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Citations for Promega :
651 - 700 of 4304 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... overnight at 37°C or trypsin (Promega) for 1 hr at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... with the C-terminal HiBiT-Tag (Promega), using the In-Fusion cloning kit ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing-grade trypsin/Lys-C mixture (Promega) was added at 1:50 protease to protein ratio before incubation at 37°C for 16 hours ...
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bioRxiv - Immunology 2023Quote: ... Initial digestion with 15μg Lys-C (Promega) was performed by incubation 2h RT ...
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bioRxiv - Neuroscience 2023Quote: Protein-protein interaction was analyzed using the NanoBit Protein:Protein Interaction (PPI) System (Promega) according to the manufacturer’s instructions ...
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Vasohibin1, a new IRES trans-acting factor for sequential induction of angiogenic factors in hypoxiabioRxiv - Cell Biology 2019Quote: Eluted protein samples from BIA experiment were digested in gel with 1 µg of trypsin (sequence grade, Promega) at 37°C OVN ...
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bioRxiv - Microbiology 2021Quote: ... sample-filters were incubated in a wet-cell adding 1:50 w/w trypsin: protein ratio (Promega, V5111) solubilized in 120 µl 50 mM TEAB ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested by incubation with either sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin), chymotrypsin sequencing grade (1/50 ...
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bioRxiv - Neuroscience 2022Quote: ... Protein bands were detected with horseradish peroxidase (HRP)-conjugated rabbit or mouse secondary antibody [1:1,000 dilution] (Promega). The chemiluminescence signal was imaged using an Odyssey XF Imager (LI-COR ...
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bioRxiv - Neuroscience 2023Quote: ... Digestion reaction for proteins was initially set up with 1 μg of MS grade LysC (Promega, Cat# PI90307) for 3 hours at RT and then continued overnight with addition of 2 μg of trypsin (Promega ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested off the beads by adding 6 μL of 1 μg/μL sequencing grade Trypsin (Promega), diluted in 50 mM acetic acid ...
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bioRxiv - Cell Biology 2023Quote: ... the gel plugs were digested by using 1:50 (Trypsin: Protein amount ratio) Sequencing Grade Modified Trypsin (Promega) at 37 °C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were directly digested by incubation with sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin) overnight at 37°C ...
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bioRxiv - Systems Biology 2023Quote: ... Samples were then digested by a 1:50 (trypsin to protein) ratio of sequencing grade modified trypsin (Promega) for 16 hours at 600 rpm and 25°C ...
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bioRxiv - Plant Biology 2023Quote: ... 1 μg protein was used for dual luciferase transactivation assay using Dual-Luciferase® Reporter Assay System (Promega) following manufacturer’s protocol ...
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bioRxiv - Plant Biology 2019Quote: ... An aliquot containing 4 μg RNA was treated for 45 min with 2 μl of DNase RQ1 (1 μg/μl) at 37 °C (Promega, UK), and purified using an RNAeasy spin column (Qiagen ...
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bioRxiv - Cell Biology 2019Quote: ... cat # 129-02541) overnight at 37°C and then diluted 1/2 and digested with 0.9 µg of trypsin (Promega, cat # V5113) for eight hours at 37°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and then diluted 2-fold with 200 mM ammonium bicarbonate for trypsin digestion (1:10 w:w, 37°C, 8h, Promega cat # V5113).
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bioRxiv - Immunology 2021Quote: ... 2.5 μl TDE1 and 22 μl nuclease-free water were combined and placed at 37°C for 3 min before 0.5 μl of 1% Digitonin (Promega, Cat. #G9441) was added to the master mix ...
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bioRxiv - Cell Biology 2020Quote: ... and then diluted 2-fold with 200 mM ammonium bicarbonate for trypsin digestion (1:10 w:w, 37°C, 8h, Promega cat # V5113). After digestion ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein was digested with Lys-C (Wako) (1:50 enzyme-to-substrate ratio) for 3 h at 25°C and with sequencing-grade modified trypsin (Promega, V5117) at 25°C for 14 h ...
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bioRxiv - Microbiology 2020Quote: ... samples were heated at 80 °C for 15 min and cooled to RT before addition of 1 μg of trypsin (Promega, V511A). Samples were digested at 37 °C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... at an enzyme-to-substrate ratio of 1:100 and subsequently digested overnight at 37°C with sequencing grade trypsin (Trypsin Gold, Mass Spec Grade, Promega, V5280) at an enzyme-to-substrate ratio of 1:50 ...
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bioRxiv - Immunology 2023Quote: ... and then diluted 2-fold with 200 mM ammonium bicarbonate for trypsin digestion (1:10 w:w, 37°C, 8h, Promega cat # V5113). After digestion ...
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bioRxiv - Cell Biology 2023Quote: ... samples were resuspended in 50 µl of 50 mM ABC buffer and digested at 37°C for 16 h using 1 µg of trypsin (Trypsin Gold, Promega, V5280) and 1 µg of LysC (Wako Pure Chemical Corporation ...
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bioRxiv - Cell Biology 2023Quote: ... 30 min) and diluted to 1M Urea with 200 mM ABC for trypsin digestion (1 μg, 37°C, overnight shaking, Promega, #V5113). On the next day ...
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bioRxiv - Immunology 2023Quote: ... for 90 min at 37°C (enzyme-to-substrate ratio of 1:100) and subsequently digested overnight at 37°C with trypsin (Trypsin Gold, Mass Spec Grade, Promega, V5280) at an enzyme-to-substrate ratio of 1:50 ...
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bioRxiv - Cancer Biology 2023Quote: ... Reactions were incubated for 1 hr at 30 °C and luciferase signal was measured using Dual-Glo Luciferase Assay System (Promega E2920).
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bioRxiv - Systems Biology 2024Quote: ... protein digestion was performed overnight by addition of 50 nL of 0.01 ng nL-1 Lys-C (MS grade, Promega, Madison, USA) and 50 nL of 0.04 ng nL-1 trypsin (Promega ...
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bioRxiv - Bioengineering 2023Quote: ... and loaded on a micro S-trap column (Protifi, NY) and digested according to vendor protocol for 1 hour at 50 C by Trypsin Platinum (Promega, WV). Digested material was then eluted from the column by centrifuge at 1000 rpm for 2 minutes and dried in speedvac (Eppendorf ...
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bioRxiv - Bioengineering 2024Quote: ... were taken for overnight digestion with mass spec grade Trypsin/Lys-C Mix (Promega, Madison, WI, cat. No. V5071, 1:20). Samples were reduced with TCEP (final concentration 5 mM ...
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bioRxiv - Biochemistry 2024Quote: ... when the temperature was dropped to 22°C and isopropyl β-d-1-thiogalactopyranoside (IPTG) and L-rhamnose (Promega, Madison, WI) was added at 0.2 mM and 0.1% (w/v ...
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bioRxiv - Cell Biology 2024Quote: ... images produced from HeLa cells seeded on glass-bottom Mattek dishes (P35GC-1.5-14-C) and transfected with TFG-mEGFP-FLAG at 1 µg/µL using Fugene (Promega, cat # PRE2311) for 24 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... the samples were incubated at either 15°C or 37°C for 10 min in Buffer I containing 0.4 U/µL of RNasin (Promega). Following renaturation ...
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bioRxiv - Microbiology 2020Quote: ... and in-solution protease digestion using either a trypsin/Lys-C mix or Glu-C (Promega, Madison, WI, USA). Following RapiGest hydrolysis and desalting ...
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bioRxiv - Biochemistry 2021Quote: ... Proteolytic digestion was performed overnight at 37°C using 0.5 μg Trypsin/Lys-C Mix Mass Spectrometry Grade for each sample (Promega Corporation ...
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bioRxiv - Molecular Biology 2019Quote: PERP protein-protein interactions were isolated using the HaloTag Mammalian Pull-Down System (Promega) and standard protocol ...
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bioRxiv - Plant Biology 2023Quote: ... and the total protein contents were quantified using BCA Protein Assay Reagent (Promega Corporation). The supernatants were stored at –80 ℃ until subsequent analysis.
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bioRxiv - Plant Biology 2024Quote: ... Proteins were expressed with TNT SP6 High-Yield Wheat Germ Protein Expression System (Promega) and reactions were incubated for 12h at 25°C with 10µg pIX-HALO expression clone ...
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bioRxiv - Genetics 2019Quote: ... Purified recombinant luciferase protein (Promega) was used to generate a standard curve.
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bioRxiv - Genomics 2022Quote: ... and the LgBiT Protein (Promega) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Magne Protein G beads (Promega) were used to purify the antibodies according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... In vitro translated proteins (Promega SP6-TNT Quick rabbit reticulocyte lysate system ...
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bioRxiv - Cancer Biology 2022Quote: ... A BCA protein assay (Promega) was used to determine protein concentration ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... HiBiT control protein (#N301A, Promega) dilutions were prepared in the same medium and added (10 µl/well) ...
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Pushed to the edge: hundreds of Myosin 10s pack into filopodia and could cause traffic jams on actinbioRxiv - Cell Biology 2023Quote: ... Halo Standard Protein (Promega, G4491) samples were prepared for final masses of 1 ng ...
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bioRxiv - Biochemistry 2023Quote: ... with purified LgBiT Protein (Promega). Briefly ...
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bioRxiv - Biophysics 2023Quote: ... Protein samples were trypsinized (Promega) in 40mM ammonium bicarbonate (FISHER SCIENTIFIC) ...
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bioRxiv - Cell Biology 2019Quote: ... the protein sample was digested by incubation with sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin) overnight at 37°C ...
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bioRxiv - Systems Biology 2023Quote: ... Samples were diluted 1:3 with 50 mM HEPES pH 8.5 and then digested by a 1:50 (trypsin to protein) ratio of sequencing grade modified trypsin (Promega) for 16 hours at 600 rpm and 25°C ...