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Citations for Promega :
851 - 900 of 2047 citations for Pops Par Solution Unlabeled 2000 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... containing 10% (vol/vol) FBS and 2% (vol/vol) GloSensor cAMP stock solution (Promega). The cells were then incubated for 2 hours at room temperature in the dark ...
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bioRxiv - Cell Biology 2020Quote: ... MTS assay (G3582, CellTiter 96® Aqueous One Solution Cell Proliferation Assay, Promega, UK) was performed for colorimetric quantification of viability ...
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bioRxiv - Systems Biology 2019Quote: ... cell viability was determined using CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega) kit and absorbance was quantified on a microplate reader (TECAN) ...
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bioRxiv - Developmental Biology 2019Quote: ... after which the DNase reaction was stopped by the addition of Stop Solution (Promega). Synthesized riboprobes were purified with the use of a ProbeQuant G-50 column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... plates were removed from the incubator and 100 μL of NanoGlo IC50 solution (Promega NanoGlo reaction mixture diluted 10-fold in PBS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... CellTiter 96® Aqueous One Solution Cell Proliferation Assay Kit was purchased from Promega AG (Dübendorf ...
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bioRxiv - Physiology 2020Quote: The protein solution samples were hydrolyzed into peptide segments by trypsin (V9012, Promega (Beijing) Biotech Co. ...
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TRANSITION OF PODOSOMES INTO ZIPPER-LIKE STRUCTURES IN MACROPHAGE-DERIVED MULTINUCLEATED GIANT CELLSbioRxiv - Cell Biology 2020Quote: ... PCR was performed with the generated cDNA and premixed 2× Taq polymerase solution (Promega) in an MJ Mini Thermal Cycler (BioRad) ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 50 μL trypsin solution (0.01 μg/μL sequencing grade modified trypsin (Promega, #V5111) in 50 mM NH4HCO3 ...
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bioRxiv - Microbiology 2021Quote: Cell viability was assessed using the CellTiter 96 AQueous One Solution Reagent (Promega, Madison). This method determines the number of viable cells based on conversion of formazan product from 3-(4,5-dimethylthazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolim by nicotinamide adenine dinucleotide phosphate (NADPH ...
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bioRxiv - Genetics 2019Quote: ... cell viability was measured using CellTiter 96® AQueousOne Solution Cell Proliferation Assay (Promega) according to manufacturer’s specifications ...
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bioRxiv - Immunology 2022Quote: ... CellTiter96® AQueous One Solution Cell Proliferation Assay (MTS) (Promega Corp, Madison, Wisconsin, USA) was added to each well as recommended by manufacturer and incubated for 1 h at 37°C ...
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bioRxiv - Plant Biology 2022Quote: ... The digestion solution was diluted to <1 M urea upon addition of trypsin (Promega, V511A ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.1% Tween) and a final incubation at room temperature in NBT/BCIP solution (Promega) till color developed ...
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bioRxiv - Neuroscience 2023Quote: ... eluted in-solution samples were alkylated with N-Ethylmaleimide and digested with trypsin (Promega) using micro S-trap column (ProtiFi) ...
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bioRxiv - Biophysics 2023Quote: ... the desired volume of protein solution was incubated with Factor Xa (Promega, Madison, WI). All experiments were carried out in a tris buffer (150 mM NaCl ...
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bioRxiv - Developmental Biology 2023Quote: We used the CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, #G3582) for determining the number of viable cells ...
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bioRxiv - Cancer Biology 2023Quote: Cell viability was determined using CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega), utilising MTS reagent ...
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bioRxiv - Cell Biology 2023Quote: ... Subsequent digestion was performed with Trypsin/LysC solution [100ng/μl 1:1 Trypsin (Promega):LysC (FujiFilm ...
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bioRxiv - Genomics 2024Quote: ... Proliferation was measured using the CellTiter96 AQueous One Solution Cell Proliferation Assay (Promega, G3582) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: Cell viability was evaluated using the CellTiter 96® AQueous one solution reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Membranes were washed in TBST and probed with anti-mouse HRP secondary antibody (Promega; 1:2000 in 5% BSA in TBST) for 1 h at room temperature followed by development in ECL (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... and 1:2000 RealTime-Glo MT Cell Viability Assay Substrate (to visualise Nluc, first diluted 1:1 in DMSO, Promega G9711).
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bioRxiv - Developmental Biology 2021Quote: ... cells were transfected with 750 ng of the pMXs-IP vectors with 2.3 μl FuGENE 6 (Promega, E2691). The culture medium was replaced with fresh DMEM with 10% FBS and C2C12 cells were seeded at a concentration of 1×105 cells/well in a 12-well plate on day 3 ...
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bioRxiv - Molecular Biology 2020Quote: ... beads were resuspended in 50 μL 50 mM TEAB pH 8.5 containing 0.1% Na-deoxycholate and 200 ng of trypsin (Promega), before incubation overnight at 37 °C with periodic shaking (Eppendorf Thermomixer) ...
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bioRxiv - Cancer Biology 2019Quote: ... Transfection was performed using FuGENE HD transfection reagent (500 ng DNA: 2.5 µl FuGENE reagent) (Promega, Mannheim, Germany).
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bioRxiv - Biochemistry 2020Quote: ... Cells were then transfected with 1500 ng pcDNA-FLAG-HA-sscGAS plasmid complexed with FuGene 6 reagent (Promega) according to manufacturer’s instructions or treated with FuGene 6 alone as a negative control ...
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bioRxiv - Microbiology 2020Quote: RNA (400 ng) was reverse transcribed into cDNA using random oligos and the GoScript Reverse Transcriptase kit (Promega). The cDNA was probed using primers specific to target genes in the StepOnePlus Real Time PCR system as described above ...
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bioRxiv - Microbiology 2021Quote: ... and 800 ng pSPAX2 were co-transfected into HEK 293T cells using the FuGENE HD Transfection Reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were digested with shaking overnight at room temperature in 300 ng of sequencing grade modified trypsin (Promega). Samples were then acidified with trifluoroacetic acid (TFA ...
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bioRxiv - Molecular Biology 2022Quote: ... the proteins were digested overnight at 37 °C with 6 ng μL-1 of Asp-N (Promega, UK). Peptides were extracted in 2 % v/v formic acid ...
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bioRxiv - Systems Biology 2022Quote: ... followed by 4-fold dilution and an overnight digestion with 500 ng trypsin (Promega, Charbonnieres les Bains, France). Peptide mixtures were then desalted on C18 spin-column and dried on Speed-Vacuum.
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was synthesized from 100-500 ng of total RNA using the GoScript reverse transcription reaction kits (Promega). Singleplex quantitative real-time polymerase chain reaction (qPCR ...
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bioRxiv - Molecular Biology 2019Quote: ... was added to 500 ng of the cDNA-fragments in T7 transcription reaction buffer (Promega, Cat no. P2075), and incubated for 2 hours at 37°C in the absence or presence of N6-methyladenosine (Jena Biosciences ...
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bioRxiv - Molecular Biology 2019Quote: 1875 ng of circular plasmids were incubated at 37°C with 50 U of T3 RNA Polymerase (Promega) in a transcription buffer (40 mM Tris-HCl pH 7.9 ...
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bioRxiv - Cancer Biology 2019Quote: ... Dilute proteins were digested overnight with 5 μl of 25 ng/μl trypsin gold (Promega, Madison, WI, USA) and desalted with a ZipTipC18 pipette tip (Millipore ...
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bioRxiv - Microbiology 2020Quote: The protein samples were digested with 500 ng of sequencing grade trypsin (V511, Promega Co., Madison, WI, USA) and incubated at 37 °C following the protocol described by Shevchenko et al.22 ...
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bioRxiv - Microbiology 2019Quote: ... We reverse-transfected cells in each well with 10 ng of pGL4.23-CMV/NP-NLuc or pNL1.1/NLuc (Promega) negative control and 100 ng of pGL4.23-CMV/NP-HaloTag or pcDNA3.3/eGFP negative control ...
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bioRxiv - Biochemistry 2019Quote: ... 0.2 μg pLEXm plasmid encoding WT or mutant KAP1 and 0.4 ng plasmid encoding Renilla luciferase using 1.5 μl of FuGENE 6 (Promega). Luciferase activity was measured 48 h post-transfection in cell lysates with the Dual Luciferase assay kit (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... The volume of each binding reaction was 100µl in SEQRS buffer containing 200 ng yeast tRNA competitor and 0.1 units of RNase inhibitor (Promega). The samples were incubated for 30min at 22°C prior to magnetic capture of the protein-RNA complex ...
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bioRxiv - Bioengineering 2020Quote: ... PCR reactions were carried out with 100 ng genomic DNA or cDNA in the GoTaq Master Mix (Promega) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2021Quote: ... 800 ng of total RNA were freed from genomic DNA using the RQ1 RNase-Free DNase Kit (Promega). Reverse transcription was performed using 145,4ng of the total DNase-treated RNA using the TaKaRa PrimeScript RT Master Mix Kit ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was generated from 20-100 ng of RNA using the GoTaq 2-step RT-qPCR System (Promega). qPCR was performed with SYBR Green on a StepOnePlus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was generated from 20-100 ng of RNA using the GoTaq 2-step RT-qPCR System (Promega). qPCR was performed with SYBR Green on a StepOnePlus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Cell Biology 2020Quote: ... The dried gel pieces were re-swollen by incubation in 100 mM ammonium bicarbonate pH 8.0 supplemented with trypsin (12.5 ng/μL; Promega) for 1 h at 4 C and then incubated overnight at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were co-transfected with 50 ng of the Renilla luciferase transfection control plasmid pRL-CMV (Promega #E2261) and 1 μg of either the ERα reporter plasmid XETL (here referred to as ERE-Luc ...
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bioRxiv - Molecular Biology 2023Quote: ... Enzymatic digestion was performed at 37°C and overnight with 500 ng trypsin (Promega, Charbonnieres les Bains, France). Peptide mixtures were then desalted on C18 spin-column and dried in a Speed-Vacuum.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The following amounts of DNA were used per well: 60 ng of pGloSensor-22F cAMP plasmid (Promega, E2301), 100 ng of receptor or empty vector (control ...
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MET functions in tumour progression and therapy resistance are repressed by intronic polyadenylationbioRxiv - Molecular Biology 2023Quote: ... PCR reactions were performed using 10 ng of cDNAs (RNA equivalent) and the GoTaq DNA polymerase (Promega, # M8295). For each gene ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were transfected with the following plasmids and amounts per well: 25 ng SRE-Luc reporter (E134A, Promega), 75 ng Gαi or Gαi QL in pcDNA3.1+ ...