Labshake search
Citations for Promega :
8851 - 8900 of 9065 citations for Total Carbohydrate Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... The efficiency and specificity of the designed primers was tested through PCR using the GoTaq Green Master kit (Promega, Madison, USA) with thermal cycling conditions of 2 min at 95 °C ...
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bioRxiv - Genomics 2022Quote: ... DNA was extracted from a piece of caudal fin using a Maxwell® RSC Blood DNA Kit (Promega, Madison, WI, USA). Extractions were performed following the manufacturer’s instructions with the exception of a longer two-hour lysis step ...
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bioRxiv - Evolutionary Biology 2022Quote: ... H12) of each 96 well plate were pooled prior to DNA extraction using Wizard Genomic DNA purification kit (Promega, Madison, Wisconsin). The P0 region including the beginning of galK was amplified for 25 PCR cycles using primers deep_seq_Fw and deep_seq_Rv carrying 5’ adaptors for Illumina sequencing ...
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bioRxiv - Molecular Biology 2022Quote: ... the mycelium was harvested and then used in the RNA extraction following the protocol provided in the Maxwell® 16 LEV simplyRNA Cells Kit (Promega). RNA concentrations were measured by NanoDrop and purified RNA was stored at -80°C ...
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bioRxiv - Microbiology 2022Quote: ... The cells were then pelleted and used for genome extraction using the Wizard Genomic DNA Purification Kit (Promega, Madison, WI, USA) and Qiaquick PCR purification kit (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... phosphorothioated and phosphorylated/phosphorothioated forward primers as well as a phosphorylated reverse primer were done in qPCR reaction mixtures including 1X reaction mixture of GoTaq 2-Step RT-qPCR kit (Promega, USA), 400 nM forward and reverse primers and in final volume of 20 μL ...
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bioRxiv - Microbiology 2023Quote: ... One aliquot was used for DNA extraction using the Promega Wizard® Genomic DNA Purification kit (Promega Corporation, Madison, WI, USA) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... of virus pool stock was used for viral RNA extraction by GeneAid viral RNA extraction kit (GeneAid) and converted to cDNA using ImProm-II™ reverse transcription system (Promega) according to manufacturers’ protocols ...
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bioRxiv - Neuroscience 2023Quote: Four to five organoids were physically lysed using a pipette and purified for RNA on the Promega Maxwell RSC48 in conjunction with the Maxwell RSC Simply RNA Tissue kit (Promega, AS1340). cDNA synthesis was performed using the SuperScript III First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... and 3μg RNA was used to synthesize the first strand cDNA in a 20μl reaction using the Reverse Transcription System kit (Promega, Beijing, China). The GoTaq® qPCR and RT-qPCR Systems kit (Promega ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumor DNA was extracted from parts of FFPE containing a high fraction of tumor cells using Maxwell® RSC DNA FFPE Kit (Catalog number: AS1450, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... supernatant was collected from the replicon-transfected cells and assayed for Renilla luciferase activity according to kit instructions (Promega, cat. E2810).
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bioRxiv - Biochemistry 2024Quote: ... eloB and eloC) were purified on an agarose gel using the Wizard ® SV gel and PCR clean-up system purification kit (Promega). After restriction with the corresponding restriction enzymes (New England Biolabs ...
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bioRxiv - Zoology 2024Quote: ... The amplicon target was amplified from WNV cDNA using the qPCR primers with the forward primer flanked by T7 sequence (5’-TAATACGACTCACTATAGGGATTCGGGAGGAGACGTGGTA-3’) and transcribed using T7 RiboMAX Express Large Scale RNA Production System kit (Promega, France). RNA was purified by ethanol precipitation ...
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bioRxiv - Microbiology 2023Quote: ... We used 1 ml of cultures and isolated the genomic DNA using the Wizard Genomic DNA Purification Kit (Promega; Madison, WI). We used the DNA with the Illumina DNA Prep kit (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: Genomic DNA was isolated from the 102 human FFPE colorectal tissue samples using the Maxwell® RSC Blood DNA Kit (Promega) on a Maxwell® 16 MDx (Promega) ...
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bioRxiv - Cell Biology 2023Quote: ... Amplified ORFs were then used as templates in a PCR reaction to amplify a full-length mutant amplicon followed by their purification using Wizard SV Gel and PCR Clean-Up kit (Promega, USA). These ORFs were digested with KpnI and BamHI enzymes and ligated into similarly digested pDsRed vector ...
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bioRxiv - Genomics 2023Quote: ... We added 200 µL of PBS to each cell pool and performed the 22 DNA extractions using the Buccal Swab protocol of the ReliaPrep™ gDNA Tissue Miniprep System kit (Promega). We adjusted the lysis time to two hours before performing the RNAse treatment ...
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bioRxiv - Microbiology 2024Quote: ... Treatments did not affect bacterial or cell viability as assessed by Lactate dehydrogenase release in the culture supernatant (LDH cytotoxicity kit®, Promega, according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... control Fiber-seq on dechromatinized DNA was performed on genomic DNA purified from Drosophila S2 cells using the Promega Wizard HMW DNA Extraction Kit (Promega A2920). 2 µg of DNA was used in a final volume of 60 µL in Buffer A with 0.8 mM S-adenosylmethionine and 200 U Hia5 ...
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bioRxiv - Immunology 2024Quote: ... T cell cytotoxicity was assessed by quantification of LDH levels in the supernatants using the LDH-Glo cytotoxicity kit (Promega, J2380) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2023Quote: ... 1 µl of duplex DNA was ligated into 25 ng of pBract214m-HvCas9-HSPT vector linearized with AarI using the ligation reagents and protocol described in pGEM®-T Easy Vector System kit (Promega). The ligation reaction was incubated overnight at 4°C then transformed into competent E ...
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bioRxiv - Microbiology 2023Quote: ... 3 ml overnight cultures were subjected to DNA extraction using an automated Maxwell® 16 Tissue DNA purification kit and following the manufacturer’s instructions (Promega, UK). For long-read complete genome analysis ...
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bioRxiv - Biochemistry 2023Quote: ... a calibration curve for SAH was constructed in two independent experiments using from 0 to 5 μM SAH according to the manufacturer’s protocol of the MTase-Glo kit (Promega, Wisconsin, USA). The signal was detected using a Tecan Spark microplate reader (Tecan Japan Co. ...
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bioRxiv - Cancer Biology 2023Quote: ... Organoids were cultured for another 5 days before proceeding with microscopy image and viability test by CellTiter-Glo 3D kit (Promega: G9681) following manufacturer’s instructions.
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bioRxiv - Biophysics 2023Quote: ... Subsequently we ran the digested fragment on a 1% TAE agarose gel and the desired ∼14 kbp DNA fragment was isolated from an agarose gel using a gel purification kit (Promega, A9282).
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bioRxiv - Genomics 2023Quote: ... DNA desulphonation and cleanup steps were immediately performed following the manufacturer instructions of the MethylEdge® Bisulfite Conversion System kit from Promega. After the column cleanup ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultured in a 6-well plate and transfected with 10 µg of linearised plasmid expressing the SV40 Large T antigen using the FuGene Transfection Kit (Promega, #E2691) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... prior to firefly and Renilla luciferase activity measurements which were performed in triplicate using the Dual-GLO kit (Promega cat. #E2920). The whole procedure was repeated three times independently for each construct.
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bioRxiv - Developmental Biology 2023Quote: Notch reporter 4xcsl::H2B-mvenus plasmid(Nowotschin et al., 2013) was injected at 30ng/µl after purification using the PureYield Midiprep kit (Promega, #A2492). For antisense in situ hybridization probes ...
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bioRxiv - Cancer Biology 2023Quote: ... they were treated for the indicated times and viability was assessed using the CellTiter-Glo® 3D Cell viability kit (Promega), following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... MDA-MB231 cells were imaged by SEM and quantified by fluorescence analysis of DNA content with the QuantiFluor dsDNA kit (Promega, US). For detection of stem-like properties of cells in scaffolds ...
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bioRxiv - Bioengineering 2023Quote: ... Substrate-dependent differences of cell adhesion and growth were determined by fluorescence analysis of DNA content with the QuantiFluor dsDNA kit (Promega, US) after 5 h and 7 d of culture ...
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bioRxiv - Microbiology 2023Quote: DNA extractions for genomic sequencing were performed using Maxwell™ RSC Cell DNA Purification Kit and automated extraction on the Maxwell RSC 48 Instrument (Promega). The DNA concentration was evaluated using a Qubit dsDNA BR assay kit and Qubit Fluorometer (ThermoFisher) ...
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bioRxiv - Developmental Biology 2023Quote: ... Targeted sequence of each single clone was validated by Sanger sequencing after gDNA isolation through Wizard® Genomic DNA Purification Kit (#A1120, Promega). For Prkaa1 knockout ...
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bioRxiv - Cancer Biology 2023Quote: ... Two replicate electroporations were each divided into three wells for 48h of culture and readout using the Dual-Glo Luciferase kit (Promega; E2940) in a Bio-Tek Synergy HT Microplate Reader.
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bioRxiv - Neuroscience 2023Quote: ... equal amount of RNA (2 μg) from each sample was subjected to cDNA synthesis using reverse transcriptase Kit (Promega Corporation, USA) following standard procedures ...
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bioRxiv - Biophysics 2023Quote: ... from the access handles by running this on a 1% agarose gel and gel purify the right DNA fragment with a gel purification kit (Promega, A9282).
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bioRxiv - Genomics 2023Quote: ... Resulting genomic DNA was assessed for concentration and purity using Promega DNA quantification kit on the Promega Quantus Fluorometer (Promega, Germany) and measurement of A260/A280 ratio on the MultiSkan Sky High spectrophotometer (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: Genomic DNA was prepared from single cell suspensions of thymic leukemias using the Wizard Genomic DNA Purification Kit (Promega, Madison WI). PCR amplification of the Jak3 pseudokinase domain was performed using MyTaq HS Red Master Mix (FroggaBio ...
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bioRxiv - Microbiology 2023Quote: ... The amplified PCR fragments were checked for uniformity and size using 0.8% agarose gel electrophoresis and purified using a PCR fragment recovery kit (Promega, WI, USA). The recovered DNA fragments were sequenced using the MiSeq 500PE v.2 kit (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... ATRX 5ʹ-TGAAACTTCATTTTCAACCAAATGCTC-3ʹ and 5ʹ-ATCAAGGGGATGGCAGCAG-3ʹ All PCR reactions were performed using GoTaq® G2 DNA polymerase kit from Promega following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... Luciferase activity was assayed in an Omega luminescence plate-reader (Fluostar) with four different gains following the instructions of the Nano Dual-Luciferase® Reporter kit (Promega). A further correction for luminescence bleed-through and background signal was applied using the software developed by Mauriet al.58 Then NLuc signal was divided by the FLuc signal to normalize for the transformation efficiency.
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bioRxiv - Cancer Biology 2024Quote: ... The BSP amplification product was purified by Vazyme Biotech (China) amplification product purification kit and cloned into pGEM-Tasy-vector system (Promega, USA). and the percentages of methylated cytosines over total cytosines were calculated.
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bioRxiv - Microbiology 2024Quote: ... and tryptone (Becton Dickinson DifcoTM,)) overnight at 35°C and genomic DNA was extracted using the Wizard® Genomic DNA purification kit (Promega) as previously described [35] ...
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bioRxiv - Microbiology 2024Quote: DNA extraction of the bacterial strains isolated from oysters and cultivated on agar plates was carried with the Wizard® Genomic DNA Purification Kit (Promega) according to the manufacturer instructions ...
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bioRxiv - Physiology 2024Quote: Total RNA was isolated from snap frozen LV wall or kidney after mechanical grinding (Precellys Evolution, Bertin Technologies, France) using the Maxwell RSC microRNA Tissue kit (Promega, USA). RNA was quantified via NanoDrop (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Total RNA was extracted from 10 ⨯ 10 µm FFPE tissue sections using the Maxwell RSC FFPE RNA kit in accordance with the manufacturer’s manual (Promega, United States). RNA concentration was determined using a Qubit 3.0 Fluorometer using the Qubit RNA BR Assay Kit (Thermo Fischer Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: ... Larvae from progenies with low homing rates of dsxFGFP-null were pooled to perform genomic DNA extraction (Wizard® Genomic DNA Purification Kit, Promega) and subsequently do amplicon sequencing on the dsx target to check for the presence of mutations that could have prevented the homing ...
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bioRxiv - Plant Biology 2020Quote: ... the band of interest excised from the gel and cleaned using Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit and cloned into pGEM-T (Promega) for amplification before sequencing.