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Citations for Promega :
701 - 750 of 4110 citations for 2'3' cyclic GAMP cGAMP ELISA Kit 384 well Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Luciferase luminescence was read on a FLUOstar Omega plate reader (BMF Labtech) using the Luciferase Assay System (Promega).
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bioRxiv - Microbiology 2021Quote: ... Plates were incubated for 24 h and viability was measured following a 6 h incubation with CellTiterBlue (Promega). Viability was quantified by fluorescent measurement (Ex ...
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bioRxiv - Molecular Biology 2022Quote: ... 100uL of cells were transferred to an opaque white bottomed plate and 100uL of Cell-Titer Reagent (Promega) was added ...
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bioRxiv - Genomics 2023Quote: ... We determined luciferase luminescence on a FLUOstar Omega plate reader (BMF Labtech) using the Luciferase Assay System (Promega). For all pseudovirus experiments ...
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bioRxiv - Cell Biology 2023Quote: ... the plate was assayed using the Dual-Glo Luciferase Assay System according to the manufacturer’s instructions (Promega, E2920) and read using a Microplate Luminometer (BioTek ...
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bioRxiv - Plant Biology 2024Quote: ... and incubated for 1 h at room temperature before measuring luminescence using a GloMax Explorer plate reader (Promega). A standard curve was used for quantification of the UDP produced ...
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bioRxiv - Bioengineering 2024Quote: ... The plates were brought to room temperature and 100 μL of room temperature CellTiter-Glo 3D (Promega G9683) were added to each of the wells ...
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bioRxiv - Genomics 2023Quote: ... DNA was extracted using a commercial kit (Wizard Genomic DNA Purification Kit, Promega, Madison, WI). Libraries were produced by first fragmenting 75 ng DNA with the Nextera reagent (Illumina ...
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bioRxiv - Zoology 2024Quote: DNA was extracted with several different kits including the Wizard Genomic DNA Purification kit (Promega) and the Qiagen Blood & Tissue kit ...
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bioRxiv - Cancer Biology 2021Quote: ... 40 000 cells were seeded/well and ATP levels were measured by CellTiter-Glo Luminescent (CTG) Cell Viability Assay (Promega) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... All cells in each well were lysed and luciferase was measured using ONE-Glo™ Luciferase Assay reagent (Promega, US). RLUs are per well of a 96-well plate.
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bioRxiv - Immunology 2022Quote: ... injecting 50 μl per well of coelenterazine substrate (Nanolight Technologies, 2 μg/ml) and analysing luminescence on a FLUOstar OPTIMA luminometer (Promega). Data were normalised to a mock-treated control to generate a fold induction ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μl of lysate was transferred to a black 96-well Optiplate using 50 μl of Firefly and 50 μl of Renilla detection reagents (reagents were either from Promega or home-made ...
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bioRxiv - Microbiology 2019Quote: Protein digestion - for samples processed with reagent 1 and 2 as well as for supernatants (proteomes) MS grade trypsin (Promega) was used at 1:1000 w/w protease:protein ...
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bioRxiv - Microbiology 2020Quote: ... injecting 50 μl per well of coelenterazine substrate (Nanolight Technologies, 2 μg/ml) and analysing luminescence on a FLUOstar OPTIMA luminometer (Promega). Data were normalized to a mock-treated control to generate a fold induction.
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bioRxiv - Microbiology 2021Quote: ... the media was discarded and the wells were washed with PBS before adding 50 μl of Passive lysis buffer (Promega). Cells were frozen at −20°C to facilitate complete lysis.
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bioRxiv - Microbiology 2020Quote: ... was added to each well and incubated for 2 min or cells were lysed with Cell lysis buffer (Promega E1500) and placed on a rocker for 15 min ...
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bioRxiv - Microbiology 2019Quote: ... injecting 50 µl per well of coelenterazine substrate (Nanolight Technologies, 2 µg/ml) and analysing luminescence on a FLUOstar OPTIMA luminometer (Promega). Data were normalised to a mock-treated control to generate a fold induction.
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bioRxiv - Biophysics 2020Quote: ... 70 μL of PBS were added to each well followed by addition of 10 μL of a 50 μM coelenterazine-h solution (Promega). After 10-min incubation ...
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bioRxiv - Immunology 2022Quote: ... were transfected in keratinocytes (24 wells, cultured with 400 µl KGM2) using the transfection reagent Fugene HD (Promega, Madison, WI). 24 h after transfection ...
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bioRxiv - Microbiology 2022Quote: ... was used to measure the luciferase activity of each well after the addition of 50-100 µl of reconstituted luciferase assay buffer (Promega) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... was used to measure the luciferase activity of each well after the addition of 50-100 µl of reconstituted luciferase assay buffer (Promega). Inhibitors were normalized to vehicle control.
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bioRxiv - Microbiology 2021Quote: ... Cells were then transfected with plasmids DNA (250 ng per plasmid per well) using FuGENE HD (Promega, 1:8 ratio), and cultured at 28°C ...
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bioRxiv - Cancer Biology 2020Quote: ... cell numbers in both upper and lower wells were measured via either conventional cell counting or using Cell Titer Glo (Promega) as described previously (Alkan et al. ...
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bioRxiv - Biophysics 2021Quote: ... The cell suspension was dispensed in a 96-well white bottom plate at a volume of 80 μL per well and loaded with 20 μL of 25 μM Nano-Glo Vivazine Live Cell Substrates (Promega) diluted in the assay buffer ...
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Herpes simplex virus 1 entry glycoproteins form stable complexes prior to and during membrane fusionbioRxiv - Microbiology 2022Quote: ... the media of the effector cells was replaced with 40 µl per well of fusion medium with 1:500 Enduren luciferase substrate (Promega) added ...
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bioRxiv - Microbiology 2019Quote: ... An equal amount of BacTiter-Glo reagent was added to each well as described by the manufacturer (BacTiter-Glo Microbial Cell Viability, Promega). For ATP quantification under normoxia ...
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bioRxiv - Molecular Biology 2019Quote: ... Relative numbers of viable cells in each well were determined using CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega). Experiments were performed in six biological replicates ...
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bioRxiv - Cancer Biology 2021Quote: ... media in each well was replaced with 70 μl fresh medium 70 μl of Cell Titer-Glo reagent (Promega #G7570) was added ...
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bioRxiv - Cell Biology 2021Quote: ... The numbers of cells that migrated into the lower chamber as well as those detached by Trypsin-EDTA were quantified by CellTiter-Glo (CTG) assay (Promega). % Migration = the number of cells that migrated in response to chemoattractant / the total number of cells seeded on the chamber.
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bioRxiv - Microbiology 2020Quote: ... by counting the number of green cells in each well using an Acumen eX3 scanner or by measuring luciferase activity with Steady-Glo Luciferase assay system (Promega) using EnVison (Perkin Elmer).
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bioRxiv - Microbiology 2021Quote: PS cells were seeded at 1.5 × 105 cells per well in 96-well plates (n=3) and transfected with in vitro transcribed TBEV replicon RNA (100 ng/well) and 10 ng of pTK-Ren (Promega) using Lipofectamine-2000 (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... culture supernatant was removed from each well and replaced with 0.3 ml of ONE-Glo luciferase reagent (Promega, Madison, WI). The plates were shaken at 400 rpm for 10 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were then transiently-transfected with 100 ng/well cDNA encoding NLuc/NRP1 or NLuc/VEGFR2 using FuGENE (Promega, USA) according to the manufacturer’s instructions and incubated for a further 24h at 37°C/5% CO2 ...
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bioRxiv - Genetics 2020Quote: ... 2 µg NFIA expression construct (NFIA-FLAG) was transfected to each well of cells by FuGENE HD Transfection Reagent (Promega). Nuclear extracts containing the NFIA protein were extracted from 3 wells of transfected cells using nuclear and cytoplasmic extraction kit (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... 40 µL of medium from selected wells was replaced with Nano-Glo HiBiT extracellular buffer with LgBiT and furimazine substrate (Promega). Readings were taken after a 30 min room temperature incubation as described above.
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bioRxiv - Molecular Biology 2021Quote: A total of 10 μg of bacmid DNA was transfected into 6 wells of 2×106 adherent Sf9 cells (at 5×105 cells/ml) using the transfection reagent Fugene HD (Promega). 42–72 h post transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... The wells were co-transfected with different PFKFB3-HRE luciferase constructs along with pRL-TK renilla luciferase plasmid (Promega, E2231). 12hrs post-transfection ...
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bioRxiv - Microbiology 2022Quote: ... media in each well was replaced with 70 μl of fresh media and 70 μl CellTitre-Glo reagent (Promega #G7570). For suspension cells ...
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bioRxiv - Molecular Biology 2023Quote: ... For ACE2 overexpression HEK293T cells were transfected for 24 h with human ACE2 (pCG1-hACE2, 200 ng DNA/well) using JetPrime (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... We estimated the number of infectious virus after two days by quantifying the luminescence in each well using Bright Glo Luciferase Assay System (Promega). Differences in relative luciferase units (RLUs ...
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bioRxiv - Immunology 2023Quote: The same 96 well setup was used as for the PBMC assay with 50000 T2 and 50000 Jurkat NFAT-Luciferase cells per well (Promega) in 100ul R10 medium ...
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Engineering synthetic CopT/A-based genetic circuits for miRNA imaging and functional gene regulationbioRxiv - Synthetic Biology 2023Quote: ... 50 μL of coelenterazine substrate was added to the wells and Gluc activity was measured using a Glomax-20/20 luminometer (Promega).
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bioRxiv - Immunology 2023Quote: ... pI.18-3xFlag-myRIG-I or pI.18-3xFlag-roRIG-I (25 ng/well each) together with transfection control pLR-SV40-Renilla (Promega) (50 ng/well ...
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bioRxiv - Molecular Biology 2023Quote: ... The media mixture was removed from each well and the cells were lysed with a room tempered 1xGlo buffer (Promega) for 5 min at 25°C while shaking at 550 rpm ...
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bioRxiv - Microbiology 2023Quote: ... For transient expression experiments each well was transfected with 100 ng of DNA using 1:3 ratio of FUGENE HD (#E2311, Promega). Twenty-four hours after transfection or doxycycline stimulation ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed after exactly 24 hours of stimulation in 1X Passive Lysis Buffer (100 μl per well (Promega, #E1941)) ...
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bioRxiv - Cancer Biology 2023Quote: ... where 20 μl of yeast culture was mixed in each well with an equal volume of 2x Passive Lysis Buffer (Promega) to permeabilize cells by 15 minutes of shaking at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... 1 × 106 Sf9 cells in 2 mL of media in a 6-well dish were transfected with up to 2 µg of fresh bacmid DNA using FuGene HD transfection reagent (Promega) at a ratio of 3:1 (Fugene reagent:DNA ...
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bioRxiv - Microbiology 2023Quote: ... injecting 50 μl per well of coelenterazine substrate (Nanolight Technologies, 2 μg/ml) and analysing luminescence on a FLUOstar OPTIMA luminometer (Promega). Fold inductions were calculated by normalising to a mock-treated control.