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Citations for Promega :
601 - 650 of 1522 citations for 5 Methylsulfamoylmethyl 1H indole 3 carboxylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... An equal volume of reconstituted Caspase- Glo 3/7 Reagent (Promega, Madison, WI) was added to each sample and mixed by repeated pipetting ...
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bioRxiv - Neuroscience 2020Quote: ... The pCAG-DCC:TDTOMATO wildtype and missense mutant receptor constructs (1.764 µg) were also transfected into HEK293T cells cultured on acid-washed coverslips using FuGENE HD (Promega) in Optimem (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... and all subsequent steps were done as described in the manufacturer’s protocol (Maxwell 16 FFS Nucleic Acid Extraction System, Promega). DNA was eluted into a total volume of 80 μl elution buffer (EB ...
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bioRxiv - Biophysics 2021Quote: ... the C terminus of MC1R was fused with a 15-amino-acid polypeptide linker (GSSGGGGSGGGGSSG) and a LgBiT (Promega).
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bioRxiv - Molecular Biology 2022Quote: ... The human Gβ1 with a C-terminal 15-amino acid polypeptide linker was followed by a HiBiT (peptide 86, Promega), and the scFv16 was modified with an N-terminal GP67 signaling peptide and a C-terminal 8× histidine tag ...
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bioRxiv - Systems Biology 2023Quote: ... Digestion was performed overnight in 300 μL of 50 mM 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid (HEPES) buffer at pH 7.5 supplemented with endoproteinase Lys-C (Wako) and trypsin (Promega) at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Cap0 RNA was then purified using the same phenol:chloroform nucleic acid extraction as performed after the T7 transcription reaction (Promega). Cap0 RNA was determined to be at least 95% Cap0 RNA (m7GpppA ...
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bioRxiv - Cancer Biology 2024Quote: ... The dye was released from cell inserts using 30% acetic acid and absorbance was measured using GloMax Explorer (Promega) microplate reader at 590 nm as a readout for cell migration.
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bioRxiv - Cancer Biology 2021Quote: ... 5 μL of GoTaq® Probe qPCR Master Mix (Promega) and 0.5 μL of the appropriate TaqMan Assay (20X ...
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bioRxiv - Molecular Biology 2021Quote: ... and 5 μl of RNasin Plus RNase Inhibitor (Promega, N2611) and incubated for 2 hours at 4 °C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 5 U/μl GoTaq Flexi DNA Polymerase (Promega, WI, USA), and 1 μl of DNA template ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and 5 μL 2x buffer for T4 DNA Ligase (Promega) were mixed and incubated at 55°C for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... 20 μl of 5 ng/μl sequencing grade trypsin (Promega), 50mM AHC ...
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bioRxiv - Biochemistry 2020Quote: ... 5 mM MgCl2 with 0.3 µg/ul Acetylated BSA (Promega). All RNAs used were labelled with 5’ FAM (IDT ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 5 μL/well of CellTiter-Glo 2.0 reagent (Promega G9241) was added to the wells of the 384-well plate and allowed to equilibrate for 10 minutes before luminescence intensity was recorded on a Tecan SPARK plate reader (luminescence module ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM DTT and 5 μg/μl of trypsin (Promega)) for 3 min at 30°C ...
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bioRxiv - Genetics 2022Quote: ... 5-mins after the addition of furimazine NanoLuc substrate (Promega). A range of expression ratios of the NanoLuc and HaloTag containing constructs within the cells was achieved by varying the proportion of each plasmid during transfection ...
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bioRxiv - Plant Biology 2022Quote: ... and 5 μL of RNasin Plus RNase Inhibitor (Promega, N2611) and incubated for 2 h at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... 5 μl of M-MLV Reverse Transcriptase Buffer (Promega #M531A), 10 mM NTPs mixture and 200 U of MMLV (Promega #M170A ...
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bioRxiv - Biochemistry 2024Quote: ... 5 µl/well NanoBRET™ Nano-Glo® Substrate (Promega) and Extracellular NanoLuc® Inhibitor (Promega ...
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bioRxiv - Plant Biology 2023Quote: ... 10 µL of 5× Green GoTaq® Reaction Buffer (Promega), 1 µL of dNTP Mix (10 mM each of dTTP ...
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bioRxiv - Plant Biology 2023Quote: ... 10 µL of 5× Colorless GoTaq® Reaction Buffer (Promega), 1 µL of dNTP Mix (10 mM each of dTTP ...
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bioRxiv - Microbiology 2023Quote: ... The Nano-Glo® 5× reagent (furimazine substrate + buffer; Promega) was prepared and added to each sample ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 μL 5 ng/μL sequencing grade trypsin (Promega; V5111) prepared in 50 mM ABC supplemented with 1 mM CaCl2 were added to the gel pieces and incubated 30 min at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... A solution containing 5 μg of sequencing grade trypsin (Promega) in 100 mM triethylammonium bicarbonate (TEAB ...
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bioRxiv - Cell Biology 2023Quote: ... a mix was prepared consisting of 5 % PEG-8000 (Promega), 100 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Microbiology 2023Quote: ... 5 μL GoTaq Colorless Master Mix (Promega Corporation, Madison, Wisconsin) including 200 μM of each dNTP and 1 unit Taq DNA polymerase ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were then treated with 5 units of DNase (Promega) and incubated for an additional 30 min at 37°C before being loaded on a 15% urea-polyacrylamide gel ...
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bioRxiv - Biochemistry 2024Quote: ... 5 μL of the UDP-Glo luminescence detection reagent (Promega) were added and let incubate for 1 h at 25 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 μL of RNasin Plus RNAse inhibitor (Promega, Cat. #PRN2615) was added to the RNA ...
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bioRxiv - Cancer Biology 2024Quote: ... Lysis was performed using 5×Passive lysis buffer (Promega, #E1910), lysates were rocked in room temperature for 15 mins ...
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bioRxiv - Biochemistry 2024Quote: ... and 5 units/1 L RQ1 RNase-free DNase (Promega). Lysate was clarified via centrifugation at 17,800 x g for 30 minutes at 4°C.
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bioRxiv - Microbiology 2020Quote: ... The beads were then washed 3 times and incubated with 30ul lysis buffer (Promega). Then 15μl of the lysate was used to measure bound NanoLuc activity.
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bioRxiv - Molecular Biology 2021Quote: ... cells were transfected with 1 µg DNA and 3 µl FuGENE HD (Promega, E2311) in 150 µl Opti-MEM media according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Caspase 3/7 activity was measured using an Apolive-Glo kit (Promega, Madison, WI) and measured using a SpectraMax M5 (Molecular Devices ...
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bioRxiv - Biochemistry 2020Quote: ... and were harvested on day 3 by scraping into passive cell lysis buffer (Promega). Luciferase activity was then measured using a commercial dual luciferase assay kit (Promega) ...
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bioRxiv - Physiology 2020Quote: ... caspase 3/7 activity was measured using ApoLive-Glo Multiplex Assay (Promega, Madison, WI). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: ... Each sample was cut into 3 slices and in-gel digested with trypsin (Promega) as previously described15 ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested overnight at 37 °C by addition of 3 μg trypsin (Promega) and 2 μg LysC (Promega) ...
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bioRxiv - Cell Biology 2021Quote: ... Cell apoptosis was assessed using either the CaspaseGlo 3/7 assay (G8090, Promega, UK) according to the manufacturer’s instructions (N=4 replicates) ...
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bioRxiv - Molecular Biology 2021Quote: The 3’ UTRs were cloned into psiCheck-2 plasmid (Promega, USA, Cat. No. C8021) using the XhoI and NotI sites ...
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bioRxiv - Evolutionary Biology 2022Quote: Effector caspase activity was assessed using the Caspase-Glo 3/7 Assay System (Promega) following a previous protocol (Ronai et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... an equal volume of Caspase-Glo® 3/7 Assay System (Cat. No.G8090, Promega) was transferred into each well and incubated for 1 h and measured by a GloMax® 20/20 Luminometer ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and caspase-3/7 activity were measured using the ApoTox-Glo Triplex Assay (Promega) in a GloMax-Multi+ Reader (Promega) ...
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bioRxiv - Cancer Biology 2021Quote: ... Apoptotic activity was determined by the Caspase-Glo 3/7 luminescent assay (Promega, G8092), Annexin-V staining (Roche ...
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bioRxiv - Bioengineering 2020Quote: ... Cell apoptosis was measured using a Caspase-Glo 3/7 assay (Promega, Madison, WI).
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bioRxiv - Immunology 2020Quote: For cloning the USP18 3’UTR in the psiCHECK2 vector (Promega, Madison, WI, USA), the first 559nt of USP18 3’UTR were amplified from cDNA of HeLa S3 treated with IFN using the primers USP183UTR_XhoI_FW and USP183UTR_NotI_RV ...
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bioRxiv - Cancer Biology 2023Quote: ... Viability of organoids was determined after 3 days using CellTiterGlo reagent (Cat #G9683, Promega) and detected using a FluoStar Optima Plate Reader (21999 ...
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bioRxiv - Cancer Biology 2023Quote: ... survival was assessed using Cell Titer-Glo and Caspase 3/7 Glo assays (Promega) and analyzed at 24–48h post-treatments ...
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bioRxiv - Cell Biology 2023Quote: Caspase activity was assessed using Caspase-3/-7 Glo Luminescent Assay (Promega, Madison, WI) according to manufacturer’s instructions ...