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Citations for Promega :
7301 - 7350 of 8253 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... cells were co-transfected with the equimolar amount of an empty pGEM®-T vector (Promega, WI USA) with the size of around 3 kb ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were seeded in 6-well plates at 400,000 cells/well and transfected after 24 hours using 1μg of plasmid and Fugene6 (#E2691, Promega) according to the manufacturer’s instructions ...
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The Ets protein Pointed P1 represses Asense expression in type II neuroblasts by activating TaillessbioRxiv - Developmental Biology 2021Quote: ... Proteins were expressed from the pcDNA™3.1/His construct by the TNT® T7 Quick Coupled Transcription/Translation kit (Catalog #L1170, Promega Co., Madison, WI) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... was reverse transcribed with a GoScript™ Reverse Transcriptase cDNA reverse transcription kit according to the manufacturer’s instructions (Promega Corporation, an affiliate of Promega Biotecnologia do Brasil, Ltda). Primers were designed and synthesized by IDT-DNA (San Diego ...
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bioRxiv - Microbiology 2022Quote: ... The resulting suspension was then centrifuged at 14,000 rpm at 4°C to remove cellular fragments and the genomic DNA finally was extracted using the Wizard® Genomic DNA Purification Kit reference A1125 (Promega, Madison, WI, USA) following the bulla guidelines ...
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bioRxiv - Microbiology 2022Quote: ... then purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) and quantified using a Quantus™ fluorometer (Promega, Madison, WI, USA).
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bioRxiv - Microbiology 2020Quote: ... faginata MAT1-1 isolate using a CTAB-chloroform DNA extraction method (van Diepen et al. 2017) and a MAT1-2 isolate using a Wizard® kit (Promega, Madison, WI, USA) and suspended in 75 µl Tris-EDTA (TE ...
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bioRxiv - Molecular Biology 2020Quote: ... purified amplicons from each single-plex TaqMan® qPCR were cloned into a plasmid vector using the pGEM®-T Easy Vector System I kit (Cat.# A1360, Promega, Walldorf, Germany) and One Shot® TOP10 (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... AccuPrep® Plasmid Mini Extraction Kit (Bioneer Corporation, Republic of Korea) and Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, USA) were used for plasmid and PCR product extraction and purification ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from each sample taken from captured birds and carcasses using the Wizard Genomic DNA Purification kit (Promega Corp., Madison, Wisconsin, USA) according to the manufacturer’s instructions and eluted in 125 µl of DEPC-treated water ...
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bioRxiv - Plant Biology 2020Quote: Degenerate primers specific to each gene were designed using Primer3plus software [https://primer3plus.com/] for all 17 identified CKX genes following the guidelines for primer design provided by the PCR amplification kit manufacturer (Promega Corporation, USA; Table S2). PCR amplification of the identified CKX GFMs was performed using the reaction kits selected based on the gene size ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... One microgram of total RNA from each sample was reverse transcribed into first-strand cDNA by using the M-MLV kit (Promega Corporation, Madison, WI, USA) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The value of relative light unit of luciferase was determined using the PicaGene Luminescence Kit (TOYO B-Net Co., Ltd., Tokyo, Japan) and GloMax Navigator System G2000 (Promega Corporation, Madison, WI, USA), according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA from the bacterial strains used as positive controls was extracted using the Wizard® Genomic DNA Purification Kit (Promega, Madison, WI, U.S.) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Cell pellets resulting from later experiments (optimization of medium composition and fiber- and drug-testing) were pre-processed using the Maxwell® HT 96 gDNA Blood Isolation Kit (Promega AG, Dübendorf, Switzerland) combined with a FastPrep homogenizer (FastPrep-24TM ...
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bioRxiv - Microbiology 2022Quote: ... Colonies were collected and subjected to total DNA extraction using the Maxwell® 16 Tissue DNA Purification Kit in the Maxwell 16 Research Instrument (Promega, Madison, WI, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... DNA fragments such as PCR products or DNA fragment as an agarose gel slices were purified using the Wizard® SV Gel and PCR Clean-Up System kit from Promega (Madison, WI, USA). DNA was digested with the appropriate restriction endonucleases (FastDigest ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: We extracted total RNA by combining a TRIZOL-chloroform approach with a spin column kit (SV Total RNA Isolation System, Promega (Peuß et al., 2016)) ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA isolation was performed by Maxwell® 16 Instrument configured with the LEV High Strength Magnetic Rod and Plunger Bar Adaptor and Maxwell® 16 LEV simplyRNA Purification Kit (PROMEGA, WI, The US) by following the supplier’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Primers flanking the upstream and downstream regions of each gene of interest were amplified from PA14 genomic DNA (Promega Wizard Genomic DNA Purification Kit) and extracted with GeneJet Gel Extraction Kit (ThermoFisher) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... fruticulosa plants hydroponically cultivated under 0 mM or 150 mM NaCl was extracted using the Maxwell plant RNA kit (Promega Corporation, Madison, WI, USA) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA from individual calli was extracted using 160 mg of frozen powder with the Promega ReliaPrep™ RNA Tissue Miniprep System kit (Promega, France, Ref. Z6112) following the Promega - ReliaPrep™ RNA Tissue Miniprep System protocol ...
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bioRxiv - Biochemistry 2024Quote: ... DNA was isolated from the fecal slurries using a bead-beating procedure coupled with the customized MaxWell 16 Tissue LEV Total RNA Purification Kit (XAS1220; Promega Biotech AB, Stockholm, Sweden). DNA isolates underwent triplicate polymerase chain reaction (PCR ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and DNA was extracted using a Maxwell 16 Instrument in combination with the Maxwell® 16 Tissue LEV Total RNA Purification Kit Custom (XAS 1220) (Promega, Madison, WI, USA) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were lysed 24 hours after transfection and the lysates were subjected to dual-luciferase reporter (DLR) assay (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was isolated from cells or murine liver tissue using the Relia Prep RNA Tissue Miniprep System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Hela cells were co-transfected with CRISPR-Cas9 plasmids and a plasmid conferring puromycin resistance using FuGENE (Promega, E2693). 24 h after transfection ...
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bioRxiv - Cell Biology 2020Quote: ... and harvested at varying timepoints by resuspending the cells in 50-100 μL of 1X Passive Lysis Buffer (Promega). Inductions were staggered so that all timepoints could be harvested simultaneously ...
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bioRxiv - Immunology 2021Quote: ... firefly and Renilla reniformis luciferase activities from lysed cells were determined using the dual-luciferase reporter assay system in accordance with the manufacturer’s protocol (Promega). Data are expressed as the relative firefly luciferase activities normalized to the activity of the R ...
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bioRxiv - Developmental Biology 2020Quote: His-P150-CC1 (Courtois et al., 2012) was expressed in and purified from BL21(DE3)pLysS competent cells (Promega) as previously described (Courtois et al. ...
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bioRxiv - Genetics 2022Quote: ... Firefly and Renilla luciferase activity of cell lysates were sequentially assayed using the Dual-Luciferase Reporter Assay System (Promega) on a GloMax Explorer luminometer (Promega) ...
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bioRxiv - Molecular Biology 2020Quote: ... The cells were then transfected with mCherry fusions of actin or Lifeact variants using the FuGENE transfection reagent (Promega). The cells were grown for a further 24 h and transferred to an LSM 800 microscope (Zeiss ...
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bioRxiv - Molecular Biology 2020Quote: Gently re-suspend cells in 100 μl of 3% Glyoxal fixation solution with 1:25 RNasin Plus (Promega N261B) and incubate for 15 minutes on ice.
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bioRxiv - Bioengineering 2022Quote: ... The cell viability was investigated through the LDH dependent CytoTox 96 Non-radioactive Cytotoxicity assay (#G1780, Promega, Dübendorf, Switzerland.) and the ATP dependent CellTiter-Glo® 2.0 Cell Viability Assay (#G9241 ...
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bioRxiv - Immunology 2022Quote: Replication competent HIV were produced by transfection of HEK293T cells in T150 flasks using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... The trophocidal activity of compounds were assessed using the CellTiter-Glo 2.0 luminescent cell viability assay (Promega, Madison, WI). In brief ...
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bioRxiv - Molecular Biology 2021Quote: ... All the clones were transfected into the HEK-293T cells at 90% confluency using FuGene 6 as transfection reagent according to the manufacturer’s instructions (Promega). dsiRNAs targeting Rpp30 (10 nM ...
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bioRxiv - Microbiology 2020Quote: ... cell viability was assessed by applying 20 µl of CellTiter 96® AQueous One Solution Reagent (Promega, Madison WI) to each well and incubating for 2 h at 25°C ...
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bioRxiv - Microbiology 2021Quote: ... 5 μL of DNA or 5 μL of cell lysate were mixed with 12.5 μL of GoTaq Colorless Master Mix (Promega), 1 μL of forward and reverse primer (5 μM ...
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bioRxiv - Immunology 2021Quote: ... 16HBE were seeded at 10,000 cells/well of a 96-well flat-bottom dish and transfected using FuGENE HD (Promega).
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bioRxiv - Synthetic Biology 2021Quote: Cells for were transferred to opaque-bottomed white plates (Grenier-Bio #655073) and then assessed for luminescence (Promega #E1501) per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... The pCAG-DCC:TDTOMATO wildtype and missense mutant receptor constructs (1.764 µg) were also transfected into HEK293T cells cultured on acid-washed coverslips using FuGENE HD (Promega) in Optimem (Gibco ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293T cells were co-transfected with pEF-BOS-based expression vectors for hXkr8 and hBSG using Fugene○R6 (Promega) and cultured for 2 days ...
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bioRxiv - Bioengineering 2020Quote: ... the Nbs-virus mixture was removed from Vero E6 cells followed by 500 μL of DMEM with 2% FBS and 0.9% carboxymethyl cellulose (Promega) was overlaid ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were resuspended in 4 ml of Blob100 buffer plus 80 units/ml RNasin Plus (Promega, Flitchburg, WI, USA) and lysed under liquid nitrogen in a 6870 Freezer Mill (Spex ...
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bioRxiv - Microbiology 2021Quote: The HEK 293T cell viability in the presence of compounds was measured using a CellTiter-Glo 2.0 (Catalogue number G9241, Promega) based on the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... reversely transfected into LNCaP or VCaP (treated with DHT or ETH) cells with a Renilla Luciferase control plasmid pGL4.75 [hRluc/CMV] (Promega) by using X-treme GENE HP DNA Transfection Reagent (Roche) ...
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bioRxiv - Cancer Biology 2020Quote: Cell proliferation assays were carried out by measuring the production of ATP using the CellTiter-Glo luminescent assay (Promega) as per the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were lysed by a 15-min incubation at room temperature with passive lysis buffer (E1941, Promega, Madison, USA). Cell lysates (30 μL ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were collected 24 h after transfection and luciferase activities were measured with Dual-Luciferase Reporter Assay System (Promega) according to manufacturer’s instructions.