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Citations for Promega :
451 - 500 of 708 citations for TMB Substrates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The reaction was started by addition of 1:4000 NanoGlo substrate (Promega, catalogue number N1110). The reaction was incubated for 5 minutes and the emission was measured at wavelengths of 445-470 nm and 520-545 nm using Tecan Spark multimode plate reader with luminescence readout and a settle time of 10 ms and integration time of 500 ms ...
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bioRxiv - Biochemistry 2020Quote: ... and mixed with the nanoluciferase substrate Nano-Glo (Promega cat# N1120, final dilution 1:200) for 2 min before measuring luminescence signals in a POLARstar OMEGA plate reader (BMG Labtech ...
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bioRxiv - Plant Biology 2021Quote: ... Protein preparation and luciferase– substrate interaction were conducted with a Luciferase Assay Report Kit (Promega). Luciferase activity was measured by a microplate reader (Thermo Scientific Varioskan Flash) ...
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bioRxiv - Cancer Biology 2020Quote: Furimazine was purchased as NanoLuc substrate in the Nano-Glo® Luciferase Assay System (Promega) and was diluted 50x in media for in vitro experiments and 50x in PBS for in vivo experiments ...
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bioRxiv - Cell Biology 2021Quote: ... trypsin-like and caspase-like activities were measured separately using the relevant substrates from Promega (Suc-LLVY-Glo ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 10% FBS and 1:1000 Nano-Glo® Luciferase Assay Substrate (Furimazine, Promega). First ...
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bioRxiv - Microbiology 2022Quote: ... Firefly luciferase was quantified whereby media was replaced with 50 µL Bright-Glo substrate (Promega) diluted 1:2 with PBS and read on a GloMax Multi+ Detection System (Promega).
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bioRxiv - Microbiology 2022Quote: ... cells were lysed with NanoGlo substrate and lysates were quantified in a GloMax Explorer (Promega) according to manufacturer’s instructions for all competitive inhibition assays.
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bioRxiv - Molecular Biology 2021Quote: ... FLuc activity was measured using a commercial substrate (Beetle-Juice, PJK; Luciferase Assay System, Promega) and Hidex Sense Plate Reader (Hidex).
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bioRxiv - Microbiology 2020Quote: ... followed by measuring luciferase activity (relative light units; RLUs) using Luciferase Substrate Buffer (Promega Inc) on a 2300 EnSpire Multilabel Plate Reader (PerkinElmer ...
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bioRxiv - Immunology 2021Quote: ... Following this incubation luciferase activity was measured in the presence of D-luciferin substrate (Promega) using a Centro LB960 plate luminometer (Berthold) ...
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bioRxiv - Molecular Biology 2020Quote: ... samples were put on ice and mixed with 50 μL 1x Renilla-Glo substrate (Promega) in Renilla-Glo (Promega ...
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bioRxiv - Immunology 2020Quote: ... Luciferase intensity was measured on a luminometer using Bright-Glo luciferase substrate (Promega, PR-E2620) following manufacturer’s directions ...
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bioRxiv - Systems Biology 2019Quote: ... as a substrate and luminescence was measured with the GloMax® 96 Microplate Luminometer (Promega). Luciferase data was repeated at least 3 times with similar patterns ...
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bioRxiv - Immunology 2020Quote: ... after mixing 20 μl of lysate with 100 μl of substrate (Luciferase Assay System-Promega). Relative light units (RLU ...
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bioRxiv - Biochemistry 2021Quote: ... 30 ug of the supernatant was assayed for luciferase activity using luciferase assay substrate (Promega).
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bioRxiv - Cell Biology 2021Quote: ... The detection reagent was prepared by diluting the Nano-Glo Live Cell Substrate (Promega, 0000360026) with the Nano-Glo LCS Dilution Buffer (Promega ...
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bioRxiv - Immunology 2020Quote: ... 100ul of Cell titer-Glo luminiscence ATP reconstituted buffer and substrate (Promega, Cat. No. G7570) was added to each well and Luminiscence was measured after 10 minutes following manufacturer instructions ...
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bioRxiv - Biochemistry 2022Quote: ... the binding solution contains 1.0 µl of NanoLuc substrate stock solution (Promega, Madison, WI, USA). After addition to one well ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 µL/well of the Renilla luciferase substrate (Renilla Luciferase Assay system, cat#E2820, Promega) were then added and luminescence was read approximately two minutes after injection using a Centro XS3 LB960 plate reader (3 s integration) ...
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bioRxiv - Cell Biology 2022Quote: ... Signals were detected by enhanced chemiluminescence (ECL) using the ECL Western Blotting Substrate (#W1015, Promega) or SuperSignal West Pico PLUS (#34577 ...
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bioRxiv - Developmental Biology 2023Quote: ... lysates and luciferase reaction substrates were prepared using the Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with 20 µL 1x Nano-Glo Live Cell Substrate (Promega Cat# N2011) diluted in PBS and Arrestin assays were performed ...
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bioRxiv - Cell Biology 2023Quote: ... Apoptosis activities were detected using substrate from the Caspase-3 Glo Apoptosis kit (Promega, WI).
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bioRxiv - Molecular Biology 2023Quote: ... Alkaline phosphatase (ALP) staining with BCIP/NBT Color Development Substrate solution (#S3771, Promega, Madison, WI) was performed 96 h after the medium replacement as described (42 ...
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bioRxiv - Plant Biology 2024Quote: ... The blot was washed as before and then visualised with Attophos Substrate Kit (Promega, USA) using the Bio-Rad ChemiDoc XRS+ system.
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bioRxiv - Biochemistry 2024Quote: ... plates were placed for 15 min at RT to equilibrate before NanoBRET NanoGlo Substrate (Promega) was added ...
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bioRxiv - Biochemistry 2024Quote: ... and mixed with the nanoluciferase substrate Nano-Glo (Promega cat# N1120, final dilution 1:200) for 2 min before measuring luminescence in a POLARstar OMEGA plate reader (BMG Labtech ...
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bioRxiv - Microbiology 2024Quote: ... at an enzyme-to-substrate ratio of 1:20 and protease max 0.1% (Promega, UK). Digestion was carried out overnight at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μL of the lysate were added to 50 μL luc substrate (Promega Luciferase Assay System). The amount of luciferase was quantified by luminescence (relative luminescence units (RLU)) ...
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bioRxiv - Genomics 2020Quote: ... Cells were lysed and luciferase substrates provided using the Dual-Glo Luciferase Assay System (Promega E2920). Renilla and firefly luciferase activity were measured using the LUMIstar Omega luminometer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... firefly and renilla luciferase substrates were applied using the Dual-Glo Luciferase Assay System (Promega, Mannheim) according to the manufacturer’s manual ...
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bioRxiv - Biochemistry 2021Quote: ... the membrane was imaged using 500 µl of 1:1000 NanoGlo substrate (Promega, catalogue number: N1120) diluted in 10 mM sodium phosphate buffer pH 7.0.
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bioRxiv - Microbiology 2020Quote: ... cells were washes twice and followed by the addition of 50 μL Nano luciferase substrate (Promega). After 5 minutes of incubation at room temperature ...
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bioRxiv - Cell Biology 2021Quote: Substrate RNAs were synthesized by in vitro transcription using a T7 RNA production system (Promega, P1300) in accordance with the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 50 μL of the lysate were added to 50 μL luc substrate (Promega Luciferase Assay System). The amount of luciferase was quantified by luminescence (relative luminescence units (RLU)) ...
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bioRxiv - Cell Biology 2021Quote: ... the transfected cells were lysed and mixed with NanoLuc substrate (Nano-Glo Luciferase Assay System; Promega) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... and 10 μl of each supernatant was added to 50 μl of luciferase assay substrate (Promega). We used a Synergy Neo HTS multimode microplate reader (BioTek ...
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bioRxiv - Systems Biology 2019Quote: ... Luminescence was measured by auto-injecting 50 μL Renilla luciferase substrate (Renilla Luciferase Assay system, Promega) in each well and integrating light output for 4 s using a TriStar luminometer (Berthold ...
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bioRxiv - Neuroscience 2021Quote: ... Substrates, Coelenterazine native (CTZ, NanoLight technology cat. no. 303) and Furimazine (FMZ, Promega cat. no. N1110) were added using a micro-injector connected to the plate reader (BioTek Synergy HT) ...
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bioRxiv - Biochemistry 2020Quote: ... The reporter activity was determined using the dual luciferase substrate system (E1910; Promega, Madison, WI, USA), and normalized with the Renilla luciferase ...
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bioRxiv - Neuroscience 2021Quote: ... 25 uL of lysate was mixed with NanoGlo substrate diluted 1:50 in NanoGlo buffer (Promega) and 25 uL of lysate was mixed with ONE-Glo luciferase assay buffer (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... the blots were developed with Western Blue® Stabilized Substrate for Alkaline Phosphatase (Promega, Madison, WI) for roughly 15 sec ...
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bioRxiv - Microbiology 2021Quote: ... PBS buffer having 1,000 volume diluted Nluc-specific furimazine substrate (Nano-Glo Luciferase Assay System, Promega) was added to each well ...
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bioRxiv - Neuroscience 2021Quote: ... then 50 µL of drug buffer with RLuc substrate coelenterazine h (Promega, 5 µM final concentration) was added per well ...
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bioRxiv - Biochemistry 2022Quote: ... by quickly mixing 2 μL of above refolding mixture with 50 μL of luciferase substrate (Promega). The activity of unheated luciferase in buffer B was set as 100%.
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bioRxiv - Cancer Biology 2023Quote: ... then the firefly luciferase substrate (20 μl) was added (Bright-Glo™ Luciferase Assay Kit, Promega). Light unit values were normalized to the OD600 absorbance of each culture that was measured from the 96-well culture plates ...
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bioRxiv - Biochemistry 2023Quote: ... the membrane was visualized using 500 μL of 1:1000 NanoGlo substrate (Promega, catalogue number: N1110).
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bioRxiv - Biophysics 2023Quote: ... Bioluminescence was measured after addition of the substrate in a GloMax® Discover Microplate Reader (Promega). All experiments were performed thrice in triplicates.
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bioRxiv - Biochemistry 2023Quote: ... HRP (4 μM) was added followed by 20 μL of Nano-glo luciferase substrate (Promega, N1110) to each well ...