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Citations for Promega :
601 - 633 of 633 citations for Measles virus lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: Tethering assay in rabbit reticulocyte lysates was performed by applying in vitro transcribed and capped mRNA encoding mApple-YTHDF1N-MCP or mApple-MCP to the lysates (Promega, L4960) for effector protein translation ...
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bioRxiv - Cell Biology 2022Quote: The other in vitro translation was done in the presence of radioactive [35S]methionine using the TNT T7 Reticulocyte Lysate System (Promega 14610). In vitro–translated proteins were then precleared by incubation with empty glutathione Sepharose beads in NETN buffer (50 mM Tris ...
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bioRxiv - Biochemistry 2022Quote: Cell lysates were prepared as per the manufacturers protocol using the CellTiter-Glo® Luminescent Cell Viability Assay kit (Promega: G7570). Briefly ...
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bioRxiv - Biochemistry 2023Quote: 10 μL in vitro nLuc mRNA translation reactions were performed in the dynamic linear range using 3 nM mRNA (31) in the Flexi Rabbit Reticulocyte Lysate (RRL) System (Promega # L4540) with final concentrations of reagents at 20% RRL ...
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bioRxiv - Biochemistry 2023Quote: ... 25 μL diluted lysate was mixed with an equal volume of Renilla-Glo Luciferase Assay System (prepared 1:100 as recommended; Promega # E2710) for 10 min at room temperature.
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bioRxiv - Microbiology 2023Quote: ... The presence of glycosylated proteins in the cell lysates was assessed using Promega Endoglycosidase H (Endo H) assay reagents (Promega V4871). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were generated with pCS2-N-terminal 5×MYC vectors and pCS2-N-terminal 3×HA vectors described above using TnT® Coupled Reticulocyte Lysate System under the SP6 promoter (L4600, Promega) and by following manufacturer’s recommendations with few modifications ...
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bioRxiv - Molecular Biology 2023Quote: 10 μL in vitro translation reactions were performed in the linear range using 3 nM mRNA in the Flexi Rabbit Reticulocyte Lysate (RRL) System (Promega # L4540) with final concentrations of reagents at 30% RRL ...
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bioRxiv - Neuroscience 2023Quote: mRNAs of C9orf72 intron1-(G4C2)80 with a Myc tag in the GA frame were in vitro translated with Flexi® Rabbit Reticulocyte Lysate System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... the luminescence of 50 µl of cell lysate was measured using the Nano-Glo Luciferase Assay kit (Promega, Madison, WI, USA) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... The lysates were generated by adding 100 µL 1X Passive Lysis Buffer included in the Dual-luciferases assay kit (Promega E1960) to the protoplasts and vigorously shaking at room temperature for 15 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... One µL of lysate was then added to make 20µL PCR reactions with GoTaq G2 Green Master Mix (Promega, Cat# M7823) and 1µM forward and reverse primers (Table 1) ...
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bioRxiv - Molecular Biology 2021Quote: ... IPs were performed by incubating lysates equivalent to 20 mg total protein supplemented with RNase inhibitor - RNasin (Promega, Germany, Cat. No. N2511) and 1 mM DTT with 80 μl of anti-GFP magnetic beads GFP-Trap (Chromotek ...
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bioRxiv - Microbiology 2020Quote: ... cells were lysed in 100 μL of passive lysis buffer and 50 μL lysate was incubated with 100 μL of luciferase assay substrate according to the manufacturer’s instructions (Promega, Madison, WI, USA).
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bioRxiv - Cancer Biology 2020Quote: In vitro translation of full-length SREBP1a and full-length SREBP1c was carried out using the TNT rabbit reticulocyte lysate translation kit (Promega, Wisconsin, USA) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... the cells were lysed in 100 μL of passive lysis buffer and 50 μL lysate was incubated with 100 μL of luciferase assay substrate according to the manufacturer’s instructions (Promega, Madison, WI, USA).
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bioRxiv - Biochemistry 2021Quote: ... 20 μl of the cell lysate was used for luciferase reading using 50 μl of Renilla luciferase reagent (Promega, Madison, WI, USA). Relative light units (RLU ...
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bioRxiv - Plant Biology 2022Quote: ... The pCMX-PL2-NterHA/MED14 vector was constructed as described above and expressed as HA-MED14 proteins using the TNT T7 Coupled Reticulocyte Lysate System (Promega, Madison, WI). HA-MED14 prey proteins were incubated with the affinity-purified GST-PIF4 or GST-HMR bait proteins immobilized on glutathione Sepharose 4B beads (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA for PCR was isolated from 50 µl of tissue lysate using the Maxwell RSC tissue DNA Kit (Promega, Madison, WI, USA).
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bioRxiv - Immunology 2023Quote: ... 5% CO2 for 24 hrs, after which luciferase activity (Relative Light Units, RLU) was measured in cell lysates using the Bright-Glo system (Promega, Madison, WI) with a Bio-Tek II plate reader ...
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bioRxiv - Systems Biology 2019Quote: ... the cells were immediately re-plated in 2 ml of complete medium for 24 hours and then lysates were analyzed with the Dual Luciferase reporter system (Promega Catalog No. E1910).
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bioRxiv - Microbiology 2022Quote: ... Cells were then washed in PBS and infectivity was determined by measuring Renilla luciferase activity in the lysates of infected cells (Promega, Madison, WI, USA) using a Glomax luminometer (Promega).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The number of migrated cells was determined by measuring ATP in the lysates of transmigrated cells using a luminescence-based assay (CellTiter-Glo; Promega, Madison, WI, USA) and readouts were taken in the FlexStation 3 Multi-Mode Microplate Reader (Molecular de- vices LLC ...
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bioRxiv - Systems Biology 2023Quote: ... Peptide loading was assessed by comparing the intensity of the total ion current to 200 ng of a human K562 whole cell lysate standard (Promega, Madison, Wisconsin, USA) prepared at 0.2 μg/μL.
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bioRxiv - Cancer Biology 2023Quote: ... cells were lysed and the cell lysates were prepared according to manual of the Dual-Luciferase® Reporter Assay System (Promega, Madison, USA) and measured with the GlowMax navigator microplate luminometer (Promega ...
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bioRxiv - Cancer Biology 2023Quote: ... The β-Galactosidase activity was measured in 30 µl of lysate incubated with 20 µl of reporter lysis buffer and 50 µl of 2X β-gal Enzyme Assay buffer (Promega, Cat# E2000). The absorbance was read in iMark microplate reader (BioRad) ...
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bioRxiv - Molecular Biology 2024Quote: ... as well as pcDNA3.1-eGFP as a control were used as templates for in vitro transcription and translation using the T7 TNT® Coupled Reticulocyte Lysate System (Promega, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... 100 uL of the diluted lysate was added to each well along with an equal volume of Caspase Glo 3/7 assay reagent (Promega, Madison, Wisconsin, USA), according to the manufacturer’s directions ...
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bioRxiv - Biochemistry 2024Quote: ... The wild-type and mutated GCE variants were expressed in vitro using the TnT Quick T7 Coupled translation rabbit reticulocyte lysate system (Promega, Madison, WI, USA). The reaction products were examined in the dextran-coated charcoal binding assay4,9 with own synthesized [3H]JH III (25.7 Ci/mmol).62 The reticulocyte lysate without DNA input was the background control.
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bioRxiv - Developmental Biology 2019Quote: ... In vitro transcription and translation reactions were performed using a TNT T7 coupled reticulocyte lysate system as recommended by the supplier (Promega, Charbonnieres-les-Bains, France). 1 µG of the plasmid DNA template was transcribed and the protein was translated at 30°C for 90 min.
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bioRxiv - Developmental Biology 2019Quote: ... Tagged-β-Catenin and Tagged-β-Catenin mutants were synthesized using the TNT coupled reticulocyte lysate system according to the manual (Promega, L5020, USA). Briefly ...
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bioRxiv - Plant Biology 2022Quote: MYC-tagged AtGID1c or PpGLP1 expressed from pGBKT7 and HA-tagged AtRGA or PpDELLAa or PpDELLAb expressed from pGADT7 (Yasumura et al., 2007) were translated in vitro using the TNT® T7 Coupled Reticulocyte Lysate System (Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 µl of lysate was added to a 1.5 ml Eppendorf tubes containing 100 µl of luciferase assay reagent II (Promega luciferase assay system, Cat# E1500) and the mixture pipetted in and out three times ...