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Citations for Promega :
251 - 300 of 786 citations for Mouse Anti MERS Coronavirus Spike S1 Antibody 3872 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... pLJM1-Luc2 used in generating Spike-pseudotyped lentiviruses was generated by cloning Luc2 from pGL4.26 (Promega) into pLJM1-B* (67) ...
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bioRxiv - Cell Biology 2022Quote: The SARS-CoV-2 spike pseudotyped virus activity was determined by bright-glo luciferase assay (Promega). The plate reader detected the luminescence two days post virus infection or without virus infection ...
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bioRxiv - Microbiology 2023Quote: ... the pcDNA3.1+ expression plasmids for spike proteins were co-transfected using Fugene (Promega, Madison, WI, USA) into HEK293T/17 cells ...
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bioRxiv - Developmental Biology 2020Quote: ... The primary antibodies that were used are mouse β-Gal (1:250; Promega), mouse anti-Cora C615.16 (1:400 ...
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bioRxiv - Immunology 2022Quote: ... mixed with Lumit BiT Set 2 (anti-mouse Ab-SmBiT, anti-rabbit Ab-LgBiT, Promega W1220) and added for 90min ...
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bioRxiv - Microbiology 2023Quote: ... Antibodies were visualized by using anti-rabbit immunoglobulin G-alkaline phosphatase secondary antibodies (Promega) and the CDP-star detection system (Roche Diagnostics ...
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bioRxiv - Immunology 2020Quote: ... were used as primary antibodies and horseradish peroxidase (HRP)-conjugated secondary antibodies were from Promega (Rabbit W401B and Mouse W402B), and the substrate ECL was detected by Pierce ECL2 solution (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-mouse IgG (H+L) HRP conjugate (Promega #W4021, 1:10000), anti-rabbit IgG (H+L ...
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bioRxiv - Microbiology 2021Quote: ... and goat anti-mouse IgG-HRP (Promega W4021; 1:5,000 dilution).
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bioRxiv - Developmental Biology 2019Quote: ... mouse anti-β-galactosidase (1:1000, Z3783, Promega, Madison, WI, USA), rabbit anti-β-galactosidase (1:1000 ...
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bioRxiv - Cell Biology 2019Quote: ... end/or donkey anti-mouse 488 (W4021 – Promega, Madison, WI, USA) IgG ...
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bioRxiv - Cell Biology 2019Quote: ... and mouse anti-β-galactosidase (Z3781; Promega, Madison, WI; 1:200). Alexa Fluor 488-conjugated (A21206 ...
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bioRxiv - Microbiology 2020Quote: ... both purchased from Life technologies and (iii) an anti-mouse conjugated with HRP (#W402B) from Promega. For light or electron microscopy ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was treated with anti-mouse IgG HRP conjugate (Promega) and washed 3 times with phosphate buffered saline ...
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bioRxiv - Cell Biology 2022Quote: ... incubated with HRP-conjugated donkey anti-mouse IgG (1:10,000; Promega), and revealed with Super Signal chemiluminescent substrate reagents (Pierce ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-mouse IgG (H+L) HRP conjugate (Promega #W4021, 1:10000), anti-rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2024Quote: ... anti-mouse IgG (H+L) HRP conjugate (Promega #W4021, 1:10000), anti-rabbit IgG (H+L ...
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bioRxiv - Biochemistry 2024Quote: ... and anti-mouse IgG HRP conjugate (W4021; Promega, Madison, WI, USA) as secondary antibodies for 1.5 to 2.0 h at room temperature with gentle shaking ...
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bioRxiv - Biochemistry 2024Quote: ... An anti-mouse IgG-horseradish peroxidase (HRP) conjugate (1:3,000 Promega) was used as secondary antibody ...
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KIF24 controls the clustering of supernumerary centrosomes in pancreatic ductal adenocarcinoma cellsbioRxiv - Cell Biology 2022Quote: ... and amplified PCR products using primers listed in Table S1 were sub-cloned into pGEM-T Easy (Promega). Purified plasmid DNAs were sequenced using M13 primers.
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bioRxiv - Microbiology 2021Quote: ... Final enrichment was achieved with a nuclease digestion using DNase I and S1 nucleases (Promega Corporation, Madison, WI). Enriched dsRNA was shipped overnight on dry ice to Quick Biology (Pasadena ...
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bioRxiv - Microbiology 2020Quote: ... cells were transfected with 500 ng of either HDM-SARS-Spike-delta21 or HDM_Spike_RBD_B7-1 plasmids and 1.5 ng of Transfection Carrier DNA (Promega, E4881) using BioT reagent (Bioland Sci ...
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bioRxiv - Microbiology 2021Quote: ... Spike+ cells were then single cell sorted into 96-well PCR plates containing RNase inhibitor (N2611; Promega) and stored at −80 °C.
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bioRxiv - Cell Biology 2019Quote: ... As secondary antibody a mouse IgG HRP conjugate was used (Promega, Madison, WI, USA). Detection was carried out by using AceGlow (VWR Peqlab ...
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bioRxiv - Molecular Biology 2023Quote: ... Secondary antibodies used included alkaline phosphatase-conjugated anti-rabbit IgG antibody (1:1,000, #S3738, Promega) and anti- mouse IgG antibody (1:1,000 ...
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bioRxiv - Cell Biology 2024Quote: ... Protein detection was done using primary antibody anti-HaloTag monoclonal antibody (1:1,000 dilution, Promega), primary antibody anti-MamE polyclonal antibody (1:3,000 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... Rabbit anti-HaloTag antibody (G9218) was purchased from Promega, and Donkey anti-rabbit IgG (minimal cross reaction to Bovine ...
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bioRxiv - Plant Biology 2021Quote: ... Anti-rabbit IgG horseradish peroxidase-linked antibody (Promega, USA) was used as the secondary antibody at 1:10,000–20,000 dilution ...
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bioRxiv - Immunology 2021Quote: ... incubated with anti-IgY secondary antibody (Promega Madison, USA) for an hour ...
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bioRxiv - Plant Biology 2023Quote: ... antibodies followed by anti-rabbit HRP conjugate (Promega, W4018). Protein loading was visualised by staining membranes with Ponceau S and protein signals were developed using the SuperSignal West Femto chemiluminescence kit (Pierce).
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bioRxiv - Cell Biology 2019Quote: ... Cell signalling) or rabbit anti-TgTOM40 (1:2000, (van Dooren et al., 2016)) antibodies coupled to secondary horseradish peroxidase (HRP)(Promega for mouse and rabbit, Abcam for rat) conjugated antibodies (1:10,000 ...
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bioRxiv - Genetics 2020Quote: ... Founder mice were analyzed by PCR using primers described in Table S1 and PCR products were subcloned in pGEM.T Easy Vector for sequencing (Promega). F1 and F2 mice were confirmed by PCR and by PCR products sequencing (primers available in Table S1) ...
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bioRxiv - Plant Biology 2021Quote: ... LaMATE2ORF sequence was cloned in pGEM-T easy vector (for primers see Table S1, Promega Italia Srl, Milan, Italy) and deposited in the NCBI database (KY464927).
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bioRxiv - Genetics 2024Quote: ... Existing plasmids constructs of variant-centered test elements for the five variants of interest (see Dataset S1 for genomic regions) cloned in the pGL4.23 vector (Promega) were used as the initial template in PCR ...
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bioRxiv - Genomics 2021Quote: ... polyclonal anti-mouse IgG-HRP raised in goat (W402B, 1:3,000; Promega) and polyclonal anti-rabbit IgG-HRP raised in goat (R1364HRP ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by incubation by secondary goat HRP-conjugated anti-mouse IgG (Promega). Proteins were visualized using chemiluminescence detection reagent (ECL ...
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bioRxiv - Biophysics 2022Quote: ... and a goat anti-mouse tag: HRP 0.1 μL/mL (W402B - Promega).
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bioRxiv - Microbiology 2021Quote: ... were co-transfected with 0.5 μg of spike expression plasmids in pCDNA3 using Fugene 6 and following the manufacturer’s instructions (Promega). Cell-cell fusion was measured using an Incucyte and determined as the proportion of green area to total phase area ...
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bioRxiv - Microbiology 2023Quote: ... and 2 μg of the spike SARS2 (D614G)-pCAGGS (Medicines & Healthcare Products Regulatory Agency CFAR100985) using FugeneHD (Promega) transfection reagent at a ratio of 1:3 DNA:Fugene in optiMEM (Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: A volume of 250 μl fraction was mixed with 500 pg of luciferase RNA spike-in (Promega #L4561) and lysed in 750 μl Trizol LS (Thermo Fisher #10296028) ...
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bioRxiv - Biochemistry 2023Quote: ... Anti-HiBiT western blot was then performed with 1:5,000 dilution of mouse anti-HiBit (Promega, Clone 30E5) and 1:10,000 dilution of goat anti-mouse-HRP secondary antibody (Invitrogen).
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bioRxiv - Biochemistry 2023Quote: ... Anti-HiBiT western blot was then performed with 1:5,000 dilution of mouse anti-HiBit (Promega, Clone 30E5) and 1:10,000 dilution of goat anti-mouse-HRP secondary antibody (Invitrogen).
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bioRxiv - Plant Biology 2022Quote: ... for 2 h as primary antibody and peroxidase-conjugated goat anti-rabbit antibody (1:10,000; Promega) for 1 h with 5 times 10 min washes in-between the incubations ...
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bioRxiv - Plant Biology 2023Quote: ... bound antibodies were revealed with a peroxidase-linked secondary anti-rabbit antibody (Promega, Madison, WI, USA) and visualized by chemiluminescence (ImmunoStar Zeta ...
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bioRxiv - Microbiology 2020Quote: ... the blots were incubated for 1h with secondary antibodies (mouse antirabbit IgG-HRP, Promega W4018) that was diluted 1:10000 in blocking buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... Quantitative PCR (qPCR) was performed with the primers listed in Table S1 and a GoTaq qPCR Master Mix (Promega, A6002) in a Mastercycler realplex2 thermocycler (Eppendorf) ...
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bioRxiv - Plant Biology 2020Quote: ... and then reverse-transcribed with a cox1 specific primer (Atcox1-1; table S1) using M-MLV (Moloney Murine Leukemia Virus) Reverse Transcriptase (RNase H minus, point mutant; Promega). The RNA template was degraded by adding 1/5 volume of 1 M NaOH to the reaction mixture and incubating at room temperature for 10 minutes ...
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bioRxiv - Microbiology 2022Quote: The upstream regions of genes of interest were amplified by PCR with specific primers (Table S1) and cloned into the pGEM-T easy plasmid (Promega). After digestion with proper restriction enzymes (Table S1) ...
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bioRxiv - Bioengineering 2022Quote: ... the target region of the human HTT promoter was PCR amplified from genomic DNA of HEK293T cells using the primers Gibson-HTT-Luc-Fwd and Gibson-HTT-Luc-Rev (Supplementary Table S1) and inserted between the KpnI and NheI restriction sites of psiCHECK-2 (Promega) by Gibson Assembly using the Gibson Assembly Master Mix (New England Biolabs ...
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bioRxiv - Plant Biology 2021Quote: ... benthamiana cDNA using gene-specific primers listed in Supporting Information Table S1 as a NcoI-KpnI fragment by High Fidelity PCR (Promega) according to the manufacturer’s protocol ...