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Citations for Promega :
7551 - 7600 of 8253 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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A detailed kinetic model of Eastern equine encephalitis virus replication in a susceptible host cellbioRxiv - Systems Biology 2024Quote: NanoLuciferase protein expression was measured from triplicate collections of EEEV FL93 nLuc infected cell lysates by the manufacturer’s protocol (Promega) as previously described in [152] on a Biotek Synergy LX plate reader.
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bioRxiv - Genomics 2024Quote: The lentivirus was generated according to standard methods by the transfection of 293T/17 cells with Fugene HD (Promega), our library vector ...
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bioRxiv - Biophysics 2024Quote: ... Dishes of cells were then stimulated with light and luminescence values were recorded using GloMax 20/20n Luminometer (Promega). A green (495 nm ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were co-transfected with 2 μg SBE4-luc reporter and 0.2 μg renilla luciferase vector (PRL-TK, Promega) using the X-tremeGENE 360 transfection reagent (Sigma ...
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bioRxiv - Biophysics 2024Quote: Tracking was performed in Human Bronchial Epithelial Cells (HBEC) labeled with between 0.5 pM – 1 pM JF646 dye (Promega). At this concentration of dye ...
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bioRxiv - Cell Biology 2024Quote: pSBtet-BP plasmids encoding Arf6-eGFP or Arf6-UltraID were co-transfected with pCMV(CAT)T7-SB100 into ARPE-19 cells using FuGENE HD transfection reagent as per the manufacturer’s protocol (Promega). Selection of stably engineered cells was performed in growth media supplemented with 2 μg/mL puromycin for 2-3 weeks ...
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bioRxiv - Cell Biology 2024Quote: ... The lentiviral Titer Unit (TU) was extrapolated by measuring the fraction of viable cells with CellTiter-Glo 2.0 and GloMax Plate reader (Promega). For plasmids bearing a fluorescent probe ...
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bioRxiv - Bioengineering 2024Quote: Cytotoxicity was assessed via the CellTiter 96® Aqueous One Solution Cell Proliferation Assay (Promega Corporation, Madison, WI, USA). Cells were seeded in 96 well plates (Techno Plastic Products AG ...
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bioRxiv - Cell Biology 2024Quote: ... the cells expressing H3.2-HaloTag or CDC45-HaloTag were fluorescently labeled with 50 nM HaloTag TMR ligand (G8252; Promega) or JF646 ligand (GA1120 ...
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bioRxiv - Biochemistry 2024Quote: ... ATP bioluminescence was assessed at 13 days after gene knockout with the CellTiter-Glo Luminescent Cell Viability Assay (Promega). For crystal violet staining assays ...
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bioRxiv - Developmental Biology 2024Quote: Plasmids described above or in our previous work(13) were transfected into Neuro2a cells (ATCC) with Fugene 6 (Promega). The transfected cells were selected by puromycin and subjected to RNA extraction with Trizol (Sigma) ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells at 60%-70% confluency were transfected with a total of 50 ng of plasmidic DNA using Fugene (Promega) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Purified bacmids carrying the gene of interest were used to transfect Spodoptera frugiperda derived Sf9 cells with FuGENE following the supplier’s instructions (Promega). At day 4 post-transfection ...
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bioRxiv - Cell Biology 2024Quote: ... Total cytotoxicity of the compounds was then measured using the CellTiter-Glo® Luminescent Cell Viability Assay (G7571, Promega), as previously described ...
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bioRxiv - Molecular Biology 2021Quote: The effect of menadione on the induction of oxidative stress was determined by measuring the ratio between oxidized and reduced glutathione (GSH/GSSG) using a commercial kit by Promega (GSH/GSSG-Glo Assay, V6611).
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bioRxiv - Immunology 2020Quote: ... For the luciferase assay we mixed 20 μl of those supernatants with 100 μl of the luciferase assay reagent (Promega, Luciferase Assay System Kit #E1500) in a well of a white ...
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bioRxiv - Microbiology 2020Quote: Viral RNA was extracted from the OP and CL swab samples collected from chickens and tufted ducks using Maxwell® 16 Viral Total Nucleic Acid Purification Kit on a Maxwell® 16 System extraction robot (Promega, Madison, WI, USA). RNA was isolated from lung ...
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bioRxiv - Physiology 2023Quote: ... 200 ng of total RNA from each sample was reverse transcribed into cDNA using random hexamer primers and GoScript™ Reverse Transcriptase kit (Promega Corporation, Madison, WI, USA). The synthesized cDNA was stored at -20 ℃.
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bioRxiv - Microbiology 2023Quote: ... DNA extraction of fecal cultures was performed using the Maxwell® RSC instrument and the Maxwell® RSC PureFood GMO and Authentication Kit (Promega AG, Dübendorf, Switzerland). Library preparation ...
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bioRxiv - Genomics 2024Quote: ... TFs of interest were expressed as GFP fusion proteins using the TNT SP6 High-Yield Wheat Germ Protein Expression System from Promega (referred to as IVT-kit) following the manufacturer’s instructions ...
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bioRxiv - Zoology 2023Quote: ... Skeletal architecture was examined by hand cutting tissue sections and digesting them with a mixture of 97% Nuclei Lysis Solution (Promega; from the Wizard DNA isolation kit) and 3% 20 mg/ml Proteinase K (Promega) ...
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bioRxiv - Zoology 2022Quote: ... Skeletal architecture was investigated by hand cutting tissue sections and digesting them with a mixture of 97% Nuclei Lysis Solution (Promega; from the Wizard DNA isolation kit) and 3% 20mg/ml Proteinase K (Promega) ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK293TN cells were seeded in 6-well plates and transiently transfected with plasmid of choice using FuGene transfection reagent (Promega) and OptiMem (Gibco) ...
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bioRxiv - Cancer Biology 2021Quote: ... viruses were generated in HEK293T cells transfected with lentiviral packaging vectors along with vectors expressing pGIPZ-shSTEAP3 using Fugene6 (Promega). Two distinct hairpins were chosen for the experiments ...
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bioRxiv - Cancer Biology 2021Quote: ... The 96-well plate was then allowed to equilibrate at room temperature for 30 minutes followed by addition of Cell Titer Glo (Promega) to each well according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were incubated in a cell culture incubator with 5% carbon dioxide at 37 degrees Celcius for 72 hours before cells were washed twice with PBS then measured via CellTiter-Glo (Promega). IC50 experiments were repeated three times for each cell line shown.
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bioRxiv - Cell Biology 2020Quote: ... Homogenates were diluted 1/100 with the extraction buffer and mixed with the luminescent solution (CellTiter-Glo Luminescent Cell Viability Assay, Promega). Luminescence was measured with a SpectraMax Gemini XPS luminometer (Molecular Devices) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were lysed in the lysis buffer that was included in the Dual-Luciferase® Reporter Assay System (Promega, E1910). To the cell lysate ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were then cleared by centrifugation and the supernatant was diluted (1/50) with extraction buffer and mixed with luminescent solution (CellTiter-Glo Luminescent Cell Viability Assay, Promega). The luminescence was measured on a Victor 3 plate reader (Perkin Elmer) ...
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bioRxiv - Cell Biology 2020Quote: ... HCT-OsTIR1 cells were co-transfected with these two constructs together with the PAF1-sgRNA expressing Cas9 plasmid using Fugene HD (Promega), and selected with 100 μg/ml Hygromycin B (Invivogen ...
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bioRxiv - Immunology 2021Quote: Cells were washed twice in D-PBS (Wisent) and lysates were collected using the Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... The plasmids containing indicated CDS and tubP-GAL4 were co-transfected into the cells using FuGENE HD transfection reagent (E2311, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... All cells in each well were lysed and luciferase was measured using ONE-Glo™ Luciferase Assay reagent (Promega, US). RLUs are per well of a 96-well plate.
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bioRxiv - Biophysics 2020Quote: Plasmid DNAs containing Tol2 vectors comprising GV genes and fluorescent protein genes described above and transposase mRNA were introduced to KPL-4 cells by lipofection using FuGene HD (Promega) in OptiMEM (Gibco) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cytotoxicity on mock infected plates was determined using the Promega CellTiter-Glow Luminescent Cell Viability Assay (Promega, Madison, Wisconsin, USA). Half-maximal inhibitory concentration (IC50 ...
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bioRxiv - Molecular Biology 2021Quote: ... and luciferase activity in 10 μl aliquots of the cell lysates was measured using the Dual Luciferase Reporter Assay System (Promega) according to the manufacturer’s protocols ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Reporter gene expression was assessed in 20 µl of cell lysates using the Dual Luciferase Assay and a GloMax luminometer (Promega). To control for transfection efficiency ...
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bioRxiv - Biochemistry 2022Quote: HeLa and HEK293T cells were seeded on 18 mm glass coverslips at a density of 3×105 cells/mL and were transfected with FuGENE 6 (Promega) transfection reagent 24 h later ...
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bioRxiv - Cancer Biology 2021Quote: ... 500 ng of lentiviral plasmid encoding doxycycline inducible transporters and 500 ng of each of the packaging plasmids were transfected into HEK 293T cells with FuGENE 6 transfection reagent (Promega). Cell supernatant was replaced 24 h after transfection and harvested after another 24 h ...
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bioRxiv - Molecular Biology 2020Quote: ... the cells were collected and the luciferase activity was measured using the Dual-Glo Luciferase Assay system (Promega Madisson, USA) and normalized to Renilla Luciferase activity.
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bioRxiv - Molecular Biology 2021Quote: DRD1 Gs-mediated Gs-cAMP accumulation assays with HEK293T (ATCC CRL-11268) were performed using cells transiently expressing human DRD1 and the cAMP biosensor GloSensor-22F (Promega). Cells were seeded (20,000 cells/35 μL/well ...
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bioRxiv - Molecular Biology 2021Quote: Cytotoxicity of BTZ in CCL-210 cells was assessed using a luciferase-coupled ATP quantitation assay (CellTiter-Glo from Promega). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was isolated from cells at the exponential phase (OD600 ~1) with the acidic hot phenol method 41 and treated with DNase (Promega). 50 μg of total cellular RNA was incubated with 1 μM of the corresponding DNAzyme ((Supplementary Table S2 ...
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bioRxiv - Molecular Biology 2021Quote: ... the genomic DNA from all KO cell lines was extracted and the region flanking the Cas9 target site from each ΔPUS cell line PCR amplified and cloned into the EcoRI and HindIII sites of pGEM-3zf vector (Promega). The primers used to amplify the region flanking the Cas9 target sites were the following ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were lysed 24 h after transfection and luciferase activities were measured with the Dual-Luciferase Reporter Assay System (Promega) in a GloMax 20/20 luminometer (Promega) ...
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bioRxiv - Molecular Biology 2021Quote: ... Then the plasmid constructs (150 ng) were transfected into HEK 293T cells at their 90% confluency using Dual-Luciferase Reporter Assay System (Promega). The Luciferase activity was measured 72 h (48h for DsiRNA and 24 h for plasmid constructs ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were then lysed with RIPA lysis buffer containing Protease Inhibition Cocktail and RNase Inhibitor followed by DNase treatment (Promega). The immunoglobin-coated protein magnetic beads (Thermofisher scientific ...
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bioRxiv - Bioengineering 2020Quote: The therapeutic effect of free taxane (pro)drugs and LNP formulations was determined by the [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS)-based CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega) or the resazurin-based PrestoBlue assay (ThermoFisher) ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei were isolated by resuspending cell pellets in 200 μL Dig-wash buffer (CUT&Tag wash buffer supplemented with 0.05% digitonin (Promega, G9441) and 0.05% IGEPAL CA-630 (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... 500 ng of the lentiviral backbone plasmid containing the guide sequence and 500 ng of each of the packaging plasmids were transfected into HEK 293T cells using FuGENE 6 transfection reagent (Promega). The viral media was exchanged after 24 hours ...