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Citations for Promega :
251 - 300 of 636 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2021Quote: ... PCR products were purified with Wizard SV Gel and PCR Cleanup kit (Promega). Purified products were sequenced at Sequetech DNA Sequence Service (Mountain View ...
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bioRxiv - Cell Biology 2019Quote: ... gel pieces were dried and rehydrated with 12.5ng/mL trypsin (Promega, Madison, WI) solution in 25mM ammonium bicarbonate on ice for 30min ...
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bioRxiv - Genomics 2021Quote: ... The PCR products were purified via gel excision and column purification (Promega, A9282), and then inserted into the BsmBI-digested lentiCRISPR-v2-Blast-Puro vector by Gibson assembly (New England Biolabs ...
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bioRxiv - Physiology 2020Quote: ... PCR amplicons were resolved on a 2% agarose gel (Promega Corporation, Madison, USA) pre-stained with Ethidium bromide (0.5 μg/ml ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product was purified by gel extraction and digested with DpnI (Promega) for 1 h to remove residual template DNA before a second round of purification ...
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bioRxiv - Cell Biology 2019Quote: ... Gel slices were rehydrated with a solution of 12.5 ng/µl trypsin (Promega Trypsin Gold ...
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bioRxiv - Cell Biology 2022Quote: Proteins were digested from gels with 0.02 μg/μL modified porcine trypsin (Promega) after reduction with dithioerythritol and iodoacetamide ...
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bioRxiv - Molecular Biology 2022Quote: Purified EXT1-EXT2 was digested in-gel using trypsin (modified, sequencing purity, Promega), as previously described67 ...
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bioRxiv - Plant Biology 2022Quote: ... The NPR1-YFP band was cut and in-gel digested by trypsin (Promega). The phosphorylated peptides of NPR1 protein were detected by LC-MS/MS at the Mass Spectrometry facility at CAS for Excellence in Molecular Plant Sciences.
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bioRxiv - Immunology 2022Quote: ... and purified with the Wizard SV Gel and PCR Clean-Up System (Promega) after DNA electrophoresis ...
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bioRxiv - Cell Biology 2022Quote: The protein in the gel lanes were digested with 0.1 µg trypsin (Promega) in 20 µl 25 mM ammonium bicarbonate ...
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bioRxiv - Microbiology 2023Quote: ... After purification with the Wizard SV Gel and PCR Clean-Up System (Promega), the template plasmid DNA was digested with DpnI and used in the transformation reaction.
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bioRxiv - Bioengineering 2023Quote: ... The amplicon was purified Wizard SV Gel and PCR Clean-up System (Promega) and was used to produce preselected libraries ...
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bioRxiv - Immunology 2023Quote: ... destained and digested in-gel with 200 ng modified trypsin (sequencing grade, Promega) and Rapigest (TM ...
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bioRxiv - Molecular Biology 2023Quote: ... alkylated with iodoacetamide and in-gel digested with 0.1 µg trypsin (V511, Promega) per slice using a Freedom EVO150 robotic platform (Tecan Traging AG ...
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bioRxiv - Biochemistry 2023Quote: ... and peptides were generated by in-gel digestion using trypsin (sequencing-grade (Promega)) at 37°C overnight ...
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bioRxiv - Plant Biology 2023Quote: ... purified using Wizard® SV Gel and PCR Clean-Up System (Promega, USA), according to the protocol provided by the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were then in-gel proteolyzed using Sequencing grade modified porcine trypsin (Promega), and peptides were eluted (80% acetonitrile ...
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bioRxiv - Biochemistry 2023Quote: ... In-gel digestion was performed with trypsin (Trypsin Gold, Mass Spectrometry Grade, Promega, Madison ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting bands were gel purified and cloned into pGEM T-easy (Promega) and sent out for Sanger sequencing.
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bioRxiv - Plant Biology 2023Quote: ... and purified using Wizard® SV Gel and PCR Clean-Up System (Promega). Next ...
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bioRxiv - Cell Biology 2024Quote: ... gel bands were processed by following a standard protocol 87 with trypsin (Promega) digestion at a 10-ng/μL concentration overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... Amplicons were purified with Wizard® SV Gel and PCR Clean-Up System (Promega) and sequenced via the Eurofins TubeSeq service ...
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bioRxiv - Cell Biology 2020Quote: ... and purified using the Wizard SV Gel and PCR Clean-up System (Promega, A9281) as per manufacturer directions ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR products were purified with the Wizard SV Gel and PCR Cleanup System (Promega). Plasmid DNA was isolated from bacterial cultures with the QIAprep Spin Miniprep Kit (Qiagen) ...
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bioRxiv - Genomics 2019Quote: ... the gel slices were then hydrated with 5 ng/uL sequencing grade trypsin (Promega) in 50 mM ammonium bicarbonate and digested overnight at 37°C on an orbital shaker ...
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bioRxiv - Developmental Biology 2020Quote: ... The gel pieces were hydrated and incubated with 2.5 ng/μl of trypsin (Promega) in 50 mM ammonium bicarbonate (ABC ...
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bioRxiv - Physiology 2020Quote: ... and in-gel tryptic digestion was carried out using ProteaseMAX reagent (Promega, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: ... purified and concentrated using Wizard® SV Gel and PCR Clean-Up System (Promega). Sanger sequencing confirmed correct amplification of PCR fragments ...
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bioRxiv - Immunology 2021Quote: Proteins were in-gel digested with sequencing grade modified trypsin (Promega GmbH, Walldorf, Germany) similar to the procedure described by Pandey et al ...
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bioRxiv - Cancer Biology 2021Quote: ... Each sample was cut into 3 slices and in-gel digested with trypsin (Promega) as previously described15 ...
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bioRxiv - Neuroscience 2021Quote: ... Resulting products were purified using Wizard SV Gel and PCR Clean-Up columns (Promega) and submitted for Sanger and NGS (Amplicon EZ ...
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bioRxiv - Neuroscience 2020Quote: ... Single bands were excised according to their predicted fragment size and gel-purified (Promega Wizard SV Gel and PCR Clean-Up System ...
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bioRxiv - Microbiology 2021Quote: ... Products were cleaned with the Wizard SV Gel and PCR Clean-Up System (Promega) and cloned with the pGEM-T easy Vector Systems (Promega) ...
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bioRxiv - Microbiology 2019Quote: ... which was then purified using the Wizard Gel and PCR Clean-Up System (Promega) and submitted to a third party for sequencing.
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bioRxiv - Physiology 2019Quote: ... Proteins were in-gel digested using 0.6 µg of modified sequencing grade trypsin (Promega) in 50 mM ammonium bicarbonate overnight at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... gel purified-PCR products were ligated into NheI/NcoI-digested pGL3-basic vector (Promega) immediately upstream of the luciferase start codon ...
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bioRxiv - Microbiology 2019Quote: ... we performed Pulse Field Gel Electrophoresis (PFGE) on S1 nuclease (Promega, Madison, WI, USA) digested DNA ...
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bioRxiv - Cell Biology 2021Quote: ... and gel bands were manually excised and digested with sequencing-grade trypsin (Promega, USA). The digested peptides were analyzed using a QExactive mass spectrometer (Thermo Fisher ...
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bioRxiv - Evolutionary Biology 2020Quote: ... in vitro expressed DBDs (see above) and the Gel Shift Assay Core System (Promega). Double-stranded DNA probes were generated by annealing sense and antisense oligonucleotides (see Table S-M1a ...
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bioRxiv - Developmental Biology 2020Quote: ... and 200 ng purified recombinant mouse DLX2 protein in Gel Shift Binding Buffer (Promega). Unlabeled probe was added for ‘cold’ competition ...
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bioRxiv - Developmental Biology 2021Quote: ... The gel-purified fragment was cloned into the p-GEM-T Easy vector (Promega) and digoxigenin-labelled probes generated by in vitro transcription using a DIG RNA labelling kit (Roche).
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CCG•CGG interruptions in high penetrance SCA8 families increase RAN translation and protein toxicitybioRxiv - Genetics 2021Quote: ... PCR bands were extracted using Wizard SV Gel and PCR Clean-Up System (Promega) and ...
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bioRxiv - Evolutionary Biology 2021Quote: ... PCR products were then purified (Promega Wizard SV Gel and PCR Clean-Up System) and sequenced on both strands (Eurofins Genomics ...
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bioRxiv - Microbiology 2020Quote: ... followed by extraction using the Wizard SV gel and PCR clean-up system (Promega). The purified candidate genes were cloned into pCR8/GW/TOPO entry vectors (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... gel pieces were destained and then incubated with 10 ng/μl trypsin (Promega, #V5111) overnight at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: After PCR clean-up using Wizard SV Gel and PCR Clean-Up (Promega; A9281), samples were run in 2% agarose gel to ensure that the PCR product is compose of a single amplicon in the appropriate size ...
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bioRxiv - Microbiology 2021Quote: ... DNA was purified with the Wizard® SV Gel and PCR Clean system (Promega). The DNA concentration of amplicon was measured by NanoDrop (ThermoFisher) ...
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bioRxiv - Cancer Biology 2020Quote: ... in-gel purified and ligated into the pGEM-T Easy vector (Promega, Cat. A1360) and submitted to Sanger sequencing ...
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bioRxiv - Microbiology 2022Quote: ... were purified using Wizard® SV Gel and PCR Clean-Up System (Promega, USA), and then directly sequenced in both directions by Macrogen Inc ...