Labshake search
Citations for Promega :
901 - 950 of 4999 citations for 6 Bromo 3 3H imidazol 4 ylmethylene 1 3 dihydro indol 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... CDC was measured after incubation for 3 hours at 37 °C 5% CO2 with a luminometer using the CytoTox-Glo Cytotoxicity Assay (Promega; Cat. Nr.: G9291) according to the manufacturer’s instructions.
-
bioRxiv - Microbiology 2023Quote: ... The copy numbers of the control DNA/RNA were calculated based on the molecular weight and absorbance at 260 nm of RNA that had been serially diluted (≥3 steps) and/or fluorescence using the Quantus Fluorometer (Promega, Madison, WI, USA) (n = 3).
-
Development of a genetically-encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2023Quote: HEK293 cells growing at 70% confluency in a 10 cm dish were transfected with wild-type human NOPR or NOPLight (3 µg DNA) and GloSensor-20F (Promega, 2.5 µg DNA) using 12 µL Lipofectamine 2000 (Thermo Fisher ...
-
bioRxiv - Microbiology 2023Quote: ... After five-fold dilution with 50-mM ammonium bicarbonate, the sample was digested using Lys-C (Wako, Osaka, Japan) at 37°C for 3 h following trypsin (Promega, Madison, WI, USA) at 37°C for 16 h ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... membrane integrity (CytoTox-OneTM homogeneous membrane integrity assay kit: lactate dehydrogenase (LDH release) and caspase activity/ apoptosis (Caspase-Glo® 3/7 assay system kit) (all from Promega, WI, USA), as described previously17 (see Supplementary data for detailed description).
-
bioRxiv - Evolutionary Biology 2023Quote: ... The amplicons generated from the 5’ and 3’ race PCRs were subjected to purification and subsequent cloning into the pGEM-T easy vector (Promega Corp., WI, USA). The constructed vectors were sequenced (Plasmidsaurus ...
-
bioRxiv - Bioengineering 2024Quote: ... 100 uL of the diluted lysate was added to each well along with an equal volume of Caspase Glo 3/7 assay reagent (Promega, Madison, Wisconsin, USA), according to the manufacturer’s directions ...
-
bioRxiv - Biochemistry 2021Quote: ... 1 mM CaCl 2 and 10 ng/μL trypsin (Promega). Trypsin digestion was performed at 37 °C with rotation overnight and the beads were washed three times with PBS and distilled water again ...
-
bioRxiv - Cell Biology 2019Quote: ... parental HeLa cells were transfected with 2 μg plasmid containing a GFP cassette and 4 μl FuGENE HD (Promega) in a six-well cluster ...
-
bioRxiv - Molecular Biology 2023Quote: ... at 10,000 x g and 4°C for 2 min and digested by addition of RNAse-free DNAse I (Promega) for 10 min on ice ...
-
bioRxiv - Immunology 2022Quote: ... Pelleted cells were incubated with ONE-Glo reagent for 30 minutes at room temperature following the ONE-Glo Luciferase Assay System protocol from Promega (Catalog # E6130). Luminescence was quantified by the Synergy H1 Hybrid reader (BioTek ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... using the CytoTox-ONE Homogeneous Membrane Integrity assay according to the manufacturer’s instructions (CytoTox-ONE Homogeneous Membrane Integrity Assay; #G7891, Promega, Madison, United States). 50 µl medium from each well was removed ...
-
bioRxiv - Microbiology 2021Quote: ... cDNA was synthesized using 5 μl of the eluent as template using the U3 antisense primer 5’ TGTGTGGTAGATCCACATCG-3’ and M-MLV RT RNase [H-] (Promega, WI. Cat. No. MR3681) following the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2020Quote: ... The cells were then co-transfected with either 0.3 μg/well of Smad4 firefly luciferase reporter plasmid constructs (pLuc366 or pLuc207) or the control pGL3-Basic vector (Promega, San Luis Obispo, USA). The renilla luciferase plasmid was also co-transfected to correct for variations in transfection efficiency (45 ng/well) ...
-
bioRxiv - Molecular Biology 2022Quote: ... the cells were transfected with 1 μg of DNA (1 μg of an L1-expressing plasmid or 0.5 μg of the L1-expressing plasmid and 0.5 μg of either a pCMV-3Tag-8-Barr control or ISG-expressing plasmid) using 3 µL of FuGENE HD transfection reagent (Promega, Madison, WI, United States) and 100 µL of Opti-MEM (Gibco ...
-
bioRxiv - Neuroscience 2022Quote: ... the pHAD plasmid was linearized by PmeI enzyme to expose the ends of the 5’ and 3’ inverted terminal repeats (ITRs) and transfected into 116 producer cells (Profection® Mammalian Transfection System, Promega, Madison, WI, USA). Helper virus (HV ...
-
ADEVO: Proof-of-concept of Adenovirus Directed EVOlution by random peptide display on the fiber knobbioRxiv - Bioengineering 2023Quote: ... Media was changed at 3 hpi and luciferase luminescence was measured at 24 hpi using the Nano-Glo® Luciferase Assay (Promega, Madison, USA, #N1130) kit ...
-
bioRxiv - Molecular Biology 2021Quote: ... 5 µl of the reaction was treated with 1 µl of RNase mixture (10% RNase A, Sigma + 20% RNase One, Promega), incubated at 37°C for 10 min and then mixed with 6 µl of 2 × SDS-PAGE buffer ...
-
bioRxiv - Cell Biology 2020Quote: ... using one of the following antibodies against (all dilutions in PBS): HaloTag® polyclonal anti-rabbit (G928A, Promega, 1:100); ACTN2 monoclonal anti-mouse (EA-53 Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2022Quote: ... were transfected using FuGene 6 (Promega). The supernatant containing V0 virus (virus passage zero (progeny virus) ...
-
bioRxiv - Immunology 2022Quote: ... using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... using FuGene 6 transfection reagent (Promega). The medium was changed 18 h after transfection ...
-
bioRxiv - Cell Biology 2021Quote: ... and 1.2 µl Fugene 6 (Promega) transfection mix and incubated for 15min at room temperature before adding to HeLa cells plated in one well of a 24 well plate ...
-
bioRxiv - Cell Biology 2021Quote: ... FuGENE 6 reagent (Promega, Madison, WI) was used for transient transfection of HeLa cells according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... S7A–C using FuGene 6 (Promega). Two days after transfection ...
-
bioRxiv - Molecular Biology 2019Quote: ... 8μL of Fugene-6 (Promega, E2691) was mixed with various amounts of plasmid DNA and allowed to incubate at room temperature for 15 minutes before addition to cells ...
-
bioRxiv - Cell Biology 2021Quote: ... FuGENE® 6 Transfection Reagent (Promega); Luminata Forte Western HRP substrate (Millipore) ...
-
bioRxiv - Microbiology 2019Quote: ... using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... MDCK) or with FuGENE 6 (ProMega) (HeLa) ...
-
bioRxiv - Cancer Biology 2021Quote: ... using FuGENE 6 Transfection Reagent (Promega) according to manufacturer protocol ...
-
bioRxiv - Genomics 2023Quote: ... and transfected (Fugene 6, Promega # E269A) with pBABE-puro SV40 LT plasmid (Addgene # 13970 ...
-
bioRxiv - Immunology 2023Quote: ... using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ONE-Glo Luciferase assay system was purchased from Promega. HEK-293T ERSE-Luciferase cells ...
-
bioRxiv - Immunology 2021Quote: ... 40 μL/well of One-Glo-EX substrate (Promega) was added to the cells and incubated in the dark for 5-10 min prior to reading on a BioTek plate reader ...
-
bioRxiv - Immunology 2019Quote: ... 5% CO2 CellTiter 96 Aqueous One Solution (Promega, #G3582) was added to measure cell proliferation ...
-
bioRxiv - Bioengineering 2020Quote: ... 20 μL of Cell Titer Aqueous One Solution (Promega) was added to the wells and after incubation of 2 hours at 37°C the absorption at 492 nm was measured.
-
bioRxiv - Cancer Biology 2020Quote: ... CellTiter 96 AQueous One Solution (Promega, Walldorf, United States) was used to determine relative cell proliferation every other day ...
-
bioRxiv - Cell Biology 2021Quote: ... 20μl of CellTiter 96 Aqueous One Solution (G3582, Promega) was dispensed per well ...
-
bioRxiv - Molecular Biology 2020Quote: ... fluorometer using the QuantiFluor ONE dsDNA system (Promega E4871). This system recorded fluorometric readout from each well using an emission filter of 500-550nm ...
-
bioRxiv - Neuroscience 2021Quote: ... 25µL of ONE-Glo Assay Buffer (Promega, cat# E6120) was added to the lysate ...
-
bioRxiv - Immunology 2021Quote: ... 40 μL/well of One-Glo-EX substrate (Promega) was added to the cells and incubated in the dark for 5-10 min prior to reading on a BioTek plate reader ...
-
bioRxiv - Microbiology 2021Quote: ... or CellTiter-Glo® One Solution Assay system (Promega) following manufacturer protocols ...
-
bioRxiv - Immunology 2022Quote: ... 60 μl/well of One-Glo-EX substrate (Promega) were added to the cells and incubated in the dark for 5-10 min prior reading on a Varioskan LUX plate reader (Thermo Fisher) ...
-
bioRxiv - Genetics 2022Quote: ... and quantified using the QuantiFluor ONE dsDNA System (Promega). Genomic DNA was sheared to ∼200 bp fragments with sonication ...
-
bioRxiv - Cancer Biology 2019Quote: ... CellTiter 96 Aqueous One Solution reagent (Promega, Madison, USA) was added to each well ...
-
bioRxiv - Immunology 2021Quote: ... 40 μL/well of One-Glo-EX substrate (Promega) was added to the cells and incubated in the dark for 5-10 min prior reading on a BioTek plate reader ...
-
bioRxiv - Immunology 2021Quote: ... 50 μL/well of One-Glo-EX substrate (Promega) were added to the cells and incubated in the dark for 5-10 min prior reading on a Varioskan LUX plate reader (ThermoFisher) ...
-
bioRxiv - Immunology 2020Quote: ... ONE-Glo EX Luciferase Assay Substrate (Promega, Cat. E8110) was added to cells and incubated for 3 minutes at room temperature with shaking ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... the Cell Titer Glo One solution kit from Promega was used ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... the Cell Titer Glo One solution kit from Promega was used ...