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Citations for Promega :
601 - 650 of 786 citations for Mouse Anti MERS Coronavirus Spike S1 Antibody 3872 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Secondary antibodies used in Western blotting were conjugated to alkaline phosphatase (Promega) or horseradish peroxidase.
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bioRxiv - Microbiology 2023Quote: ... followed by horseradish peroxidase-conjugated (HRP) secondary rabbit antibodies (Promega, 1/25000) and revealed by chemiluminescence (Pierce ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibody signal was detected using the ECL Western Blotting Substrate (W1015, Promega) and X-ray film (F-BX810 ...
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bioRxiv - Neuroscience 2023Quote: ... The immunoblots were probed with appropriate secondary antibodies (Promega and Li-Cor) and visualised by chemiluminescence (Supersignal detection kit ...
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bioRxiv - Developmental Biology 2024Quote: ... the membrane was incubated in secondary antibody conjugated to horseradish peroxidase (Promega) at a dilution of 1:5000 for 1h at room temperature ...
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bioRxiv - Physiology 2020Quote: ... The mouse Bdh1 promoter from −720 bp to +130 bp subcloned into the pGL3-basic luciferase vector (Promega) was kindly given by Dr ...
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bioRxiv - Immunology 2023Quote: ... 2 μg RNA was used to synthesize first-strand cDNA using Moloney mouse leukemia virus (M-MLV) (Promega). The amplification of specific PCR products was detected using SYBR Premix Ex TaqTM II (Vazyme) ...
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bioRxiv - Cancer Biology 2024Quote: RNA from mouse tissue and from cell lines was extracted using the Maxwell RSC simplyRNA Cells Kit (Promega) on the Maxwell RSC instrument according to the manufacturer’s instructions.
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bioRxiv - Genomics 2022Quote: ... followed by incubation with horsradish peroxidase (HRP)-conjugated secondary antibodies (Promega, Madison, WI). The chemiluminescence signals were detected using a Western blot developing machine (Fusion Solo S chemiluminescence imager ...
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bioRxiv - Genomics 2024Quote: ... Membranes were then incubated with 1:10000 HRP-conjugated secondary antibody (Promega #W402B) in TBST + 5% milk for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibody against HaloTag® (G9211; 1:1000) was purchased from Promega (UK).
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bioRxiv - Plant Biology 2020Quote: ... anti-HaloTag monoclonal (Promega, USA, Catalog No. G921A), anti-His-tag mouse monoclonal (Abmart ...
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bioRxiv - Cell Biology 2020Quote: ... and anti-rabbit IgG (711-035-152, Promega). Protein expression levels were quantified with ImageJ software and analysis revealed the ratio of the protein of interest related to a housekeeping gene (actin ...
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bioRxiv - Developmental Biology 2020Quote: ... Rabbit anti-p-JNK (1:50, Promega, V7932), Rat anti-E-cad (1:50 ...
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bioRxiv - Physiology 2019Quote: ... anti-pJNK polyclonal from rabbit (Promega, Mannheim, Germany), Alexa Fluor 488-labeled goat anti-mouse IgG (1:300 ...
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bioRxiv - Immunology 2021Quote: ... 3) goat anti-human H+L (Promega, W403B) at 1:3,000 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... while horseradish peroxidase (HRP)-conjugated anti-rabbit (Promega), Alexa Fluor 555 goat anti-rabbit (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... Anti-rabbit IgG (H+L) HRP Conjugate (Promega) was used as the secondary antibody ...
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bioRxiv - Neuroscience 2019Quote: ... and goat anti-rabbit IgG (1:10,000; Promega) antibodies were used as secondary antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... and Anti-Rabbit IgG (H+L) Conjugate (Promega).
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A rare variant on a common risk haplotype of HFE causes increased risk of hereditary hemochromatosisbioRxiv - Genetics 2019Quote: ... or a secondary anti-rabbit HRP (Promega #W4011) and anti-mouse HRP (Promega W-4021 ...
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bioRxiv - Neuroscience 2021Quote: ... anti-HaloTag® (1:1000) (Promega; G9211; RRID:AB_2688011). Secondary antibodies were ...
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bioRxiv - Physiology 2022Quote: ... either goat anti-rabbit (Promega, Madison, WI, US) at 1:3000 dilution or goat anti-mouse (Agilent Technologies ...
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bioRxiv - Plant Biology 2022Quote: ... incubated in anti-rabbit-HRP (1:5000, Promega) and washed in 3x 10min PBS Tween 0.5% before detection ...
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bioRxiv - Biophysics 2023Quote: ... followed by incubation with anti-HaloTag mAb (Promega) at a 1:2000 dilution in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-chicken IgY (1:1,000; G1351, Promega) and goat anti-mouse IgG (1:5,000 ...
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bioRxiv - Developmental Biology 2021Quote: ... and mouse cyclin B1 were generated using the Riboprobe in vitro Transcription Systems kit according to the manufacturer’s protocol (Promega). Anti-BrdU antibodies were conjugated with protein A-sepharose beads overnight at 4C (GE Healthcare) ...
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bioRxiv - Molecular Biology 2020Quote: ... whereas Bmal1-luc was constructed by inserting the mouse Bmal1 promoter (−779 to +127 in reference to the Bmal1 TSS) to pGL4.11[luc2P] (Promega).
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bioRxiv - Molecular Biology 2020Quote: ... Per2E2-luc reporter gene was constructed by inserting the mouse Per2 promoter (−83 to +156 in reference to the Per2 TSS) into pGL4.12[luc2CP] (Promega), whereas Bmal1-luc was constructed by inserting the mouse Bmal1 promoter (−779 to +127 in reference to the Bmal1 TSS ...
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bioRxiv - Cell Biology 2019Quote: ... was amplified from mouse genomic DNA and cloned upstream of luciferase cassette in KpnI/HindIII site of pGL4.23 (Promega) using primers listed in Supplementary table 1 ...
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bioRxiv - Neuroscience 2022Quote: ... wherein the 3’ UTR of Prr7 (NM_0010302964) was amplified from mouse DNA and cloned into the pmiRGLO dual-luciferase expression vector (Promega). Mutations in miRNA binding sites conserved across mammals were introduced by site-directed mutagenesis using Pfu Plus! DNA Polymerase (Roboklon).
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bioRxiv - Cell Biology 2022Quote: Total amounts of free cholesterol and cholesterol esters were determined in mouse islet lysates as per the manufacturer’s instructions (Promega). Phosphatidylserine levels were measured in mouse islet lysates as per the manufacturer’s instructions (LSBio).
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bioRxiv - Cell Biology 2020Quote: ... Isolated RNA (500ng) was used for cDNA synthesis with mouse Maloney leukemia virus reverse transcriptase (Promega, Madison, WI, USA). SYBR Green RT-qPCR gene expression assays were performed in triplicate in an ABI StepOne Plus thermocycler using appropriate endogenous controls (Applied Biosystems ...
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bioRxiv - Neuroscience 2019Quote: ... Genomic DNA was isolated from tail snip samples with Maxwell® Mouse Tail DNA Purification Kit (Promega, Madison, WI). Polymerase chain reaction (PCR ...
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bioRxiv - Neuroscience 2019Quote: ... and mouse Cbln2 E2 fragments were PCR-amplified from genomic DNA of individual species and inserted into pGL4.24 vector (Promega). The sequences of the PCR primers and synthetic oligonucleotides are listed in Supplementary Table 1.
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bioRxiv - Genetics 2019Quote: ... genes were isolated from C57BL/6 mouse genomic DNA by standard PCR and inserted into the pGL4.10 vector (Promega).
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bioRxiv - Neuroscience 2023Quote: ... A 9.5 kb fragment containing upstream promoter region of mouse Frizzled10 (Fzd10) gene was subcloned into pGEM vector (Promega). CreERT2gene (Feil et al. ...
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bioRxiv - Biophysics 2022Quote: ... Blots were incubated with secondary antibodies for 1h at room-temperature (1:10000 in 5% skim-milk powder, anti-rb-HRP-conjugate (Promgea, W401B)/anti-ms-HRP-conjugate (Promega, W402B)) ...
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bioRxiv - Cell Biology 2019Quote: ... The following primary antibodies against the antigens were used: Tuj-1 (1:300, Promega), Synaptophysin (1:400 ...
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bioRxiv - Cancer Biology 2022Quote: ... After 1h of incubation with a horseradish peroxidase-conjugated secondary antibody (1:3,000, Promega), protein bands were visualized using an enhanced chemiluminescence detection kit (Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... USA) at 1:10 dilution and polyclonal HaloTag antibody (Promega Corp. Madison, WI, USA) at 1:500 dilution and FITC-conjugated donkey anti-mouse secondary antibody and Cy3-conjugated goat anti-rabbit secondary antibody (Invitrogen).
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bioRxiv - Microbiology 2023Quote: ... Immunoreactive bands were detected with alkaline phosphatase conjugated secondary antibodies and NBT/BCIP (Promega). Blots shown correspond to representative results of at least 2 independent experiments ...
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bioRxiv - Microbiology 2022Quote: ... washed three times (10 minutes each) with PBST before incubation for 1 h with α-Mouse IgG HRP Conjugate (Promega) secondary antibody (1:10 000 in PBST) ...
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bioRxiv - Neuroscience 2020Quote: 1μg of total RNA from the human and mouse brain specimen was reverse-transcribed using the M-MLV reverse transcriptase (Promega) for efficient synthesis of the first-strand cDNA ...
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bioRxiv - Neuroscience 2020Quote: A DNA fragment bracketing the proximal promoter and transcription start site of the Iqsec3 gene (–280 to +36) was PCR-amplified from mouse genomic DNA and subcloned into the pGL3-Basic vector (Promega). Full-length mouse Npas4 cDNA was subcloned in pcDNA3 (Gibco-Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: ... Total genomic DNA extracted from liver mouse and HEK293 cells were used as templates for PCR-cloning of the mouse/human promoter/intron fragments into the KpnI/MluI sites of pGL3 basic luciferase reporter vector (Promega). The glutamic acid (E ...
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bioRxiv - Cell Biology 2021Quote: ... genomic DNA from Proteinase K-digested mouse toes was extracted using the Wizard SV genomic DNA extraction kit (Promega, A2360). PCR reactions to amplify a fragment containing the target sites were carried out using the forward and reverse primers (Forward primer ...
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bioRxiv - Neuroscience 2022Quote: DNA constructs with different WT and mutated 5’UTRs of mouse Sox2 mRNA were assembled in the psiCheck-2 vector (Promega) including synthetic Renilla luciferase gene (hRluc ...
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bioRxiv - Cancer Biology 2022Quote: ... Mouse DNA was extracted from the ear using the Wizard® Genomic DNA Purification Kit from Promega (Madison, WI, USA), as per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Reporter plasmids were constructed by inserting the mouse Olig2 gene mR2 and mR6 regions identified by the ChIP assays in situ into the pGL3-Basic vector (Promega), respectively ...