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Citations for Promega :
651 - 700 of 1302 citations for Anti PI 4 kinase II beta Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Firefly luciferase activity was measured by adding 25 μL Luciferase Assay Buffer II (LARII) to 5 μL of lysate and measured immediately with the GloMax 20/20 Luminometer (Promega, E5311). Reactions were stopped and Renilla luciferase activity was measured by adding 25 μL Stop&Go reagent ...
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bioRxiv - Neuroscience 2021Quote: ... First strand cDNA was synthesized in 20 µl reaction using oligo-dt primers and ImProm-II Reverse Transcriptase (Promega, Milan, Italy) with 5 µl of RNA according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... 3 μg of total RNA was incubated with 1mM of Oligo-AP primer (Supplementary Table I) and Improm II reverse transcriptase (Promega®) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: ... was synthesized from 1 μg of total RNA, using oligo-dT primers (USB Corporation, Cleveland, USA) and a reverse transcriptase kit (ImPROm-II, Promega, USA), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was extracted from Hydra polyps starved for 48 hrs and cDNA was synthesized from total RNA using Improm-II reverse transcriptase system (Promega™) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... purified total RNA was reverse transcribed into cDNA by ImProm-II™ Reverse Transcription System (Promega; Madison, WI; Cat. No. A3800). Target gene fragments were first amplified from a mixed cDNA library of planula larva and primary polyps ...
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bioRxiv - Neuroscience 2022Quote: ... First strand of cDNA was synthesized from 0.5-1 μg of total RNA using Im-Prom-II system (Promega, Cat. A3800). Real-Time PCR was performed using iTaq qPCR master mix according to manufacturer’s instructions (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA (1 μg) was reverse-transcribed using oligo(dT)18 and M-MLV reverse transcriptase II (Promega, Fitchburg, WI, USA).
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bioRxiv - Microbiology 2023Quote: ... of virus pool stock was used for viral RNA extraction by GeneAid viral RNA extraction kit (GeneAid) and converted to cDNA using ImProm-II™ reverse transcription system (Promega) according to manufacturers’ protocols ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA (1 μg) was reverse-transcribed using oligo(dT)18 and M-MLV reverse transcriptase II (Promega, Fitchburg, WI, USA).
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bioRxiv - Molecular Biology 2021Quote: ... cells were pulsed for 4 h with CellTiter 96 Aqueous One solution reagent (Promega). Cell viability was measured by a luminometer ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... after which growth media was removed and 20 µl of 4% D-luciferin (Promega) in CO2 independent medium (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: 4 × 105 HEK293T cells were lysed in 100 μl of Passive Lysis Buffer (Promega). 10 μl of cell lysates were separated on a NuPAGE 4-12% Bis-Tris polyacrylamide gel (ThermoFisher) ...
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bioRxiv - Microbiology 2020Quote: ... and then ligated for 16 h at 4 °C with T4 DNA ligase (Promega). After a further purification step ...
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bioRxiv - Biochemistry 2019Quote: 4 x 105 HEK293T cells were lysed in 100 μl Passive Lysis Buffer (Promega). 10 μl of cell lysates were boiled with 2.5 μl 4x gel loading buffer for 5 min at 98°C and run on a NuPAGE 4-12% Bis-Tris polyacrylamide gel for 45 min at 200 V ...
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bioRxiv - Molecular Biology 2020Quote: ... Chromosome 4: 101,716,750-101,718,250 forward strand) was cloned into pGL3-basic vector (E1751, Promega). Reporter vector ...
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bioRxiv - Systems Biology 2023Quote: ... for 4 h at 37 °C and then with trypsin (Promega, 1:100, w:w) overnight at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... synthesized from Gene Universal (USA) at 4°C overnight (T4 DNA ligase, Promega, USA). Cys-loop mutation ligation products were transformed into DH5α (ThermoScientific ...
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bioRxiv - Genetics 2024Quote: ... transfected with 4 µg of plasmid DNA using the FuGENE® HD Transfection (Promega) for 24 h ...
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bioRxiv - Microbiology 2024Quote: ... pH 8.0 before adding 4 µL of 0.5 µg/µL sequencing grade trypsin (Promega) and incubated over night at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... anti-ARC (SantaCruz Biotechnology, #sc-17839, anti-HaloTag (Promega, #G9211), anti-SNAP-tag (New England Biolabs ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by peroxidase-conjugated secondary antibodies (Promega). Signal was developed using ECL substrate (Perkin Elmer) ...
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bioRxiv - Molecular Biology 2022Quote: ... and secondary antibodies (W401B and W402B, Promega). ChIP was performed with anti-TAP and protein A Dynabeads (CAB1001 and 10001D ...
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bioRxiv - Biochemistry 2021Quote: ... and developed using AP-coupled antibodies (Promega) and NBT/BCIP as substrates (Promega).
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibodies used: ßgal (1:100, Promega), pMad (1:800 ...
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bioRxiv - Molecular Biology 2019Quote: ... Two micrograms of total RNA were reverse transcribed into the first strand cDNA using the ImProm-II Reverse Transcription System (A3800, Promega, Madison, USA) according to the manufacturer’s protocol and used as a template for the amplification of the full-length camel IFNε cDNA ...
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bioRxiv - Synthetic Biology 2019Quote: ... The RNA was treated with Promega RQ1 DNase and reverse transcribed into cDNA using the Improm-II Reverse Transcription kit (Promega; Fitchberg, WI). Steady-state transcript levels were then measured using the cDNA template in quantitative PCR (qPCR ...
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bioRxiv - Neuroscience 2021Quote: ... One microgram of total RNA was transcribed to cDNA by reverse transcriptase enzyme action Improm Pre-II ® (Promega, Madison, Wisconsin, USA). Quantitative RT-PCR reaction in real time was done on an ABI Prism ® 7500 Sequence Detection System (Applied Biosystems) ...
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bioRxiv - Cell Biology 2022Quote: ... DNase-treated RNA was transcribed into complementary DNA (cDNA) using the ImProm-II™ reverse transcription system and oligo-dT as primer (Promega, Germany). Quantitative real-time reverse transcriptase polymerase chain reaction (qPCR ...
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bioRxiv - Genomics 2020Quote: Genomic DNA was extracted from whole blood obtained from GALA II study subjects using the Wizard Genomic DNA Purification kits (Promega, Fitchburg, WI), and DNA was quantified by fluorescent assay ...
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bioRxiv - Neuroscience 2023Quote: ... 1ug of total RNA extracted from transfected SK-N-MC cells was reverse transcribed using the ImProm-II Reverse Transcription System and random hexamer primers (Promega, Madison, MI). PowerUp SYBR™ Green Master Mix (Thermo Fisher ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was synthesized with primers specific for each strand separately at 50 °C (Supplemental Table S1) using the ImProm-II™ Reverse Transcription System (Promega, A3800) and subjected to qPCR with primers specific for the viral N-protein (Supplemental Table S1).
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bioRxiv - Cancer Biology 2024Quote: ... cDNA synthesis was performed using 1 µg of total RNA in combination with oligo(dT) and Improm-II reverse transcriptase (Promega, Madison, WI). qPCR analysis was performed as described previously [55] with SYBR green PCR master mix (Applied Biosystems ...
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bioRxiv - Genetics 2024Quote: ... and incubated with primary antibodies in a blocking buffer (1ξ PBS, 0.5% BSA) and then with alkaline phosphatase-conjugated secondary antibody (Promega). The color reaction was developed with NBT/BCIP solution (Nacalai Tesque).
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bioRxiv - Genomics 2019Quote: ... and anti-rabbit-HRP or anti-mouse-HRP 1:10000 (Promega). Blots were visualised with the Fusion Fx7 system and quantified with ImageJ 1.48k.
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bioRxiv - Cell Biology 2020Quote: ... Alkali-phosphatase (AP)-conjugated anti-mouse or anti-rabbit IgG (Promega) as well as AP substrate consisting of 5-bromo-3-chloro-indolyl phosphate (Roche Diagnostics ...
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bioRxiv - Cancer Biology 2021Quote: ... and then resuspended in 4 ml DMEM/F12 + 80 μl DNase (1U/μl) (Promega M6101). The DNase solution was gently shaken by hand for 2–5 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Gel pieces were rehydrated in a mixture of 4 ng/µL trypsin (Promega, Madison, WI) and 0.01% ProteaseMAX (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... followed by a 4 times dilution in Tris buffer and an overnight trypsin digestion (Promega) at a ratio 1/100 ...
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bioRxiv - Cancer Biology 2019Quote: ... The pellet was resuspended in 4 M urea containing 0.1 % Protease Max (Promega Corp. V2071) and diluted in 40 mM ammonium bicarbonate buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... Resuspended grindate was incubated (4°C, 20 min) with 660 U of DNase I (Promega). Insoluble cell debris was pelleted by spinning (16,000 g ...
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bioRxiv - Microbiology 2021Quote: ... 4°C) and then resuspended in 100 µl 1 x Passive Lysis Buffer (PLB, Promega). 45 µl of the luciferase assay substrate dissolved in Luciferase Assay Buffer II was plated on a 96-well plate and 10 µl of the cell lysate was added immediately before measurement of Fluc activity using a Tecan reader ...
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bioRxiv - Systems Biology 2019Quote: ... samples were diluted with 4 volumes of Digestion Buffer (50 mM NH4HCO3) and trypsin (Promega) was added at a ratio of 1:50 ...
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bioRxiv - Systems Biology 2019Quote: ... samples were diluted with 4 volumes of Digestion Buffer (50 mM NH4HCO3) and trypsin (Promega) was added at a ratio of 1:50 ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein samples were digested using 4 μg of trypsin (sequencing grade, low autolysis trypsin; Promega), at 37 °C for 16 h ...
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bioRxiv - Genetics 2022Quote: ... cells were transfected with L1 reporter constructs using 4 μl FuGENE HD transfection reagent (Promega), 96 μl Opti-MEM (Life Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... Compound treatment was initiated 1-4 hours before transfections with FuGENE HD transfection reagent (Promega) following the manual ...
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bioRxiv - Microbiology 2023Quote: ... 200 ml of media was treated with 4 U of RQ1 RNase-free DNase (Promega) for 1 hour at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Fisher) containing 4% FBS with or without HaloTag NanoBRET 618 Ligand (Cat. No. PRN1662, Promega) and incubated overnight at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... The pellet was resuspended in 4 M urea containing 0.1 % Protease Max (Promega Corp. V2071) and diluted in 40 mM ammonium bicarbonate buffer ...