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Citations for Promega :
801 - 850 of 1012 citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... before treatment without or with OSM (10 ng/ml, 45 min) and RNA extraction (ReliaPrep RNA Cell Miniprep System, Promega). cDNA was generated using oligo(dT)20 primer (GoScript Reverse Transcription System ...
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bioRxiv - Microbiology 2020Quote: ... the plate was incubated at room temperature for 10 minutes to stabilize luminescent signal before luminescence was recorded on a GloMax plate reader (Promega).
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bioRxiv - Microbiology 2021Quote: ... Protein samples were diluted 10-fold in 50 mM Tris-HCl and digested with Sequencing Grade Modified Trypsin (Promega - V5111) at a Protein:Trypsin ratio 50:1 overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR mixture was at a volume of 10 μL containing 5 μL SYBR Premix Ex Taq (Promega, Madison, USA), 0.5 μM each of the primers ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... at room temperature, with 40 μL assay buffer (CO2-independent medium, 10% FBS) supplemented with 2% (v/v) GlosensorTM reagent (Promega). To stimulate endogenous cAMP via β adrenergic-Gs activation ...
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bioRxiv - Microbiology 2021Quote: ... mice were anesthetized with ketamine-xylazine (100 mg/kg ketamine and 10 mg/kg xylazine) and then retro-orbitally injected with 100 µl of Nano-Glo luciferase substrate (Promega) diluted 1:10 in PBS ...
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bioRxiv - Plant Biology 2020Quote: ... The concentrations of the resulting cDNAs were adjusted to 10 ng/µL and 2 µL were used for RT-qPCR reactions performed with the GoTaq qPCR Master Mix (Promega) in a final volume of 10 µL ...
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bioRxiv - Microbiology 2020Quote: ... at 9 h post-electroporation and luciferase activity was measured in 10 µl aliquots of the cell lysates using a Luciferase Reporter Assay System (Promega).
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bioRxiv - Cell Biology 2021Quote: ... the antibody–bead mixture was resuspended in 500 μl of the reaction mixture containing 10 μg of fragmented total RNA,100 μl of reaction buffer and 5 μl of RNasin Plus RNase Inhibitor (Promega), and incubated for at least 4 h at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... gel pieces were reswelled in 60 μL of 50 mM NH4HCO3 buffer containing 10 ng/μL of trypsin (modified porcine trypsin sequence grade, Promega) incubated for one hour at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... 70 μL of PBS were added to each well followed by addition of 10 μL of a 50 μM coelenterazine-h solution (Promega). After 10-min incubation ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were lysed for 15 minutes on ice then treated with 10 µL of RQ1 DNase (Promega, catalog no. M610A) for 5 minutes at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... Cell lines were validated by short tandem repeat (STR) DNA typing using the Promega GenePrint 10 System according to the manufacturer’s instructions (Promega #B9510). Cells were cryopreserved in Recovery Cell Culture Freezing Medium (Gibco #12648-010) ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 μl of the lysate was used to measure luciferase activity by sequential mixing with 10 μl of each substrate from the Dual Luciferase Reporter Assay System (Promega). Luminiscence was measured on a Mithras LB 940 microplate reader (Berthold Technologies) ...
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bioRxiv - Microbiology 2022Quote: ... and the Gaussia princeps luciferase enzymatic activity was measured on a Centro XS LB960 microplate luminometer (Berthold Technologies, reading time 10 s after injection of 50 µl Renilla luciferase reagent (Promega). For the minigenome assays ...
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bioRxiv - Neuroscience 2020Quote: ... the culture medium was replaced with a CO2-independent medium containing 10% (vol/vol) FBS and 2% (vol/vol) GloSensor cGMP stock solution (Promega). The cells were then incubated for 2 h at 27°C in the dark ...
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bioRxiv - Plant Biology 2021Quote: ... a 10 mm × 10 mm piece of tissue surrounding the inoculation point was applied to the Dual-Luciferase Reporter Assay System (Promega), to detect the firefly luciferase activity (or activation of the ACS2 and ACS2mut promoters) ...
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bioRxiv - Microbiology 2021Quote: ... air-dried and treated by 10 mkl of 12 mg/mL solution of trypsin (Trypsin Gold, Mass Spectrometry Grade, Promega) in 50 mM ammonium bicarbonate for 15 h at 37°C ...
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bioRxiv - Plant Biology 2021Quote: ... The proteins were then incubated for 30 min on ice in the presence of 10 μL of 20 ng/μL trypsin (Trypsin V5280, Promega), followed by 16 h at 37 °C ...
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bioRxiv - Plant Biology 2021Quote: ... Residual genomic DNA was then removed from each sample with 10 U of RNAse-free DNaseI (Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... cells were mock treated or treated with 10 unit/mL of uIFN for 6 hrs and collected in 1× passive lysis buffer (Promega). Lysates were assayed for luciferase activity using the Dual Luciferase Reporter Assay System (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... and the proteins were on-bead digested using 5 µl of Sequencing Grade Trypsin (100 ng/µl in 10 mM HCl, Promega). The digestion was carried out at 37°C over night ...
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bioRxiv - Microbiology 2021Quote: ... Five ml of BHI broth containing 10 μg/ml tetracycline was inoculated with a single colony and genomic DNA was extracted (Wizard DNA extraction kit, Promega). Genomic DNA was sequenced by paired-end joining Illumina (Biomics Platform of the Institut Pasteur ...
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bioRxiv - Cancer Biology 2021Quote: ... The reaction mixture was then transformed at a ratio of 1:10 (reaction volume/volume competent cells) into HB101 competent cells (Promega). Spectinomycin-resistant colonies were screened by restriction digestion of miniprep DNA and subsequently validated by DNA sequencing ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 μL of the annealed oligos were ligated with 0.04 ng digested PspCAs13b crRNA backbone in 10 μL of T4 ligation buffer (3 h, RT) (Promega, M1801). All PspCas13b crRNA spacer sequences used in this study are listed in Supplementary File 1 ...
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bioRxiv - Plant Biology 2022Quote: ... 100 ng total DNA was added to a 20 μl PCR containing 10 μl GoTaq® Master Mixes (Promega, USA) and 0.1 μM of each primer pair ...
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bioRxiv - Cancer Biology 2022Quote: ... Ten to twenty 10 μm cryosections were used for nucleic acid extraction using the automated Maxwell® RSC instrument (Promega) with the appropriate sample kit and according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... and activity of both luciferases was measured in 10 μL of the sample with the Dual Luciferase Reporter Assay system (Promega) on a Modulus Single Tube Reader (Turner Biosystems) ...
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bioRxiv - Plant Biology 2021Quote: ... Treatment with Proteinase K was carried out in 500 μl of PK Buffer (100 mM Tris-HCl pH8; 50 mM NaCl; 10 mM EDTA; Proteinase K 4 mg/ml; RNasin PROMEGA) 2 h at 55 °C and 15 min at 95 °C ...
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bioRxiv - Plant Biology 2021Quote: ... Three washes were performed with ChIP Dilution Buffer as in DRIP assays before incubating the IP fraction with Proteinase K for 2 h at 55°C plus 15 min at 95°C in PK Buffer (100 mM Tris-HCl pH8; 50 mM NaCl; 10 mM EDTA; 4 mg/ml Proteinase K; RNasin PROMEGA). We added 2 U DNase I (ThermoFisher ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 40 μl of the cell suspension were transferred to each well of 96-well plates containing 10 μl of 5X GloSensor cAMP Reagent (Promega) prepared according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... protein lanes were cut into 10 pieces, destained (600 rpm, 37 °C, 200 mM NH4HCO3 in 30% acetonitrile) and digested with trypsin (sequencing grade, Promega) overnight at 37 °C ...
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bioRxiv - Microbiology 2021Quote: PS cells were seeded at 1.5 × 105 cells per well in 96-well plates (n=3) and transfected with in vitro transcribed TBEV replicon RNA (100 ng/well) and 10 ng of pTK-Ren (Promega) using Lipofectamine-2000 (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... was added to each well and the plate was incubated for 10 minutes at room temperature before luminescence was measured using a GloMAX Discover Plate reader luminometer (Promega). Three replicate experiments were performed with three biological replicates for each cell type and treatment within each experiment for nine total measurements per cell type and treatment.
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bioRxiv - Immunology 2021Quote: We seeded 1 × 105 of HEK293 cells into 24-well plates in triplicate and transfected the cells with an expression vector together with p-125 Luc reporter (100 ng/well) and phRL-TK (10 ng/well, Promega) plasmids ...
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bioRxiv - Immunology 2020Quote: ... HF and MEF cell lines were transfected with 10 ng of the internal control plasmid pTK-Renilla (pRL-TK, Promega) or 60 ng of the reporter plasmid pLUC-NF-κB (R ...
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bioRxiv - Neuroscience 2021Quote: ... or methadone were added for another 10 min followed by addition of 20 μL of the RLuc substrate coelenterazine h (Promega), at 5 μM final concentration ...
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bioRxiv - Cancer Biology 2021Quote: ... The cell pellet was suspended in buffer P1/RNAse A and lysed by adding 1/20 volume of 10% SDS (Promega). After incubating for 20 min at room temperature ...
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bioRxiv - Plant Biology 2021Quote: ... The DNA pellet was resuspended in water with RNase A (10 µg/mL) for amplicon library preparation using GoTaq G2 Green Master Mix (Promega) and primers (Supplemental Table S1 ...
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bioRxiv - Cell Biology 2021Quote: ... 50°C for 60 min and 70°C for 15 min) in the presence of 10 fg of Luciferase RNA (Promega) using the SuperSript III reverse transcription kit (Invitrogen) ...
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bioRxiv - Plant Biology 2020Quote: ... The luciferase assay was performed by thoroughly mixing 10 μl of a sample with 50 μl of the luciferin substrate (Luciferase Assay System, Promega) and measuring the luminescence intensity after 10 and 20 seconds using a luminometer (TD-20/20 Luminometer ...
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bioRxiv - Immunology 2021Quote: ... Fluorescence of FITC-dextran in serum was diluted to 1:10 with PBS and measured on a Promega GloMax Multi Detection System (Promega) at 490 nm excitation and 520 nm emission wavelengths ...
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bioRxiv - Cell Biology 2021Quote: ... Beyotime) for 10 min at 37°C and the other was treated with 1 U/ul RNase inhibitor (N2518, Promega). For chromatin extraction ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were on-bead digested using 5 μl of Sequencing Grade Trypsin (100 ng/μl in 10 mM HCl, Promega). The digestion was carried out in a microwave instrument (Discover System ...
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bioRxiv - Biochemistry 2022Quote: ... 1.7 μg of attB-hACE2-mNG2-1-10 plasmid or attB-S-mNG2-11 plasmid were added into 5 μL FuGENE 6 transfection reagent (Promega) and OptiMEM (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were washed 3 times for 10 min in TBST and subsequently incubated with species-specific HRP-conjugated secondary antibodies (1:10000; Promega) in 5% non-fat dry milk -TBST for 1 h at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... Rescue assays were performed in media supplemented with 10%(v/v) FBS as previously described using Cell Titer 96 MTS (Promega). Briefly ...
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bioRxiv - Plant Biology 2022Quote: ... The primer and DNA marker were end-labeled with 10 μCi of [γ-32P]ATP by T4 polynucleotide kinase (Promega) and then used for reverse transcription with avian myeloblastosis virus (AMV ...
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bioRxiv - Microbiology 2022Quote: ... at 72° C for 10 min to add 3’-A overhangs for T/A cloning with pGEM-T Easy plasmid (Promega). A 610 bp DNA fragment upstream of flgV was amplified using PCR primers 85 and 86 ...
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bioRxiv - Microbiology 2022Quote: ... A 5 mL sample was then mixed with 10 mL of RNAprotect and processed with the SV Total RNA Isolation System (Promega) according to the manufacturer’s instructions ...