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Citations for Promega :
601 - 650 of 708 citations for TMB Substrates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... was dispensed into white 96 well luminometer plates and 45 µL of 1 x NanoGlo Lysis Buffer containing 1:1000 NanoGlo substrate (Promega) was injected into wells ...
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bioRxiv - Microbiology 2020Quote: ... and LgBiT Protein (1:100)/HiBiT Lytic Substrate (1:50) in Nano-Glo HiBiT Lytic Buffer (25 μl) (Nano-Glo HiBiT Lytic Detection System; Promega) were mixed and incubated for ten minutes at room temperature according to the modified manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 4 µl of the lysates were mixed with 20 µl of Renilla Luciferase Assay Buffer and 1/200 of substrate from the Renilla Luciferase assay system (Promega) and measured immediately in a luminometer for 2 seconds ...
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bioRxiv - Developmental Biology 2020Quote: ... the cells for the luciferase assay were exposed to 100 μL of a 1:1 dilution of DMEM and luciferase substrate (ONE-Glo Luciferase Assay System, Promega), and luminescence from each well measured in a GloMax-96 Microplate Luminometer (Promega) ...
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bioRxiv - Biochemistry 2020Quote: ... diluted in blocking buffer (1:10,000) was used as a secondary antibody and immunogenic bands were developed by the addition of BCIP/NBT substrate (Promega). The reaction was stopped with distilled water.
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bioRxiv - Immunology 2021Quote: ... 50 μL of the supernatant was removed from treated wells and the luminescence values were again recorded on a plate reader (Mithras LB 940, Berthold Technologies) in the presence of the nano luciferase substrate furimazine (2 μL/mL, Promega).
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bioRxiv - Microbiology 2021Quote: ... 40 µL of medium from selected wells was replaced with Nano-Glo HiBiT extracellular buffer with LgBiT and furimazine substrate (Promega). Readings were taken after a 30 min room temperature incubation as described above.
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bioRxiv - Microbiology 2020Quote: ... The expression of firefly luciferase was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). The percentage of infectivity was calculated as the ratio of luciferase readout in the presence of mAbs normalized to luciferase readout in the absence of mAb ...
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bioRxiv - Neuroscience 2021Quote: ... or methadone were added for another 10 min followed by addition of 20 μL of the RLuc substrate coelenterazine h (Promega), at 5 μM final concentration ...
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bioRxiv - Developmental Biology 2020Quote: ... Larvae were extensively washed in PBST and the in situ hybridization signal was revealed with NBT/BCIP substrate (Promega, USA). Lymph glands were mounted in 70% glycerol ...
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bioRxiv - Microbiology 2020Quote: ... Luciferase activity was then measured using Tristar multiwell luminometer (Berthold Technology, Bad Wildbad, Germany) for 2.5 seconds by adding 20 µl of Nano-Glo® substrate (Promega Inc ...
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bioRxiv - Plant Biology 2020Quote: ... The luciferase assay was performed by thoroughly mixing 10 μl of a sample with 50 μl of the luciferin substrate (Luciferase Assay System, Promega) and measuring the luminescence intensity after 10 and 20 seconds using a luminometer (TD-20/20 Luminometer ...
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bioRxiv - Microbiology 2022Quote: ... Germany) for 2.5 s by adding 20 μL of Nano-Glo® substrate in assay buffer (Promega Inc, WI, USA). Uninfected monolayer of Vero cells treated identically served as controls to determine basal luciferase activity for obtaining normalized relative light units ...
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bioRxiv - Biochemistry 2022Quote: ... 3.5 mM MgCl2, and 2 mM ATP (for nucleic acid substrates containing RNA, 2 U/μl of RNasin Ribonuclease Inhibitor (Promega)) for 30 min at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... were anesthetized with isoflurane and imaged immediately under an in vivo imaging system (IVIS, AMI HTX) after retro-orbitally injected with 100 µl of Nano-Glo luciferase substrate (Promega). The bioluminescence data acquisition and analysis were performed using the Aura program (Spectral Imaging Systems) ...
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bioRxiv - Microbiology 2022Quote: ... Luciferase reporter gene expression was quantified 60-72 h after infection upon lysis and the addition of Bright-GloTM Luciferase substrate (Promega). Data were retrieved from a BioTek microplate reader with Gen 5 software ...
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bioRxiv - Microbiology 2022Quote: ... used as PCR template and directly for luminescence quantification according to the manufacturer’s instructions (20 μL phage plus 20 μL substrate, Nano-Glo® Luciferase Assay System, Promega).
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bioRxiv - Plant Biology 2023Quote: ... Luciferase activity was measured combining by combining 20 μl of sample with 50 μl of enzyme substrate (Luciferase 1000 Assay System, #E4550, Promega), vortexing sample briefly and counting photons for 5 s in the luminometer (Luminoskan TL plus ...
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bioRxiv - Microbiology 2023Quote: ... The plate was incubated for 48 hours at 37°C in a CO2 incubator and luciferase activity was quantified by adding nano-glo luciferase substrate (Promega). The luminescence was recorded by using Cytation 5 plate reader (BioTek inc) ...
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bioRxiv - Microbiology 2022Quote: ... a rabbit polyclonal antibody was used followed by detection with a goat anti-rabbit secondary antibody conjugated to horseradish peroxidase and developed using a chemiluminescent substrate (Promega). Immunoblot detection of 6xHIS and 2A peptide tags were determined through anti-Histidine tag (AD1.1.10 ...
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bioRxiv - Microbiology 2023Quote: ... cell culture supernatants from the same infections were collected and used to measure Nluc expression using the Nano-Glo luciferase substrate (Promega) and the microplate reader (BioTek Synergy) ...
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bioRxiv - Biochemistry 2022Quote: ... Each 70 μl reaction is separated into three individual 20 μl reactions and 100 μl of Renilla Luciferase assay substrate (Promega) is added to each reaction ...
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bioRxiv - Microbiology 2023Quote: ... cells grown on white 96-well plates with clear bottoms were transfected with a polio replicon RNA expressing the Renilla luciferase gene in the complete medium supplemented with 30 μM of cell-permeable Renilla luciferase substrate Enduren (Promega). The plates were sealed with a transparent oversheet and incubated directly in the heated chamber of a multifunctional M3 microplate reader (Molecular Devices) ...
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bioRxiv - Cell Biology 2023Quote: ... nitrocellulose membranes were blocked in 5% skimmed milk incubated with primary and secondary antibodies conjugated with alkaline phosphatase and developed with NBT/BCIP as substrates (Promega).
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Engineering synthetic CopT/A-based genetic circuits for miRNA imaging and functional gene regulationbioRxiv - Synthetic Biology 2023Quote: ... 50 μL of coelenterazine substrate was added to the wells and Gluc activity was measured using a Glomax-20/20 luminometer (Promega).
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bioRxiv - Immunology 2023Quote: ... and luciferase activity was measured in a Tecan infinite 200Pro by automatic injection of 50 μl luciferase assay substrate (Promega) and 10 sec integration time.
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bioRxiv - Molecular Biology 2023Quote: ... Luminescence was measured for each N1-X/N2-Y pairwise combination by auto-injecting 50 µL Renilla luciferase substrate (Renilla Luciferase Assay system, catalog No. E2820, Promega) per well and integrating light output for 4 s using a TriStar luminometer (Berthold) ...
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bioRxiv - Molecular Biology 2023Quote: ... were mixed with 75ul of 0.1% Triton X-100 in PBS and 25 μl of substrate (Bright-Glow™ Luciferase Assay System, Promega), and 50ul aliquots of the mix were distributed in triplicate wells of a 96-wells white plate and analyzed in a microplate luminometer.
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bioRxiv - Bioengineering 2023Quote: ... The day after the plates were pre-cooled (RT, 15’) supplemented with luciferase substrate (Bright Glo Luciferase Assay System, Promega) incubated (RT ...
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bioRxiv - Microbiology 2023Quote: ... and LgBiT Protein (1:100)/HiBiT Lytic Substrate (1:50) in Nano-Glo HiBiT Lytic Buffer (25 μl) (Nano-Glo HiBiT Lytic Detection System; Promega) were mixed and incubated for 10min at room temperature according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: HEK293T cells were transfected with a FLAG-tagged human DHHC20-expressing plasmid and a corresponding HA-tagged substrate-expressing plasmid using FuGENE transfection reagent (Promega) and incubated overnight ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10 μL of stopped reaction samples were mixed with 50 μL of luciferase substrate solution (Luciferase Assay System kit, Promega) in a white flat bottom 96-well microplate ...
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bioRxiv - Microbiology 2023Quote: ... injecting 50 μl per well of coelenterazine substrate (Nanolight Technologies, 2 μg/ml) and analysing luminescence on a FLUOstar OPTIMA luminometer (Promega). Fold inductions were calculated by normalising to a mock-treated control.
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bioRxiv - Immunology 2023Quote: ... on day three the media was completely removed and 60 µL lysis buffer together with 60 µL luciferase substrate (Promega) was added per well ...
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bioRxiv - Immunology 2023Quote: ... prepared by adding NanoDLR™ Stop & Glo® Substrate 1:100 into NanoDLR™ Stop & Glo® Buffer (Promega, USA), were added to the samples ...
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bioRxiv - Neuroscience 2023Quote: ... 1µl lysate was diluted with 39 µl PBS before being mixed with 40 µl of Dual-Glo FLuc substrate (Promega). After 10 min incubation ...
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bioRxiv - Cancer Biology 2023Quote: ... 30 μL of the cell lysate was transferred into a white 96-well plate and 30 μL of firefly luciferase substrate (Promega) was added ...
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bioRxiv - Immunology 2024Quote: ... Cells were then collected and washed before cell lysis and addition of luciferin substrate from the Bright-Glo Luciferase Assay System (Promega). The resulting luminescent signal was measured using a Tecan Infinite M200 Pro ...
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bioRxiv - Microbiology 2024Quote: ... difficile was mixed with 50 µL kit reagents (100-fold diluted LgBiT protein, 50-fold diluted substrate in kit buffer, Promega). Subsequently ...
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bioRxiv - Bioengineering 2023Quote: ... luminescence was read by removing 150 µL of media and adding 50 µL of substrate from the Nano-Glo Luciferase Assay System (N1110, Promega). The mixture was incubated for 3 minutes in a white-walled plate before analysis with a SpectraMax M3 plate reader (Molecular Devices).
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bioRxiv - Microbiology 2024Quote: ... The hydrolase activity of the UDP-substrate was detected as the release of UDP by the UDP-Glo assay (Promega) after 20 min of incubation with UDP-Glo detection reagent ...
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bioRxiv - Bioengineering 2024Quote: ... Bioluminescence measurements were taken by combining 25 µL of the sample solution with an equal volume of prepared buffer-reconstituted nluc substrate as detailed by the manufacturer (NanoGlo Luciferase Assay System; Promega). Measurements were taken every 20 min (225 min total ...
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bioRxiv - Bioengineering 2024Quote: ... the 1X Renilla-luciferase substrate mixed with a Stop & Glo buffer (100 µL/well, Promega Dual Glo kit, cat # E1960) was added ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cells were pre-incubated in Hanks’ balanced salt solution with increasing concentrations of compound for 60 minutes before stimulation with 10 nM CXCL12 and addition of 2 µM Renilla Luciferase substrate coelenterazine-h (Promega). After 20 minutes ...
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bioRxiv - Microbiology 2020Quote: ... At 24 h post-transfection media was removed and Nluc enzymatic activity was measured using a Berthold Centro-XS luminometer by injecting 50 μL of luciferase substrate reagent (#E2820, Promega, France) per well and counting luminescence for 2s ...
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bioRxiv - Molecular Biology 2021Quote: ... 8uL of reaction was mixed with 8uL of Nano-Glo buffer/substrate mix following the Nano-Glo Luciferase Assay System (Promega N1110) instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... the cells were simultaneously lysed and supplemented with a luciferase substrate by addition of equal volume of Steady-Glo reagent (Promega, E2520) directly to the assay media ...
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bioRxiv - Physiology 2019Quote: ... Samples were mixed with the same amount of substrate provided by the One Glo Luciferase Assay System (Promega, Mannheim, Germany, #E6110) immediately before signal detection ...
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bioRxiv - Microbiology 2020Quote: ... The plates were cultured for two days at 37°C with 5% CO2 after which the supernatant was removed and replaced with 50 μl Nano-Glo Luciferase Substrate (Promega, Inc.). Light emission was measured in an Envision 2103 Multi-label plate reader (PerkinElmer ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein was digested with Lys-C (Wako) (1:50 enzyme-to-substrate ratio) for 3 h at 25°C and with sequencing-grade modified trypsin (Promega, V5117) at 25°C for 14 h ...