Labshake search
Citations for Promega :
6451 - 6500 of 8253 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... Radiolabeled rat ornithine transcarbamylase (OTC) precursor protein was synthesized from plasmid OTC with the TNT Quick Coupled Transcription/Translation kit (Promega #L2080). Upon import into the mitochondrial matrix ...
-
bioRxiv - Developmental Biology 2024Quote: ... Tail clips were taken from each pup on the weaning day and genotyping was performed using the GoTaq® Flexi DNA Polymerase kit (Promega). Separate PCR reactions were set up to amplify either the wildtype or mutant allele using forward wildtype primer (5’-CCCATTGTCAGCGGGCTGTAG-3’) ...
-
bioRxiv - Biophysics 2021Quote: ... cells were transfected with pcDNA3-Kv7.2 WT or mutant (0.5 μg) using FuGENE6 transfection reagent (Promega). At 24 hrs post-transfection ...
-
bioRxiv - Biophysics 2021Quote: ... cells were transfected with pcDNA3-Kv7.2 WT or mutant (0.8 μg) using FuGENE6 transfection reagent (Promega). At 24 hr post-transfection ...
-
bioRxiv - Biophysics 2020Quote: CosM6 cells were transfected with ∼1 µg of wild type or mutant constructs using FuGENE6 (Promega) and were patched within 1-2 days after transfection ...
-
bioRxiv - Cancer Biology 2021Quote: ... the cells were harvested and assayed for luciferase (Luc Assay System with Reporter Lysis Buffer, Promega) in a Beckman Coulter LD400 plate reader.
-
bioRxiv - Cancer Biology 2021Quote: Viability assays were performed following supplier’s instructions from the CellTiter-Glo 2.0 Cell Viability Assay (Promega). Luminescence reading was performed using GloMax® Discover Microplate Reader (Promega).
-
bioRxiv - Cell Biology 2020Quote: ... cells were transfected with pAV-Tornado-RhoBAST plasmid (~200 ng/well) using FuGeneHD transfection reagent (Promega) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2021Quote: ... Cells were incubated for 10 minutes at room temperature with 0.35 µg of pRL-TK (Promega) and either 10 µg of each luciferase reporter vector or 5 µg of each reporter and 5 µg of an additional expression vector ...
-
bioRxiv - Genetics 2021Quote: ... HEK293T cells were harvested for luciferase activity assessment using a dual-luciferase reporter assay system (Promega). The results were normalized to Renilla luciferase activity.
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were harvested 24 h later and assayed with the Dual-Glo Luciferase Assay System (Promega). Firefly luciferase activity was normalized to Renilla luciferase as an internal control for transfection efficiency ...
-
bioRxiv - Molecular Biology 2021Quote: ... cells were lysed and luciferase activity determined with the Dual-Glo luciferase assay system (Promega, UK), following the manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2020Quote: Relative viability was determined in 96 well plates using the CellTiter-Blue Cell Viability Assay (Promega), including a minimum of triplicate wells per sample ...
-
bioRxiv - Molecular Biology 2021Quote: ... The cells were incubated for 24 hours post-transfection before extraction with passive lysis buffer (Promega). Activity levels were measured for Firefly and Renilla luciferase using the Dual-Luciferase Reporter Assay System (Promega ...
-
bioRxiv - Molecular Biology 2021Quote: ... HEK293T cells were transfected with reporter plasmid and various Nsp1 constructs using FuGENE Transfection Reagent (Promega). Luciferase assays were performed using the Dual Luciferase Assay System (Promega) ...
-
bioRxiv - Developmental Biology 2021Quote: ... cells were harvested for luciferase and renilla quantification using the Dual-Luciferase Reporter Assay System (Promega). Luciferase activity divided by renilla activity was used to calculate TOPFlash activity ...
-
bioRxiv - Genomics 2020Quote: ... Cells were collected 48 hours post transfection and assayed using the Dual-Luciferase Reporter system (Promega). Firefly activity was normalized to the Renilla activity and expressed as fold change compared to the luciferase activity of the empty vector (RLU) ...
-
bioRxiv - Microbiology 2022Quote: ... cells were washed in PBS and harvested in 100 μL of 1X passive lysis buffer (Promega). The Dual-Luciferase Assay Reporter Kit (Promega ...
-
bioRxiv - Molecular Biology 2020Quote: ... the cells were washed with PBS and lysed with 100 μL of passive lysis buffer (Promega) and luciferase activity was measured using Bright-Glo Luciferase Assay reagents (Promega ...
-
bioRxiv - Cell Biology 2022Quote: ... HeLa cells were cultured in DMEM+10% FBS+Penicillin/Streptomycin and transfected using FuGENE 6 (Promega). Co-recruitment assays were performed 24 hours after transfection ...
-
bioRxiv - Cell Biology 2022Quote: ... The retroviral or lentiviral constructs were first introduced into packaging cells by FuGENE6 (Cat#E2691, Promega) transfection according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2020Quote: ... 300ng of plasmid DNA for each biological replicate was transfected into HepG2 cells using FuGENE (Promega) in triplicate ...
-
bioRxiv - Genomics 2020Quote: ... Cells were lysed and luciferase substrates provided using the Dual-Glo Luciferase Assay System (Promega E2920). Renilla and firefly luciferase activity were measured using the LUMIstar Omega luminometer ...
-
bioRxiv - Immunology 2021Quote: ... 25 μl of 7.5×104 Jurkat effector cells expressing either human FcγRIIIa or murine FcγRIV (Promega) resuspended in assay buffer were added to each well ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with plasmids encoding fluorescently tagged proteins using FuGENE® HD Transfection Reagent (Promega), following manufacturer’s protocol and assayed 1-3 days after transfection ...
-
bioRxiv - Biochemistry 2020Quote: ... bacmid DNA containing all four subunits was transfected into Sf9 cells using FuGENE® HD (Promega) per manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: All VLPs were produced by transient transfection of HEK293T cells with Fugene 6 (Promega, ref E2691). HEK293T were seeded in 15-cm dishes to reach 60-70% confluency the next day and VLPs were produced by co-transfecting plasmids encoding Gag-eGFP and the VSV-G envelope (pGag-EGFP and pCMV-VSV-G ...
-
bioRxiv - Microbiology 2020Quote: ... HuH7 cell viability and parasite load were determined 44 hours post infection using CellTiter-Fluor (Promega) and Bright-Glo (Promega ...
-
bioRxiv - Microbiology 2021Quote: ATP measurements were performed using the CellTiter-Glo Luminescent Cell Viability Assay (Promega, Madison, Wisconsin USA) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were co-transfected with 5’ SORL1 proximal promoter fragments in pGL4.10[luc2] vector (Promega, E6651) and pRL-TK Renilla control reporter vector (Promega ...
-
bioRxiv - Bioengineering 2021Quote: A mNeonGreen construct was transfected into HEK293T-X cells with FuGENE HD Transfection Reagent (E2311; Promega). The cells were then selected with puromycin (NC9138068 ...
-
bioRxiv - Biochemistry 2021Quote: ... Media were recovered while cells were rinsed in PBS and lysed in Passive Lysis Buffer (Promega). Media was directly subjected to Western Blotting ...
-
bioRxiv - Biochemistry 2020Quote: ... cell metabolic activity or caspase 3/7 activity was measured using the CellTiter-Glo assay (Promega) and Caspase-Glo 3/7 assay (Promega) ...
-
bioRxiv - Neuroscience 2020Quote: ... cells were rinsed with PBS and incubated with the reporter lysis buffer (Promega, Madison, WI, USA) for 15min at rt ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... and luciferase activity was measured in cell lysates (Renilla luciferase assay system, Promega Madison WI, USA).
-
bioRxiv - Pharmacology and Toxicology 2021Quote: 100000 cells or 200 mg tissue were lysed in 100-500 μl Passive Lysis Buffer (Promega) for 15 min at room temperature on an orbital shaker ...
-
bioRxiv - Cancer Biology 2020Quote: ... 20 μL CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS; Promega, Madison, WI, USA) was added and incubated 1−4 hours at 37 °C ...
-
bioRxiv - Cancer Biology 2020Quote: ... Drugs were added 24 hours after seeding and viability was measured using Cell-Titer Glo (Promega) 48 hours after the addition of drugs ...
-
bioRxiv - Cancer Biology 2020Quote: ... transfected cells were analyzed for reporter activity using the Nano-Glo Dual Luciferase Assay System (Promega) as described above.
-
bioRxiv - Cancer Biology 2020Quote: ... transfected cells were analyzed for reporter activity using the Nano-Glo Dual Luciferase Assay System (Promega) as described above.
-
bioRxiv - Plant Biology 2021Quote: Total RNA extraction was performed with the ReliaPrep™ RNA Cell and Tissue Miniprep System (Promega), and cDNA synthesis was conducted with SuperScript™ IV Reverse Transcriptase (Invitrogen) ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cell viability was estimated by measuring the ATP levels using CellTiter-Glo (Promega, Madison, WI, USA).
-
bioRxiv - Microbiology 2020Quote: ... cells were washes twice and followed by the addition of 50 μL Nano luciferase substrate (Promega). After 5 minutes of incubation at room temperature ...
-
bioRxiv - Microbiology 2020Quote: ... cells were washed once with PBS and lysed in 40μl of 1 x CCLR buffer (Promega) for 10 min on a rocking plate at RT ...
-
bioRxiv - Neuroscience 2020Quote: ... the cells were washed with 1X PBS and then lysed with 1X Passive Lysis Buffer (Promega) for 10 mins at 4°C ...
-
bioRxiv - Microbiology 2020Quote: ... and the cells were lysed by the addition of 30 μl of passive lysis buffer (Promega) followed by three freeze-thaw cycles ...
-
bioRxiv - Microbiology 2021Quote: ... and the cells were lysed by the addition of 30 μl of passive lysis buffer (Promega) and one freeze-thaw cycle ...
-
bioRxiv - Microbiology 2021Quote: ... The cytotoxicity of EF was tested in parallel on uninfected cells using the MTS assay (Promega).
-
bioRxiv - Microbiology 2021Quote: ... The infected cells were collected and lysed with 100 μl of 1× passive lysis buffer (Promega). The samples were sonicated for 30 seconds before centrifugation and 5 μl of the supernatants were collected for luciferase expression reading by the dual-luciferase reporter assay system (Promega ...
-
bioRxiv - Microbiology 2021Quote: ... Firefly luciferase assays were performed on 20 μL of cell lysates using Luciferase Assay System (Promega) according to the manufactures instructions ...