Labshake search
Citations for Promega :
601 - 636 of 636 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2024Quote: The viability of human fibroblasts embedded in the bioprinted gel constructs was analyzed by monitoring the metabolic activity through the fluorimetric resazurin reduction method (CellTiter-Blue, Promega, G8080) at 1 ...
-
bioRxiv - Genetics 2020Quote: ... The appropriate band (1595 bp) was excised and purified as recommended by the Wizard SV Gel and PCR Clean-Up System (Promega, Fitchburg, USA). The blunt end PCR product was A-tailed with a 20 min incubation step at 72 °C by adding 0.2 mM dATP ...
-
bioRxiv - Molecular Biology 2020Quote: ... The protein was reduced by 10mM DTT in 100mM Ambic for 30 min and alkylated with 55mM iodoacetamide in 100mM Ambic for 20 min followed by in-gel digestion with 0.3 mg chymotrypsin (modified, Promega, Madison, WI, USA) in 50 mM ammonium bicarbonate for 5 h at 40°C ...
-
bioRxiv - Genetics 2019Quote: ... The longer band containing exon14b was then excised from the gel and purified using Zymoclean gel DNA recovery kit (Zymoresearch, Irvine, CA) and cloned using pGEM-T Easy Vector System (Promega, Madison, WI). The plasmid was used in a transformation into Subcloning Efficiency DH5α Competent Cells (Invitrogen ...
-
bioRxiv - Biophysics 2022Quote: ... reduced with 5 mM of DTT and alkylated with 11 mM iodoacetamide which was followed by in-gel digestion with trypsin (Promega, Madison, WI), chymotrypsin (Promega ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Amplified DNA products were purified using the Wizard® SV Gel and PCR Clean-Up System (Promega GmbH, Germany, Art. No. A9282). In all agarose gels ...
-
bioRxiv - Molecular Biology 2020Quote: ... The band was cut and washed before the in-gel digestion of the proteins overnight at 37°C with a solution of modified trypsin (sequence grade, Promega, Charbonnières, France). The resulting peptides were extracted from the gel by one round of incubation (15 min ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/µl modified sequencing-grade trypsin (Promega, 182 Madison, WI) at 4ºC ...
-
bioRxiv - Microbiology 2020Quote: ... All PCR products were purified with an AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, USA) and quantified using a QuantiFluor™-ST (Promega, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: ... all RT mixture of samples were pooled and purified with Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA) according to the manufacture’s manual except for using 80% EtOH instead of Membrane Wash Solution ...
-
bioRxiv - Genetics 2019Quote: The target genomic region was amplified using PCR and purified using Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI). The DNA sequence of the PCR fragment was determined using cycle sequencing with BigDye v3.1 reagents (Applied Biosystems ...
-
bioRxiv - Developmental Biology 2022Quote: ... protein in the gel pieces was digested using 20 ng/μl of Trypsin solution made in 25mM ammonium bicarbonate (Promega, Madison, WI) by incubating at 37°C overnight ...
-
bioRxiv - Physiology 2022Quote: ... agarose gel and the appropriate bands were cut out and purified according to the Wizard SV Gel and PCR Clean-Up System (Promega, Fitchburg, USA). A 20 min A-tailing step (72 °C ...
-
bioRxiv - Microbiology 2023Quote: ... The 650 bp positive fragment was purified by Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, Madison, WI, USA).
-
bioRxiv - Microbiology 2023Quote: ... The 1800 bp positive amplicons was purified using Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, Madison, WI, USA).
-
bioRxiv - Molecular Biology 2022Quote: ... and purified using the Wizard SV Gel and PCR Clean-R19 purification systems following the instructions by the manufacturer (Promega, Madison, USA). The positive PCR amplicons were bidirectionally sequenced and gathered using BioEdit software (Hall ...
-
bioRxiv - Plant Biology 2023Quote: ... The 16S rDNA PCR-amplified fragments were purified by Wizard® SV Gel and PCR Clean-Up System (Promega, New Delhi, India) and digested by HaeIII (SRL ...
-
bioRxiv - Physiology 2022Quote: Capped cRNAs of the two Atlantic salmon PepT2 were synthesized by in vitro transcription using T7 RNA polymerase from cDNAs in pSPORT1 linearized with NotI and purified with Wizard SV Gel and PCR clean-up system (Promega Italia, Milan, Italy). The purified cRNAs were quantified by NanoDropTM 2000 Spectrophotomer (Thermo Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Gel pieces were resuspended in 20 μL l of trypsin solution (25 ng/ μL trypsin in 100 mM NH4HCO3) (Promega, Cat. No V5111) and incubated at 4°C for 20 min ...
-
bioRxiv - Genomics 2019Quote: ... amplicons from each replicate were pooled and purified with the Wizard SV Gel and PCR Cleanup System kit (Promega Corporation, Madison, Wisconsin, USA).
-
bioRxiv - Microbiology 2019Quote: ... Supplementary Table 4) and DNA sequencing of the products following clean-up (Wizard SV Gel and PCR Clean-Up System, Promega, Southampton, Hampshire, UK) using dye terminator chemistry (Eurofins ...
-
bioRxiv - Microbiology 2021Quote: ... knowlesi elongation factor-1 alpha (pkef1-alpha) 5’ UTR were isolated from agarose gels and cloned into the pGEM-T vector (Promega, Madison, WI, USA). To replace the pfcam 5’ UTR sequence that drives luciferase transcription in the pD-pfcam-Luc ...
-
bioRxiv - Biochemistry 2021Quote: ... Amplified PCR products were visualized by blue light illumination and the 504/505 bp bands were excised and the amplified PCR product purified using the Wizard SV Gel and PCR Clean-Up kit (Promega, Madison, WI, USA). Extracted DNA was Sanger sequenced at the University of Michigan Advanced Genomics Core and analyzed for presence of the single base pair deletion using DNAStar LaserGene SeqMan Pro 15 ...
-
bioRxiv - Developmental Biology 2020Quote: ... All RACE-PCR products were purified using GeneJET Gel Purification Kit (Fermentas, Hanover, MD, USA) and ligated into pGEM-T vector (Promega, Madison, WI, USA) and transformed into competent JM-109 Escherichia coli cells (Promega ...
-
Highly Versatile, Non-Invasive Method for Collecting Buccal DNA from Free-Ranging Non-Human PrimatesbioRxiv - Genetics 2021Quote: ... 750 µL of each supernatant was processed using a commercially available DNA clean-up system (Wizard SV Gel and PCR Clean-Up System; Promega, Madison, WI, USA), and the DNA was finally eluted with 50 µL pure water ...
-
bioRxiv - Molecular Biology 2022Quote: For high-resolution DNA electrophoresis resolving the nucleosome repeats genomic DNA was purified from chromatin samples using the Wizard SV Gel and PCR Clean-Up System (Promega, catalog no. A9281) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... PCR products were purified with the Nucleospin gel and PCR clean-up kit (Macherey Nagel) and transcribed in vitro using T7 RNA polymerase (RiboMAX system; Promega, Madison, WI, USA). RNA was purified from the sample using the Nucleospin RNA clean-up kit (Macherey Nagel) ...
-
bioRxiv - Molecular Biology 2023Quote: The DNA was extracted using the Promega PCR Cleaning Kit (Wizard® SV Gel and PCR Clean-Up System, Promega Cat.-No. A9285) as indicated in the manual ...
-
bioRxiv - Immunology 2024Quote: ... 5-GGATCCACACGGTGCAAAGAGAGACCC-3’ and 5′-TCGGCCTTTCAGACTAATCTTATCAGC-3’ The PCR products were gel purified and cloned into the pGEM-T vector (Promega; Madison, WI, USA). The inserted PCR fragments of individual clones were sequenced by Tsing KE Biological Technology ...
-
bioRxiv - Microbiology 2022Quote: ... The product of the ITS gene amplification was cleaned and purified using the Wizard□SV Gel and PCR Clean-Up System (Promega, Madison, Wl, United States), following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2022Quote: ... then purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) and quantified using a Quantus™ fluorometer (Promega, Madison, WI, USA).
-
bioRxiv - Cell Biology 2019Quote: ... then alkylation solution was removed and gel pieces were hydrated for 45 min at 4 °C in digestion solution (5 ng/μl trypsin, sequencing grade, Promega, in 25 mM AB). The trypsin digestion proceeded for 2 hours at 37 °C on Thermomixer (750 rpm ...
-
bioRxiv - Cell Biology 2019Quote: ... then alkylation solution was removed and gel pieces were hydrated for 45 min at 4°C in digestion solution (5 ng/μl trypsin, sequencing grade, Promega, in 25 mM AB). The trypsin digestion proceeded for 2 hours at 37°C on Thermomixer (750 rpm ...
-
bioRxiv - Plant Biology 2021Quote: ... AccuPrep® Plasmid Mini Extraction Kit (Bioneer Corporation, Republic of Korea) and Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, USA) were used for plasmid and PCR product extraction and purification ...
-
bioRxiv - Plant Biology 2023Quote: ... The PHN-SpCas9-4×BsaI was then digested with BsaI and purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA). The annealed oligos FaPDSF1/R1 and FaPDSF2/R2 were mixed and ligated to the purified pHN-SpCas9-4×BsaI to form the final pHN-SpCAS9-FaPDSsgRNAs construct.
-
bioRxiv - Neuroscience 2023Quote: ... before the bands corresponding to the homozygous amplicon size were excised and purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, Madison, Wisconsin, USA) according to the manufacturer’s protocol ...