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Citations for Promega :
901 - 950 of 1782 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: cDNA was synthesized from 2 ug of total RNA using GoScript Reverse Transcriptase (Promega, #A5003) with random hexamers according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... The mice received intraperitoneal (IP) injection of 2 milligrams of luciferin (Promega Corporation, Madison, WI) and subjected to luminescence imaging using an IVIS® Spectrum In Vivo Imaging System (PerkinElmer Inc ...
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bioRxiv - Biochemistry 2024Quote: ... pH 8.0) containing 2 nM 11S and 1/250 Nano-Glo Luciferase Assay Reagent (Promega) was added to the cells and background luminescence read for 5 min ...
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bioRxiv - Biophysics 2024Quote: ... cells were transiently transfected with 2 µg DNA by lipofection (FuGENEⓇ HD, Promega, Madison, WI).
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bioRxiv - Neuroscience 2024Quote: ... The brain surface was covered in 2-3% low melting point agarose (Promega, Madison, WI) in sterile saline and then capped with silicone elastomer ...
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bioRxiv - Bioengineering 2024Quote: ... and codon optimized human PAH exon 2-13 coding sequence with HiBit reporter tag (Promega) and cloned into plasmid backbone with Gibson assembly ...
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bioRxiv - Cancer Biology 2024Quote: ... Tryptic digest was performed in 50 mM ammonium bicarbonate buffer with 2 μg trypsin (Promega) at 37°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... 5 % CO2 for 72 h before being washed with PBS and 200 µL Passive Lysis buffer (Promega, UK) added to each well ...
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bioRxiv - Genomics 2020Quote: ... PCR amplification was performed in a 10- μL reaction mixture containing 5 μL of GoTaq Master Mix (Promega), 5 pmol FAM-labeled universal primer (5′ - FAM-gctacggactgacctcggac-3′) ...
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bioRxiv - Biochemistry 2020Quote: ... After the addition of 5 mM ATP the reaction was taken to a plate reader (Promega GloMax Navigator) and the luminescence collected for approximately 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... entire gel lanes were excised into 5 equal regions from top to bottom and digested with trypsin (Promega) as described (30) ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was diluted 1:5 before quantitative analysis by qPCR was done using GoTaq qPCR Master Mix (Promega) following manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... Ten nanograms of template DNA was added to 5 µl Green GoTaq Reaction Mix Buffer (Promega, Madison, WI), 1.25 units GoTaq DNA Polymerase (Promega ...
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bioRxiv - Cancer Biology 2022Quote: ... pMD2.G and the lentiviral gRNA plasmid at a 3:1:5 mass ratio using FuGENE HD (Promega) in Opti-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... 8 μl 5x PNK pH 6.5 buffer [350 mM Tris-HCl pH 6.5, 50 mM MgCl2, 5 mM DTT], 1 μl PNK enzyme [NEB], 0.5 μl RNasin [Promega] ...
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bioRxiv - Plant Biology 2021Quote: ... The powder was resuspended in 30 mL of Extraction Buffer 1 (10mM Tris-HCl pH8; 0.4M sucrose; 10 mM MgCl2; 5 mM BME; 0.2 mM PMSF; RNasin PROMEGA) and was filter thought a Nylon membrane of 150 μm and centrifuged at 2000 g for 20 min at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... zyxin(-/-) MEFs were transfected with 5 μg total DNA using the Fugene® 6 transfection reagent (E2691, Promega) and plated on glass coverslips.
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bioRxiv - Immunology 2020Quote: ... Cell death after 5 h treatment was quantified by PI incorporation or using the Caspase Glo system (Promega) according to manufacturer instructions.
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bioRxiv - Immunology 2021Quote: ... and HHEX (NM_002729) attached to a FLAG tag at the 5’ end and HiBit tag (Promega, Madison, WI) at the 3’ end ...
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bioRxiv - Cell Biology 2021Quote: The Rosa26 promoter and the 5’UTRs of secretory granule protein transcripts were subcloned into the pNL1.1 (Promega) using standard cloning techniques ...
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bioRxiv - Molecular Biology 2023Quote: ... 1M Tris-HCl was added to adjust the pH and 5 μL trypsin (2.5 ug, Promega Sequence Grade) was added and the samples digested overnight at 37℃ ...
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bioRxiv - Neuroscience 2023Quote: ... DA at concentrations ranging from 0.01 nM to 100 μM and 5 μM furimazine (NanoLuc Luciferase Assay, Promega) were then bath-applied to the cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was assessed 5 days after interferon-β treatment with CellTiter-Glo Luminescent Cell Viability Assay (Promega). ATP bioluminescence values of the interferon-β treated wells were normalized to those of the vehicle-treated controls ...
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bioRxiv - Cell Biology 2023Quote: ... 5 μL culture were added to 45 μL NanoGlo reagent (1x Promega lysis buffer and NanoGlo reagent; Promega). The average luminescence of the background cultures was subtracted to the average luminescence of each sample.
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bioRxiv - Microbiology 2022Quote: ... reverse transcription was performed with 0.5 μl (corresponding to 5 U) of Avian Myeloblastosis Virus Reverse Transcriptase (Promega), 0.1 μl dNTP mix (10 mM) ...
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bioRxiv - Biophysics 2022Quote: ... The poly-L-lysine coated side of the cover slip was exposed to 5 mg/ml streptavidin (Promega) in this space for 15 min ...
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bioRxiv - Microbiology 2022Quote: ... Then reaction was stopped by the addition of 5.5 μL of a mix containing 5 % SDS and 0.25 M EDTA and deproteinized with proteinase K (Promega) for 1 hour at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were then concentrated down to 20 µL and supplemented with 5 µg of sequencing grade trypsin (Promega) in 15% ACN in 200 mM aqueous TEAB ...
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bioRxiv - Cancer Biology 2023Quote: ... Subsequent measurements were taken every 24 hrs for 5 days using CellTiterGlo Luminescent Cell Viability Assay kit (Promega) and SpectraMax plate reader (Molecular Devices ...
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bioRxiv - Microbiology 2024Quote: ... reverse transcription reactions were performed by adding 0.5 µl (corresponding to 5 U) of AMV Reverse Transcriptase (Promega), 0.1 µl dNTP mix (10 mM) ...
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bioRxiv - Cancer Biology 2024Quote: ... lactate assay was performed using 5 µl of media and the luminometric Lactate-Glo assay kit (Promega, #J5021) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Ten nanograms of template DNA was added to 5 µL Green GoTaq Reaction Mix Buffer (Promega, Madison, WI), 1.25 units of GoTaq DNA Polymerase (Promega ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were then transferred to opaque 96-well plates containing 5 μM furimazine (NanoLuc Luciferase Assay, Promega) and bathed in ACh at concentrations ranging from 0.1 nM to 100 μM ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 μL of a mixture of 10 mM dNTPs and 200 U of M-MLV reverse-transcriptase (Promega) were added to the mixture and incubated at 37 °C for 60 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... shaken vigorously and sonicated in water bath for 5 min before 50 µL of 0.2% ProteaseMAX (Promega, USA) in 10% acetonitrile (ACN ...
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bioRxiv - Genetics 2024Quote: ... Exon 5 and exon 17 of PNPT1 were amplified by PCR using GoTaq® Green Master Mix (Promega) and the primers 5a - 5b for exon 5 and 17a - 17b for exon 17 ...
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bioRxiv - Biochemistry 2021Quote: ... The residual magnetic beads were then incubated for two hours at 4°C and for one hour at 16°C in 83 microlitres of buffer A200 with 20 Units RNasin (Promega), 12mM DTT and 16 micrograms of TEV-Protease ...
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bioRxiv - Genomics 2021Quote: ... RNA concentration was measured in a DeNovix DS-11 spectrophotometer (DeNovix Inc, USA) and 4 μg of RNA were subjected to DNAse treatment (RQ1 - Promega) according manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: Plasmid DNAs containing Tol2 vectors comprising GV genes and fluorescent protein genes described above and transposase mRNA were introduced to KPL-4 cells by lipofection using FuGene HD (Promega) in OptiMEM (Gibco) ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were digested with 0.5 µg of lysyl endopeptidase (Wako) at room temperature for 4 hours and were further digested overnight with 1 µg trypsin (Promega) at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were incubated at 37°C for 1 hour and then proteolytic digestion was performed with LysC (Wako) for 4 hours and trypsin (Promega) overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... The sequences ligated by the T4 ligase at 4 °C for 12 h were cloned in the pGEM T-easy vector (Promega). The competent Escherichia coli X10-Gold (Stratagene ...
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bioRxiv - Cell Biology 2021Quote: HeLa cells cultured in 24-well plates were transfected with indicated firefly luciferase reporter construct (SBE×4-luc or BRE-luc) together with pRL-SV40 renilla luciferase control vector (Promega) using Lipofectamine 2000 (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Twenty nanograms of DNA were used as a model for 18 cycles of amplification of the V4 region of the 16S rRNA gene, using primers 515F and 806R (Caporaso, et al. 2012: 1621-4) and GoTaq Master Mix (Promega). PCR products were quantified with the Qschd dsDNA HS kit (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... After 30 min at 60°C the samples were cooled to room temperature and 4 μg of Sequencing Grade Trypsin (Promega) for digestion were added ...
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bioRxiv - Cell Biology 2021Quote: ... Washed beads were re- suspended in 150 µl digestion buffer and incubated for 4 hours with 1 µg trypsin (Promega) at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... 8 min, 4 °C) and resuspended in staining buffer (PBS, pH 7.4, with 0.5% RNase-free BSA (Promega, Heidelberg, Germany) and 0.2 U/μl of RNasin Plus RNase inhibitor (Promega)) ...
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bioRxiv - Genomics 2022Quote: ... The pRF+423Dux 4 vector was sequenced using primers that anneal to the pGL3 vector (Promega, see primers Table S2). The Dux4 IRES site was deleted from pRF+423Dux4 using PCR primers that flank the IRES region (PRF423DUX4-ATW F and R ...
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bioRxiv - Cancer Biology 2022Quote: ... 25,000 leukemia cells in 250µL of drug-containing medium were seeded in a 48-well plate and viability was assessed every 4 days by using the CellTiter-Glo Luminescent Cell Viability assay (Promega). For RNA-Seq and ChIP-Seq experiments ...
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bioRxiv - Biochemistry 2022Quote: ... Gel pieces were then rehydrated in 50 ul 50 mM ammonium bicarbonate containing 4 ng/µl trypsin (Promega, Madison, WI) and 0.01% proteaseMAX (Promega ...