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Citations for Promega :
51 - 100 of 5086 citations for Human Guanylate Binding Protein 6 GBP6 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... The protein concentration was estimated using a BCA kit (Promega). The blots were blocked using 5% NFDM (Santa Cruz ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined using Bradford Assay Kit (Promega, USA) and equal amounts of proteins were denatured ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein samples were re-solubilized in 6 M urea buffer followed by reduction and alkylation before digestion with trypsin (Promega) at 37°C for 18 hr ...
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bioRxiv - Biochemistry 2024Quote: ... at a 1:75 enzyme to protein ratio for 6 h at 30 °C and then by trypsin (V5111, Promega) (1:50 enzyme to protein ratio ...
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bioRxiv - Microbiology 2020Quote: ... or Vpr F34I/P35N mutant using 6 μl Fugene-6 (Promega). For KPNA-cargo IPs HEK293T cells were grown in 10 cm dishes and co-transfected with 1 μg of a plasmid expressing FLAG-tagged KPNA1 ...
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bioRxiv - Immunology 2022Quote: ... Nonspecific binding was blocked with PBS+ 0.5% Tween® 20 (Promega) + 10% FBS for 2 hours at RT ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... 24h prior to imaging cells were co-transfected with human PEX31-42-GFP-Halo and either human HAP1-mCherry-eDHFR or mouse BICD21-572-mCherry-eDHFR using FuGENE 6 (Promega; 1 µg total DNA) and 48h prior to imaging with control siRNA using Lipofectamine RNAiMAX (Thermo Fisher) ...
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bioRxiv - Cell Biology 2022Quote: ... Baseline fluorescence was measured in an ELISA reader (Promega) following an incubation of 30 min at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... Human β2AR was fused at its N-terminus to the ACP- or Halo7-tag protein (NEB and Promega, respectively) at the cDNA level ...
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bioRxiv - Microbiology 2020Quote: ... HeLa cells were transfected 6 hours before imaging with Fugene 6 (Promega) and 1000ng of DNA ...
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bioRxiv - Cell Biology 2020Quote: ... gel regions were diced and proteins were subjected to in-gel digestion with 6 ng/μL trypsin (Promega, Madison, WI, USA) in 50 mM ammonium bicarbonate at 37 °C for 10-12 hours ...
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bioRxiv - Cell Biology 2020Quote: ... using Fugene 6 (Promega). Supernatant was harvested from the transfected cells for the next three days and then centrifuged ...
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bioRxiv - Cell Biology 2020Quote: ... using Fugene 6 (Promega), with a linearized TCIS construct described previously6 ...
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bioRxiv - Genetics 2021Quote: ... using FuGENE 6 (Promega) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... using FuGENE 6 (Promega). Sixteen hours later ...
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bioRxiv - Cancer Biology 2020Quote: Transfection: Fugene 6 (Promega); TransIT-2020 (Mirus Bio) ...
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bioRxiv - Biophysics 2020Quote: ... FuGENE 6 (Promega, E2691) was used for transient transfection according to the manufacturer’s instructions.
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bioRxiv - Genetics 2019Quote: ... using FuGENE 6 (Promega) on day 0 ...
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bioRxiv - Developmental Biology 2022Quote: ... or Fugene 6 (Promega) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... using FuGENE 6 (Promega). Cells were cultured overnight at 37°C with 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... using Fugene 6 (Promega).
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bioRxiv - Microbiology 2020Quote: ... using FuGENE 6 (Promega). Sixteen hours later ...
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bioRxiv - Cell Biology 2022Quote: ... or FuGENE 6 (Promega) following the manufacturer’s protocol with modifications (see below).
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bioRxiv - Microbiology 2022Quote: ... or Fugene 6 (Promega) and supernatants harvested 3 days later ...
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bioRxiv - Cell Biology 2023Quote: ... utilizing Fugene 6 (Promega). Culture supernatants were collected at 48h and 72h post-transfection ...
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bioRxiv - Genomics 2023Quote: ... Protein was precipitated by the addition of 200 μl Protein Precipitation Solution (Promega Wizard HMW DNA Extraction Kit A2920). Using a wide bore tip the samples were mixed by drawing up contents from the bottom of the tube and then expelled on the side of the tube 5 times ...
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bioRxiv - Cancer Biology 2020Quote: ... Human cell lines were DNA fingerprinted for provenance using the Power-Plex 1.2 kit (Promega) and confirmed to be the same as the DNA fingerprint library maintained by ATCC ...
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bioRxiv - Developmental Biology 2024Quote: ... Total peptide concentration was adjusted to 0.15 µg/µL according to the absorbance at 280 nm against a Mass Spec-Compatible Human Protein Extract/Digest calibration curve (Promega), using the Thermo Nanodrop 1000 ND-1000 spectrophotometer (Thermo Fisher).
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bioRxiv - Molecular Biology 2019Quote: ... Plasmid DNA (6 μg) was delivered into U2OS cells using FuGENE 6 (Promega) and 2.5 μg of plasmid DNA was delivered into HeLa cells using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... 6 uL of RNA was used for each cDNA synthesis (GoScript™ Reverse Transcription System kit, Promega). qPCR was performed using Fast SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: PKA activity (PepTag® Non-Radioactive Protein Kinase Assay kit V5340, Promega) was carried out according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HiBit-tagged proteins were detected using the NanoGlo HiBit Blotting kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... and 1,750 ng landing pad G384A vector template using 6 μL Fugene 6 (Promega) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... based on a mutated RIIB cAMP-binding domain from PKA (pGloSensor-20F, Promega), which produces rapid and reversible cAMP-dependent activation of luciferase activity in intact cells and is capable of detecting cAMP elevations in the absence of phosphodiesterase inhibitors ...
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bioRxiv - Cancer Biology 2023Quote: ... and E2 DNA-binding sites) and to pSV40-luc (pGL3-Control from Promega, containing the SV40 promoter and enhancer regions but no E2 DNA-binding sites) ...
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bioRxiv - Molecular Biology 2020Quote: Human Genomic DNA was extracted from the blood using the Wizard Genomic DNA Purification kit (Promega) as per the instructions.
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bioRxiv - Neuroscience 2021Quote: Genomic DNA (gDNA) was extracted from human frontal cortex using Wizard Genomic DNA Purification Kit (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... approximately 0.6 mg IgG-enriched proteins were first digested on the beads with mass spectrometry-grade Trypsin/Lys- C Mix (Promega, Madison, WI, USA) at a 1:50 (enzyme/substrate ...
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bioRxiv - Cell Biology 2021Quote: ... or Fugene 6 (Promega #E2691) for 5 hours ...
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bioRxiv - Cell Biology 2020Quote: ... FuGENE 6 Transfection Reagent (Promega) was used according to the manufacturer’s protocol and the phenotype inspected 24 or 48 h after transfection ...
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bioRxiv - Genomics 2020Quote: ... using Fugene 6 (Promega, E2691). Virus was then harvested and applied to Scc1-EGFP-AID/dCas9-KRAB cells ...
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bioRxiv - Neuroscience 2020Quote: ... using FuGENE®-6 (Promega). Supernatants containing viral particles were aseptically collected 3 days post-transfection ...
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bioRxiv - Cell Biology 2021Quote: ... using FuGENE 6 (Promega; E2691). Lentiviral particles were harvested and concentrated using a Lenti-X Concentrator (Clontech ...
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bioRxiv - Microbiology 2021Quote: ... using Fugene 6 (Promega # E2691), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... using Fugene 6 (Promega #E2691) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... Trypsin (6 ng/ml, Promega) in NH4HCO3 (50 mM ...
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bioRxiv - Microbiology 2021Quote: ... using FuGENE 6 (Promega, # E2311). 40 hours post-transfection ...
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bioRxiv - Cancer Biology 2019Quote: ... FuGENE 6 Transfection Reagent (Promega) was used for DNA transfection ...
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bioRxiv - Microbiology 2020Quote: ... or Fugene 6 (Promega, UK) and virus containing supernatants harvested 3 days later ...
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bioRxiv - Neuroscience 2021Quote: ... or Fugene® 6 (Promega) in a 3:1 ratio with cDNA mix ...