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Citations for Promega :
601 - 650 of 1090 citations for SARS Coronavirus Spike Glycoprotein S2 Mosaic C Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Samples were digested atop the filters overnight at 37°C with mass spectrometry grade trypsin (Promega) at a ratio of 1:50 enzyme:protein ...
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bioRxiv - Immunology 2023Quote: ... Three biological replicates for each condition were digested with Lys-C (Alpha Laboratories) and trypsin (Promega) “on beads” as previously described [44] ...
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bioRxiv - Neuroscience 2024Quote: ... Protein samples (15 µg) were digested overnight at 37 °C with trypsin (Promega, Madison, WI-USA) in a protein:trypsin ratio of 50:1 (w/w) ...
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bioRxiv - Neuroscience 2024Quote: ... In-gel digestion was performed overnight at 37 °C with MS grade endo Trypsin/LysC (Promega). The digested peptides were dried using a speedvac and stored at − 20 °C until further processed ...
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bioRxiv - Genetics 2021Quote: ... protein samples were digested overnight at 37°C with 125 ng modified porcine trypsin (Trypsin Gold, Promega). Peptides were extracted under acidic conditions and dried out using a speedvac concentrator ...
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bioRxiv - Microbiology 2021Quote: Peptides from cell-surface/biofilm-ECM-associated proteins were released via a Trypsin/Lys-C mix (Promega) at a final concentration of 50 ng µL-1 ...
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bioRxiv - Microbiology 2021Quote: ... 2 μg RNA was incubated at 30 °C for 2 hr in Spodoptera frugiperda (Sf21) extract (Promega) in the presence of [35S]methionine-cysteine (Perkin-Elmer ...
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bioRxiv - Genomics 2019Quote: ... samples were incubated overnight at 37°C with Tris pH 8.8 and 300 ng Trypsin Gold (Promega). Peptides were desalted using StageTips (Rappsilber ...
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bioRxiv - Biochemistry 2019Quote: ... Protein was digested by incubation overnight at 37°C with trypsin (5 ng/ ml, Promega, Madison, WI) in 50 mM NH4HCO3 (pH 7.8) ...
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bioRxiv - Synthetic Biology 2019Quote: ... ligated for 3 h at room temperature (RT) or overnight at 4°C using T4 ligase (Promega), and propagated in E.coli XL 10 Gold cells (Agilent) ...
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bioRxiv - Cancer Biology 2021Quote: ... Biological triplicates were digested with Lys-C (Alpha Laboratories, Eastleigh, UK) and trypsin (Promega, Madison, WI, USA) on beads as previously described (56) ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were diluted 4-fold prior to overnight digestion at 37 °C with trypsin (Trypsin Gold, Promega) at an enzyme to substrate ratio of 1:50 ...
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bioRxiv - Neuroscience 2020Quote: ... Assay was performed at 37 °C and resorufin fluorescence was measured on GloMax Multi Detection System (Promega) plate reader with an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm.
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bioRxiv - Neuroscience 2019Quote: ... for 16 h at 37°C followed by a second digestion step with 0.15 µg trypsin (Promega) for 4 h at 37°C ...
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bioRxiv - Biophysics 2020Quote: ... The samples were further digested overnight at 37 °C with the addition of sequencing grade chymotrypsin (Promega) to a final protease:protein (w:w ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were digested in-gel using trypsin (Gold Promega, 1 μg per band, overnight at 30°C,) as previously described (Thouvenot et al. ...
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bioRxiv - Microbiology 2022Quote: ... the modified region was amplified using primer SAM_Seq_Gen-23_FW (5’-GAT TTG AGG ACG ACT GGA CTG C) and SAM_Seq_Gen1116_RV (5’-GTC AAC TGA ACA ACC CCA AGG T) together with GoTaq polymerase (Promega), followed by cloning into pGEM T-easy vector system (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... SDS was removed and proteins were first digested with Lys-C (Wako) and subsequently with Trypsin (Promega) with an enzyme to protein ratio (1:50) ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysates were stored at −20°C or used immediately in a Dual-Glo luciferase assay (Promega #E2920) with the SpectraMax ID5 microplate reader ...
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bioRxiv - Neuroscience 2021Quote: DNase treatment was then performed (RQ1 10x buffer, RQ1 DNase by Promega, 30 minutes at 37°C) and stopped by adding 1 μL of DNase Stop Solution (Promega ...
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bioRxiv - Neuroscience 2020Quote: ... Protein digestion was performed overnight at 37 °C in a shaking incubator using 0.12 μg trypsin (Promega) at an enzyme to protein ratio of 1:50 (protein content was calculated using independent samples) ...
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bioRxiv - Immunology 2021Quote: ... and then lung lobes were inflated with 3% (w/v) 45°C prewarmed low-melting agarose (Promega). The inflated lung lobes were immediately removed and cooled on ice for 15 minutes ...
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bioRxiv - Systems Biology 2019Quote: ... Proteins were digested on-filter (16 hr, 37 °C) with sequencing-grade modified trypsin (50:1, Promega). Proteolysis was terminated and peptides eluted with 20 μL of 10% trifluoroacetic acid followed by centrifugation (13,000 ×g ...
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bioRxiv - Microbiology 2019Quote: ... total RNA was treated with RQ1 DNase for 30 minutes at 37°C (Promega, Cat No: PAM6101). The DNase reaction was stopped with RQ1 Stop Solution for 10 minutes at 65°C (Promega) ...
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bioRxiv - Genetics 2020Quote: ... for 3 hours at 37°C in the presence of RNasin Ribonuclease Inhibitor (Promega #N2111, Madison, WI), and PE2 mRNA was purified with MEGAclear™ Transcription Clean-Up kit (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... protein samples were treated with Endoprotease Lys-C (Wako) and Trypsin (Trypsin Gold Mass Spec Grade; Promega). Peptide samples were desalted by OMIX C18 pipette tips (Agilent Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... samples were incubated for 6 h at 37°C with 3 μg Sequencing Grade Modified Trypsin (Promega). Digestion was terminated via addition of 20 μL trifluoroacetic acid (TFA) ...
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bioRxiv - Biochemistry 2021Quote: ... and then cells were pre-incubated 5 minutes at 37°C with 5 μM coelenterazine h (Promega) before adding ligands diluted in PBS supplemented with 0.9 mM CaCl2 and 0.5 mM MgCl2 ...
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bioRxiv - Biochemistry 2021Quote: ... and then cells are pre-incubated 5 minutes at 37°C with 5 μM coelenterazine h (Promega) before adding ligands diluted in PBS supplemented with 0.9 mM CaCl2 and 0.5 mM MgCl2 ...
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bioRxiv - Cell Biology 2020Quote: ... bound proteins were alkylated and digested with endopeptidase Lys-C (Wako) for 3 hours and trypsin (Promega) on beads overnight at 37°C.
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bioRxiv - Biochemistry 2021Quote: ... for 1 h at 37°C (sample to enzyme ratio 1:20) and then with GluC (Promega) (1:40 ratio ...
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bioRxiv - Immunology 2021Quote: ... 50 mM Tris-Cl (pH 8) prior to digestion with Trypsin/Lys-C Mix (Promega, Madison, WI) at a ratio of 1:25 (enzyme ...
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bioRxiv - Cell Biology 2020Quote: ... DNA was prepared from bacterial cultures grown at 37 °C using PureYield™ Plasmid Midiprep System (Promega) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... and samples were digested over night with Lys-C (Wako) followed by 4h digestion with Trypsin (Promega) at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... the samples were incubated overnight at 37°C with 0.01 μg/μL of trypsin (Promega, Madison, WI) and dissolved in 50 mM ammonium bicarbonate ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were reduced and alkylated followed by overnight digestion at 37°C using LysC / Trypsin proteases (Promega) and isobaric labelling using tandem mass tags (TMT ...
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bioRxiv - Bioengineering 2023Quote: ... samples were incubated overnight at 37°C with sequencing grade modified trypsin (0.01 mg/mL; Promega Corporation) to allow protein digestion ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested in-gel overnight at 37°C with Sequencing Grade Modified Trypsin (Promega; cat. #V5111) diluted in 200uL of digestion buffer (40 mM ammonium bicarbonate ...
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bioRxiv - Microbiology 2023Quote: ... the modified region was amplified using primer SAM_Seq_Gen-23_FW (5’-GAT TTG AGG ACG ACT GGA CTG C) and SAM_Seq_Gen1116_RV (5’-GTC AAC TGA ACA ACC CCA AGG T) together with GoTaq polymerase (Promega), followed by cloning into pGEM T-easy vector system (Promega ...
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bioRxiv - Systems Biology 2023Quote: ... then proteins were digested with 1 μg of mass spectrometry grade Trypsin/Lys-C enzyme mix (Promega) in 50 μL of digestion buffer (100 mM ABC ...
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Pan-tissue mitochondrial phenotyping reveals lower OXPHOS expression and function across tumor typesbioRxiv - Biochemistry 2023Quote: ... 1mM CaCl2 for overnight digestion via trypsin at 32°C (Promega, Cat# V5113; 1:50 w:w enzyme:protein). Following digestion ...
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bioRxiv - Cell Biology 2023Quote: ... digested overnight at 37°C in the presence of 1.5 µg of sequencing grade modified trypsin (Promega). The resulting peptides were vacuum-dried in a vacuum concentrator to approximately 200 µL ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were digested overnight (21 h) at 37 °C with 0.1 μg/μL trypsin (sequencing grade, Promega) dissolved in 1 mM HCl ...
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bioRxiv - Molecular Biology 2023Quote: ... on-column trypsin digestion was performed by adding by mass spectrometry grade trypsin/Lys-C mix (Promega) in a 50 mM NH4HCO3 buffer overnight at 37 °C.
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bioRxiv - Pathology 2024Quote: ... at a 1:50 wt:wt ratio alongside Lys-C at a 1:25 wt:wt ratio (Promega, VA1170) at 37 °C for 16 h ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were then subjected to digestion with LysC (Wako) at 37°C for 2h and trypsin (Promega) overnight at 37°C at a 1:50 enzyme:protein ratio ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The samples were further digested overnight at 37 °C with the addition of sequencing grade chymotrypsin (Promega) to a final protease:protein (w:w ...
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bioRxiv - Genetics 2024Quote: ... then a 5 min final extension (72 °C)—was carried out with GoTaq Green Master Mix (Promega). Deletion loci from mutant flies were amplified using the genotyping primers above with four GoTaq Green PCR reactions per line ...
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bioRxiv - Cell Biology 2024Quote: ... alkylated with 10 mM iodoacetamide and subject to sequential protein digestion with Lys-C and trypsin (Promega). Digestion was halted with formic acid ...
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bioRxiv - Genomics 2024Quote: ... We amplified the Cytochrome c oxidase subunit I (COI) for individual abdomen DNA using the GoTaq (Promega) protocol ...