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Citations for Promega :
701 - 750 of 1522 citations for 5 Methylsulfamoylmethyl 1H indole 3 carboxylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Caspase activity was determined using Caspase-Glo® 3/7 Assay Systems (Promega, Madison, WI) according to the manufacturer’s specifications ...
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bioRxiv - Microbiology 2021Quote: ... caspase activity was measured using Caspase-Glo 3/7 Assay according to manufacturer’s instructions (Promega). Staurosporine (1μM ...
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bioRxiv - Biochemistry 2020Quote: FLAG-β2AR HEK 293 cells were transfected with 3 µg cAMP luciferase plasmid DNA (Promega) using Turbofect (ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: ... treated with 3 units/sample of DNase I for 2 hours (Promega, Madison, WI, USA), and 1-3μl aliquots were taken for reverse transcription (RT ...
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bioRxiv - Neuroscience 2020Quote: Caspase activity was measured using a chemiluminescent assay kit (Promega Caspase-Glo® 3/7) in 96-well format using an Analyst HT plate reader (Molecular Devices ...
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bioRxiv - Cancer Biology 2020Quote: ... Plasmid transfections required 3 μg/well and were carried out using 0.1% Fugene HD (Promega).
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bioRxiv - Cell Biology 2021Quote: ... and incubated for 24 hr before adding 40 μl of Caspase-Glo 3/7 (Promega) to each well ...
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bioRxiv - Cancer Biology 2021Quote: ... The remaining cells in the inserts were analyzed with Caspase-Glo 3/7 assay (Promega) to confirm that Jurkat cells incubated with TAM CM did not induce apoptosis compared to the control medium.
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bioRxiv - Neuroscience 2021Quote: ... HA-tagged NPHP1 expression vectors were transfected to mIMCD-3 cells using FuGENE HD (Promega) following the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2020Quote: ... Caspase activation was measured using the CaspaseGlo 3/7 Assay Kit (Promega, Madison, WI, USA) according to manufacturer’s instructions for the indicated time point presented in the figure legend ...
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bioRxiv - Microbiology 2020Quote: ... PacC222 and PacC80 proteins were individually expressed in the pGEX-4T-3 vector (Promega, USA) in E ...
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bioRxiv - Genomics 2021Quote: All the reporter constructs were cloned into pGL-3 promoter plasmids (Promega, Madison, WI, USA). Fragments of the candidate RSCNEs were cloned into pGL3-promoter vector digested by BamH I and Sal □ downstream of the luciferase gene ...
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bioRxiv - Developmental Biology 2023Quote: ... The prospero 3’UTR [2131bp] was subcloned into the pmirGlo Dual-Luciferase expression vector (Promega) downstream of the firefly luciferase gene ...
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bioRxiv - Cancer Biology 2022Quote: ... ATP levels were measured using the CellTiter-Glo 3-D Reagent (Promega, catalog no. G9681) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... 2) chymotrypsin and lysyl endopeptidase (lys-C) (Fujifilm Wako) 3) lys-C and trypsin (Promega) and 4 ...
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bioRxiv - Cell Biology 2022Quote: ... Apoptosis was assessed by commercially available caspase 3/7 assay (Cat # G8090, Promega, Madison, WI) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Apoptosis activities were detected using substrate from the Caspase-3 Glo Apoptosis kit (Promega, WI).
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bioRxiv - Cell Biology 2024Quote: ... washed with ammonium bicarbonate (0.1M) and proteolyzed (0.4µg of LysC (Wako) for 3 hours and 1.2µg of Trypsin (Promega) overnight) ...
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bioRxiv - Microbiology 2024Quote: ... 125 µM of deoxynucleosides triphosphate mix (dNTPs) and 3 u of Pfu DNA polymerase (Promega). Reaction was run with a first step of 4 min of denaturalization at 94°C ...
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bioRxiv - Neuroscience 2024Quote: ... The brain surface was covered in 2-3% low melting point agarose (Promega, Madison, WI) in sterile saline and then capped with silicone elastomer ...
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bioRxiv - Bioengineering 2024Quote: ... A complex of 1 μg of plasmid was mixed with 3 μl of Viafect (Promega) in OPTI-MEM and mixed and incubated at room temperature for 20 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... and apoptosis was measured at different time points using Caspase-Glo 3/7 (Promega, G8091), according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... After 3 days cell viability was assessed using CellTiter-Glo as per manufacturer’s instruction (Promega) on a SpectraMax I3 (Molecular Devices ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was assessed after 3 days of treatment using the CellTiter-Glo assay (Promega) and SpectraMax I3 microplate reader (Molecular Devices).
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bioRxiv - Molecular Biology 2021Quote: ... In vitro translation reactions were carried out with 5 ul of either supplemented rabbit reticulocyte lysate or oocyte extract in total volume of 20 µl containing 10 µM amino acid mix (Promega, L4461), 0.5 units SUPERase•In ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was extracted from lung tissue using the Maxwell automated instrument and the Maxwell® RSC Viral Total Nucleic Acid Purification Kit (Promega). The concentration of RNA and quality were measured with HS Total RNA 15nt.methods (Agilent ...
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bioRxiv - Cell Biology 2021Quote: ... for 4 hours at 37°C and boosted with an extra 2µl Trypsin/LysC (Promega V5073: 20ug + 80uL 50mM acetic acid) overnight at 37°C ...
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bioRxiv - Microbiology 2021Quote: RNA from lysed and heat-inactivated samples was purified according to the instructions of the manufacturer using Maxwell 16 viral total nucleic acid purification kit (Promega, #AS1150) or Maxwell 16 LEV simplyRNA cell kit (Promega ...
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bioRxiv - Microbiology 2023Quote: ... Nucleic Acid Purification with Proteinase K digestion was performed using Maxwell ® RSC Total Viral Nucleic Acid Purification Kit according to the manufacturer’s protocols (Promega, USA). For preparation of bacterial genomic DNA ...
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bioRxiv - Microbiology 2022Quote: Total RNA from each lung homogenate and nasal wash was isolated using the Maxwell RSC Maxwell RSC Viral Total Nucleic Acid Purification Kit (Promega Corporation), following the manufacturer’s suggested protocol ...
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bioRxiv - Microbiology 2023Quote: ... the ladder region was cut off and stained for 30 min in the running buffer containing 10000x diluted Diamond™ Nucleic acid dye (Promega). The transfer of the RNA from the agarose gel to the nylon membrane (Amersham Hyband™-N+ ...
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bioRxiv - Biochemistry 2024Quote: ... cells were lysed with 100 µL of 1X luciferase cell culture lysis reagent (25 mM Tris-phosphate pH 7.8, 2 mM DTT, 2 mM 1,2- diaminocyclohexane-N,N,Ń,Ń-tetraacetic acid, 10% glycerol, 1% Triton X-100, Promega), and luminescence was measured using the Luciferase Assay System (Promega) ...
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bioRxiv - Biochemistry 2024Quote: ... The gels were imaged using ChemiDocTM MP Imaging system using the Rhodamine filter and then counterstained with DiamondTM Nucleic Acid dye (Promega Corporation) to visualize the primer bands ...
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bioRxiv - Immunology 2021Quote: ... vRNA was isolated from plasma samples using the Maxwell Viral Total Nucleic Acid Purification kit on the Maxwell 48RSC instrument (Promega, Madison WI). vRNA was then quantified using a highly sensitive qRT-PCR assay based on the one developed by Cline et al.99 ...
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bioRxiv - Microbiology 2021Quote: ... The reaction mixture was prepared into 96-well plates as follows: 1 μl of 1 mM amino acid mixture minus methionine (Promega, Cat: L9961), 35 μl of rabbit reticulocyte lysate ...
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bioRxiv - Pathology 2022Quote: ... or 300 uL urine+DMSO using the Maxwell RSC Viral Total Nucleic Acid Purification Kit on the Maxwell 48 RSC instrument (Promega, Madison, WI).
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from these bacterial suspensions using a Maxwell® 16 automated nucleic acid purification system and the Maxwell® tissue DNA purification kit following the manufacturer’s instructions (Promega, UK). Purified DNA extracts were treated with RNase (New England BioLabs ...
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bioRxiv - Molecular Biology 2020Quote: ... the reaction mix in a final volume of 83 µl has been prepared by adding 21 µl of the Amino Acid Mixture (for a final concentration of 0.13 mM) and 80 units of RNasin (Promega Ref. number N2511) to 60 µl of RRL ...
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bioRxiv - Evolutionary Biology 2021Quote: Viral RNA (vRNA) was extracted from 100 μl of VTM using the Viral Total Nucleic Acid Purification kit (Promega, Madison, WI, USA) on a Maxwell RSC 48 instrument and eluted in 50 μL of nuclease-free H2O.
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bioRxiv - Microbiology 2023Quote: Total nucleic acid extraction from swab and tissue samples was performed using ReliaPrep™ Viral TNA Miniprep System (Promega, Madison, WI, USA). Swab samples were simply inverted in the tube and briefly centrifuged prior to extraction ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA was extracted using the Maxwell 16 LEV simply RNA tissue kit in combination with a Maxwell nucleic acid extraction robot (Promega, WI, USA) for rabbit caecal content ...
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bioRxiv - Pathology 2024Quote: ... amniotic fluid and eluates from swab samples) using the Viral Total Nucleic Acid Purification Kit for the Maxwell RSC instrument (Promega, Madison WI) with the following modifications ...
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bioRxiv - Cell Biology 2024Quote: ... five µg protein were reduced by 2.5 mM dithiothreitol for 30 min at 37°C and alkylated with 10 mM iodoacetic acid for 30 min at 37°C in the dark before digestion by trypsin (Promega, Walldorf, Germany) in a protein to enzyme ratio 25:1 overnight at 37°C ...
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bioRxiv - Cell Biology 2021Quote: To analyze the 5’ UTR variants we used the pGL3-Basic (Promega), amplification of the 1.4 kb of the 5’ UTR region was carried out using Phusion High Fidelity polymerase (ThermoFisher ...
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bioRxiv - Plant Biology 2021Quote: ... 5 µl of 5X GoTaq Flexi reaction buffer (Promega, Madison, WI, USA), 2 mM of MgCl2 ...
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bioRxiv - Cell Biology 2021Quote: ... the concentrated peak fractions were incubated with 5 μM Halo-Alexa488 (Promega) for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated with 5 μM coelenterazine h (Promega, Madison, WI, USA) in phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2022Quote: ... 5 μL of 50 μM stock solution of TMR dye (Promega G8251) was added to the concentrated culture ...
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bioRxiv - Microbiology 2022Quote: ... 1 complete protease inhibitor tablet EDTA-free and 5 µl DNaseI (Promega), lysed using a cell disruptor and the insoluble fraction removed by centrifugation (15,000 xg ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 μl of 5 ng μl−1 trypsin (sequencing grade, Promega®) in 50 mM AHC plus 1 mM CaCl2 were added to each well and incubated for 20 min at 4°C ...