Labshake search
Citations for Promega :
701 - 750 of 2957 citations for 1 1 Dimethylsila 11 Crown 4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2021Quote: ... were seeded in 96-wells white plate and caspase-1 activities were assessed using Caspase-Glo 1 Inflammasome Assay (Promega #G9951) according to manufacturer’s instructions.
-
bioRxiv - Biophysics 2020Quote: IVT reactions were incubated at 37°C for 45 min and then immediately transferred to an ice water bath and diluted 1:1 with cold Glo Lysis Buffer (Promega, #E2661). All samples were brought to room temperature and mixed with a 1:1 volume of nGlow solution (Promega ...
-
bioRxiv - Neuroscience 2020Quote: ... after which beads were washed with 1X High Salt Wash Buffer(50mM Tris-HCl pH 7.4, 350mM NaCl, 1%NP-40 and 1 unit/ul Promega recombinant RNAsin). In the last wash ...
-
bioRxiv - Neuroscience 2021Quote: ... For HiBiT detection, 25µL of HiBiT lytic reagent (1:25 substrate, 1:50 LgBiT) was added to the lysate (Promega, cat# N3050). For FFluc detection ...
-
bioRxiv - Biophysics 2021Quote: ... IVT reactions were incubated at 37°C for 45 min and then immediately transferred to an ice water bath and diluted 1:1 with cold Glo Lysis Buffer (Promega, #E2661). All samples were brought to room temperature and mixed with a 1:1 volume of nGlow solution (Promega ...
-
bioRxiv - Neuroscience 2021Quote: ... rabbit anti-T-Cofilin 1:2000 (Bamburg lab, 1439 or Cell Signalling, 5175) and mouse anti-β3-tubulin 1:2000 (Promega, G7121) diluted in 1%BSA/PBS and incubated overnight at 4ºC ...
-
bioRxiv - Molecular Biology 2023Quote: Predigestion of bound proteins was performed by addition of 200 ng / µl of a 1:1 LysC (FUJIFILM Wako Pure Chemical Corporation, 125-02543) trypsin (Trypsin Gold, Mass Spec Grade, Promega, V5280) mix ...
-
bioRxiv - Microbiology 2023Quote: The assay was adapted from 15 where Hyp1-Nluc schizonts at 1% hematocrit and 1-2% parasitaemia were lysed in 1x NanoGlo buffer (Promega, USA) within a 96-well flat-bottom plate and parasite lysates were added to compounds and incubated for 10 minutes at 37°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... washed with PBS and inflammasome activation was assessed using qPCR or inflammasome caspase-1 activity was measured using Caspase-Glo® 1 inflammasome assay kit (G9951; Promega) according to manufacturer’s instructions.
-
bioRxiv - Immunology 2024Quote: ... The membrane was then washed thrice with 1X PBST and incubated for 1 hr with HRP conjugated secondary antibody (1:5000; Promega #w4018). After washing with PBST the membrane was developed using the enhanced chemiluminescence reagent (Bio-Rad Laboratories ...
-
bioRxiv - Plant Biology 2022Quote: ... First-strand cDNA was synthesised using 1 µg RNA and 500 ng µl-1 Oligo(dT)15 primer (Promega, Southampton, UK), denatured at 65°C for 5 min ...
-
bioRxiv - Physiology 2024Quote: ... further steps were performed according to the manufacturer’s protocol using proteases trypsin and Lys-C (2 h at 47° C, 1:15 and 1:30, respectively, Promega, USA). Peptides (20 μg ...
-
bioRxiv - Cell Biology 2024Quote: ... The HMEC cell lines stably expressing doxycycline-inducible Rb variants were generated by transfecting cells with 1 µg of doxycycline- inducible plasmid and 1 µg of PiggyBac Transposase plasmid using the FuGene HD reagent (Promega E2311). Zeocin (300 µg/ml ...
-
bioRxiv - Microbiology 2024Quote: ... Cells were then fixed in 1% paraformaldehyde/PBS for 1 minute and washed with PBS and with 2X SSC (Promega, V4261), followed by Incubation in 50%formamide/2X SSC (pH7-7.5 ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1 ml ice-cold wash buffer 1 (1x PBS with 2 % BSA, 1 mM DTT and 0.5 U/µl RNasin® Plus Ribonuclease Inhibitor - Promega #N2615) was added and cells were spun at 500 g for 3 min at 4 C ...
-
bioRxiv - Physiology 2024Quote: ... and hydrolyzed into the S-Trap column using trypsin and Lys-C (2 h at 47 °C, 1:15 and 1:30, respectively, all Promega, USA) in 40 μl 50 mM triethylammonium bicarbonate according to the manufacturer’s recommendations ...
-
bioRxiv - Cancer Biology 2024Quote: ... Nuclei were washed with Wash Buffer three more times and nuclei pellets were resuspended in Diluted Nuclei Buffer (1x Nuclei Buffer, 10x genomicsTM, supplemented with 1 mM DTT and 1 U/uL RnaseIn, Promega©) for counting and scMulti library preparation ...
-
bioRxiv - Molecular Biology 2020Quote: ... 4 μg of RNase A (Promega), and 40 units of RNase ONE (Promega ...
-
bioRxiv - Cell Biology 2023Quote: ... 4 µl RT buffer 5x (Promega), 2.5 μl dNTPs 2.5 mM ...
-
bioRxiv - Cancer Biology 2024Quote: ... 4 µg Trypsin/LysC mix (Promega) were added and the sample incubated for 4 h at 37°C ...
-
bioRxiv - Cancer Biology 2024Quote: ... 4 µg Trypsin/LysC mix (Promega) were added and the sample incubated for 4 h at 37°C ...
-
bioRxiv - Molecular Biology 2021Quote: ... secondary antibody staining was performed for 1 h at room temperature using anti-rabbit HRP or anti-mouse HRP secondary antibodies (Promega, 1:10,000) in blocking buffer ...
-
bioRxiv - Synthetic Biology 2020Quote: ... 1:2000) and the bound primary antibodies were detected by a HRP-conjugated goat anti-rabbit secondary antibody (W401B, Promega; 1:2000). Membranes were developed and visualized using the Pierce™ ECL immunoblotting substrate (ThermoFisher Scientific).
-
bioRxiv - Cell Biology 2022Quote: ... 30 mM Tris-HCl (pH 7), 1% Triton X-100, 1% NaDOC, 100 μg/mL cycloheximide (Applichem, Germany) and 30 U/mL RNase Inhibitor (Promega, United States)] ...
-
bioRxiv - Biochemistry 2022Quote: ... The proteins were digested with 1 μg lysyl endopeptidase (LysC) (FUJIFILM Wako Pure Chemical Corporation) and 1 μg trypsin (Promega, Tokyo, Japan) overnight at 37°C on a shaking incubator ...
-
bioRxiv - Microbiology 2021Quote: ... The CaptureSelect Biotin anti-C-tag Conjugate (LifeTechnologies #7103252100; 1/4000) was mixed with Streptavidin Alkaline Phosphatase (Promega #V5591; 1/1000 dilution) for 10 minutes before use ...
-
bioRxiv - Microbiology 2021Quote: ... The reaction mixture was prepared into 96-well plates as follows: 1 μl of 1 mM amino acid mixture minus methionine (Promega, Cat: L9961), 35 μl of rabbit reticulocyte lysate ...
-
bioRxiv - Cancer Biology 2020Quote: ... and diluted to 1 M urea with 200 mM ammonium bicarbonate for trypsin digestion (1 µg, 37°C, 8 hr, Promega cat # V5113). After digestion ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK293T cells were co-transfected in a 1:1 ratio with either human D1 or D2Long receptor and a split-luciferase based cAMP biosensor (GloSensor, Promega, Durham NC). The next day ...
-
bioRxiv - Immunology 2020Quote: Caspase-1 and Caspase 3/7 activities were measured using Caspase-Glo® 1 and Caspase-Glo® 3/7 assay (Promega) respectively ...
-
bioRxiv - Microbiology 2020Quote: ... The protein mixture was diluted four times and digested with LysC at 1:50(w/w) for four hours and then with Trypsin at 1:40 (w/w) (Promega, http://www.promega.com/) overnight.
-
bioRxiv - Molecular Biology 2022Quote: ... we prepared a master mix containing the membrane-permeable Nluc substrate and a membrane-impermeable Nluc inhibitor using a 1:1000 dilution of Extracellular NanoLuc Inhibitor and a 1:333 dilution of NanoBRETTM Nano-Glo Substrate into PBS (Promega, Madison, WI). Aliquots of the Nluc substrate/inhibitor master mix (100 µl ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The proteins were digested with 1 μg of lysyl endopeptidase (LysC) (FUJIFILM Wako Pure Chemical Corporation) and 1 μg of trypsin (Promega, Tokyo, Japan) overnight at 37 °C in a shaking incubator ...
-
bioRxiv - Biochemistry 2024Quote: ... In-solution digestion was performed with LysC-Trypsin mix (1:100 enzyme: protein ratio) and trypsin (Promega; 1:50 enzyme: protein ratio). Peptides were desalted on C18 stage tips ...
-
bioRxiv - Cell Biology 2023Quote: ... and 10μg of pLKO.1-mCherry-scrambled shRNA or pLKO.1-mCherry-IRE1α shRNA using FuGENE-HD reagent (Promega, Madison, WI, USA). After 24 h of transfection ...
-
bioRxiv - Biochemistry 2022Quote: ... secondary antibody staining was performed for 1 h at room temperature using antirabbit HRP or anti-mouse HRP secondary antibodies (Promega,1:10,000) in blocking buffer ...
-
bioRxiv - Molecular Biology 2024Quote: ... The crude nuclei were pelleted and resuspended in 400 μl nuclei lysis buffer (50 mM Tris-HCl pH 8.0, 10 mM EDTA, 1% SDS, 1× EDTA-free Proteinase Inhibitor, and 1000 U RNase I Promega, Madison, WI, USA). Supernatant obtained after sonication and centrifugation steps were digested with DNase I for 20 min at 37°C ...
-
bioRxiv - Neuroscience 2024Quote: ... blots were incubated for 1 h at room temperature with the corresponding horseradish peroxidase-conjugated antibody (1:2000; Promega, Madison, WI, USA) and washed again with TBS-T ...
-
bioRxiv - Plant Biology 2024Quote: ... columns were pre-equilibrated with 5 CV of 0.1 M NH4CO3 and then loaded with 1 CV of 0.1 M NH4CO3 containing 1 µg/ml trypsin gold (Promega V5280, Promega, USA). The flow-through containing the digested peptides of S-nitrosated proteins (elution 1 ...
-
bioRxiv - Cell Biology 2024Quote: ... clarified by centrifugation (500 x g, 5 min, at 15°C) and mixed 1:1 with 2× passive lysis buffer (Promega cat #E1941). Cell monolayers were washed in PBS and lysed in 1× passive lysis buffer ...
-
bioRxiv - Microbiology 2020Quote: ... 0.3% xylose and 1% low melting temperature agarose (Promega) on slides ...
-
bioRxiv - Developmental Biology 2021Quote: ... and 300 U mL-1 RNase inhibitor (Promega, N2112S). The mixture was subsequently incubated with 20 µL of Dynabeads protein-A (Life Technologies ...
-
bioRxiv - Developmental Biology 2021Quote: ... and 300 U mL-1 RNase inhibitor (Promega, N2112S). After the addition of 50 µL Dynabeads protein-A (Life Technologies ...
-
bioRxiv - Neuroscience 2022Quote: ... anti-b-111-tubulin (mouse, Promega G712A, 1:500), Cleaved caspase-3 (rabbit ...
-
bioRxiv - Microbiology 2022Quote: ... 1 µL of RNAse inhibitor (40 U/µl, Promega) was added to stop the reaction ...
-
bioRxiv - Molecular Biology 2021Quote: ... rabbit anti-NLuc (kind gift of Promega, 1:1000) and b-actin-peroxidase (Sigma ...
-
bioRxiv - Molecular Biology 2021Quote: ... rabbit anti-NLuc (kind gift of Promega, 1:100). The following Alexa Fluor secondary antibodies were used ...
-
bioRxiv - Developmental Biology 2022Quote: ... Anti–β-galactosidase monoclonal mouse antibody (Promega, 1:500) was co-incubated with anti-mCherry polyclonal rabbit antibody (Bio Vision ...
-
bioRxiv - Biochemistry 2020Quote: ... 1x reaction buffer (from Promega (containing 1 mM Co2+) for Supplementary Fig ...
-
bioRxiv - Bioengineering 2020Quote: ... and 1 unit T4 ligase with associated buffer (Promega). The following thermocycler program was run ...