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Citations for Promega :
801 - 850 of 4999 citations for 6 Bromo 3 3H imidazol 4 ylmethylene 1 3 dihydro indol 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... or the QuantiFluor ONE dsDNA System (Promega). Indexed amplicons were pooled at equimolar concentration ...
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bioRxiv - Neuroscience 2021Quote: ... The CytoTox-ONE Membrane Integrity Assay (Promega) was performed according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... One microgram of sequencing grade trypsin (Promega) dissolved in water was added to the protein solution and the specific proteolytic digestion proceeded for 16 h at 37 °C in a thermomixer (Thermo Fisher Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 30 μL ONE-Glo reagent (Promega, E6120) was added to each well ...
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bioRxiv - Molecular Biology 2020Quote: ... and 40 units of RNase ONE (Promega) for 1.5 hr at 4°C with end-over-end rotation ...
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bioRxiv - Neuroscience 2019Quote: ... together with one of the pGL4 (Promega) luciferase vectors generated with enhancer sequences as described above ...
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bioRxiv - Microbiology 2020Quote: ... One μg of sequencing grade trypsin (Promega) was then added per sample and digestion was carried out overnight at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... and 100 µl One-Glo-EX (Promega) was added to the cells in equivalent MEM with no serum volume and incubated in the dark for 5 min ...
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bioRxiv - Physiology 2022Quote: CellTiter 96 AQueous One Solution Reagent (Promega) was used to assess the effect of EVs on cell proliferation rate in in vitro autotransplantation approach (n=4) ...
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bioRxiv - Genetics 2023Quote: CellTiter 96 AQueous One Solution (Promega G3580) was added to fresh BM-MSC complete growth media ...
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bioRxiv - Cancer Biology 2023Quote: ... CellTitre 96 AQueous One Solution MTS (Promega) was added to cells plated at low density according to manufacturer’s instructions and incubated in darkness in a humidified incubator for 3h at 37°C ...
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bioRxiv - Microbiology 2023Quote: CellTiter 96 AQueous One Solution (G3580, Promega) was used ...
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bioRxiv - Cancer Biology 2024Quote: ... CellTiter 96 Aqueous One Solution Assay (Promega) was added (20 μL per well) ...
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bioRxiv - Microbiology 2023Quote: ... One μg of sequencing grade trypsin (Promega) was then added per sample and digestion was carried out overnight at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Plasmid DNA (6 μg) was delivered into U2OS cells using FuGENE 6 (Promega) and 2.5 μg of plasmid DNA was delivered into HeLa cells using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... The primary MEFs were immortalised by transfection of 2 μg of simian virus 40 large T-antigen-expressing vector employing Fugene 6 Transfection Reagent (Promega, E2311) followed by five rounds of a 1 in10 split to achieve 1/100,000-fold splitting ...
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bioRxiv - Biophysics 2023Quote: ... transfection was carried out by adding 2 μg of freshly prepared bacmid DNA and 6 μL of FuGene HD transfection reagent (Promega, E2311), following the instructions provided by the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... and an empty vector up to 1 μg of total DNA using FuGENE 6 (Promega). Sixteen hours later ...
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bioRxiv - Genomics 2022Quote: ... Quantification of DNA was measured using fluorescence intensity of Quantifluor One dsDNA dye (Promega Quantifluor One dsDNA dye; E4871), inside a 0.5 milliliter thin-walled PCR tube ...
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bioRxiv - Molecular Biology 2021Quote: The pGL-AP-1 plasmid containing 6 consensus AP-1 binding sites was co-transfected with the pRL-CMV plasmid (Promega) into cells using TransIT 2020 transfection reagent (Mirus Bio ...
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bioRxiv - Microbiology 2022Quote: ... The samples were diluted with 225 μL of 57.6 mM NH4HCO3 containing 1 mM TCEP and the mixtures treated with 1 μg of sequencing grade modified trypsin (Promega), incubated at 37 °C overnight and then acidified by addition of formic acid to 0.1 % v/v and stored at −20 °C ...
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bioRxiv - Immunology 2020Quote: ... Cells were then incubated with CellTiter 96 Aqueous One Solution Proliferation Solution for 1 hour (Promega, Fitchburg, WI). Absorbance of the solution was then read at 490 nm ...
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bioRxiv - Plant Biology 2020Quote: ... corniculatus plants was synthesized from 3 μg of total RNA using a Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT) (Promega, WI, USA) and 100 pmol of random hexamers (Pharmacia Biotech) ...
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bioRxiv - Genomics 2021Quote: ... Cells were then grown for 3 days at which point the viability was measured using a CellTiter-Glo assay (Promega Cat# G9241) read out on a standard luminometer ...
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bioRxiv - Biochemistry 2020Quote: ... or PACAP (10 nM final) and assayed 3 hours later with the Nano-Glo® Dual-Luciferase® Reporter System (Cat# N1630; Promega) on a Berthold Sirius luminometer to quantify ERK activation.
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bioRxiv - Biophysics 2020Quote: ... P1KO cells were transiently transfected in a 6-well plate (the cells were seeded 8 h before transfection) in the presence of ruthenium red (10 μM) and mouse Piezo1 (3 μg) using Fugene6 (Promega, Madison, WI). 20–30% of cells showed positive GFP expression indicating successful Piezo1 transfection.
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bioRxiv - Biochemistry 2022Quote: The 4330bp PCR product amplified from M13mp18 by using primers: oM13-5-27 and oM13-3-24 was inserted into pGEM®-T Easy(PROMEGA #A137A). The resultant plasmid is named as pM13 ...
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bioRxiv - Microbiology 2022Quote: ... PAO1-WT and few colonies that grew in 3 mM 22D-chelated condition were isolated and gDNA extracted using Wizard® Genomic DNA Purification Kit (Promega, USA) for use in whole genome sequencing ...
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bioRxiv - Neuroscience 2022Quote: ... and mouse cholecystokinin (CCK, 3-688 of NM_031161) were PCR-amplified and subcloned into the pGEM-T Easy vector (Promega, Fitchburg, WI, USA). Digoxigenin (DIG)-labeled anti-sense probes were prepared using DIG RNA Labeling Mix (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... The NanoLuc and Fluc luciferase activities were measured 3 days after transfection using the Nano-Glo® Dual-Luciferase® Reporter Assay System (Promega). The firefly (Fluc ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... RBD-R: 5′-TGC CTC GAG CTC AAG TGT CTG TGGATC AC-3′) into pGEM T-easy vector (Promega, Madison, WI, USA). A standard curve was generated by determining the copy numbers derived from serial dilutions of the plasmid (103–109 copies) ...
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bioRxiv - Microbiology 2020Quote: ... 3’-untranslated region (3’-UTR) fragment of IκBα (human/rat) was inserted into the pGL3-basic vector (firefly luciferase; Promega, Madison, WI, USA), which was obtained from General Biosystems (Anhui ...
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bioRxiv - Cancer Biology 2022Quote: ... we followed the genotyping method for KrasG12D mutation previously described.3 Genomic DNA was isolated from formalin fixed paraffin embedded thymus slides using ReliaPrep FFPE gDNA MiniPrep System (Promega, Madison, WI). The DNA was then amplified using polymerase chain reaction (PCR ...
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bioRxiv - Cancer Biology 2022Quote: Apoptosis induction in response to SRRM1 silencing in leukemia cell lines (25,000 cells/well onto white-walled multiwell luminometer plates) was performed by using Caspase-Glo® 3/7 Assay (Promega Corporation, #G8091) as previously reported (67) ...
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bioRxiv - Cancer Biology 2019Quote: ... These were transfected into Tet-On HEK-293TLDcells [10] by incubating 3 µg plasmid DNA with 9 µL Fugene HD (Promega, Madison, WI) in 100 µL Optimem for 15 min ...
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bioRxiv - Biochemistry 2021Quote: The promoter fragment of luciferase reporter plasmid pGADD34-0.5k generated by Eurofins Japan (Tokyo, Japan) was subcloned into a pGL-3 vector (Promega, Madison, WI, USA) by restriction enzyme cutting site KpnI/HindIII ...
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bioRxiv - Molecular Biology 2021Quote: ... A total of 24 clones (4 holobionts x 3 replicates x 24 clones = three 96-well plates) from each amplicon were amplified directly with GoTaq polymerase (Promega, Lyon France) in a 25 μL reaction volume using the following PCR parameters ...
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bioRxiv - Molecular Biology 2022Quote: ... SEA (0.2 mg/ml) and soluble schistosomula antigens (0.2 mg/ml) was determined using the Caspase-Glo® 3/7 Assay System (Promega, Sydney, Australia) according to the manufacturers’ instructions.
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bioRxiv - Evolutionary Biology 2023Quote: The 419 bp sequence of 3’UTR containing predicted target sites for miR-252 in CcTRPM was cloned into the pmirGLO vector (Promega, Wisconsin, USA) downstream of the luciferase gene to construct the recombinant plasmid of CcTRPM-3’UTR-pmirGLO using the pEASY®-Basic Seamless Cloning and Assembly Kit ...
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bioRxiv - Genomics 2024Quote: ... and 1,750 ng landing pad G384A vector template using 6 μL Fugene 6 (Promega) following the manufacturer’s protocol ...
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bioRxiv - Physiology 2019Quote: ... 2 μL sequencing-grade trypsin (1 μg/μL; Promega) was added for the digestion at 37°C for 12 h ...
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bioRxiv - Genomics 2023Quote: ... 0.25 μl of 1% Digitonin in DMSO (Promega (2%), Cat ...
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bioRxiv - Molecular Biology 2020Quote: ... (49) as a negative control (2 μg of DNA per well of 6 well plate) with FuGene transfection reagent (Promega, Madison, WI) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated for an additional 2 – 6 days and cell growth was quantified using CellTiter-Glo® 2.0 Reagent (Promega, Cat. #924C). For immunoblot analysis ...
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bioRxiv - Physiology 2023Quote: ... UMR106 cells were plated at 4 × 105 per well in a 6-well plate and transfected with 2 µg of NFAT5 gRNAs per well using the FuGENE® HD Transfection Reagent (Promega, USA). 24 h post-transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... These cells were transfected in 70% confluent 6-wells TC-treated plates with 2 µg TGFβ receptor-Nanoluciferase constructs (constructs were provided by Promega and Promega R&D) using a 1 µg DNA ...
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bioRxiv - Cell Biology 2021Quote: ... or Fugene 6 (Promega #E2691) for 5 hours ...
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bioRxiv - Cell Biology 2020Quote: ... FuGENE 6 Transfection Reagent (Promega) was used according to the manufacturer’s protocol and the phenotype inspected 24 or 48 h after transfection ...
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bioRxiv - Genomics 2020Quote: ... using Fugene 6 (Promega, E2691). Virus was then harvested and applied to Scc1-EGFP-AID/dCas9-KRAB cells ...
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bioRxiv - Neuroscience 2020Quote: ... using FuGENE®-6 (Promega). Supernatants containing viral particles were aseptically collected 3 days post-transfection ...