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Citations for Promega :
951 - 1000 of 1782 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... lysyl endopeptidase (Wako) at room temperature for 4 hrs and were further digested overnight with 1:50 (w/w) trypsin (Promega) at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and Bone Morphogenetic Protein 4 (BMP4, 10 ng/ml, Bio-Techne) plus phosphoinositide 3-kinase inhibitor Ly294002 (10 µM, Promega). After 42 h incubation at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were resolved in reducing and non-reducing SDS-PAGE gels and membranes were incubated overnight at 4°C with the following antibodies: rabbit polyclonal anti-human p75 intracellular domain (1:1000, Promega); mouse monoclonal anti-HA (1:2000 ...
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bioRxiv - Microbiology 2022Quote: ... The resultant plasmid and Bsu36I-digested BmNPV-abb genome DNA [47] were cotransfected into BmN-4 cells using FuGeneHD (Promega). The recombinant virus was purified twice using the plaque assay by identifying OB-producing plaques ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were then diluted to 4 M urea with 50 mM Tris-HCl pH 8 and Trypsin/Lys-C mix (Promega) was added to an enzyme:substrate ratio of 1:250 (w/w ...
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bioRxiv - Microbiology 2020Quote: ... Protein digestion was performed using 1:100 (w/w) LysC (Wako Chemicals) for 4 h at 37°C and then finalised with 1:50 (w/w) trypsin (Promega) overnight at 37°C ...
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bioRxiv - Cell Biology 2020Quote: IC50 was determined on days 3 and 4 after vemurafenib treatment using Real-time-Glo MT Cell Viability Assay according to the manufacturer’s protocol (Promega, #G9711). Using a 96-well white plate ...
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bioRxiv - Molecular Biology 2020Quote: ... Apoptosis was assessed by incubating the cells with 200nM Staurosporin or medium for 4 h and the caspase 3/7 activity was assayed using the ApoOne Caspase 3/7 Assay (Promega). Senescence associated β-Galactosidase activity was analyzed with the Senescence Associated β-Galactosidase Staining Kit (Cell Signalling Technologies) ...
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bioRxiv - Plant Biology 2021Quote: ... Treatment with Proteinase K was carried out in 500 μl of PK Buffer (100 mM Tris-HCl pH8; 50 mM NaCl; 10 mM EDTA; Proteinase K 4 mg/ml; RNasin PROMEGA) 2 h at 55 °C and 15 min at 95 °C ...
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bioRxiv - Plant Biology 2021Quote: ... Three washes were performed with ChIP Dilution Buffer as in DRIP assays before incubating the IP fraction with Proteinase K for 2 h at 55°C plus 15 min at 95°C in PK Buffer (100 mM Tris-HCl pH8; 50 mM NaCl; 10 mM EDTA; 4 mg/ml Proteinase K; RNasin PROMEGA). We added 2 U DNase I (ThermoFisher ...
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bioRxiv - Cancer Biology 2020Quote: Selected protein spots were excised from the gel and enzymatically digested in-gel essentially as previously described(4) using trypsin (Promega). Gel pieces were washed with 50% acetonitrile to remove SDS ...
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bioRxiv - Cancer Biology 2021Quote: ... assessed every day for 4 days by measuring ATP content through Cell Titer-Glo® Luminescent Cell Viability assay (Promega), was compared to cell viability assayed at day 1 ...
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bioRxiv - Immunology 2020Quote: ... Single CD3-CD8-CD16- CD20+Ova-RBD-PE+RBD-AF647+ B cells were sorted into individual wells of a 96-well plates containing 4 μl of lysis buffer (0.5 X PBS, 10mM DTT, 3000 units/mL RNasin Ribonuclease Inhibitors (Promega, N2615) per well using a FACS Aria III (Becton Dickinson) ...
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bioRxiv - Neuroscience 2021Quote: ... The transfected cells were then treated with Aβ42 (1-4 μM) or calcitriol (100 nM) for 6 h before luciferase activity assay (Dual-Luciferase Reporter Assay, Promega). A 587-bp region of the human Cyp24a1 gene promoter that contains two VDRE motifs was cloned into the promoter-less luciferase expression vector pGL3-basic (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... peptides were diluted 4-fold with ABC buffer (Ammonium bicarbonate 50 mM pH8) and digested with Trypsin MS grade (Promega) overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... Oligos were then ligated at 4°C overnight to the appropriate digested ACR-16-REM sequence (T4 ligase, Promega, USA) and transformed into DH5α (ThermoScientific ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were passaged every 3–4 days and regularly checked for mycoplasma infections using a GoTaq G2 Hot Start Taq Polymerase kit from Promega.
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bioRxiv - Genetics 2023Quote: ... carrying the non-risk or risk allele of each SNP (Supplemental Table 4) were cloned into the no-promoter firefly luciferase reporter plasmid pGL4.14 [luc2/Hygro] (Promega, #E6691) or minimal promoter firefly luciferase reporter plasmid pGL4.26 [luc2/miniP/Hygro] (Promega ...
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bioRxiv - Microbiology 2024Quote: ... In vitro mRNA synthesis was then carried out at 37 °C for 4 h in T7 RiboMAX Large Scale RNA Production System kit (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... For in vitro import assay WT and MLS-TFEB cDNA were cloned into the pGEM-4 vector and transcribed in vitro using the TNT Quick coupled translation system (L1170, Promega), by incubating 200 ng of DNA with 7 μL of TNT-T7 reticulocyte lysate mix and 0.5 µL of 35S-labelled methionine (NEG709A500UC ...
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bioRxiv - Immunology 2023Quote: ... total RNA was purified from 3-4 tissues of female flies using a ReliaPrep RNA Tissue Miniprep kit (Promega, z6112). Quantitative RT-PCR was performed using a OneTaq RT‒PCR kit (Promega ...
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bioRxiv - Neuroscience 2023Quote: ... the washed beads were re-suspended in 150 µl trypsin digestion buffer and incubated for 4 hours with 1 µg trypsin (Promega) at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... S2R+ cells were grown in SF-4 medium and cotransfected with desired plasmids using FuGENE® HD transfection reagent (Promega). Specifically ...
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bioRxiv - Biochemistry 2023Quote: ... Selected gel bands that did not bind to the 4’-methylgenistein beads were subjected to in-gel digestion with trypsin (MS grade; Promega). Peptide fragments were analyzed using a Q-TOF/MS ...
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bioRxiv - Plant Biology 2023Quote: ... Protein for the 4-methylumbelliferyl-b-D-glucuronide (MUG) and luciferase (LUC) assays was extracted in 1x passive lysis buffer (PLB: Promega). MUG assays were performed by adding 40 mL of protein extract to 100 mL of MUG assay buffer [2 mM MUG ...
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bioRxiv - Cell Biology 2023Quote: ... The antibodies used for western blotting were: anti-HALO (1:1000 in 0.5% milk TBST, 4°C o/n, Promega G9211), anti-GAPDH (1:10000 in TBST ...
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bioRxiv - Cancer Biology 2023Quote: ... Target and effector cells were cultured 4 hours or overnight and cytotoxicity was measured using the CytoTox96 LDH assay (Promega) or using click beetle green luciferase expressing cells and read on a BioTek Synergy H1 plate reader (BioTek Instruments) ...
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bioRxiv - Developmental Biology 2024Quote: ... The 4 µg of linearized vectors were transcribed 5h using RiboMAX™ Large Scale T7 RNA polymerase kit (Promega, #P1300) with 5.6 mM Ribo m7G Cap Analog (Promega ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were transduced with the shRNA lentiviruses and selected on puromycin for 4 days before lysis in 1× passive lysis buffer (E1941, Promega) and dual luciferase readout using the Dual-Glo® Luciferase Assay System (E2940 ...
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bioRxiv - Cell Biology 2024Quote: ... then transfected with 4 μg pLenti-CMV-PuroDEST-ERK-KTR-mClover DNA plus 12 μL FuGENE 6 (Promega Cat. #E2691) in 800 μL of Opti-MEM (Thermo-Fisher Cat ...
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bioRxiv - Bioengineering 2024Quote: ... and incubated with the following primary antibodies diluted in TBS-T overnight at 4°C: mouse anti-HaloTag (1:5,000; Promega, G9211), mouse anti-Flag M2 (1:2000 ...
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bioRxiv - Cancer Biology 2024Quote: ... and then incubated overnight at 4°C with mouse anti-HiBiT antibody (Promega #N7200; 1 µg/mL; diluted 1:1000) in 1X PBS containing 5% BSA ...
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bioRxiv - Microbiology 2024Quote: ... cells were washed in PBS, collected by centrifugation (2000 rpm, 10 min, 4°C) and lysed in reporter lysis buffer (Promega). Lysates were spotted to 96-well dishes ...
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bioRxiv - Microbiology 2024Quote: ... In vitro mRNA synthesis was then carried out at 37 °C for 4 h in T7 RiboMAX Large Scale RNA Production System kit (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2024Quote: ... the procedure was the same except that protein extract samples were digested with Lyc-C (Lysyl Endopeptidase, WAKO) for 4 h and trypsin (Promega) overnight ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 1□µg of plasmid DNA (pIZ/V5-His bearing either Hmmasc or Hm-oscar and non-inserted vector as negative control) was transfected to the BmN-4 cells using FuGENE HD (Promega). Three days after transfection ...
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bioRxiv - Bioengineering 2024Quote: ... control and printed aggregates were collected for 4 hours and 3 days post-printing and tested following the CellTiter96 (Promega) assay protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Plates were then incubated overnight at 4°C with primary antibodies (mouse anti-LgBIT antibody at 1:750 dilution [Promega N7100] ...
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bioRxiv - Biophysics 2024Quote: ... Primary antibodies were diluted in the same buffer and added to the cells ON at 4°C (mouse anti-HiBiT (Promega) catalog number CS2006A01 clone 30E5 ...
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bioRxiv - Cell Biology 2024Quote: ... protein samples were then incubated with chymotrypsin at a ratio of 1:80 (enzyme to protein) for 3-4 h at RT and then trypsin (Promega) at a ratio of 1:80 (enzyme to protein ...
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bioRxiv - Biochemistry 2024Quote: 20merRBNS and posRBNS DNA pool was synthesized by IDT (Supp. Table 4) and was in vitro transcribed using T7 RiboMAX Express large scale RNA kit (Promega) according to manufacturer protocols.
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bioRxiv - Genetics 2020Quote: ... 2.4 µl of RQ1 DNase Buffer and 2 µl of RQ1 RNase-Free DNase (M6101, Promega) was added to each RNA sample and incubated for 15 minutes at 37°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... cDNA was synthesized from 2 μL of the eluted RNA using M-MLV reverse transcriptase (Promega) and random hexamer primers ...
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bioRxiv - Developmental Biology 2021Quote: ... and the resulting mRNAs (2 μg) were translated in 50 μl of rabbit reticulocyte lysate (Promega). Flag-tagged proteins were purified by immunoprecipitation with anti-Flag M2 antibody (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... Viral RNA of SARS-COV-2 was detected by TaqMan®-based Real-Time PCR (Promega) using a CDC protocol with two sets of primer/probe which amplifies virus nucleocapsid (N ...
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bioRxiv - Molecular Biology 2021Quote: ... thiopropyl sepharose beads were incubated overnight at 37°C with 2 μg trypsin Gold (Promega, #V5280) in 50 mM NH4HCO3 ...
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bioRxiv - Genetics 2021Quote: ... The purified DNA was PCR amplified using 2× GoTaq Colorless Master Mix (Promega, Madison, WI, USA), and PCR product was extracted as above to eliminate primer-dimers ...
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bioRxiv - Plant Biology 2021Quote: ... 8 µL of a mix consisting of 2 µL deoxyribonucleotide triphosphate (dNTPs, Promega, Madison, Wisconsin, US), 4 µL 5x RT-buffer ...
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bioRxiv - Plant Biology 2020Quote: ... were digested for 12 hr at 37 °C with 2 mg of sequencing-grade trypsin (Promega), followed by a second incubation for 6 hr with an additional 2 mg of trypsin ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized from 2 μg of total RNA with the MMLV kit (Promega, Madison, WI). Semi-quantitative PCR was performed within the linear amplification phase using a recombinant Taq Polymerase (Invitrogen ...