Labshake search
Citations for Promega :
7051 - 7100 of 8253 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: Healthy young leaves from each line were collected and genomic DNA was extracted using a Maxwell RSC Plant DNA Kit (Promega, Madison, WI, USA). The presence or absence of the 5.46-kb fragment containing DAI was confirmed by PCR (primers 1 and 2) ...
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bioRxiv - Cell Biology 2022Quote: ... was used as a template in PCR reactions and amplifications were performed with the GoTaq®G2 Flexi DNA Polymerase kit (Promega, Southampton, UK). Cycling parameters were one cycle of 95°C for 5 minutes for initial denaturation followed by touch-down cycling for the first 14 cycles ...
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bioRxiv - Microbiology 2020Quote: ... genomic DNA was isolated from the corresponding BKE (Bacillus Knockout Erythromycin collection - (Koo et al., 2017)) strains using the Wizard® Genomic DNA Purification Kit (Promega, Madison, WI). Of note ...
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bioRxiv - Cancer Biology 2020Quote: ... Lipofectamine 3000 was used to co-transfect HEK-293T cells with wild-type and mutant luciferase reporter vectors as well as P51-ETS1-NC or P51-ETS1.Relative luciferase activity was expressed as the ratio of firefly luciferase activity to sea kidney luciferase activity using a dual luciferase reporting kit (Promega, Madison, Wisconsin, USA) after 48 h.
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bioRxiv - Immunology 2020Quote: ... were verified by Sanger sequencing and used as DNA templates in the T7 TNT in vitro transcription/translation kit (Promega, Madison, WI; #L1170) using [35S]-methionine (PerkinElmer ...
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bioRxiv - Pathology 2020Quote: ... cDNA synthesis from the mRNA was performed using a Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) kit (Promega, Madison, Wisconsin, USA) in a Mastercycler® Gradient (Eppendorf ...
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bioRxiv - Microbiology 2020Quote: ... dahliae strain JR2 was isolated from tomato roots seven days after root dip inoculation and following five days of in vitro growth in soil extract and potato dextrose broth (PDB) using the Maxwell® 16 LEV Plant RNA Kit (Promega, Madison, USA). Real-time PCR was performed as described previously17 to determine the expression of effector genes relative to VdGAPDH with primer pairs as shown in Extended data Table 6.
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bioRxiv - Microbiology 2021Quote: Apical and basolateral samples were used to quantify LDH release in RV-C15-infected HAE with the CytoTox 96® Non-Radioactive Cytotoxicity Assay kit (#G1780; Promega, Madison, WI) following the manufacturer’s protocol.
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bioRxiv - Microbiology 2021Quote: LDH activity and percentage cell cytotoxicity in rabbit lungs (n=3 per group per time point) were determined using the LDH-Glow cytotoxicity kit as per the manufacturer’s instructions (Promega Corporation, Madison, WI, USA). Luminescence was recorded using a GloMax plate reader ...
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bioRxiv - Pathology 2021Quote: ... we first prepared cDNAs from a total RNA sample isolated from an RSV-infected rice plant using the Reverse Transcription Kit as instructed (Promega, Madison, WI, USA). We then amplify the full-length RSV RNA3(- ...
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bioRxiv - Biochemistry 2021Quote: ... Amplified PCR products were visualized by blue light illumination and the 504/505 bp bands were excised and the amplified PCR product purified using the Wizard SV Gel and PCR Clean-Up kit (Promega, Madison, WI, USA). Extracted DNA was Sanger sequenced at the University of Michigan Advanced Genomics Core and analyzed for presence of the single base pair deletion using DNAStar LaserGene SeqMan Pro 15 ...
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bioRxiv - Biochemistry 2021Quote: The kinase activity of the FLT3-ITD protein was measured by using ADP-Glo™ Kinase Assay Kit (Promega, Madison, Wisconsin, the US). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: Genomic DNA was extracted from FFPE tumor tissue samples of 18 patients within the cohort using the automated Maxwell system with the Maxwell 16 FFPE Plus LEV DNA Purification Kit (Promega, Madison, WI, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: RNA was extracted from FFPE tissue samples using the automated Maxwell system with the Maxwell 16 LEV RNA FFPE Kit (Promega, Madison, WI, USA), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... All RACE-PCR products were purified using GeneJET Gel Purification Kit (Fermentas, Hanover, MD, USA) and ligated into pGEM-T vector (Promega, Madison, WI, USA) and transformed into competent JM-109 Escherichia coli cells (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... 24 hours later cells were harvested into 125 μl reporter lysis buffer and luciferase activity and β-galactosidase levels measured using a luciferase assay kit (Promega, Madison, WI) and a luminometric β-galactosidase detection kit (Takara Bio ...
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bioRxiv - Microbiology 2020Quote: Samples from the human subject were extracted with a DNA extraction protocol adapted from the Maxwell RSC Buccal Swab DNA kit (Catalogue number AS1640: Promega Corporation, Madison WI). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... The relative light unit value of luciferase was determined using the PicaGene Luminescence Kit (TOYO B-Net Co., Ltd., Tokyo, Japan) and GloMax Navigator System G2000 (Promega Corporation, Madison, WI), according to the manufacturer’s protocol.
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bioRxiv - Physiology 2023Quote: Glutathione content was measured under normoxic conditions and after exposure to 30 min and 6 h hypoxia using the GSH-Glo Assay Kit (Promega, Madison, WI, USA) according to manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... DNA was purified from the samples using the Maxwell 16 Tissue LEV Total RNA purification Kit custom-adapted for DNA extraction (cat no. AS 1220, Promega, Madison, Wisconsin, USA) in a Maxwell 16 MDx instrument (Promega) ...
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bioRxiv - Immunology 2023Quote: ... 1.5 μL of N2 primers and probe (2019-nCov CDC EUA Kit, Integrated DNA Technologies) and 10 μl of GoTaq 1-Step RT-qPCR (Promega, Madison, WI, USA). Thermal cycling was performed at 50°C for 15min for reverse transcription ...
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bioRxiv - Systems Biology 2022Quote: ... Resistance against ribociclib was detected by the alteration of the dose–response curve measured using CellTiterGlo Chemiluminescent Kit (Promega Corporation, Cat. No.: G7573).
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bioRxiv - Neuroscience 2024Quote: NAD and NADP levels were measured using the commercially available kits NAD/NADH-Glo Assay and NADP/NADPH-Glo Assay (Promega G9071, Promega G9081), respectively ...
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bioRxiv - Microbiology 2024Quote: ... Six presumptive transconjugant colonies were randomly selected and grown overnight in 1 mL of LB prior to DNA extraction using the Wizard® Genomic DNA Purification kit (Promega, Madison, WI). The AMR genotypes of transconjugant colonies were determined by polymerase chain reaction (PCR ...
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bioRxiv - Microbiology 2023Quote: ... globosa gut isolates (KCTC 37188 and KCTC 37189) and skin isolates (KCTC 27541 and KCTC 27777) was extracted using the Wizard HMW DNA Extraction Kit (Promega, Madison, WI, USA), according to the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2024Quote: ... RNA was isolated from frozen harvested materials using the SV Total RNA isolation kit with on-column DNA digestion (Promega, Madison, WI, USA). The purity of all RNA samples was confirmed using a NanoDrop2000 instrument (Thermo Scientific) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... senegalensis DNA was extracted from muscle tissues in thoraxes using Maxwell® 16 LEV Plant DNA Kit (Cat. No. AS1420, Promega, WI, USA). Details on extraction and library preparation protocols are provided in the Supporting Text 1.
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bioRxiv - Cancer Biology 2023Quote: gDNA extraction from formalin-fixed paraffin-embedded (FFPE) tissue sections was carried out using Maxwell 16 FFPE plus LEV DNA purification kit (AS1720; Promega, Madison, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... coronary effluent was collected for detection and quantification of lactate dehydrogenase (LDH) using the Promega luminescent LDH-Glo 105TM Cytotoxicity Assay kit following manufacturer protocol (Promega, Madison, WI, USA).
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bioRxiv - Neuroscience 2023Quote: ... was reverse transcribed with a GoScriptTM Reverse Transcriptase cDNA reverse transcription kit according to the manufacturer’s instructions (Promega Corporation, an affiliate of Promega Biotecnologia do Brasil, Ltda). Primers were designed and synthesized by IDT-DNA (San Diego ...
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bioRxiv - Microbiology 2022Quote: ... The viral RNAs were extracted from the homogenized lung tissue samples (10% w/v) and nasal wash samples using the Maxwell® RSC Viral Total Nucleic Acid Purification Kit (Promega, Madison, WI). The whole genomes of SARS-CoV-2 used in this research were amplified using a modified ARTIC protocol with several primers replaced or added (57 ...
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bioRxiv - Cell Biology 2022Quote: ... PCR products were purified with the Nucleospin gel and PCR clean-up kit (Macherey Nagel) and transcribed in vitro using T7 RNA polymerase (RiboMAX system; Promega, Madison, WI, USA). RNA was purified from the sample using the Nucleospin RNA clean-up kit (Macherey Nagel) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Total RNA was extracted from the antennae (50 individuals) of soldiers and workers using an SV Total RNA extraction kit (Promega, Madison, WI, USA). The amount of RNA and DNA in each sample was quantified using a Qubit fluorometer (Life Technology ...
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bioRxiv - Molecular Biology 2023Quote: The DNA was extracted using the Promega PCR Cleaning Kit (Wizard® SV Gel and PCR Clean-Up System, Promega Cat.-No. A9285) as indicated in the manual ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were extracted from 2% agarose gel and purified using the AxyPrep DNA Gel Extraction kit (Axygen Biosciences, Union City, CA, USA) according to the manufacturer’s instructions and quantified using Quantus™ Fluorometer (Promega, Madison, WI, USA). The Illumina MiSeq platform (Illumina Inc. ...
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bioRxiv - Genomics 2023Quote: ... The resulting DNA was resuspended in 1× IDTE buffer (Integrated DNA Technologies, Coralville, IA, USA) and quantified using the QuantiFluor One dsDNA kit (Promega, Madison, WI, USA). The HiFi reads were obtained using the SMRT Cell 8M and the PacBio Sequel II instrument ...
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bioRxiv - Developmental Biology 2023Quote: ... DNAs were purified using the PureYield Midiprep kit and were linearized before in vitro synthesis of anti-sense RNA probes using T7 or Sp6 polymerase (Promega, #P2077 and #P108G), RNAse inhibitor and dig-labeled rNTPs (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... and transferred to 96-well black plate to be treated with LARII and Stop & Glo components according to protocol of the Dual-Luciferase® Reporter Assay Kit (Promega CN: E1910). Luminescence was measured using a microplate reader (TECAN infinite M200pro) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we digested tissues using Proteinase K enzyme and used a Maxwell® Rapid Sample Concentrator Instrument with Maxwell® 16 Tissue DNA Purification Kit (Promega Corporation). We used 34 cycles polymerase chain reaction (PCR ...
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bioRxiv - Microbiology 2024Quote: ... for intra-subcellular (i.e., vacuolar) conversion of glutamine (Gln) to glutamate (Glu) assay using Glutamine/Glutamate-Glo Assay kit (Promega, Madison, WI; Cat. # J8021) following kit’s protocol ...
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bioRxiv - Systems Biology 2024Quote: ... the potential cytotoxicity of condition media was tested on an aliquot of medium collected from each technical replicate using the CytoTox 96® Non-Radioactive Cytotoxicity Assay kit (Promega, Cat # G1780).
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bioRxiv - Biochemistry 2024Quote: ... and tumors were briefly washed with FluoroBrite DMEM prior to submerging in 200 µL furimazine-FluoroBrite (diluted 1:200 from the Promega Nano-Glo kit). L713F-nLuc signals were then immediately measured using nLuc-specific filter settings (470 ± 40 nm) ...
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bioRxiv - Cell Biology 2024Quote: ... BP1425-500) at 37 °C and the plasmid was isolated from single colonies using the PureYield Plasmid Midiprep kit (Promega, Cat no. A2495), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The amplified product was used as a template to synthesize dsRNA using a RiboMAX Large Scale System-T7 Kit (Promega, Madison, WI, USA). The dsRNA was then isolated and purified ...
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bioRxiv - Cell Biology 2024Quote: Apoptosis was assessed by measuring caspase 3/7 activity using a Caspase-Glo 3/7 Assay Kit (Promega, Southampton, UK, Cat. No. G8091), according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... Resistance against chemotherapy was detected by the alteration of the dose–response curve measured using CellTiterGlo Chemiluminescent Kit (Promega Corporation, Cat. No.: G7573). Cell lines were confirmed to be mycoplasma-negative using the Mycoalert PLUS Mycoplasma detection kit (Lonza ...
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bioRxiv - Cancer Biology 2024Quote: ... collateral sensitivity against each drug was detected by measuring the viability of each line using CellTiterGlo Chemoluminescent Kit (Promega Corporation, Cat. No.: G7573). Relative viability of each resistant cell under each condition was normalized to parental sensitive cells under the same condition and were subjected to log2-transformation ...
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bioRxiv - Developmental Biology 2024Quote: ... the ovaries were dissected and pretreated following a previously described procedure[45] and then subjected to the TUNEL assay to observe apoptosis by using an apoptosis detection kit (Promega, USA, Cat# G3250). The experimental procedures were performed according to the manufacturer’s instructions with slight modifications described previously [45] ...
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bioRxiv - Developmental Biology 2024Quote: ... The level of reduced GSH was quantified indirectly by subtracting oxidized GSH (GSSG) from total GSH using commercial kit GSH/GSSG-Glo™ Assay (Promega, Madison, WI) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were transfected with 750 ng of the pMXs-IP vectors with 2.3 μl FuGENE 6 (Promega, E2691). The culture medium was replaced with fresh DMEM with 10% FBS and C2C12 cells were seeded at a concentration of 1×105 cells/well in a 12-well plate on day 3 ...