quote: ... either 2 µg pGL4.47[luc2P/SIE/Hygro] (Promega) or pGL4.52[luc2P/STAT5RE/Hygro] (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... t-ERK1/2 (Promega V114A, 1/1000 dilution), p-AKT (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2021Quote: ... 2) pRL Renilla luciferase control reporter (Promega, E2241) and 3 ...
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bioRxiv - Cancer Biology 2021Quote: ... pGL4.10 (Luc.2; E665A) was purchased from Promega. The pGL4.10-ITGB1 reporter and a control Renilla luciferase vector were co-transfected into breast cancer cell lines using Lipofectamine 3000 transfection kit (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... plus 2 uL of random primers (Promega, C1181). This was then incubated at 70 °C for 10 mins ...
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bioRxiv - Systems Biology 2023Quote: ... 2 µl of 5x MTase-Glo Reagent (Promega) was added to wells ...
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bioRxiv - Cancer Biology 2023Quote: ... we used CellTiter Glo 2 assay systems (Promega). Approximately 2.0 × 103 cells/well were seeded in a 96-well plate and incubated in RPMI1640 culture medium over 4 days at 37 °C and 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... 2 µL of 100 bp DNA Ladder (Promega) was added to confirm amplicons size and run at 100 V for 45 mins ...