Labshake search
Citations for Promega :
851 - 900 of 1357 citations for 6 TERT BUTYLQUINOLINE 98.0+% GC T since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: Approximately 105 ng of full-length PCR product was ligated into the pGEM-T Vector System (Promega, Madison, WI), at a 3:1 molar ratio of insert:vector and incubated overnight at 4 °C ...
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bioRxiv - Plant Biology 2021Quote: ... Undigested bands from the restriction enzyme digestion were purified and cloned into the pGEM-T easy vector (Promega, USA) or using Zero Blunt™ TOPO™ PCR Cloning Kit (Invitrogen™ ...
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bioRxiv - Plant Biology 2020Quote: ... Absolute quantification was obtained with DNA fragment from the Map gene cloned in pGEM®-T Easy vector (Promega) with 10 to 108 copies of the plasmid added on each PCR plate ...
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bioRxiv - Cell Biology 2021Quote: ... The tacc3 construct used for a hybridization probe was subcloned into the pGEM T-easy vector (Promega, Madison, WI). The Xenopus twist hybridization probe was a gift from Dr ...
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bioRxiv - Microbiology 2020Quote: ... and the PCR products of 5’ RACE were cloned using pGEM®-T Easy Vector Systems (Promega, WI, USA). Purified PCR fragments and pGEM cloning 5’-terminal sequence of BToV were sequenced using primers designed for every ∼300 bp of the BToV genome ...
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bioRxiv - Plant Biology 2021Quote: ... the full length of the native GmDGAT1A coding region was cloned into a pGEMR-T Easy vector (Promega, USA). Site-directed mutagenesis was performed by inverse PCR using PrimeSTAR GXL polymerase (Takara ...
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bioRxiv - Cancer Biology 2019Quote: ... washed thrice with TBS-T and incubated for 1 hour with HRP-conjugated secondary antibody (Promega, Madison, WI, USA). After four additional washes ...
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bioRxiv - Plant Biology 2020Quote: ... The PCR product was subjected to adenine overhanging and inserted into pGEM T-easy vector (Promega, Madison, WI, USA) for sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... selected fragments of alternatively spliced exons (Supplementary Table 7) were cloned into the pGEM®-T Easy Vector (Promega). To generate the DNA templates for transcription ...
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bioRxiv - Molecular Biology 2019Quote: ... The two fragments were then ligated using an overlap extension PCR reaction and ligated into pGEM/T-Easy (Promega). Positive clones were digested with AscI and SalI and the dropout was subsequently ligated into an AscI- and SalI-linearized pENTR-PpU6-sgRNA-NGG plasmid.
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bioRxiv - Plant Biology 2021Quote: ... LaMATE2ORF sequence was cloned in pGEM-T easy vector (for primers see Table S1, Promega Italia Srl, Milan, Italy) and deposited in the NCBI database (KY464927).
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bioRxiv - Developmental Biology 2021Quote: ... mytiligera cDNAs using gene-specific primers and cloned into pGEM-t vector using the manufacturer’s protocol (Promega; CAT #A1360). Vectors were transformed into E ...
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bioRxiv - Microbiology 2021Quote: ... and NS1 (3′) sequences were determined from purified product cloned into the pGEM-T vector by TA-cloning (Promega) according to the manufacturer’s guidelines.
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bioRxiv - Microbiology 2020Quote: ... This amplified PCR product was then cloned into the pGEM-T Easy Vector using T4 ligase (Promega, Madison, WI), resulting in the construction of pCjNC110::cat which was then transformed into chemically competent E ...
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bioRxiv - Neuroscience 2022Quote: ... the double stranded DNA HDR donor was prepared in a step-wise manner in pGEM-T Easy vector (Promega) by first cloning in approximately 800bp long homology arms that were amplified from C57BL6/N-derived genomic DNA (Left arm ...
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bioRxiv - Genetics 2022Quote: ... The obtained DNA fragment containing the gtr1Q86L allele was cloned into the pGEM-T vector (Promega, Madison, WI, USA), verified by Sanger sequencing and used to co- transform protoplasts of the F ...
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bioRxiv - Plant Biology 2022Quote: ... Promoter fragment of proCRF1-1.2 kb and proPIN1-1.3 kb was amplified and ligated into TA vector (pGEM®-T Easy Vector, Promega). The proCRF1::GUS and proPIN1::GFP was constructed by replacing CaMV 35S promoter of pCAMBIA1304 with proCRF1-1.2kb and proPIN1-1.3kb ...
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bioRxiv - Neuroscience 2022Quote: Riboprobes for in situ hybridisation were generated by cloning transcript-specific PCR products into pGEM-T-Easy (Promega, A1360) using the TA-cloning method ...
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bioRxiv - Microbiology 2022Quote: PCR amplicons were gel extracted using the QIAquick gel extraction kit and ligated into pGEM-T Easy plasmid (Promega). Vectors containing the PCR products were then cloned into chemically competent E ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR fragments with expected size were purified and TA cloned in the pGEM-T Easy Vector (Promega, Madison, WI) for DNA sequencing ...
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bioRxiv - Molecular Biology 2022Quote: ... individual target sequences were PCR amplified and the amplicons were sub-cloned into separate pGEM-T Easy vector (Promega) at TA cloning site ...
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bioRxiv - Microbiology 2023Quote: ... jejuni NCTC11168 WT (CSS-5295) gDNA using oligonucleotides CSO-4744 x 4745 and ligated into pGEM-T Easy (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed 3 times for 10 min in TBS-T and subsequently incubated with species-specific HRP-conjugated secondary antibodies (1:10,000; Promega) in 5% non-fat dry milk -TBS-T for 1 h at room temp ...
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bioRxiv - Microbiology 2023Quote: Approximately 100 ng of full-length PCR product was ligated into the pGEM-T Vector System (Promega, Madison, WI), at a 3:1 molar ratio of insert ...
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bioRxiv - Neuroscience 2023Quote: ... Inserts (primers and size are presented in Supplementary Table S5) were cloned into pGEM-T Easy (Promega, Madison, WI) vector and confirmed by restriction and sequencing analysis ...
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bioRxiv - Genomics 2023Quote: ... The total cellular DNAs of the activated CD4+ T cells were isolated using a DNA extraction kit (Promega Wizard) for analysis.
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bioRxiv - Microbiology 2023Quote: ... in TBS-T followed by a washing step for 30 min with TBS-T and a final 60 min incubation with with 1:10.000 horseradish peroxide-conjugated anti-rabbit IgG (Promega) in TBS-T ...
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bioRxiv - Cell Biology 2020Quote: ... Transfection was performed in the 6-well culture plate using FuGene-HD (Promega, E2311) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Retrovirus was produced in Phoenix Amphotropic cells by transfection with FUGENE 6 (Promega E2692) of pLPC-TRF2 or pLPC-Empty ...
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bioRxiv - Cell Biology 2021Quote: ... and a pcDNA5/FRT plasmid of interest using the Fugene 6 transfection kit (Promega) or Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... Plates were collected at day 6 and measured by CellTiter-Glo (Promega #PR-G7573) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: HEK 293T cells grown on 10 cm dish were transfected using FuGENE 6 (Promega) with the transfer vector pxPAX2 (Addgene #12260 ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were transiently transfected with 0.4 µg DNA using FuGENE 6 transfection reagent (Promega) in a 1:3 µg DNA:µL FuGENE ratio 48 h before electrophysiology measurements were performed.
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bioRxiv - Immunology 2020Quote: ... China) or the D614G variant (constructed by site-directed mutagenesis) using FuGENE 6 (Promega). The next day ...
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bioRxiv - Developmental Biology 2020Quote: ... For the immunolabeling experiment HeLa cells were transfected with plasmids using FuGENE 6 (Promega). For streptavidin pull-down assays from HEK293T cells ...
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bioRxiv - Genetics 2021Quote: ... Plasmid transfection of U2OS cells was carried out using FuGENE 6 Transfection Reagent (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Transfection with plasmid was performed following standard protocols for Fu gene 6 (Promega, #E2691). Cells were selected for stable expression using Geneticin (G418 sulfate ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μg of both plasmids was mixed with 6 μl FuGENE reagent (Promega E269A) in 200 μl serum free DMEM and incubated for 30 minutes before adding the transfection mix to the cells ...
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bioRxiv - Physiology 2021Quote: ... allowing measurement of 2-deoxyglucose-6-phosphate using the Glucose Uptake-Glo assay (Promega) and a FLUOstar Omega luminescence plate reader.
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bioRxiv - Biophysics 2021Quote: ... U2OS cells transfection with engineered lentiCRISPRv2 were done using FuGENE 6 transfection reagent (Promega). Positive clones were selected using puromycin and subsequently isolated.
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bioRxiv - Cell Biology 2022Quote: ... Bacmids were transfected to Sf9 insect cells by lipofection (FuGENE 6 Transfection Reagent, Promega), to generate baculovirus ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmid transfection of U2OS cells was carried out using FuGENE 6 Transfection Reagent (Promega) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... 1 μg of both plasmids was mixed with 6 μl FuGENE reagent (Promega E269A) in 200 μl serum free DMEM and incubated for 30 min before adding the transfection mix to the cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Plates were collected at day 6 and measured by CellTiter-Glo (Promega #PR-G7573). Cell viability values were analyzed following blank cell deductions and normalization to vehicle readings ...
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bioRxiv - Microbiology 2023Quote: ... cells were transfected with MX2-mScarlet vector using FuGENE 6 transfection reagent (#E2691, Promega) following the manufacturers protocol ...
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bioRxiv - Biochemistry 2024Quote: ... 24 hours after transient transfection with mouse or human ENPP3 via Fugene 6 (Promega), the media was gently removed and replaced with serum-free DMEM supplemented with 1% insulin-transferrin-selenium-sodium pyruvate (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected with TPMT-FLAG or TPMT A80P-FLAG with FuGENE 6 (Promega). 24 h post transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 ng/μl of mRNA and 6 ng/μl QuantiLum Recombinant Luciferase protein (Promega) were coinjected ...
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bioRxiv - Plant Biology 2020Quote: ... PCRs were used to assay for wild-type or T-DNA insertion alleles in 20 μL reactions using GoTaq Green Master Mix (Promega) using manufacturer’s instructions and 30 cycles as follows ...
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bioRxiv - Microbiology 2021Quote: Promoter fusions to lacZ were constructed by amplification of promoter regions from genomic DNA with the specified primers (Table S4) and ligated into pGEM-T Easy (Promega) for amplification and collection ...