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Citations for Promega :
801 - 850 of 1589 citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... proteins were labeled during CFE with FluoroTect™ (Promega, Madison, Wisconsin, USA). FluoroTect™ was added to CFE reactions at 3.33% v/v ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were digested with 100 ng porcine sequencing grade modified trypsin (Promega) overnight at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were then digested with trypsin (Trypsin Gold, Mass Spectrometry Grade, Promega) using a 1:20 enzyme to sample ratio (w/w ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were digested using trypsin/Lys-C mixture (Mass Spec Grade, Promega) for 16 h according to the manufacture’s instruction ...
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bioRxiv - Cell Biology 2024Quote: ... protein samples were subjected to digestion with Trypsin/Lys-C Mix (Promega) at 47°C for 2 hours on S-Trap columns (ProtiFi) ...
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bioRxiv - Genomics 2022Quote: ADGRG6_3 and ADGRG_4 sequences were PCR amplified from human genomic DNA and cloned into the pGL4.23 plasmid (Promega, E8411). Human preadipocytes ...
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bioRxiv - Molecular Biology 2020Quote: ... The promoter area of human Rpl28 gene was cloned at HindIII/NcoI site of the pGL3-basic (Promega, E1751) to make pGL3-reporters ...
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bioRxiv - Genomics 2022Quote: Approximately 500-bp regions of DNA containing rs80282103 and rs11154336 were amplified from purified human genomic DNA (Promega, #G1521) by PCR using engineered restriction sites to allow directional cloning into the multiple cloning region of the pGL4.23[luc2/minP] luciferase reporter vector (Promega ...
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bioRxiv - Neuroscience 2021Quote: We constructed luciferase reporter plasmids by cloning an ∼900 bp region containing human 1b30 into the pGL4.24 vector (Promega) upstream of the minP ...
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bioRxiv - Microbiology 2023Quote: ... and Sp-EVs towards human endothelial cells was accessed by the CellTiter-Blue® (CTB) Cell Viability Assay (Promega), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... After 30 minutes of incubation at 37°C in 5% CO2 we added 50,000 ADCC-RL cells (Jurkat cell line expressing luciferase gene under the control of the NFAT response element and stably expressing human FcγRIIIa V158; Promega) in 50 µl RPMI 1640 medium with 6% low-IgG serum (Promega) ...
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bioRxiv - Neuroscience 2023Quote: ... We amplified the regions from C57Bl/6 tail snip DNA or from human male genomic DNA (Promega catalog # G1471) using FastPhusion 2x Master Mix (Thermo Fisher catalog # F548L) ...
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bioRxiv - Molecular Biology 2024Quote: ... and the equivalent region in the human (−207/+5) were cloned into pGL3-basic Luciferase reporter-vector (E1751, Promega) using the forward primer containing XhoI restriction site ...
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bioRxiv - Genomics 2023Quote: ... Reference allele enhancer sequences for hZRS and hs737 were obtained via PCR cloning from human genomic DNA (Promega, G304A). Primers used for each enhancer sequence are outlined in Table S2 ...
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bioRxiv - Cancer Biology 2023Quote: IDH1 and NNT promoter sequences were amplified from genomic DNA of human primary melanocytes by PCR and cloned into pGL4.12 (Promega). Mutagenesis of E-boxes was performed using a QuikChange Site-Directed Mutagenesis Kit (Stratagene) ...
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bioRxiv - Biophysics 2022Quote: ... pHalo-SMARCA4 expresses human SMARCA4 with HaloTag fused to the N-terminus under a CMVd1 promoter (Promega ORF FHC12075). pHalo-MED26 expresses human MED26 fused with a HaloTag at the N-terminus and was a kind gift from Joan Conaway’s lab ...
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bioRxiv - Cancer Biology 2022Quote: ... a 3kb region of the promoter containing HIC1 binding sites was amplified by PCR from human genomic DNA and cloned into the pGl4.26-luc vector (Promega); primers are listed in supplementary Table S1 ...
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bioRxiv - Molecular Biology 2022Quote: ... The human Gβ1 with a C-terminal 15-amino acid polypeptide linker was followed by a HiBiT (peptide 86, Promega), and the scFv16 was modified with an N-terminal GP67 signaling peptide and a C-terminal 8× histidine tag ...
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bioRxiv - Cell Biology 2023Quote: ... TTBK2 KO cell lines were seeded on glass coverslips (Matrigel-coated for hPSCs) and the next day transfected with 0.5μg TTBK1-HaloTag® human ORF in pFN21A (FHC12512, Promega) or pglap1-TTBK2 (“GFP-TTBK2” ...
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bioRxiv - Cell Biology 2024Quote: ... they were transfected with either control plasmid or Flag-GSK-3α WT human plasmid using Fugene 6 (Promega, #E2693) at a 3:1 ratio (DNA:Fugene 6) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cell surface levels of HiBiT-tagged human S1PR1 were monitored using a Nano Glo HiBiT Extracellular Detection System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2024Quote: Tracking was performed in Human Bronchial Epithelial Cells (HBEC) labeled with between 0.5 pM – 1 pM JF646 dye (Promega). At this concentration of dye ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested by 2 h Trypsin/Lys-C mix incubation (V5073, Promega) and collected ...
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bioRxiv - Cell Biology 2020Quote: ... Radiolabelled protein was translated from mRNA using the rabbit reticulocyte lysate system (Promega) and 35S-labelled methionine ...
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bioRxiv - Cell Biology 2020Quote: ... The protein was digested for overnight with 5 ng/µL trypsin (Promega Corporation) in 50 mM ammonium bicarbonate at 37 °C ...
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bioRxiv - Biophysics 2020Quote: ... The construct expresses rat GR fused with HaloTag protein (Promega, Madison, WI, USA) in the C-terminal domain under the CMVd1 promoter ...
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bioRxiv - Developmental Biology 2020Quote: Proteins were digested on-bead with 0.5 ug Sequencing Grade Modified Trypsin (Promega) overnight at 37°C with agitation ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were digested overnight with sequencing-grade porcine trypsin (Promega, Fitchburg, MA, USA).
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bioRxiv - Neuroscience 2021Quote: ... The proteins were digested with 2.5 μg of sequencing grade modified trypsin (Promega). The resulting peptides purified with C18 MicroSpin columns (The Nest Group ...
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bioRxiv - Cell Biology 2020Quote: ... before analysis of luciferase protein levels using Dual-Glo luciferase assay system (Promega) and an EnSpire multimode plate reader (Perkin-Elmer).
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bioRxiv - Systems Biology 2020Quote: The total resultant protein content was digested with trypsin (Trypsin Gold, Promega, USA). The enzyme was added at the ratio of 1:40 (w/w ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were directly digested on the beads by trypsin (Promega, Madison, WI, USA) overnight at 37°C in a 25-mM NH4HCO3 buffer (0.2 µg trypsin in 20 µL) ...
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S-nitrosoglutathione reductase deficiency causes aberrant placental S-nitrosylation and preeclampsiabioRxiv - Physiology 2021Quote: ... 500 μg was digested overnight using 0.02 μg trypsin/μg of protein (Promega). iodoTMT6 labeled samples were combined according to the label’s isotope ...
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bioRxiv - Plant Biology 2021Quote: ... Protein production was confirmed by western blot using an anti-HaloTag antibody (Promega). HaloTag-ligand conjugated magnetic beads (Promega ...
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bioRxiv - Biochemistry 2020Quote: ... One hundred μg of protein from each sample were digested with trypsin (Promega) at 37°C for 24 hours with an enzyme to protein ratio 1:100 (w/w) ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were digested with 0.4 µg of sequencing grade modified trypsin (Promega, #V5111) in 40 µl Hepes/NaOH ...
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bioRxiv - Plant Biology 2021Quote: ... The fusion proteins were purified using the MagneGST™ Pull-Down System (Promega). The subsequent EMSAs were performed using a LightShiftTM Chemiluminescent EMSA Kit (Thermo Fisher ...
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bioRxiv - Systems Biology 2021Quote: ... Proteins were then digested to peptides with sequencing grade modified trypsin (Promega V5113), at 37 °C overnight.
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bioRxiv - Cancer Biology 2020Quote: ... Protein samples were digested by 2 µg sequencing grade trypsin (Promega, Madison, WI) at 37°C with shaking overnight ...
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bioRxiv - Systems Biology 2022Quote: ... Protein enzymatic cleavage was carried out with trypsin (Promega; 1:20, w/w) at 37 °C for 16 h ...
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bioRxiv - Neuroscience 2022Quote: ... proteins were digested overnight at 37°C by the addition of trypsin (Promega) at a 1:100 enzyme:substrate ratio ...
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bioRxiv - Immunology 2022Quote: ... and IgG captured and eluted from Magne Protein A beads (Promega, Madison, WI) as previously described (35 ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were digested with 0.7 μg Trypsin/Lys-C Mix (MS grade, Promega) in 50 mM ammonium bicarbonate overnight at 37 °C ...
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bioRxiv - Biochemistry 2020Quote: ... ATP (0.2 mM) and/or protein kinase A (0.1 mg/mL; PKA, Promega) were then added to hydrogel droplets for 12 hours at 25 °C ...
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bioRxiv - Immunology 2021Quote: ... destained and protein digested overnight at 37°C with trypsin (Promega, Madison, Wisconsin). The digested peptide samples were desalted and concentrated using C18 zip tips (Eppendorf ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were digested at room temperature with 0.5 μg Lys-C (Promega, # VA1170) for 4 h ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were in gel digested with trypsin (Trypsin Gold, mass spectrometry grade, Promega) overnight at 37 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... proteins were extracted from cultured cells in passive lysis buffer (Promega, cat # E1941) containing protease inhibitors (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins from HL-1 cells were extracted with Passive Lysis Buffer (Promega France). Bioluminescence was quantified with a luminometer (Centro LB960 ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein enzymatic cleavage was carried out with trypsin (Promega; 1:50, w/w) at 37 °C for 16 h ...