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Citations for Promega :
6351 - 6400 of 6889 citations for Mouse Translational Activator Of Cytochrome C Oxidase 1 TACO1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Semiquantitative PCR reactions were carried out with 1–2 μL of cDNA and 0.2 μL of GoTaq DNA Polymerase (Promega) in a total reaction volume of 30 μL according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... DTT was added to a final concentration of 3 mM before adding 1 μg of sequencing-grade trypsin (Promega) and incubating at 37 °C overnight ...
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bioRxiv - Genomics 2022Quote: ... Single nuclei were sorted into 384 well plates containing 1 µl M-Digestion Buffer containing proteinase K and ~0.05 pg Lambda DNA isolated from dcm+ E.Coli (Promega #D1501).
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bioRxiv - Genetics 2022Quote: ... Samples were then diluted 10fold with 20 mM Tris and trypsin (Trypsin Gold, Promega, #V5280, 1:200 w/w) and digested overnight at 37°C ...
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bioRxiv - Plant Biology 2022Quote: ... The medium was aspirated and fresh medium containing 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS, premixed in 5:1 ratio, Promega) was added to the treated cells ...
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bioRxiv - Molecular Biology 2022Quote: Edited loci were amplified using locus-specific primer pairs described in Supplementary Table 1 using GoTaq master mix (Promega) and 200ng of genomic DNA ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the cells were preincubated with 50 µl of a mix of Nluc substrate endurazine (1:50 dilution; #N2571, Promega) and LgBiT (1:50 dilution ...
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bioRxiv - Molecular Biology 2023Quote: ... ncBAF (Brd9) and PBAF (Baf180) (supplemental table 1) were isolated by using the Pure yield plasmid midiprep system (Promega) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... RNA reverse transcription and cDNA amplification was then carried out by the GoTaq 1-Step RT-qPCR System (Promega), using established primers (31) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µL per input or ChIP sample was used in a qPCR using the GoTaq® Mastermix (#M7133, Promega) and primer pairs for the amplification of the Ifnb1 promoter region (ChIP_IFNb1_fwd 5’-GCCAGGAGCTTGAATAAAATG ...
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bioRxiv - Microbiology 2022Quote: ... RNA was extracted from leaves or bacterial liquid cultures via phenol/chloroform extraction and treated with DNase 1 (Promega) to remove gDNA contamination ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... in which donor luciferase luminescence was stimulated with the addition of furimazine (1/960 dilution from Promega manufacturer’s stock) in the additional absence or presence of the orthosteric agonist 10μM isoproterenol (final assay volume ...
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bioRxiv - Neuroscience 2022Quote: ... beads were resuspended in elution buffer (50mM Tris, 1mM EDTA, 150mM NaCl, 1% SDS, 50mM NaHCO3, pH 8.1) supplemented with RNasin (Promega) and incubated with proteinase K (New England Biolabs ...
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bioRxiv - Cell Biology 2022Quote: ... were seeded onto 6-well plates coated with 0.1 mg ml−1 poly-L-lysine and transfected using FuGENE HD transfection reagent (Promega) with a mixture of third-generation lentiviral backbone vector pJHUMCS that encodes BDNF-pHluorin ...
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bioRxiv - Cancer Biology 2022Quote: ... Urea concentration across control and sample tubes were dropped to ∼2M with 20 mM HEPES pH 8.0 and were digested with 1 μg sequencing grade trypsin (Promega) overnight at 37°C in a nutator ...
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bioRxiv - Microbiology 2023Quote: ... 300 µl of eluant (at 1.5 mg/ml) was treated with 6.5 µl of 1 mM HaloTag Alexa Fluor 488 Ligand (Promega PAG1001). Both labeled and unlabeled proteins were subject to size exclusion chromatography for further purification using Superose 6 Increase 10/300 GL column (Cytiva ...
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bioRxiv - Biochemistry 2024Quote: pcDNA5/FRT/TO-SFB-HELB (WT, D506G, and 3xA) plasmids (1 µg) were transfected into 1.0 × 105 cells using FuGENE HD (Promega) according to the manufacturer’s protocol followed by incubation at 37°C with 5% CO2 24 hours ...
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bioRxiv - Cell Biology 2024Quote: ... Each sample was digested on beads in 100 µl of 50 mM ammonium bicarbonate with 1 µg trypsin (Promega) in spin cap tubes sitting in fresh mass-spectrometry grade Eppendorf tubes ...
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bioRxiv - Neuroscience 2024Quote: ... were transfected 2 days after plating with 1 μg of total DNA with FuGENE 6 transfection reagent (E2691; Promega) at a ratio 1:3.
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bioRxiv - Microbiology 2024Quote: ... Cells were then transfected with plasmid DNA (4µg per plasmid per flask) using FuGENE HD (1:3 ratio; Promega) and cultured at 28°C.
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bioRxiv - Microbiology 2024Quote: ... At 1 day after the treatment the reporter assay was performed using Dual-Glo Luciferase System (Promega, Cat# E2920) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR product was purified after migration on a 1% agarose TAE 0.5X gel using the Wizard PCR Clean-Up system (Promega) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... 1–5 µl of gDNA was mixed with 0.5 µl of 10 mM dNTP mix (Promega, C1141, Madison, WI), 10 µl of 25 mM MgCl2 ...
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bioRxiv - Cell Biology 2024Quote: ... reverse transcription was carried out using 1 μg of total RNA and random primers (Reverse Transcription System, Promega, A3500). IQTM SYBR® Green qPCR Supermix (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... proteolytic digestion was performed by adding Trypsin (0.1 μg/μL in 1 mM HCl, Sequencing Grade Modified Trypsin, Promega) at a ratio of 50:1 (w:w ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed once in PBS and lysed in 35 μL 1× Luciferase Cell culture lysis buffer (E1531, Promega) for 15 min at room temperature and centrifuged for 10 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... A maximum of 500 ng of RNA was mixed with 1 μL RQ1 10x Buffer (Promega, cat. no: M6101) and 1 μL RQ1 DNase (1 U/μL ...
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bioRxiv - Synthetic Biology 2022Quote: ... CFPS reactions were run as described above with the addition of 1 μl of FluoroTect™ reagent (Promega L5001) per 50 μl CFPS reaction ...
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bioRxiv - Neuroscience 2022Quote: ... HEK cells were transfected with the targeting plasmid and two helper plasmids (psPAX2 and pMD2.G) at a 2:1.5:1 molar ratio using ProFection (Promega). Two to three days later ...
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bioRxiv - Genetics 2022Quote: ... membranes were washed and incubated with the appropriate HRP conjugated secondary antibody (1:3,000 anti-rabbit W401B antibody from Promega), and the reaction was finally visualized with the Western Blotting Luminol Reagent (Santa Cruz Biotechnology) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1/20th of the resulting cDNA was used then analyzed by qRT-PCR using GoTaq qPCR master mix (Promega). The following primer pairs were used:
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bioRxiv - Systems Biology 2023Quote: ... Samples were diluted 1:3 with 50 mM HEPES pH 8.5 and then digested by a 1:50 (trypsin to protein) ratio of sequencing grade modified trypsin (Promega) for 16 hours at 600 rpm and 25°C ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins captured on the columns were then digested by adding 20 μL of digestion buffer (50 mM TEAB, pH 8.5) containing 1 μg of sequencing grade trypsin (Promega) 1 hour at 47
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bioRxiv - Evolutionary Biology 2023Quote: ... 3 nM of either XeARPP19 or various forms of ClyARPP19 (stock solutions at 1 μg/μL) were incubated in the presence of 62.5 units of recombinant bovine PKA (Promega) and 1 mM γS-ATP in a final volume of 30 μL of PKA Buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... 100 ng of purified genomic DNA was spiked with 1 ng of unmethylated lambda DNA (Promega, Madison, WI, USA) and subjected to bisulfite treatment using the EZ DNA methylation gold kit (Zymo Research ...
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bioRxiv - Neuroscience 2023Quote: ... centrifuged and resuspended in EZ lysis buffer, followed by three washes in wash/resuspension buffer (1x PBS, 1% BSA, 0.2U/μL RNase Inhibitor (Promega)) and labeled with propidium iodide (PI ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed 3 times for 10 min in TBS-T and subsequently incubated with species-specific HRP-conjugated secondary antibodies (1:10,000; Promega) in 5% non-fat dry milk -TBS-T for 1 h at room temp ...
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bioRxiv - Genetics 2023Quote: ... samples were suspended in homogenization buffer (50mM Tris, pH7.5, 100mM KCl, 12mM MgCl2, 1%NP40-substititue, 1mM DTT, 200U/mL Promega RNasin ...
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bioRxiv - Microbiology 2023Quote: ... and 400-800 ng of end repaired gDNA using a 1:20 dCTP:ddCTP mixture (9.5 mM dCTP Millipore Sigma #3732738001, 0.5 mM ddCTP Promega #U1225) to generate ∼20 bp C-tails.
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bioRxiv - Immunology 2023Quote: ... and digested for 4 hrs with 2 mg/ml LysC (Wako Chemicals GmbH) in a ratio of 1:50 and O/N with 0.1 µg/µl trypsin (Promega) in a ratio of 1:50 ...
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bioRxiv - Biochemistry 2023Quote: ... 10 µL of 1:100 (diluted in phenyl red-free DMEM with 4% FBS) Nano-Glo substrate (Promega N1572) was added in each well ...
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bioRxiv - Developmental Biology 2023Quote: ... with 1 ug of RNA and RT-qPCR reactions were performed using GoTaq® qPCR Master Mix (A6002 - Promega). Analyses were carried out in the QuantStudio™ 5 Real-Time PCR System ...
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bioRxiv - Cancer Biology 2023Quote: ... 170 µL medium was removed and 30 µL caspase 3/7 reaction (1:100 Z-DEVD-R110 fluorogenic substrate:Homogenous buffer; ApoONE, Promega) was added to each well ...
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bioRxiv - Cell Biology 2022Quote: ... The gel pieces were rehydrated in trypsin solution (50 mM ABC pH 8.0, 1 or 2 µg trypsin per sample for proteome or BioID respectively, Promega) overnight at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were diluted 3 fold with 20mM HEPES pH 8.0 and digested in 10 @g ml-1 trypsin-TPCK (Promega) overnight at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... The milk solution was removed and the membrane was incubated with a 1:1,000 dilution of streptavidin-alkaline phosphatase antibody (Promega) in TBS-T for 1 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Then the beads were incubated with 80 μL of 2 M urea in 50 mM Tris-HCL containing 1 mM DTT and 0.4 μg trypsin (Promega) at 25 °C for 1 hour while shaking at 1,000 rpm ...
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bioRxiv - Biophysics 2023Quote: ... In the first round of selection 400 nM of Xl-HAS-1-loaded nanodiscs diluted in the selection buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 1% BSA) was immobilized on streptavidin paramagnetic beads (Promega). Beads were washed three times in the selection buffer ...
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bioRxiv - Immunology 2023Quote: ... the cells plus supernatants were combined with an equal volume of the respective caspase-Glo reagent for 1 h according to the manufacturer’s instructions (Promega). The mixture was then transferred to a Lumitrac 600 plate and luminescence was measured using a SpectraMax i3 instrument and the SoftMax Pro 6.3 Software (Molecular Devices) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... CP4 medium was exchanged with Hanks’Balanced Salt Solution (with added glucose 1 g/l, NaHCO3 0.35 g/l) containing GloSensor cAMP Reagent (3 %, Promega) and plates were incubated for 60 min ...