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Citations for Promega :
7601 - 7650 of 8253 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: The Ythdf2 promoter region ranging from −2000 bp to +100 bp of the TSS was amplified from murine NK cells and cloned into a pGL4-basic luciferase reporter vector (Promega) to generate a pGL4-Ythdf2 reporter plasmid ...
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bioRxiv - Immunology 2021Quote: ... Cells were then harvested and washed prior to cell lysis and addition of luciferin substrate from the Bright-Glo Luciferase Assay System (Promega). The resulting luminescent signal was measured using a Tecan Infinite M200 Pro ...
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bioRxiv - Biochemistry 2020Quote: ... Bacmid DNA was prepared by isopropanol precipitation and transfected into Sf9 (Spodoptera frugiperda) insect cells using FuGENE HD transfection reagent (Promega). After two rounds of virus amplification in Sf9 cells ...
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bioRxiv - Microbiology 2021Quote: Replication-deficient SARS-CoV-2 Spike pseudotyped lentivirus particles were generated by co-transfecting GFP-luciferase vector and ancestral4 or Beta18 spike expression constructs with lentivirus packaging and helper plasmids into 293T cells using Fugene HD (Promega) as previously described 22 ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were then washed three times with 1 × PBS and blocked with 3% Blot-Qualified bovine serum albumin (BSA, Promega) in 1 × PBS containing 0.05% Tween-20 (TPBS ...
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bioRxiv - Microbiology 2020Quote: ... Cytopathic effect (CPE) was indirectly quantified as the presence of ATP in live cells by using the CellTiter-Glo (Promega) luminescent cell viability assay at 72 hours post-infection ...
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bioRxiv - Microbiology 2021Quote: ... 2 μg of each env clone was co-transfected with 4 μg of pNL4.3-ΔEnv-vpr+-luc+ into 293T cells in a T25 flask using the FuGENE6 transfection reagent (Promega, USA). The culture supernatants containing the pseudoviruses were harvested at 72 hours post transfection ...
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bioRxiv - Cancer Biology 2021Quote: Apoptosis and cell viability studies were performed using the Caspase-Glo® and CellTiter-Glo® luminescence-based assays (Promega) as described previously 19 ...
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bioRxiv - Bioengineering 2020Quote: The metabolic activity of MSCs and ECs was evaluated by CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega) individually ...
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bioRxiv - Bioengineering 2020Quote: ... The Fluc and Rluc luminescence of the cells was quantified using the Dual-Luciferase® Reporter Assay System (Promega, E1910) following the manufacturer’s instructions.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were lysed 24 h after chemical exposure to measure luciferase activity (Firefly luciferase assay system; Promega Madison WI, USA) and β-galactosidase activity ...
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bioRxiv - Neuroscience 2020Quote: ... ZF4 cells were seeded on glass coverslips and transfected with plasmids encoding Pcdh19-GFP and Ryk-HA using Fugene HD (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant viral DNA was extracted under alkaline conditions, followed by ethanol precipitation (Birnboim and Doly, 1979) and used for transfection into insect cells (Fugene HD, Promega). Baculovirus recombinant for COL6A3WT or COL6A3ΔC4 were propagated in Sf21 cells and supernatant (SN ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 and 6 d using a CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) (G3582; Promega, Madison, WI). Cells were incubated with the MTS reagent 3-4 h ...
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bioRxiv - Cancer Biology 2020Quote: ... Relative cell number was determined by a fluorescence assay based on the measurement of a constitutive protease activity restricted to intact viable cells and that is independent of the cellular metabolic activity (CellTiter-Fluor, Promega). The fluorescence signal was kept within the linear range and was proportional to cell number ...
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bioRxiv - Cell Biology 2021Quote: Transient transfection of A36-p14 hybrid constructs and the pE/L-LifeAct-iRFP670 expression in Vaccinia-infected cells was done using FUGENE (Promega) as described by the manufacturer ...
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bioRxiv - Cancer Biology 2020Quote: ... 20 μL of cell lysate was transferred to 96-well plates and placed in a 96-well microplate luminometer (GloMax-Multi, Promega). 100 μL Luciferase Assay Reagent II and 100 μL Stop & Glo Reagent (both provided in the Dual-Luciferase Reporter kit ...
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bioRxiv - Cancer Biology 2020Quote: ... The cell lysates were transferred to a 96-well black plate and then an equal volume of Luciferase assay reagent (Promega) was added ...
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bioRxiv - Cancer Biology 2020Quote: ... 72 and 96 hours with a Tecan Infinite M200PRO reader using the CellTiter-Glo® Luminescent Cell Viability Assay according to manufacturer’s directions (Promega). For WCM154 cells ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell line identities were regularly confirmed by short tandem repeat (STR) profiling using the PowerPlex16 system and GeneMapper software (Promega). Cell lines were regularly screened for mycoplasma.
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bioRxiv - Cell Biology 2021Quote: ... the luciferase activities were measured in 20 μl of cell-lysate using the dual-luciferase assay system (Promega, Madison, WI) with a luminometer (LumiCount ...
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bioRxiv - Cell Biology 2021Quote: ... Cell death was measured 24 hours post seeding via luminescence using the Caspase Glo assay (Promega, Madison, WI, USA: #G8090). Annexin-V /7AAD staining (Biolegend ...
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bioRxiv - Microbiology 2020Quote: Replication competent HIV-1 and VSV-G pseudotyped HIV-1 GFP vectors were produced by transfection of HEK293T cells in T150 flasks using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... before treatment without or with OSM (10 ng/ml, 45 min) and RNA extraction (ReliaPrep RNA Cell Miniprep System, Promega). cDNA was generated using oligo(dT)20 primer (GoScript Reverse Transcription System ...
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bioRxiv - Systems Biology 2021Quote: ... Collisional energy was ramped stepwise as a function of ion mobility.24 Peptide loading was normalized to the total ion chromatograms from 200 ng injections of K562 whole cell lysate standard (Promega).
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bioRxiv - Neuroscience 2021Quote: ... Cells were harvested 24 hours post-transfection and RLuc and FLuc activities were measured by Dual Glo luciferase assay (Promega), according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2021Quote: ... or chimeric infectious clone plasmid was transfected into C6/36 or 293T cells by FuGENE 6 transfection reagent (Promega, USA). At 72 h post-transfection ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were transfected using a 1:3 ratio of human μOR and a splitluciferase based cAMP biosensor (pGloSensorTM-22F; Promega). Transit 2020 (Mirus Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... The cells were transferred to white- walled 96-well plates and processed using the Bright-Glo Luciferase Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR was performed with 40 ng of cDNA for the cell and 1.6 ng of cDNA as the template and GoTaq™ master mix according to manufacturer instructions (Promega). The oligonucleotides were designed with PRIMER-Blast using the following parameters ...
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bioRxiv - Microbiology 2020Quote: ... MTS assays were performed to measure the cell viability using CellTiter 96 AQueous One reagent (G3580, Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... cytotoxicity of Bordetella infection was measured as lactate dehydrogenase (LDH) release into cell culture media using CytoTox 96 assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the transduction efficiency was quantified by measuring the firefly luciferase activity in cell lysates using the firefly luciferase substrate of the dual-luciferase reporter assay system (Promega) and a Glowmax plate luminometer (Promega).
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bioRxiv - Molecular Biology 2021Quote: ... Luciferase assays were performed on 20 μl of lysate or 1x Cell Culture Lysis Reagent in 96 well plates on the GloMax-Multi Detection System (TM297, Promega). 100 μl Luciferase Assay Reagent was added to wells ...
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bioRxiv - Microbiology 2020Quote: ... Infected SupT1 cells were incubated for 48 h and lysed in 100 μL of Bright-Glo Luciferase Reagent (Promega, E2610). Infection was assessed by luciferase activity using a LUMIstar Omega microplate luminometer (BMG Labtech) ...
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bioRxiv - Microbiology 2020Quote: ... 2×105 HeLa-HA cells were seeded per well of a six-well plate and transfected using Fugene-HD transfection reagent (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... and luciferase activity was measured in 3 µl aliquots of the cell lysates using a Dual-Luciferase Reporter Assay System (Promega). Firefly luciferase activity was normalized with that of Renilla luciferase.
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bioRxiv - Microbiology 2020Quote: ... at 9 h post-electroporation and luciferase activity was measured in 10 µl aliquots of the cell lysates using a Luciferase Reporter Assay System (Promega).
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bioRxiv - Cancer Biology 2020Quote: ... and cells were lysed in a mixture of 50 µL media and 50 µL CellTiter-Glo 2.0 reagent (Promega # G9243) per well ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293T cells were seeded in 24-well tissue culture plates at a density of 1.6 x 105 cells/well and transfected with 50-100 ng/well of pNL4-3-GFP using Fugene HD (Promega) transfection reagent ...
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bioRxiv - Biophysics 2021Quote: ... sapiens TACAN-eGFP or the M2 muscarinic receptor (Halo-M2R-eGFP) plasmid (previously established in this lab) was transfected into the cells using FugeneHD (Promega) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2020Quote: ... We stained the cells with 3-6 pM Halo-SiR ligand 48 directly before imaging according to the protocol of the manufacturer (Promega) to obtain equal molecular densities during imaging and therefore minimize differences in tracking losses.
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bioRxiv - Biophysics 2020Quote: ... as well as expression construct of interest in a 9:8:1 mass ratio into HEK293T cells using FuGENE HD Transfection Reagent (Promega) per manufacturer’s protocol ...
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bioRxiv - Biophysics 2020Quote: ... mass ratio) into LentiX cells grown to approximately 80% confluency in 6-well plates using FuGENE HD Transfection Reagent (Promega) per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were cultured for 48 h and the luciferase activity was measured using the Dual-Luciferase Reporter Assay System (Promega). The final firefly luciferase activity was normalized to the co-expressed renilla luciferase activity.
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bioRxiv - Cell Biology 2021Quote: ... Cells were treated with the indicated concentration of CHIR99021 24 hours after transfection and after another 24 hours medium was removed and the cells were harvested with 50 µl Passive Lysis Buffer (E1941, Promega). Luciferase activity was measured on a GloMax Navigator (Promega ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were lysed for 15 minutes on ice then treated with 10 µL of RQ1 DNase (Promega, catalog no. M610A) for 5 minutes at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... RPE1 wild type cells were transfected with PX459 plasmid containing the corresponding guide sequence for 24 hours using FuGENE 6 (Promega). Following 72 hours of 4 μM Puromycin selection ...
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bioRxiv - Genomics 2022Quote: DNA was isolated from blood cells using a modified column approach based on the SV total RNA isolation system (Promega). Frozen samples were thawed on ice ...
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bioRxiv - Molecular Biology 2022Quote: ... were co-transfected with psPAX2 and pCMV-VSV-G packaging plasmids into HEK293T cells using FuGENE 6 (Promega, WI, USA). Supernatants containing virus were collected at 48h and 72h after transfection ...