Labshake search
Citations for Promega :
401 - 450 of 660 citations for Measles virus lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2024Quote: ... Luciferase signals from the cell lysate were evaluated using the GloMax 96 Microplate Luminometer (Promega, USA).
-
bioRxiv - Cell Biology 2024Quote: ... Protein lysates from the single cells were digested with 2 ng of Trypsin (Promega, Cat. V5280), dissolved in 1 μl of 100 mM TEAB pH 8.5 containing Benzonase (Sigma ...
-
bioRxiv - Microbiology 2024Quote: ... The luciferase activity of cell lysates was measured using a firefly luciferase assay kit (Promega, USA), according to manufacturer’s instructions ...
-
bioRxiv - Biophysics 2021Quote: ... and were produced by translation in rabbit reticulocyte lysates using TnT Quick Coupled Transcription/Translation System (Promega) (see Supplementary Table 5 for plasmids) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Luciferase activity of the lysates was measured using a Nano-Glo Dual-Luciferase Reporter Assay System (Promega) and SpectraMax Paradigm (Molecular Devices ...
-
bioRxiv - Molecular Biology 2021Quote: Messenger RNAs were translated in nuclease-treated rabbit reticulocyte lysate (RRL) or wheat germ (WG) extracts (Promega). Typical reactions were composed of 90% v/v RRL ...
-
bioRxiv - Cancer Biology 2019Quote: ... Firefly luciferase activity present in cellular lysates were assayed using the dual luciferase reporter system (Promega Corp.), and light emission was quantified using a luminometer (Junior Berlthold).
-
bioRxiv - Genomics 2019Quote: ... We measured the luciferase activity with cell lysates using the Dual-Luciferase® Reporter Assay System (Promega). We normalized Firefly luciferase activity to the Renilla luciferase activity ...
-
bioRxiv - Bioengineering 2021Quote: Target proteins were in-vitro expressed using rabbit reticulocyte lysate (TNT-coupled reticulocyte system; Promega; #L4600, #L4610) supplemented with either 35S-methionine/S-L-cysteine mix (PerkinElmer ...
-
bioRxiv - Synthetic Biology 2021Quote: ... 1ul of yeast lysate was used as template in a 10 µl GoTaq Green reaction (Promega M7123) with 0.25 µM of primers ...
-
Antibody potency, effector function and combinations in protection from SARS-CoV-2 infection in vivobioRxiv - Microbiology 2020Quote: ... and nanoluc luciferase activity in cell lysates was measured using the Nano-Glo Luciferase Assay System (Promega). Relative luminescence units obtained were normalized to those derived from cells infected with SARS-CoV-2 MA pseudovirus in the absence of monoclonal antibodies ...
-
bioRxiv - Microbiology 2021Quote: ... Lysates were used to measure NAD+ and NADH levels using the NAD/NADH-Glo Assay (Promega, G9071) by following the manufacturer’s protocol.
-
bioRxiv - Cancer Biology 2022Quote: ... Lysates were diluted 4-fold with 100 mM ammonium acetate and digested with sequence-grade trypsin (Promega) overnight at RT at an enzyme:substrate ratio of 50:1 (w/w) ...
-
bioRxiv - Microbiology 2020Quote: ... Renilla luciferase activity in the cell lysates was measured using the Rluc system (Promega, Madison, WI, USA) (48).
-
bioRxiv - Microbiology 2021Quote: ... Lysates were further diluted with 50 mM ammonium bicarbonate to < 2M urea and digested with trypsin (Promega) overnight at 37 ⁰C ...
-
bioRxiv - Microbiology 2021Quote: ... and Firefly and Renilla luciferase activities in the lysates were measured using a dual luciferase kit (Promega).
-
bioRxiv - Molecular Biology 2021Quote: ... Lysates were stored at −20°C or used immediately in a Dual-Glo luciferase assay (Promega #E2920) with the SpectraMax ID5 microplate reader ...
-
bioRxiv - Immunology 2020Quote: ... and finally the luciferase activity in total cell lysates was measured with Glomax (Promega, Madison, WI, USA).
-
bioRxiv - Genomics 2019Quote: ... The luciferase activity of cell lysates was measured using a luciferase assay kit (Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... and finally the luciferase activity in total cell lysates was measured with Glomax (Promega, Madison, WI, USA).
-
bioRxiv - Biochemistry 2020Quote: ... In vitro translation assays were run on the prepared mRNA templates using Rabbit Reticulocyte Lysate kits (Promega), with translation mixes prepared in triplicate as per manufacturer′s protocols (for S2D ...
-
bioRxiv - Microbiology 2020Quote: ... and lysates were read on a Promega GloMax Multi Detection unit using Luciferase Assay Reagent II (Promega) following the manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2021Quote: ... 1500 μg total protein lysate was 100ul slurry of pre-cleared with Protein G agarose beads (Promega), followed by overnight incubation with AXL antibody (2ug Ab/ 100 ul lysate) ...
-
bioRxiv - Immunology 2020Quote: ... Firefly luciferase activities were measured in 10 μl of lysate using 50 μl luciferase assay substrate (Promega). To normalise transfection efficiencies β-Galactosidase levels were measured using the β-Galactosidase Enzyme Assay System (Promega) ...
-
bioRxiv - Microbiology 2024Quote: ... the lysates were diluted with 50mM HEPES pH8.0 to <2M urea and digested overnight with trypsin (Promega) at 1:50 (enzyme:protein ...
-
bioRxiv - Immunology 2023Quote: Proteasome activity was measured in cell lysates using the Proteasome-Glo™ 3 Substrate System (Promega, G8531). Corresponding reagents for testing as chymotrypsin-like ...
-
bioRxiv - Immunology 2023Quote: ... and 25 µl cell lysates were mixed with 25 µl Bright-Glo or Nano-Glo (both Promega), and luminescence was measured by a luminometer ...
-
bioRxiv - Cell Biology 2022Quote: Constructs for in vitro translations in rabbit reticulocyte lysate were based on the pSP64 vector (Promega, USA). Constructs for in vitro translation in the E ...
-
bioRxiv - Genomics 2023Quote: ... The Luciferase assay was performed with 100 µL of the cleared lysate (Steady Glo reagent, Promega, E2510) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... The quantification of NADPH was assessed in total cell lysates using the NADP/NADPH Assay Kit (Promega) following the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... The cell lysates were used to determine luciferase activity with the Dual-Luciferase Reporter Assay System (Promega) and a GloMax 96 Microplate Luminometer (Promega) ...
-
bioRxiv - Molecular Biology 2023Quote: ... The Luciferase assay was performed with 100 µL of the cleared lysate (Steady Glo reagent, Promega, E2510) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: The reaction mixture (10 μl) was prepared with 5 μl of rabbit reticulocyte lysate nuclease-treated (Promega), 2 μl of H2O ...
-
bioRxiv - Physiology 2023Quote: ... Cell lysates (20 μL) were assayed for luciferase activity using the dual Luciferase Assay System (Promega, USA), according to manufacture instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... Lysates were analyzed for firefly and Renilla luciferase activities on a GloMax® 20/20 luminometer (Promega), following the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2020Quote: ... and lysate was either measured directly for GFP signal or incubated with luciferase assay reagent LAR (Promega, E1500) for luminescence signal ...
-
bioRxiv - Immunology 2021Quote: ... lysates were transferred to white plates and luminescence was read in a GloMax® Discover Microplate Reader (Promega). Neutralization activity was determined by comparing the signal from the wells infected with virus lacking antibody to the different sera dilutions ...
-
bioRxiv - Developmental Biology 2020Quote: ... After 24 hours luciferase activities were assayed in cell lysates using the Dual-Luciferase Reporter Assay System (Promega) and a multi-label counter (Promega GloMax) ...
-
bioRxiv - Microbiology 2020Quote: ... dengue protein NS2B/3/4A constructs were expressed in rabbit reticulocyte lysate (TNT coupled T7, Promega Bio Systems) programmed with 1 µg DNA/50 µL and labeled with 20 µCi of L-[35S]-methionine (EasyTag ...
-
bioRxiv - Cancer Biology 2019Quote: ... The lysate was quantified for firefly and Renilla luciferase activity using the Dual-Glo Luciferase assay system (Promega) to determine miR-211-dependent target degradation/transcriptional inhibition ...
-
bioRxiv - Biochemistry 2020Quote: ... The diluted lysate was added to 100 µl of pre-equilibrated Magne® HaloTag® Beads (Promega, G7282) and incubated overnight on a rotator at 4 °C ...
-
bioRxiv - Plant Biology 2021Quote: ... Apoproteins of PHYA-HA and PHYB-HA were prepared using a TNT T7 Coupled Reticulocyte Lysate system (Promega) as described previously75 ...
-
bioRxiv - Microbiology 2021Quote: ... 35S methionine-labeled full-length cGAS and E5 was generated with the TnT Coupled Reticulocyte Lysate System (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: ... and cell lysates were prepared and examined by using the Dual luciferase reporter assay system (Promega, Madison, WI), according to the manufacturer’s protocol.
-
bioRxiv - Biochemistry 2022Quote: ... the beads with bound recombinant proteins were blocked for 1 h at 4°C using reticulocyte lysate (Promega). Next ...
-
bioRxiv - Molecular Biology 2020Quote: In vitro transcription/translation of vvl/dfr vDNA was carried out using TNT coupled reticulo lysate system (Promega) and T3 RNA polymerase (New England Biolabs Inc. ...
-
bioRxiv - Molecular Biology 2020Quote: Luciferase activity of cell lysate transfected with pGL3 promoter constructs was detected with the Dual Luciferase Assay (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... 200 µl/sample) and split into fractions with or without MNase (Promega, 50U/100 µl of nuclear lysate) and incubated for 1 hour at 4°C ...
-
bioRxiv - Biochemistry 2020Quote: ... Lep proteins were then produced from mRNA by in vitro translation using rabbit reticulocyte lysate (Promega, Madison, WI) supplemented with canine pancreatic rough microsomes (tRNA probes ...
-
bioRxiv - Molecular Biology 2019Quote: ... NanoLuc and Firefly activities in cell lysates were measured using Nano-Glo Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s protocols.