Labshake search
Citations for Promega :
801 - 850 of 4110 citations for 2'3' cyclic GAMP cGAMP ELISA Kit 384 well Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: The “ADP-Glo” kit (Promega, Wisconsin) was used to measure hydrolysis of ATP to ADP and inorganic phosphate by ATPase ...
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bioRxiv - Bioengineering 2020Quote: ... Steady-Go luciferase assay kit (Promega) was used to measure the expression of luciferase in cells ...
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bioRxiv - Molecular Biology 2021Quote: ... a kit (Promega, Fitchburg, WI, USA) was used to perform TUNEL staining in accordance with manufacturer’s instructions.
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bioRxiv - Genetics 2020Quote: ... using GoTaq qPCR kit (Promega A6001). Plates were sealed using a microseal ‘B’ Adhesive Seal (Bio-Rad MSB1001 ...
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bioRxiv - Biochemistry 2020Quote: ... Using PureYield Plasmid Midiprep kit (Promega), DNA preparations of selected clones were subjected to sequencing at Eurofins Genomics Canada.
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bioRxiv - Cell Biology 2021Quote: ... with a HiBiT assay kit (Promega).
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bioRxiv - Microbiology 2021Quote: ... The Luciferase Assay System kit (Promega) was used to measure the luciferase activity in sporozoite samples following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... and NADP+/NADPH-Glo kits (Promega), respectively ...
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bioRxiv - Evolutionary Biology 2022Quote: ... or Wizard PCR purification kit (Promega), and then directly sequenced using the same and internal primers on an ABI 3130xl sequencer.
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bioRxiv - Molecular Biology 2022Quote: ... GoScript Reverse Transcription Kit (Promega, #A5003) was used as per manufacturer instructions with the following modifications ...
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bioRxiv - Microbiology 2023Quote: One step RT-PCR kit (Promega) was used ...
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bioRxiv - Cancer Biology 2023Quote: ... using SIRT-Glo Assay kit (Promega), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... as per the kit’s instructions (Promega), 75,000 mouse Fcγ receptor IV (mFcγRIV)-expressing Jurkat effector cells were added to each well to engage with the Fc region of M2e serum antibodies bound to native M2e expressed on the surface of the infected cells ...
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bioRxiv - Bioengineering 2023Quote: The NanoGlo luciferase assay kit (Promega) was used to determine the amount of functional Nanoluc protein in lysed biological samples by supplying the enzyme with substrate and immediately measuring the resulting luminescence ...
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bioRxiv - Cell Biology 2023Quote: ... Dual-Luciferase Assay Kit (E2920, Promega) was used to measure the dual-luciferase activity according to the manufacturer’s instruction.
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bioRxiv - Plant Biology 2023Quote: ... RNA of roots and nodule primordia was extracted using a TRIzol (Invitrogen)-adapted ReliaPrep RNA extraction kit (Promega), as previously described (53) ...
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bioRxiv - Plant Biology 2023Quote: ... RNA was extracted using a TRIzol (Invitrogen)-adapted ReliaPrep RNA extraction kit (Promega) [39] ...
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bioRxiv - Developmental Biology 2023Quote: ... and the Reverse transcription kit (Promega) was used to make cDNA following manufacturer indications ...
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bioRxiv - Immunology 2024Quote: ... The dual luciferase assay kit (Promega) was used to determine the luciferase activity ...
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bioRxiv - Cell Biology 2020Quote: ... 250 ng of each of the donor and Cas9-sgRNA vectors were transfected to RPE-1 cells cultured in 6 wells with 1.5 μl of FuGENE® HD (Promega, E2311) overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... as well as Tagged-BAF15 were synthesized using the TNT coupled reticulocyte lysate system according to the manual (Promega, L5020, USA). Briefly ...
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bioRxiv - Cancer Biology 2020Quote: ... 25uL of reconstituted CellTiter-Glo Luminescent Cell Viability reagent was added to each well and incubated for 5 minutes (Promega, G7571). Luminescence readings were taken using the BioTek Synergy H1 Hybrid Reader ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were added to each well and co-cultured for 16-20 hr before addition of Bright-Glo™ (Promega) lysis buffer and luciferin substrate ...
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bioRxiv - Microbiology 2021Quote: ... was added to each well and incubated for 1 h at RT before fluorescence intensity was read on a Glomax® Explorer Fully Loaded (Promega) with emission wavelengths of 500-550 nm and an excitation wavelength of 475 nm and graphs were generated using GraphPad Prism (V.8.4.1) ...
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bioRxiv - Cell Biology 2021Quote: Apoptotic cell death was measured in a 96-well format using the RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay (Promega) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... transfection experiments) or 4×105 cells (12 well format; IFN experiments) were lysed in 120 μl passive lysis buffer (Promega #E1941). Half the lysate was used to quantify luciferase protein (Promega Luciferase Assay System #E1501 ...
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bioRxiv - Microbiology 2021Quote: ... were added to 384-well plate wells containing 10 μM of each compound and were incubated with 10 μL of Nano-Glo luciferase assay reagent (Promega Corporation) at 22°C for 10 min and then read using a Perkin-Elmer Enspire plate reader at 460 nm ...
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bioRxiv - Microbiology 2022Quote: ... Cell viability was measured 72 hours post-infection by adding 100μl of CellTiter-Glo reagent to each well as described in the manufacturers protocol (CellTiter-Glo Luminescent Cell Viability Assay, Promega # G7571). Luminescence was quantified using the Infinite M200Pro TECAN apparatus.
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bioRxiv - Cancer Biology 2019Quote: Human SCLC cell lines plated in a 96-well microplate at a concentration of 5×103 cells/well were used to determine cell viability after 72h of incubation using CellTiter 96® AQueous One System (Promega). Epoch microplate spectrophotometer (Biotek ...
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bioRxiv - Developmental Biology 2022Quote: ... for the duration of imaging (approximately 2 minutes per embryo) with the yolk supported in a shallow well of solidified 1% low-melting agarose (Promega, V2111). Adult fish were photographed using a Panasonic DMC GX7 camera with a Panasonic Lumix G 20mm pancake lens ...
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bioRxiv - Genomics 2022Quote: ... media was gently removed from the wells by pipetting and cell luminescence was measured using the Dual-Glo assay (Promega E2920). Two luminescence normalizations were performed ...
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bioRxiv - Cancer Biology 2022Quote: ... Gaussia princeps luciferase enzymatic activity was measured using a Berthold Centro LB960 luminometer by injecting 50 µl per well of luciferase substrate reagent (Promega, E2820) and counting luminescence for 10 seconds ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 μL of freshly-thawed and prepared CellTiter-Glo® reagent was added to each well and mixed according to the manufacturer’s instructions (CellTiter-Glo®, Promega) to induce cell lysis ...
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bioRxiv - Genomics 2022Quote: ... cells were lysed and Renilla as well as Firefly Luciferase activity was measured using the Dual-Glo Luciferase Assay System (Promega, E2920) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Loss of cell viability reflecting the efficiency of viral infection was measured 3 days after infection by adding 100 μL Cell Titer-Glo reagent to each well (CellTiterGlo Luminescent Cell Viability Assay, Promega #G7571), according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... media was removed and replaced with 50 µL per well 0.01 mM Furimazine or NanoGlo® Lytic Buffer (diluted 1 in 500, Promega, UK). After a 10-minute shaking incubation protected from light ...
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bioRxiv - Immunology 2022Quote: ... together with Jurkat cells (90,000 cells per well) stably transfected with mouse FcγRIV and NFAT-REluc2 expression plasmids (Promega, Madison, WI, USA). After incubation at 37°C for 6 hours ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 4 h later 5 µl/well of NanoBRET™ Nano-GloR Substrate (10 µl/ml in DMEM no phenol red, Promega) was added and 460 nm donor and 618 nm acceptor signals were read within 10 min of substrate addition using CLARIOstar microplate reader (Mandel) ...
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bioRxiv - Immunology 2023Quote: ... 25 ng of c-DNA was used per reaction in each well containing the 2X SYBR green PCR master mix (Promega, USA) along with appropriate primers ...
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bioRxiv - Microbiology 2023Quote: ... 2 μL of furimazine substrate was added to the reaction well from a working reagent stock of a 2:100 NanoDLR Stop & Glo Substrate to Buffer ratio (Promega #N1610). A total well volume of approximately 112.5 μL was incubated at room temperature for 2 minutes prior to measuring luminescence and OD600 readings on an EnVision plate reader (PerkinElmer).
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bioRxiv - Immunology 2023Quote: ... 25 ng of c-DNA was used per reaction in each well containing the 2X SYBR green PCR master mix (Promega, USA) along with appropriate primers ...
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bioRxiv - Cell Biology 2024Quote: ... pre-amplified cDNA libraries were diluted with addition of 9µL water to each well before quantification using QuantiFluor® dsDNA System (Promega, #E2671) and normalisation and dilution to 100pg ul-1.
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bioRxiv - Cancer Biology 2021Quote: ... The 96-well plate was then allowed to equilibrate at room temperature for 30 minutes followed by addition of Cell Titer Glo (Promega) to each well according to manufacturer instructions ...
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bioRxiv - Neuroscience 2021Quote: ... coated 12-well plate and transfected the next day using the Fugene 6 transfection reagent and according to the manufacturer’s directions (Promega E2691). The same amount of plasmid was transfected per condition ...
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bioRxiv - Molecular Biology 2021Quote: ... Cytotoxicity on mock infected plates was determined using the Promega CellTiter-Glow Luminescent Cell Viability Assay (Promega, Madison, Wisconsin, USA). Half-maximal inhibitory concentration (IC50 ...
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bioRxiv - Biophysics 2022Quote: ... The reaction was further incubated for 60 min at room temperature prior to measuring absorbance with a plate-reading luminometer (GloMax, Promega). Samples were correlated to phosphate concentration standard controls ...
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bioRxiv - Microbiology 2021Quote: ... NF54-HGL parasites were treated with 3× IC50 for two weeks in two independent experiments and cultures were monitored by luminescence readout on the BioTek Synergy 2 Plate reader using ONE-Glo reagent (Promega). Whole-genome sequencing was performed to test for single nucleotide polymorphisms (SNPs ...
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bioRxiv - Bioengineering 2021Quote: ... Luminescence was measured with a FluoStar Omega plate reader (BMG Labtechnologies) and treated with the protocol and reagents of the Dual-Luciferase Reporter Assay System (Promega). All reagents were used accordingly to the manufactures protocol.
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bioRxiv - Bioengineering 2020Quote: ... Luminescence from the activity of luciferase was measured 48 h post-infection using a TECAN infinite M200 pro plate reader after applying Bright-Glo reagent (Promega) on cells.
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bioRxiv - Biochemistry 2021Quote: ... and firefly luciferase expression was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). Percentage of infectivity was calculated as the ratio of luciferase readout in the presence of mAbs normalized to luciferase readout in the absence of mAb ...