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Citations for Promega :
6401 - 6450 of 8253 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 3 ml overnight cultures were subjected to DNA extraction using an automated Maxwell® 16 Tissue DNA purification kit and following the manufacturer’s instructions (Promega, UK). For long-read complete genome analysis ...
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bioRxiv - Biochemistry 2023Quote: ... After 48 h of incubation at 37 °C and 5 % CO2 culture medium was exchanged with fresh growth medium and Luciferase activity was measured with the Dual-Glo® Luciferase Assay Kit (Promega) according to manufactureŕs instructions using the Tecan Spark® plate reader ...
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bioRxiv - Cancer Biology 2023Quote: ... Organoids were cultured for another 5 days before proceeding with microscopy image and viability test by CellTiter-Glo 3D kit (Promega: G9681) following manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... Cells were pretreated with indicated antagonist for 1hr before treatment with 10 nM of PGF2a or 100 nM PGE2 overnight before glucose uptake was measured by Glucose Uptake-Glo™ Assay kit (Promega) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... washed with PBS and inflammasome activation was assessed using qPCR or inflammasome caspase-1 activity was measured using Caspase-Glo® 1 inflammasome assay kit (G9951; Promega) according to manufacturer’s instructions.
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bioRxiv - Biophysics 2023Quote: ... Subsequently we ran the digested fragment on a 1% TAE agarose gel and the desired ∼14 kbp DNA fragment was isolated from an agarose gel using a gel purification kit (Promega, A9282).
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bioRxiv - Immunology 2023Quote: Culture supernatants from the different BMDMs samples were analyzed with the CytoTox 96® Non-Radioactive Cytotoxicity Assay Kit (Promega# G1780). Total cell lysate of non-treated (i.e. ...
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bioRxiv - Genomics 2023Quote: ... DNA desulphonation and cleanup steps were immediately performed following the manufacturer instructions of the MethylEdge® Bisulfite Conversion System kit from Promega. After the column cleanup ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultured in a 6-well plate and transfected with 10 µg of linearised plasmid expressing the SV40 Large T antigen using the FuGene Transfection Kit (Promega, #E2691) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... prior to firefly and Renilla luciferase activity measurements which were performed in triplicate using the Dual-GLO kit (Promega cat. #E2920). The whole procedure was repeated three times independently for each construct.
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bioRxiv - Developmental Biology 2023Quote: Notch reporter 4xcsl::H2B-mvenus plasmid(Nowotschin et al., 2013) was injected at 30ng/µl after purification using the PureYield Midiprep kit (Promega, #A2492). For antisense in situ hybridization probes ...
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bioRxiv - Immunology 2023Quote: Nanoluciferase readings from fecal samples were performed as a proxy for parasite burdens as previously described using the NanoGlo Luciferase reporter assay (NanoGlo Luciferase kit, Promega, N1150) (Vinayak et al. ...
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bioRxiv - Genomics 2023Quote: ... leaves and fruits were collected and in the field and total RNA was extracted with Maxwell® RSC Plant RNA Kit (Promega). Two pools were generated and used for PacBio Iso-Seq library preparation (Procedure & Checklist – Iso-Seq™ Express Template Preparation for Sequel® and Sequel II Systems ...
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bioRxiv - Developmental Biology 2023Quote: ... Targeted sequence of each single clone was validated by Sanger sequencing after gDNA isolation through Wizard® Genomic DNA Purification Kit (#A1120, Promega). For Prkaa1 knockout ...
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bioRxiv - Cancer Biology 2023Quote: ... Two replicate electroporations were each divided into three wells for 48h of culture and readout using the Dual-Glo Luciferase kit (Promega; E2940) in a Bio-Tek Synergy HT Microplate Reader.
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bioRxiv - Plant Biology 2023Quote: ... leaf samples of equal size were collected for protein extractions using the Dual-Luciferase Reporter Assay System kit (Promega, Fitchburg, USA). Glomax 96 microplate luminometer (Promega ...
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bioRxiv - Neuroscience 2023Quote: ... equal amount of RNA (2 μg) from each sample was subjected to cDNA synthesis using reverse transcriptase Kit (Promega Corporation, USA) following standard procedures ...
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bioRxiv - Biophysics 2023Quote: ... from the access handles by running this on a 1% agarose gel and gel purify the right DNA fragment with a gel purification kit (Promega, A9282).
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bioRxiv - Genomics 2023Quote: ... Resulting genomic DNA was assessed for concentration and purity using Promega DNA quantification kit on the Promega Quantus Fluorometer (Promega, Germany) and measurement of A260/A280 ratio on the MultiSkan Sky High spectrophotometer (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: Production of hydrogen peroxide (H2O2) in WT and Alas1 KO brown adipocytes was measured using the ROS-GloTM H2O2 assay kit (Promega #G8820) as per manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... NAD+ and NADH were measured using a protocol adapted from the manual of the NAD+/NADH-Glo Assay Kit (Promega, G9072), as previously described (Li et al. ...
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bioRxiv - Genomics 2023Quote: ... The lysates were generated by adding 100 µL 1X Passive Lysis Buffer included in the Dual-luciferases assay kit (Promega E1960) to the protoplasts and vigorously shaking at room temperature for 15 minutes ...
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bioRxiv - Genomics 2023Quote: ... cells were washed once with cold 1× PBS and the luciferase activities were measured with Perkin Elmer Envision using Promega Dual Luciferase Assay kit (Promega, E1960). The firefly luciferase activity was normalized to renilla luciferase activity for each well ...
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bioRxiv - Microbiology 2023Quote: ... Nucleic Acid Purification with Proteinase K digestion was performed using Maxwell ® RSC Total Viral Nucleic Acid Purification Kit according to the manufacturer’s protocols (Promega, USA). For preparation of bacterial genomic DNA ...
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Sphingosine induction of the Pseudomonas aeruginosa hemolytic phospholipase C/sphingomyelinase, PlcHbioRxiv - Microbiology 2023Quote: We observed induction of the chromosomal plcH-nLuc-plcR reporter in nanoluciferase reporter assays using the Nano-Glo® Luciferase Assay kit (Promega). The assay was conducted as described by the manufacturer with some modifications reported here ...
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bioRxiv - Cancer Biology 2023Quote: ... cytotoxicity and apoptosis were measured 48h and 7 days after the knock-down using ApoTox-Glo triplex assay kit (Promega,G6320) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: Constitutional genomic DNA is extracted from total bone marrow at the remission and purified using magnetic beads (Maxwell® RSC Whole Blood DNA Kit, PROMEGA). Tumor genomic DNA is extracted and purified using magnetic beads (Maxwell® RSC Tissue DNA Kit ...
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bioRxiv - Microbiology 2023Quote: ... The amplified PCR fragments were checked for uniformity and size using 0.8% agarose gel electrophoresis and purified using a PCR fragment recovery kit (Promega, WI, USA). The recovered DNA fragments were sequenced using the MiSeq 500PE v.2 kit (Illumina ...
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bioRxiv - Synthetic Biology 2024Quote: ... Luciferase activity was assayed in an Omega luminescence plate-reader (Fluostar) with four different gains following the instructions of the Nano Dual-Luciferase® Reporter kit (Promega). A further correction for luminescence bleed-through and background signal was applied using the software developed by Mauriet al.58 Then NLuc signal was divided by the FLuc signal to normalize for the transformation efficiency.
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bioRxiv - Cancer Biology 2024Quote: ... The BSP amplification product was purified by Vazyme Biotech (China) amplification product purification kit and cloned into pGEM-Tasy-vector system (Promega, USA). and the percentages of methylated cytosines over total cytosines were calculated.
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bioRxiv - Microbiology 2024Quote: ... Cells were processed at different times post inoculation (pi) according to manufacturer’s instructions (Caspase-Glo® 3/7 Assay kit, Promega, USA) to determine caspase 3/7 activity ...
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bioRxiv - Microbiology 2024Quote: ... and tryptone (Becton Dickinson DifcoTM,)) overnight at 35°C and genomic DNA was extracted using the Wizard® Genomic DNA purification kit (Promega) as previously described [35] ...
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bioRxiv - Microbiology 2024Quote: DNA extraction of the bacterial strains isolated from oysters and cultivated on agar plates was carried with the Wizard® Genomic DNA Purification Kit (Promega) according to the manufacturer instructions ...
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bioRxiv - Physiology 2024Quote: Total RNA was isolated from snap frozen LV wall or kidney after mechanical grinding (Precellys Evolution, Bertin Technologies, France) using the Maxwell RSC microRNA Tissue kit (Promega, USA). RNA was quantified via NanoDrop (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Total RNA was extracted from 10 ⨯ 10 µm FFPE tissue sections using the Maxwell RSC FFPE RNA kit in accordance with the manufacturer’s manual (Promega, United States). RNA concentration was determined using a Qubit 3.0 Fluorometer using the Qubit RNA BR Assay Kit (Thermo Fischer Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: ... Larvae from progenies with low homing rates of dsxFGFP-null were pooled to perform genomic DNA extraction (Wizard® Genomic DNA Purification Kit, Promega) and subsequently do amplicon sequencing on the dsx target to check for the presence of mutations that could have prevented the homing ...
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bioRxiv - Microbiology 2024Quote: ... PCR amplified plasmids and DNA fragments were then purified by the Wizard® SV Gel and PCR Clean-Up kit (Promega) followed by DpnI digestion of purified plasmids ...
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bioRxiv - Microbiology 2024Quote: The high molecular weight genomic DNA of APEC isolates was extracted and purified using a DNA Wizard® genomic purification kit (Promega), according to the instructions from the manufacturer ...
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bioRxiv - Genetics 2024Quote: ... then genomic DNA was extracted with the QIAamp 96 DNA QIAcube HT Kit and quantified using the QuantiFluor® dsDNA System (Promega). 2-5 ng of DNA from every colony was used as template for targeted amplicon sequencing (Fw primer and Rv primer ...
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bioRxiv - Plant Biology 2024Quote: RNA was isolated from plant tissues using a Maxwell RSC Instrument and Maxwell RSC Plant RNA Kit (Promega, Madison, WI, USA) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... ATRX 5ʹ-TGAAACTTCATTTTCAACCAAATGCTC-3ʹ and 5ʹ-ATCAAGGGGATGGCAGCAG-3ʹ All PCR reactions were performed using GoTaq® G2 DNA polymerase kit from Promega following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... cell pellets were washed with 1× PBS and resuspended in either RNA lysis buffer (mirVana kit) containing 0.5 U/μL RNAsin Plus (Promega; Madison, WI) for RNA isolation or 1X radioimmunoprecipitation assay (RIPA ...
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bioRxiv - Molecular Biology 2024Quote: ... Levels of extracellular ATP were measured every 10 mins for 4 hours by using a luciferase activity assay kit (RealTime-Glo™ Extracellular ATP Assay, Promega) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted from 10 whole fly bodies collected on dry ice between ZT 6 and 8 using SV RNA Total Isolation kit (Promega #Z3101) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Each pool was normalized using the ‘Just-a-Plate’ kit (Charm Biotech) then DNA was quantified using the Quantus™ fluorometer (Promega). The final pools were combined in equal molarity and sequenced on the Illumina platform Novaseq 6000 at 250 bp PE.
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bioRxiv - Immunology 2024Quote: LDH levels in tissue culture supernatants were measured by colorimetric formazan product formation using the CytoTox 96® Non-Radioactive Cytotoxicity Assay kit (Promega) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: The insulin secreted from islets ex vivo and plasma insulin from blood samples a were measured using Lumit Insulin Immunoassay kit (Promega, CS3037A05) as per the manufacturer’s instructions and measured using a Flexstation-3.
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bioRxiv - Cancer Biology 2024Quote: JMJD6 and YBX1 constructs containing an upstream T7 promoter were linearized and used as a template for coupled transcription and translation in rabbit reticulocyte lysates according to manufacturer’s protocols (TnT kit,Promega Corporation, USA). Synthesis was confirmed using Western blot analysis using respective antibody ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 µg of total RNA was reversed-transcribed with High-Capacity cDNA Reverse Transcription Kit (Thermo-Fisher # 4368814) and qPCR performed with GoTaq (Promega # M3001) according to instructions with primer sets listed in Table S5 and analyzed likewise 2,−ΔΔCt method (Livak & Schmittgen ...
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bioRxiv - Cell Biology 2024Quote: ... All the samples were collected and stored at -20 °C for assay of glucagon with either Lumit Glucagon kit (Promega; W8020) or glucagon ELISA kit (MSD ...