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Citations for Promega :
701 - 750 of 1012 citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... we supplemented culture medium with 10 μL of CellTiter96 Aqueous Non-Radioactive Cell Proliferation Assay (MTS) kit (Promega) reagent for 1 hour ...
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bioRxiv - Cell Biology 2024Quote: ... Solvent was removed in a vacuum centrifuge and 100 µl 10 ng/µl sequencing grade Trypsin (Promega, US) in 10 % ACN ...
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bioRxiv - Genetics 2024Quote: ... 1 μL cDNA was used in 10-μL reactions containing Brilliant III SYBR Green qPCR Master Mix (Promega). Biological replicates were analyzed as duplicates ...
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bioRxiv - Cancer Biology 2024Quote: ... The cell identity was confirmed through K562 STR DNA fingerprinting (https://www.atcc.org/products/ccl-243) by GenePrint 10 System Kit (Promega).
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bioRxiv - Microbiology 2023Quote: ... The concentrated phages were next treated for 30 min at 37°C with 10 µl of DNAse (Promega) and 2.5 µl of RNAse (Macherey-Nagel ...
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bioRxiv - Microbiology 2023Quote: Total DNA was extracted from 10 ml bacterial overnight grown cultures using Wizard Genomic DNA Purification Kit (Promega) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: A total of 10 µg RNA was taken from each sample for reverse transcription (RT) using GoScript (Promega) reverse transcriptase (5 µg for RT ...
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bioRxiv - Genomics 2023Quote: ... 1 μL of each primer (10 μM) and 12.5 μL of GoTaq Green Master mix (Promega®, USA). The PCR was run in a thermocycler (BioRad ...
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bioRxiv - Cancer Biology 2023Quote: ... and cell culture identity verification was done by short tandem repeat profiling (GenePrint® 10 System, Promega #B9510).
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MET functions in tumour progression and therapy resistance are repressed by intronic polyadenylationbioRxiv - Molecular Biology 2023Quote: ... PCR reactions were performed using 10 ng of cDNAs (RNA equivalent) and the GoTaq DNA polymerase (Promega, # M8295). For each gene ...
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bioRxiv - Microbiology 2023Quote: Cell supernatants were collected and stored in LDH storage buffer + Triton 10% as per the manufacturer’s instructions (Promega). Supernatants were inactivated for 2 hours at room temperature prior to usage outside the BSL-3 ...
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bioRxiv - Developmental Biology 2023Quote: ... modified hypotonic lysis buffer with a 10-fold higher concentration of Cycloheximide and Recombinant RNasin Ribonuclease Inhibitor (Promega) was used instead of RNase OUT ...
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bioRxiv - Cell Biology 2023Quote: ... A 90 μl volume of each culture and 10 μl of the furimazine (1 in 200 dilution; Promega) were incubated in a Cellstar non-transparent white 96-well microplate for one h and the bioluminescence intensity was monitored with a Tecan Infinite 200 PRO plate reader every 30 s ...
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bioRxiv - Genetics 2024Quote: ... The microinjection reagents were combined in nuclease-free IDT TE pH 7.5 (Integrated DNA Technologies; Tris 10 mM, EDTA 0.1 mM) supplemented with Rnasin (Promega) at 0.2 U/μl ...
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bioRxiv - Genetics 2024Quote: ... gel slices were soaked in 50 mM TEAB containing 10 ng/µL sequencing-grade trypsin (Promega, Southampton, UK) and incubated at 50 °C for 8 h ...
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bioRxiv - Immunology 2024Quote: ... 10 µL of the total RNA was used to produce cDNA with the GoScript Reverse Transcription Mix (Promega) following manufacturer’s recommendation ...
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bioRxiv - Molecular Biology 2024Quote: ... 25 mM potassium phosphate pH 7.2) and digested initiated by adding 10 μL of 0.1 mg/mL GluC protease (Promega). Digestion continued overnight at 37 °C ...
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bioRxiv - Plant Biology 2024Quote: 10 nmol of Halo-tagged MpCESAR CUE domain was incubated with 100 μl of the HaloLink resin (Promega) in 500 μl of the coupling buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 μL of a mixture of 10 mM dNTPs and 200 U of M-MLV reverse-transcriptase (Promega) were added to the mixture and incubated at 37 °C for 60 minutes ...
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bioRxiv - Genetics 2024Quote: ... PCR reactions were carried out in 10 µL-volumes containing 1x GoTaq® reaction buffer (Promega, Southampton, UK), 0.1 mM dNTPs ...
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bioRxiv - Developmental Biology 2024Quote: ... The samples were further diluted to 0.5 M guanidine with 10 mM EPPS pH 8.5 and Trypsin protease (Sequencing Grade Modified Trypsin, Promega) was added to a final concentration of 10 ng/mL along with an additional 20 ng/mL of LysC ...
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bioRxiv - Cell Biology 2020Quote: ... Trypsin digestion was performed by addition of 30 µL of 10 ng/µL sequencing grade trypsin (Promega, Madison, WI) in AmBic followed by 30 µL of additional AmBic ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was diluted either 1:5 or 1:10 for all qPCR experiments and GoTaq qPCR Master Mix (Promega) was used to amplify cDNA ...
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bioRxiv - Molecular Biology 2021Quote: ... were added to the 5′ end labelled 187cspA RNA in a total volume of 10 µL in Buffer O containing 0.4 U/µL of RNasin (Promega). Complex formation was performed at 15°C or 37°C for 15 min ...
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bioRxiv - Molecular Biology 2021Quote: ... the samples were incubated at either 15°C or 37°C for 10 min in Buffer I containing 0.4 U/µL of RNasin (Promega). Following renaturation ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μL of stopped reactions were added to 50 µL of luciferase substrate solution (Luciferase Assay System kit, Promega) in a 96-well white microplate ...
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bioRxiv - Molecular Biology 2021Quote: ... 11 μL of substrate mix (10 μL of Promega Nano-Glo® Luciferase Assay Buffer, 0.1 μL of Promega NanoLuc® luciferase substrate and 1 μL of 10 mg/mL CHX ...
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bioRxiv - Immunology 2021Quote: ... Single CD3-CD8-CD14-CD16-CD20+Ova-RBD-PE+RBD-AF647+ B cells were sorted into individual wells of 96-well plates containing 4 μl of lysis buffer (0.5× PBS, 10 mM DTT, 3,000 units/ml RNasin Ribonuclease Inhibitors (Promega, N2615) per well using a FACS Aria III and FACSDiva software (Becton Dickinson ...
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bioRxiv - Plant Biology 2020Quote: ... 10 mg/L BSA and 1 mM PMSF) and assayed using the Dual-Luciferase® Reporter Assay System (Promega). Experiments were normalized to negative control set at 1.
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bioRxiv - Synthetic Biology 2021Quote: ... 1 µl of this crude lysate was used for PCR in a 10 µl GoTaq Green reaction (Promega M7123) with 0.25 µM of primers ...
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bioRxiv - Microbiology 2021Quote: IL-18 truncations were generated through in vitro transcription/translation using pET-pro-IL-18 as a template in 10 µl reaction volumes following the manufacturer’s recommendations (TNT Coupled Reticulocyte Lysate; Promega) as previously [40] ...
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bioRxiv - Neuroscience 2020Quote: cDNA synthesis was performed in a reaction volume of 25 μl (10 cell pools) or 20 μl (single cells) containing dNTPs (0.5 mM, Promega), random primers (100 ng/per tube ...
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bioRxiv - Biochemistry 2020Quote: ... the samples were brought to room temperature for 5-10 minutes while 2 mL of 30 mM coelenterazine (Promega), or 15 mL of 1-to-50 diluted NanoLuc substrate (Promega) ...
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bioRxiv - Cell Biology 2021Quote: ... Each PCR reaction was performed in a 10 μL mixture containing 0.25 U GoTaq G2 Hot Start Polymerase (Promega), 1x GoTaq G2 Buffer ...
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bioRxiv - Synthetic Biology 2021Quote: ... 10 μL of stopped reactions were added to 50 µL of luciferase substrate solution (Luciferase Assay System kit, Promega) in a 96-well white flat bottom microplate ...
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bioRxiv - Systems Biology 2020Quote: ... Samples were further diluted to 0.5 M GuHCl with 10 mM EPPS pH 8.0 and digested with an additional 10 ng/μL LysC and 20 ng/μL sequencing grade Trypsin (Promega) at 37 °C for 16 hours.
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bioRxiv - Microbiology 2020Quote: ... In vitro transcription/translation was performed with the corresponding primers in Table 1 using pET-pro-IL-1β as a template and following the manufacturer’s recommendations in 10 µl reaction volumes (TNT Coupled Reticulocyte Lysate; Promega). Loading for IL-1R reporter assays was normalized by total IL-1β product measured by enzyme-linked immunosorbent assay (R&D Systems).
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bioRxiv - Immunology 2022Quote: ... Single CD3−CD8−CD14−CD16−CD20+Ova−Gamma NTD-PE+Wuhan-Hu-1 NTD-AF647+ B cells were sorted into individual wells of 96-well plates containing 4 μl of lysis buffer (0.5× PBS, 10 mM DTT, 3,000 units/ml RNasin Ribonuclease Inhibitors (Promega, N2615) per well using a FACS Aria III and FACSDiva software (Becton Dickinson ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were further diluted to 0.5 M GuaCl with 10 M EPPS pH 8.5 and digested with an additional 10 ng/µL LysC and 20 ng/µL sequencing grade Trypsin (Promega) at 37°C for 16 h ...
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bioRxiv - Microbiology 2022Quote: ... Samples were digested by adding 100 µL trypsin solution (10 mM TEAB, pH 7.5, 2 µg Promega trypsin #V5111) and incubated overnight at 37 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines were cultured in Dulbecco’s modified Eagle medium (DMEM) (HyClone) with 10% fetal bovine serum FBS (HyClone) and 1% penicillin-streptomycin (P.S.) (Promega) at 37 °C in a 5% CO2 atmosphere.
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bioRxiv - Microbiology 2020Quote: ... The proteins in the sample were digested with 10 μg/mL sequence grade modified trypsin (Promega, Madison, WI, USA) at 37°C overnight ...
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Herpes simplex virus 1 entry glycoproteins form stable complexes prior to and during membrane fusionbioRxiv - Microbiology 2022Quote: ... the media of the effector cells was replaced with 40 µl per well of fusion medium (Ham’s F12 with 10% FBS, Penicillin/Streptomycin, 50 mM HEPES) with 1:50 Endurazine luciferase substrate (Promega) added ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were treated with the indicated drugs in combination with pan-caspase inhibitor Z-VAD-FMK (10 mM, Promega). After 24 hours ...
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bioRxiv - Genomics 2022Quote: ... 2 µl of yeast lysate was used as a template in a 10 µl GoTaq Green reaction (Promega M7123) with 0.25 µM of primers ...
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bioRxiv - Synthetic Biology 2020Quote: ... at 54°C for 10 min and the resulting cDNA amplified with GoTaq Flexi DNA polymerase (Promega; Madison, WI) for 8 PCR cycles in the same reaction with a final volume of 100 μL ...
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bioRxiv - Plant Biology 2021Quote: ... Purified nucleus were recovered in 300 μl of Nuclei Lysis Buffer (50 mM Tris-HCl pH8; 0.1% SDS; 10 mM EDTA; 100 μM PMSF; RNasin PROMEGA) and 5 cycles (30 s ON – 30 s OFF ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were diluted 10-fold with 50 mM HEPES pH 8 and incubated with sequence-grade modified Trypsin (Promega) at 1/50 trypsin/protein ratio for 16 h at 37 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... STR profiling was carried out by PCR amplification of nine STR loci plus amelogenin (GenePrint 10 System; Promega, #B9510), fragment analysis (3730XL DNA Analyzer ...
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bioRxiv - Immunology 2021Quote: ... Single CD3−CD8−CD14−CD16−CD20+Ova−RBD-PE+RBD-AF647+ B cells were sorted into individual wells of 96-well plates containing 4 μl of lysis buffer (0.5× PBS, 10 mM DTT, 3,000 units/ml RNasin Ribonuclease Inhibitors (Promega, N2615) per well using a FACS Aria III and FACSDiva software (Becton Dickinson ...