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Citations for Promega :
1051 - 1100 of 1589 citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Air-dried protein pellets were solubilized using 8M urea and Protease Max surfactant (Promega, V2071), then digested with trypsin (Promega ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested overnight at 37 °C by addition of 1 µg of trypsin (Promega). Thereafter ...
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bioRxiv - Molecular Biology 2021Quote: ... and proteins were in-gel digested with trypsin (Promega, Madison, WI, USA; ratio 1:100) at 37°C overnight after de-staining ...
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bioRxiv - Molecular Biology 2021Quote: ... In-solution protein digestion was performed using Lys-C (Wako Chemicals, Neuss) and trypsin (Promega). For analysis of the input samples ...
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bioRxiv - Molecular Biology 2021Quote: ... chymotrypsin in a chymotrypsin/protein ratio of 1/20 (w/w) (Promega Cat. No. V1061) and incubated overnight at 25 °C or endoproteinase GluC in a GluC/protein ratio of 1/20 (w/w ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein pellets were resuspended in 50mM ammonium bicarbonate in the presence of 0.0015% ProteaseMAX (Promega) and total protein amount was estimated with Pierce BCA assays (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: Proteins on beads were digested overnight at 37°C with trypsin (Promega, Madison, WI, USA) in a 25-mM NH4HCO3 buffer (0.2µg trypsin in 20µL) ...
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bioRxiv - Microbiology 2020Quote: ... PacC222 and PacC80 proteins were individually expressed in the pGEX-4T-3 vector (Promega, USA) in E ...
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bioRxiv - Immunology 2021Quote: Tandem mass spectrometry was performed on the in-gel digested proteins with trypsin (Promega, Singapore), as described earlier (42) ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein samples were digested using 4 μg of trypsin (sequencing grade, low autolysis trypsin; Promega), at 37 °C for 16 h ...
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bioRxiv - Neuroscience 2021Quote: ... the proteins were digested with 0.2 µg of sequencing-grade trypsin (Promega, Madison, WI, USA) overnight at 37°C.
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bioRxiv - Plant Biology 2023Quote: ... Proteins were produced in vitro via the TNT SP6 Quick Coupled Transcription/Translation System (Promega) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were extracted by methanol-chloroform precipitation and digested with 1 μg of trypsin (Promega) in 100 mM EPPS (pH 8.0 ...
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bioRxiv - Microbiology 2023Quote: Reduced and alkylated proteins were in-gel digested with 100ng trypsin (modified sequencing grade, Promega) overnight at 37° C ...
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bioRxiv - Plant Biology 2023Quote: ... The proteins were resuspended in 100 mM NH4HCO3 and were digested with trypsin (Promega, UK) at an enzyme-to-substrate ratio of 1:25 ...
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bioRxiv - Bioengineering 2023Quote: ... 2.25 ng/uL Single-Stranded DNA Binding Protein (Sigma, cat# S3917 OR Promega, cat# M301A). 12.5 uL of master mix was mixed with 12.5 uL of 2x input oligonucleotide ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were precipitated with trichloroacetic acid and digested with Lys-C (Wako) and trypsin (Promega). The peptides were then analysed using an Ultimate 3000 nano-RSLC (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: Proteins for CoIP were produced with the TnT Quick Coupled Transcription/Translation System (Promega, www.promega.com) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... followed by overnight digestion with trypsin (enzyme to protein ratio of 1:100) (Promega, Germany) at 37 °C ...
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bioRxiv - Synthetic Biology 2023Quote: ... Bound protein was quantified using the Nano-Glo® HiBiT Extracellular Detection System (N2420, Promega). To quantify immobilized DNA in wells without protein ...
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bioRxiv - Genomics 2023Quote: Proteins were expressed in rabbit reticulocyte lysate (TNT T7 Quick Coupled Transcription/-Translation System, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: One-hundred μl protein expression reaction of TNT SP6 Coupled Reticulocyte Lysate System (Promega, L4600) containing 1000 ng pIXHALO-bZIP plasmids and 1500 ng pIXSBP-bZIP plasmids were incubated for 3 h at 30°C ...
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bioRxiv - Plant Biology 2022Quote: In vitro translation was carried out using a wheat germ protein expression system (Promega, USA) following the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2022Quote: ... Eluc activity and protein concentration in supernatant were measured using the Luciferase Assay System (Promega) and the Pierce 660 nm Protein Assay Reagent (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins captured on magnetic beads were digested with 1.5 mg/mL trypsin (Promega Cat# V5111) in 50 mM ammonium bicarbonate overnight at 37 °C ...
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bioRxiv - Plant Biology 2024Quote: ... Protein samples eluted with biotin were digested in-solution with 1 ug Trypsin/LysC (Promega) overnight at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... Protein detection was done using primary antibody anti-HaloTag monoclonal antibody (1:1,000 dilution, Promega), primary antibody anti-MamE polyclonal antibody (1:3,000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were resuspended in 100 mM NH4HCO3 and were digested with trypsin (Promega, UK) at an enzyme-to-substrate ratio of 1:25 ...
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bioRxiv - Cell Biology 2023Quote: ... Test proteins were generated in vitro using the TNR quick-coupled transcription-translation kit (Promega). For radiolabeling ...
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Arabidopsis actin-binding protein WLIM2A links PAMP-triggered immunity and cytoskeletal organizationbioRxiv - Plant Biology 2023Quote: ... Proteins were then digested overnight at 37°C with trypsin (Porcine trypsin, Promega, Fitchburg, WI). After stopping the digestion with 1% formic acid ...
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bioRxiv - Plant Biology 2024Quote: ... We further used the TNT SP6 High-Yield Wheat Germ Protein Expression System (L3260, Promega) to express the four TFs-HaloTag fusion protein ...
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bioRxiv - Molecular Biology 2023Quote: ... we labeled HALO-tagged49 proteins with HALO-Alexa488 or HALO-TMR dyes (Promega, G1001, G8251) by incubating the concentrated protein with dye for 1 hour on ice ...
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bioRxiv - Neuroscience 2023Quote: ... proteins on beads were digested with the addition of 2 µg trypsin (Promega, Cat# V511A) for 18 h at 37°C.
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bioRxiv - Plant Biology 2023Quote: Proteins were expressed using the TnT Coupled Wheat Germ Extract System with SP6 promoter (Promega). Protein expression was confirmed by Western Blot ...
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bioRxiv - Microbiology 2023Quote: ... for determination of viral RNA synthesis/protein expression or 50 µl CellTiter-Glo reagent (Promega) for determination of cell viability/ATP concentration ...
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bioRxiv - Developmental Biology 2022Quote: ... The 50μg of proteins were then trypsin digested overnight at 37 °C (1:25; Promega) using the filter aided sample preparation (FASP ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were digested by 0.2 μg modified grade porcine trypsin (Promega, Co., Madison, WI) during overnight incubation at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Protein synthesis was performed in the presence of [35S]methionine in rabbit reticulocyte lysate (Promega). Coupled translation/translocation reactions of 20 μl contained 16.5 μl of reticulocyte lysate ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were alkylated in-gel prior to digestion with 100ng trypsin (modified sequencing grade, Promega) overnight at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Protein pellets were resuspended in the digestion buffer as previously described and trypsin (Promega, UK) digested to peptides74 ...
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bioRxiv - Genetics 2022Quote: Proteins were produced in vitro via the TNT SP6 Quick Coupled Transcription/Translation System (Promega). If two proteins were to be tested together in a single binding reaction ...
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bioRxiv - Cell Biology 2023Quote: ... and finally radiolabelled precursor proteins were synthesized with the Flexi rabbit reticulocyte lysate system (Promega). 35S-radiolabeled precursor proteins were incubated with isolated mitochondria in import buffer (3% (w/v ...
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bioRxiv - Biochemistry 2023Quote: Proteins labeled with [35S]-methionine were produced by coupled transcription and translation reactions (T7, Promega). Translation products were partially purified by ammonium sulfate precipitation [87] ...
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bioRxiv - Biochemistry 2023Quote: ... coli and HeLa cells as well as of a commercially available yeast protein digest (Promega), The ratios of the peptide amount of each species within the three mixtures are given in Table S1.
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bioRxiv - Neuroscience 2023Quote: ... Proteins were digested overnight at 37° C with 1 μg mass spectrometry grade Trypsin (Promega). The resulting peptide sample was acidified with 10% formic acid and 10% trifluoroacetic acid (TFA ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins on the beads were digested by adding 200 ng trypsin/Lys-C mix (Promega) at 37 °C overnight ...
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bioRxiv - Microbiology 2024Quote: ... In-gel protein digestion was performed overnight at 37°C with sequencing grade trypsin (Promega) in a protease:protein ratio of 1:50 (w/w) ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were digested with a solution of 0.05 μg/μL of trypsin/Lys-C (Promega) in 100 μL of AMBIC overnight at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... cells transfected with HALO-fusion protein were supplemented with 100 nM TMR HALO ligand (Promega). Cells were fixed at RT for 20 min in 3.7% formaldehyde (FA ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were digested by adding 5 μL of sequencing grade trypsin (0.1 mg/mL) (Promega) for three h at 37 °C ...