Labshake search
Citations for Promega :
501 - 550 of 1090 citations for SARS Coronavirus Spike Glycoprotein S2 Mosaic C Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... The samples were digested overnight at 37°C with sequencing-grade modified trypsin (Promega) at an enzyme to protein ratio of 1:40 ...
-
bioRxiv - Microbiology 2023Quote: ... followed by digestion with benzonase at 37 °C for 1 h and trypsin (Promega) digestion overnight at the same temperature ...
-
bioRxiv - Cell Biology 2023Quote: ... after which samples were digested overnight at 37 °C with trypsin (Promega, Fitchburg, WI,) in a 1:60 trypsin to protein ratio ...
-
bioRxiv - Cell Biology 2023Quote: ... 1 μL of buffer containing 35 mM iodoacetamide and Trypsin/Lys-C (Promega, V5072) was added to each sample ...
-
bioRxiv - Biochemistry 2023Quote: ... 1 μL of buffer containing 35 mM iodoacetamide and Trypsin/Lys-C (Promega, V5072) was added to each sample ...
-
bioRxiv - Synthetic Biology 2023Quote: ... or zero min at 37°C before addition of Nano-Glo substrate (Promega N1110) in 80 µL ...
-
bioRxiv - Cell Biology 2023Quote: ... The samples were then diluted to 3M urea and digested with Lys-C (Promega) for 4 h at 25°C ...
-
bioRxiv - Biochemistry 2024Quote: ... The NanoLuc coding sequence including linker was obtained from the pNLF1-C plasmid (Promega) and inserted at the C-terminus of the TDO2 gene sequence (Flash Therapeutics ...
-
bioRxiv - Cell Biology 2024Quote: ... biological replicates (n=7) were digested with Lys-C (Alpha Laboratories) and trypsin (Promega) on beads as previously described (Hubner et al. ...
-
bioRxiv - Cancer Biology 2024Quote: ... for 10 minutes at 37°C in the presence of RNasin Ribonuclease Inhibitor (Promega). DNase-treated RNA was purified with the RNA Clean and Concentrator-25 Kit (Zymo Research ...
-
bioRxiv - Cell Biology 2020Quote: ... proteins on the beads were digested by adding 400 ng trypsin/Lys-C mix (Promega) for 16 h at 37°C ...
-
bioRxiv - Cell Biology 2020Quote: ... the samples were digested overnight at 37°C with 1:100 (w/w) trypsin (Promega). The next day ...
-
bioRxiv - Genetics 2021Quote: ... then a 5 min final extension (72 °C)—using GoTaq Green Master Mix (M7123, Promega) and the pCFD6_seqfwd and pCFD6_seqrev primers (Table S1 ...
-
bioRxiv - Biophysics 2022Quote: ... BRET measurements were performed at 37°C by the addition of furimazine (Promega, Wisconsin, USA) at a dilution of 1:200 ...
-
bioRxiv - Genomics 2020Quote: ... and in-gel digested overnight at 37°C with sequencing grade trypsin (Promega Cat# V5111). The peptides were then extracted with extraction buffer (50% acetonitrile (Fisher Chemical Cat#A955-4) ...
-
bioRxiv - Genetics 2019Quote: ... following manufacturer’s protocol at 50°C in the presence of 2 U/μl RNasin (Promega). RT negative controls in which the reverse transcriptase enzyme was omitted were set up in parallel ...
-
bioRxiv - Biochemistry 2019Quote: ... Plates were incubated for 30 min at 25 °C before NanoBRET Nano-Glo Substrate (Promega) was added to both control and experimental samples at a final concentration of 10 µM ...
-
bioRxiv - Microbiology 2021Quote: ... Proteins were digested overnight at 37 °C using trypsin digestion (Sequencing Grade Modified Trypsin, Promega) at pH 8.0 using a protein to protease ratio of 100:1 ...
-
bioRxiv - Biophysics 2021Quote: ... The purified RNA was treated for 30 min at 37°C with RQ1 DNase (Promega) according to the manufacturer’s protocol and then purified using one round of each phenol/chloroform and chloroform extraction followed by precipitation using ethanol in presence of 300 mM sodium acetate pH 5.5 and GlycoBlue coprecipitant (Thermo Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Resuspended grindate was incubated (4°C, 20 min) with 660 U of DNase I (Promega). Insoluble cell debris was pelleted by spinning (16,000 g ...
-
bioRxiv - Biophysics 2021Quote: ... Cells were then incubated overnight at 4 °C with anti-p75NTR (Promega; G323A; 1:300) and anti-TRADD (Santa Cruz ...
-
bioRxiv - Neuroscience 2021Quote: ... proteins were subjected to on-filter proteolysis with Lys-C (Wako Chemicals) and trypsin (Promega). Tryptic peptides were eluted by centrifugation and acidified with trifluoroacetic acid prior to liquid chromatography-mass spectrometry (LC-MSMS ...
-
bioRxiv - Microbiology 2021Quote: ... Samples were digested overnight at 37 °C with 5 µg sequencing-grade modified trypsin (Promega). The samples were then acidified with trifluoroacetic acid (TFA ...
-
bioRxiv - Systems Biology 2020Quote: ... Treated proteins were digested overnight at 37 °C using sequencing-grade trypsin (Promega, cat: V511C) at an enzyme-to-protein ratio of 1:100 ...
-
bioRxiv - Microbiology 2021Quote: ... The concentrates were treated for 30 min at 37°C with 10μL of DNAse (Promega) and 2,5μL of RNAse (Macherey-Nagel ...
-
bioRxiv - Cell Biology 2020Quote: ... Cultures were then incubated overnight at 4°C with either anti-β3-tubulin (Promega #G712A) or anti-Oct4 (Santa Cruz Biotechnologies #SC-5279 ...
-
bioRxiv - Cell Biology 2020Quote: ... and incubated overnight at 37°C with 0.5 µg of sequencing grade modified trypsin (Promega). Samples were acidified using 1% TFA and desalted using 200 µL StageTips packed with three Empore™ SPE Disks C18 (3M ...
-
bioRxiv - Molecular Biology 2022Quote: ... the samples were incubated with a trypsin/lys-C mixture (w:w = 1:50; Promega, USA) at 37°C for 16 h and then the digest reaction was quenched with 1% formic acid (Fisher Scientific ...
-
bioRxiv - Biophysics 2022Quote: ... In-gel proteolytic digestion was performed overnight at 37°C using sequencing-grade trypsin (Promega). The resulting peptides were extracted at room temperature with 50% acetonitrile/0.1% trifluoroacetic acid ...
-
bioRxiv - Developmental Biology 2022Quote: ... and incubated at 37° C for 30 minutes with RQ1 RNase-Free DNase (Promega, M6101). cDNA synthesis was performed using random primers (Promega ...
-
bioRxiv - Neuroscience 2020Quote: ... After two days incubation at 37°C cell viability was assessed using CellTiterGlo (Promega, G7570) following manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: ... The proteins were digested overnight at 37 °C using sequencing grade trypsin (100 ng; Promega) in a buffer containing 25 mM 4-ethylmorpholine acetate and 5% MeCN ...
-
bioRxiv - Cell Biology 2021Quote: ... Proteins on the beads were digested with 200 ng trypsin/Lys-C mix (Promega, V5072) for 16 h at 37°C ...
-
bioRxiv - Cell Biology 2021Quote: ... Neurons were then incubated overnight at 4° C with anti-p75NTR (Promega; G323A; 1:500) and anti-TRAF6 (Santa Cruz ...
-
bioRxiv - Microbiology 2021Quote: ... 4°C) and then resuspended in 100 µl 1 x Passive Lysis Buffer (PLB, Promega). 45 µl of the luciferase assay substrate dissolved in Luciferase Assay Buffer II was plated on a 96-well plate and 10 µl of the cell lysate was added immediately before measurement of Fluc activity using a Tecan reader ...
-
bioRxiv - Microbiology 2022Quote: ... Neutravidin-bound proteins were digested on bead with 400 ng Trypsin/Lys-C mix (Promega) at 37 °C overnight with shaking ...
-
bioRxiv - Systems Biology 2022Quote: ... 500 µg of proteins were digested with trypsin/Lys-C mix (V5071 or V5072, Promega) overnight at 37 °C with a 1:50 enzyme to protein ratio ...
-
bioRxiv - Immunology 2022Quote: ... Proteolytic digestion was initiated by the addition of 250 ng of endoproteinase Lys-C (Promega) and incubating at room temperature for 2 hours ...
-
bioRxiv - Plant Biology 2022Quote: ... Proteins were digested overnight at 37°C using 500 ng of trypsin (Trypsin Gold, Promega). Supernatants were kept and peptides remaining in gel pieces were further extracted with 1% (v/v ...
-
bioRxiv - Cell Biology 2019Quote: ... RT) and sequentially digested with Lys-C (Wako) (o/n at RT) and trypsin (Promega) (6 h at 37 °C) ...
-
bioRxiv - Cell Biology 2019Quote: ... Samples were digested at 37°C overnight in 10 μg MS-grade Trypsin Gold (Promega). Sequencing grade trifluoroacetic acid (TFA ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins bound to beads were digested with 1 μg of Trypsin/Lys-C mix (Promega) for 3 h at 37 °C and then supplemented with and additional amount of 150 ng Trypsin/Lys-C for overnight digestion on an end-over-end rotation apparatus at 37 °C ...
-
bioRxiv - Biochemistry 2021Quote: ... Proteins were digested overnight at 37 °C by addition of 1 µg of trypsin (Promega). Thereafter ...
-
bioRxiv - Molecular Biology 2021Quote: ... In-solution protein digestion was performed using Lys-C (Wako Chemicals, Neuss) and trypsin (Promega). For analysis of the input samples ...
-
bioRxiv - Biochemistry 2021Quote: ... selections were performed using biotinylated c-CDCP1-Fc captured on SA-coated magnetic beads (Promega). Prior to each selection ...
-
bioRxiv - Cancer Biology 2020Quote: ... rat C/EBP□-LIP or -LAP containing pcDNA3 or pSV2Zeo vector by using FugeneHD (Promega) according to the manufactures protocol ...
-
bioRxiv - Cell Biology 2021Quote: Proteins on beads were digested overnight at 37°C with trypsin (Promega, Madison, WI, USA) in a 25-mM NH4HCO3 buffer (0.2µg trypsin in 20µL) ...
-
bioRxiv - Developmental Biology 2022Quote: ... The 50μg of proteins were then trypsin digested overnight at 37 °C (1:25; Promega) using the filter aided sample preparation (FASP ...
-
bioRxiv - Biochemistry 2022Quote: ... smegmatis Complex I (8 μg) was digested with MS-grade trypsin/Lys-C mix (Promega) at a 25:1 (w/w Complex I:protease ratio in 25 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2022Quote: ... 1B and transferred to 37°C immediately after addition of supercoiled pGemT target DNA (Promega). Reactions were incubated for 30 ...