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Citations for Promega :
701 - 750 of 1302 citations for Anti PI 4 kinase II beta Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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HIVtat Alters Epithelial Differentiation State and Increases HPV16 Infectivity in Oral KeratinocytesbioRxiv - Microbiology 2023Quote: ... 200 ml of media was treated with 4 U of RQ1 RNase-free DNase (Promega) for 1 hour at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA (4 μg) was reverse-transcribed using oligo-dT and the Reverse Transcription System (Promega). cDNA samples were diluted 1:10 and 5 μl of each sample were used for PCR reactions (final volume of 20 μl ...
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bioRxiv - Biochemistry 2023Quote: ... The pellet was resuspended in 4 M urea containing 0.1 % Protease Max (Promega Corp. V2071) and diluted in 40 mM ammonium bicarbonate buffer ...
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bioRxiv - Plant Biology 2024Quote: ... 4 hours incubation at 37 °C) and 5 μg of trypsin (Trypsin Gold from Promega, in 50 mM TEAB pH 8.5 ...
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bioRxiv - Microbiology 2020Quote: ... and a total of 300 ng treated-RNA was used for reverse transcription reaction with ImProm-II Reverse transcriptase (Promega, Madison, WA, USA). The RT-qPCR was assessed on a Real-time PCR StepOne Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Molecular Biology 2019Quote: ... and the first-strand cDNA was synthesized from 1 μg of total RNA with oligo dT or random primers using ImProm-II reverse transcriptase (Promega, Madison, WI, USA). The qPCR reactions were performed in a 20 μL reaction mixture using SYBR Green PCR Master Mix (Roche ...
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bioRxiv - Physiology 2020Quote: ... The beads were resuspended in 10 μl of reverse transcriptase mix (100□U, SuperScript II, Invitrogen; 10 U, RNAsin, Promega, Madison, Wisconsin, USA), 1□×□Superscript II First-Strand Buffer ...
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bioRxiv - Genomics 2019Quote: ... To this was added: 1 µl of EcoRV-digested pBlueScript KS II+ (∼100 ng) that had been treated with calf intestinal phosphatase (Promega Corp., Madison, WI), and 0.1 µl of T4 DNA ligase (NEB) ...
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bioRxiv - Physiology 2020Quote: ... 500 ng of RNA were reverse-transcribed into cDNA using oligo(dT)16 and ImProm-II TM Reverse Transcriptase (Promega Corp., Madison, USA). cDNA products were amplified on a GeneAmp 7500 Fast System (Applied Biosystems ...
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bioRxiv - Bioengineering 2023Quote: ... Reverse transcription was performed at 70 °C for 5 min and 42 °C for 60 min with 1 µg total RNA using ImProm-II reverse transcriptase (Promega, Madison, WI, USA) and 250 ng oligo(dT)12-18 primers (ThermoFisher ...
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bioRxiv - Cancer Biology 2020Quote: ... the blot was blocked with either 5% nonfat-milk or 5% BSA in TBST before addition of primary antibodies and followed with peroxidase-conjugated secondary antibody (Promega). Protein bands were detected using SuperSignal Chemiluminescent Substrate (Pierce ...
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bioRxiv - Microbiology 2019Quote: The following commercial antibodies and dilutions were used for immunoblotting: rabbit polyclonal antibody to HaloTag (Promega; G9281; 1:1,000 dilution), mouse monoclonal antibody to lamin A/C (Cell Signalling ...
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bioRxiv - Immunology 2020Quote: ... were used as primary antibodies and horseradish peroxidase (HRP)-conjugated secondary antibodies were from Promega (Rabbit W401B and Mouse W402B), and the substrate ECL was detected by Pierce ECL2 solution (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... The panel of ssDNA-labelled antibodies (250 ng/ml for each antibody) was mixed with 2 U/µl RNAsin Plus (Promega) and 0.1% Triton X-100 in PBS:PFBB (1:1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Appropriate HRP conjugate secondary antibodies were from Promega. Signal was detected using the ECL system (BioRAD ...
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bioRxiv - Immunology 2019Quote: ... Secondary antibody (α-Goat HRP, Promega, 1:10,000). The blot was developed using ECL Western Blotting Substrate (Promega ...
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bioRxiv - Cancer Biology 2022Quote: ... Halo-tag (G9211) antibody was purchased from Promega. The C11orf53 antibody was produced in rabbit in house by using full-length C11orf53 recombinant protein as antigen.
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bioRxiv - Immunology 2019Quote: ... NLuc (Lg-BiT) polyclonal antibody (courtesy of Promega), human Caspase-1 p20 Rabbit polyclonal Ab (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2019Quote: ... 2° antibodies α- mouse (HRP detection, Promega, W4021). The mean intensity quantification of 4 independent experiments is shown in Figure 4 – supplement 1B ...
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bioRxiv - Molecular Biology 2022Quote: ... Secondary HRP-conjugated antibodies were purchased from Promega or GE Healthcare (anti-mouse Cat ...
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bioRxiv - Cell Biology 2023Quote: ... an antibody against HaloTag (G9211) was from Promega; an antibody against GFP (7.1/13.1 ...
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bioRxiv - Cell Biology 2022Quote: ... HRP conjugated anti-rabbit (W4011) and anti-mouse (W4021) were from Promega and used at a dilution of 1:10000 ...
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bioRxiv - Cell Biology 2020Quote: ... anti-rabbit (Promega, W401B) antibodies at a concentration of 1:10 000 and anti-rat IgG1 (Monoclonal Antibody Core Facility ...
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bioRxiv - Microbiology 2021Quote: ... anti-Halo-tag (Promega), anti-FLAG (M2 ...
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bioRxiv - Physiology 2022Quote: ... Anti-rabbit (Promega, #W401B), anti-guinea pig (JacksonImmuno ...
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bioRxiv - Cancer Biology 2019Quote: ... rabbit anti-pJNK (Promega) at 1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... anti-Halo (G9211; Promega), anti-Tubulin (100109-MM05T ...
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bioRxiv - Biochemistry 2020Quote: ... anti-rabbit (Promega, #W401B) and anti-chicken (Promega ...
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bioRxiv - Immunology 2021Quote: ... or anti-goat (Promega) secondary antibodies conjugated to horseradish peroxidase ...
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bioRxiv - Genetics 2022Quote: ... anti-rabbit-IgG (Promega), or anti-rat-IgG (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... anti-rabbit HRP (Promega) or anti-mouse HRP (Promega ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... anti-β-galactosidase (Promega, Z3783 ...
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bioRxiv - Plant Biology 2023Quote: ... or anti-mouse (Promega) in 5% low-fat milk in TBS-T for 1 hour ...
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bioRxiv - Physiology 2024Quote: ... anti-rabbit HRP (Promega) or anti-mouse HRP (Promega ...
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bioRxiv - Microbiology 2019Quote: The following commercial antibodies and dilutions were used for immunofluorescence staining: rabbit polyclonal antibody to HaloTag (Promega; G9281; 1:500 dilution), mouse monoclonal antibody to lamin A/C (Cell Signaling ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were examined for viability every 4 to 6 weeks with the CellTiter-Glo assay (Promega). Cell viability was measured in quadruplicates by seeding the cells (2,000 to 3,000 per well in 96-well plate) ...
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bioRxiv - Cell Biology 2021Quote: ... for 4 h at 800 rpm and 42°C or thermolysine (1:50) (Promega, Walldorf, Germany) for 2 h at 800 rpm and 60°C ...
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bioRxiv - Biochemistry 2020Quote: ... and cells were lysed at 4 hours post-transfection with 250 μL passive lysis buffer (Promega) and incubated 20 minutes at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... and cells were lysed at 4 hours post-transfection with 250 μL passive lysis buffer (Promega) and incubated 20 minutes at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 µL of PBS containing 4 μg/mL Hoechst and 1/10000 CellTox Green Dye (Promega) were were dispensed per well 1 h prior to imaging at Cytation5 image cytometer or Opera Phenix (Perkin Elmer ...
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bioRxiv - Cancer Biology 2022Quote: ... and NUMB1-4 (from V.O.R.) expression plasmids was performed using FuGENE HD transfection reagent (Promega, E2311) according to the manufacturer’s protocol and selected with Geneticin (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... Viability was measured every 4 days using the CellTiter-Glo luminescent cell viability assay (Promega G7573). A D300e drug dispenser (Tecan ...
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bioRxiv - Plant Biology 2022Quote: ... and GST- GBF3 were purified from 4 ml culture and immobilized on MagneGST Glutathione particles (Promega). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... then transfected with 4 μg pLZRS-GCaMP6 DNA plus 12 μl FuGENE 6 (Promega Cat. #E2691) in 800 μl of Opti-MEM (Thermo-Fisher Cat ...
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bioRxiv - Microbiology 2023Quote: ... then digested with 4 μL of a trypsin/Lys-C Mix (0.5 μg/uL) (Promega # V5071) using a two-step in-solution digestion overnight at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... or MEK inhibitor U0126 (1, 10, and 50 µM for 4 h; Promega, Madison, WI, USA). A corresponding volume of dimethyl sulfoxide (Nacalai Tesque ...
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bioRxiv - Biochemistry 2023Quote: ... HRP (4 μM) was added followed by 20 μL of Nano-glo luciferase substrate (Promega, N1110) to each well ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 µl of lysate was added to a 1.5 ml Eppendorf tubes containing 100 µl of luciferase assay reagent II (Promega luciferase assay system, Cat# E1500) and the mixture pipetted in and out three times ...
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bioRxiv - Neuroscience 2021Quote: ... The immunoblots were probed with appropriate secondary antibodies (Promega) and visualised by chemiluminescence (Supersignal detection kit ...
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: ... Secondary antibody (donkey α-goat HRP, Promega, 1:10,000). For His-tagged proteins ...